Biochemical analysis on microfluidic chips

TrAC - Trends in Analytical Chemistry

Volumn 80, Issue , 2016, Pages 213-231

  Wu, Jing ^a   He, Ziyi ^b   Chen, Qiushui ^b   Lin, Jin Ming ^b  

^a CHINA UNIVERSITY OF GEOSCIENCES   (China)

^b TSINGHUA UNIVERSITY   (China)

Author keywords

Acoustic wave; Biochemical analysis; Biomimetics; Biomolecular analysis; Cell analysis; Chip MS platform; Electronic methods; Magnetic operation; Microfluidic chip; Optical detector

Indexed keywords

ACOUSTIC WAVES; BIOMIMETIC PROCESSES; BIOMIMETICS; FLUIDIC DEVICES; STEM CELLS;

BIOCHEMICAL ANALYSIS; BIOMOLECULAR ANALYSIS; CELL ANALYSIS; CHIP-MS PLATFORM; ELECTRONIC METHODS; MAGNETIC OPERATION; MICROFLUIDIC CHIP; OPTICAL DETECTORS;

MICROFLUIDICS;

AMPICILLIN; BORTEZOMIB; CAPECITABINE; DOXYCYCLINE;

ACOUSTIC ANALYSIS; BIOCHEMICAL ANALYSIS; BIOCHIP; BIOMIMETICS; BIOSENSOR; DNA EXTRACTION; DRUG SCREENING; ELECTRONICS; FLUORESCENCE RESONANCE ENERGY TRANSFER; GENETIC ANALYSIS; HUMAN; HYDROGEL; INFRARED SPECTROSCOPY; LAB ON A CHIP; MAGNETISM; MASS SPECTROMETRY; MICROFLUIDIC CHIP; MICROFLUIDICS; NONHUMAN; OPTICAL INSTRUMENTATION; PRIORITY JOURNAL; PROTEIN ANALYSIS; REVIEW; SINGLE CELL ANALYSIS; STEM CELL; STEM CELL CULTURE;

EID: 84962239127     PISSN: 01659936     EISSN: 18793142     Source Type: Journal    
DOI: 10.1016/j.trac.2016.03.013     Document Type: Review

References (227)

1. El-Ali J., Sorger P.K., Jensen K.F. Cells on chips. Nature 2006, 442:403-411.
2. Rosenkilde M.M., Schwartz T.W. The chemokine system - a major regulator of angiogenesis in health and disease. APMIS 2004, 112:481-495.
3. Liu L., Ratner B.D., Sage E.H., Jiang S. Endothelial cell migration on surface-density gradients of fibronectin, VEGF, or both proteins. Langmuir 2007, 23:11168-11173.
4. Belloni A.S., Albertin G., Forneris M.L., Nussdorfer G.G. Proadrenomedullin-derived peptides as autocrine-paracrine regulators of cell growth. Histol. Histopathol 2001, 16:1263-1274.
5. Rosso F., Giordano A., Barbarisi M., Barbarisi A. From cell-ECM interactions to tissue engineering. J. Cell. Physiol 2004, 199:174-180.
6. Jamora C., Fuchs E. Intercellular adhesion, signalling and the cytoskeleton. Nat. Cell Biol 2002, 4:E101-E108.
7. Geiger B., Bershadsky A., Pankov R., Yamada K.M. Transmembrane extracellular matrix-cytoskeleton crosstalk. Nat. Rev. Mol. Cell Biol 2001, 2:793-805.
8. Li Y., Zhou Y., Wang H., Perrett S., Zhao Y., Tang Z., et al. Chirality of glutathione surface coating affects the cytotoxicity of quantum dots. Angew. Chem. Int. Edit 2011, 50:5860-5864.
9. Hoshino A., Fujioka K., Oku T., Suga M., Sasaki Y.F., Ohta T., et al. Physicochemical properties and cellular toxicity of nanocrystal quantum dots depend on their surface modification. Nano Lett 2004, 4:2163-2169.
10. Arora A., Simone G., Salieb-Beugelaar G.B., Kim J.T., Manz A. Latest developments in micro total analysis systems. Anal. Chem 2010, 82:4830-4847.
11. Salieb-Beugelaar G.B., Simone G., Arora A., Philippi A., Manz A. Latest developments in microfluidic cell biology and analysis systems. Anal. Chem 2010, 82:4848-4864.
12. Mark D., Haeberle S., Roth G., von Stetten F., Zengerle R. Microfluidic lab-on-a-chip platforms: requirements, characteristics and applications. Chem. Soc. Rev 2010, 39:1153-1182.
13. Meyvantsson I., Beebe D.J. Cell culture models in microfluidic systems. Annu. Rev. Anal. Chem. (Palo Alto Calif) 2008, 1:423-449.
14. Khademhosseini A., Langer R., Borenstein J., Vacanti J.P. Microscale technologies for tissue engineering and biology. Proc. Natl. Acad. Sci. U.S.A. 2006, 103:2480-2487.
15. Gao D., Liu H., Jiang Y., Lin J.-M. Recent developments in microfluidic devices for in vitro cell culture for cell-biology research. TrAC - Trends Anal. Chem 2012, 35:150-164.
16. Cushing M.C., Anseth K.S. Hydrogel cell cultures. Science 2007, 316:1133-1134.
17. Huh D., Hamilton G.A., Ingber D.E. From 3D cell culture to organs-on-chips. Trends Cell Biol 2011, 21:745-754.
18. Imura Y., Sato K., Yoshimura E. Micro total bioassay system for ingested substances: assessment of intestinal absorption, hepatic metabolism, and bioactivity. Anal. Chem 2010, 82:9983-9988.
19. Huh D., Matthews B.D., Mammoto A., Montoya-Zavala M., Hsin H.Y., Ingber D.E. Reconstituting organ-level lung functions on a chip. Science 2010, 328:1662-1668.
20. Mazutis L., Gilbert J., Ung W.L., Weitz D.A., Griffiths A.D., Heyman J.A. Single-cell analysis and sorting using droplet-based microfluidics. Nat. Protoc 2013, 8:870-891.
21. Gefen O., Fridman O., Ronin I., Balaban N.Q. Direct observation of single stationary-phase bacteria reveals a surprisingly long period of constant protein production activity. Proc. Natl. Acad. Sci. U.S.A. 2014, 111:556-561.
22. Lee S.S., Avalos Vizcarra I., Huberts D.H.E.W., Lee L.P., Heinemann M. Whole lifespan microscopic observation of budding yeast aging through a microfluidic dissection platform. Proc. Natl. Acad. Sci. U.S.A. 2012, 109:4916-4920.
23. Loutherback K., Chen L., Holman H.N. Open-channel microfluidic membrane device for long-term FT-IR spectromicroscopy of live adherent cells. Anal. Chem 2015, 87:4601-4606.
24. Seefeld T.H., Halpern A.R., Corn R.M. On-Chip synthesis of protein microarrays from DNA microarrays via coupled in vitro transcription and translation for surface plasmon resonance imaging biosensor applications. J. Am. Chem. Soc 2012, 134:12358-12361.
25. Bermejo C., Haerizadeh F., Takanaga H., Chermak D., Frommer W.B. Optical sensors for measuring dynamic changes of cytosolic metabolite levels in yeast. Nat. Protoc 2011, 6:1806-1817.
26. Xin H., Li Y., Li B. Controllable patterning of different cells via optical assembly of 1D periodic cell structures. Adv. Funct. Mater 2015, 25:2816-2823.
27. Kim S., Streets A.M., Lin R.R., Quake S.R., Weiss S., Majumdar D.S. High-throughput single-molecule optofluidic analysis. Nat. Methods 2011, 8:242-245.
28. Guo F., Lapsley M.I., Nawaz A.A., Zhao Y., Lin S.S., Chen Y., et al. A droplet-based, optofluidic device for high-throughput, quantitative bioanalysis. Anal. Chem 2012, 84:10745-10749.
29. Chen J., Kang Z., Wang G., Loo J.F.C., Kong S.K., Ho H. Optofluidic guiding, valving, switching and mixing based on plasmonic heating in a random gold nanoisland substrate. Lab Chip 2015, 15:2504-2512.
30. Valero A., Braschler T., Renaud P. A unified approach to dielectric single cell analysis: impedance and dielectrophoretic force spectroscopy. Lab Chip 2010, 10:2216-2225.
31. Tran T.B., Cho S., Min J. Hydrogel-based diffusion chip with Electric Cell-substrate Impedance Sensing (ECIS) integration for cell viability assay and drug toxicity screening. Biosens. Bioelectron 2013, 50:453-459.
32. Xu Y., Lv Y., Wang L., Xing W., Cheng J. A microfluidic device with passive air-bubble valves for real-time measurement of dose-dependent drug cytotoxicity through impedance sensing. Biosens. Bioelectron 2012, 32:300-304.
33. Zor K., Heiskanen A., Caviglia C., Vergani M., Landini E., Shah F., et al. A compact multifunctional microfluidic platform for exploring cellular dynamics in real-time using electrochemical detection. RSC Adv 2014, 4:63761-63771.
34. Meissner R., Eker B., Kasi H., Bertsch A., Renaud P. Distinguishing drug-induced minor morphological changes from major cellular damage via label-free impedimetric toxicity screening. Lab Chip 2011, 11:2352-2361.
35. Sabuncu A.C., Zhuang J., Kolb J.F., Beskok A. Microfluidic impedance spectroscopy as a tool for quantitative biology and biotechnology. Biomicrofluidics 2012, 6:34103.
36. Dworak B.J., Wheeler B.C. Novel MEA platform with PDMS microtunnels enables the detection of action potential propagation from isolated axons in culture. Lab Chip 2009, 9:404-410.
37. Hai A., Shappir J., Spira M.E. In-cell recordings by extracellular microelectrodes. Nat. Methods 2010, 7:200-202.
38. Cellot G., Cilia E., Cipollone S., Rancic V., Sucapane A., Giordani S., et al. Carbon nanotubes might improve neuronal performance by favouring electrical shortcuts. Nat. Nanotechnol 2009, 4:126-133.
39. Ng S.Y., Reboud J., Wang K.Y.P., Tang K.C., Zhang L., Wong P., et al. Label-free impedance detection of low levels of circulating endothelial progenitor cells for point-of-care diagnosis. Biosens. Bioelectron 2010, 25:1095-1101.
40. Du E., Ha S., Diez-Silva M., Dao M., Suresh S., Chandrakasan A.P. Electric impedance microflow cytometry for characterization of cell disease states. Lab Chip 2013, 13:3903-3909.
41. Nwankire C.E., Venkatanarayanan A., Glennon T., Keyes T.E., Forster R.J., Ducrée J. Label-free impedance detection of cancer cells from whole blood on an integrated centrifugal microfluidic platform. Biosens. Bioelectron 2015, 68:382-389.
42. Mannoor M.S., Zhang S., Link A.J., McAlpine M.C. Electrical detection of pathogenic bacteria via immobilized antimicrobial peptides. Proc. Natl. Acad. Sci. U.S.A. 2010, 107:19207-19212.
43. Gawad S., Cheung K., Seger U., Bertsch A., Renaud P. Dielectric spectroscopy in a micromachined flow cytometer: theoretical and practical considerations. Lab Chip 2004, 4:241-251.
44. Braschler T., Demierre N., Nascimento E., Silva T., Oliva A.G., Renaud P. Continuous separation of cells by balanced dielectrophoretic forces at multiple frequencies. Lab Chip 2008, 8:280-286.
45. Demierre N., Braschler T., Linderholm P., Seger U., van Lintel H., Renaud P. Characterization and optimization of liquid electrodes for lateral dielectrophoresis. Lab Chip 2007, 7:355-365.
46. Demierre N., Braschler T., Muller R., Renaud P. Focusing and continuous separation of cells in a microfluidic device using lateral dielectrophoresis. Sens. Actuator. B-Chem 2008, 132:388-396.
47. Zhang P., Ren L., Zhang X., Shan Y., Wang Y., Ji Y., et al. Raman-activated cell sorting based on dielectrophoretic single-cell trap and release. Anal. Chem 2015, 87:2282-2289.
48. Simon M.G., Li Y., Arulmoli J., McDonnell L.P., Akil A., Nourse J.L., et al. Increasing label-free stem cell sorting capacity to reach transplantation-scale throughput. Biomicrofluidics 2014, 8:64106.
49. Song H., Rosano J.M., Wang Y., Garson C.J., Prabhakarpandian B., Pant K., et al. Continuous-flow sorting of stem cells and differentiation products based on dielectrophoresis. Lab Chip 2015, 15:1320-1328.
50. Lee G., Wu H., Yang P., Mai J.D. Optically induced dielectropheresis sorting with automated medium exchange in an integrated optofluidic device resulting in higher cell viability. Lab Chip 2014, 14:2837-2843.
51. Wei Z., Li X., Zhao D., Yan H., Hu Z., Liang Z., et al. Flow-through cell electroporation microchip integrating dielectrophoretic viable cell sorting. Anal. Chem 2014, 86:10215-10222.
52. DuBose J., Lu X., Patel S., Qian S., Joo S.W., Xuan X. Microfluidic electrical sorting of particles based on shape in a spiral microchannel. Biomicrofluidics 2014, 8:14101.
53. Zhu J., Xuan X. Curvature-induced dielectrophoresis for continuous separation of particles by charge in spiral microchannels. Biomicrofluidics 2011, 5:24111.
54. Anand R.K., Johnson E.S., Chiu D.T. Negative dielectrophoretic capture and repulsion of single cells at a bipolar electrode: the impact of faradaic ion enrichment and depletion. J. Am. Chem. Soc 2015, 137:776-783.
55. Hughes A.J., Herr A.E. Microfluidic western blotting. Proc. Natl. Acad. Sci. U.S.A. 2012, 109:21450-21455.
56. Batalla P., Martin A., Angel Lopez M., Cristina Gonzalez M., Escarpa A. Enzyme-based microfluidic chip coupled to graphene electrodes for the detection of D-Amino acid enantiomer-biomarkers. Anal. Chem 2015, 87:5074-5078.
57. Kadimisetty K., Malla S., Sardesai N.P., Joshi A.A., Faria R.C., Lee N.H., et al. Automated multiplexed ECL immunoarrays for cancer biomarker proteins. Anal. Chem 2015, 87:4472-4478.
58. Vaidyanathan R., van Leeuwen L.M., Rauf S., Shiddiky M.J.A., Trau M. A multiplexed device based on tunable nanoshearing for specific detection of multiple protein biomarkers in serum. Sci. Rep 2015, 5:9756.
59. Wu L., Ma C., Ge L., Kong Q., Yan M., Ge S., et al. Paper-based electrochemiluminescence origami cyto-device for multiple cancer cells detection using porous AuPd alloy as catalytically promoted nanolabels. Biosens. Bioelectron 2015, 63:450-457.
60. Glavan A.C., Christodouleas D.C., Mosadegh B., Yu H.D., Smith B.S., Lessing J., et al. Folding analytical devices for electrochemical ELISA in hydrophobic R-H paper. Anal. Chem 2014, 86:11999-12007.
61. Ding X., Li P., Lin S.S., Stratton Z.S., Nama N., Guo F., et al. Surface acoustic wave microfluidics. Lab Chip 2013, 13:3626-3649.
62. Rogers P.R., Friend J.R., Yeo L.Y. Exploitation of surface acoustic waves to drive size-dependent microparticle concentration within a droplet. Lab Chip 2010, 10:2979-2985.
63. Franke T., Braunmuller S., Schmid L., Wixforth A., Weitz D.A. Surface acoustic wave actuated cell sorting (SAWACS). Lab Chip 2010, 10:789-794.
64. Schmid L., Franke T. SAW-controlled drop size for flow focusing. Lab Chip 2013, 13:1691-1694.
65. Reboud J., Bourquin Y., Wilson R., Pall G.S., Jiwaji M., Pitt A.R., et al. Shaping acoustic fields as a toolset for microfluidic manipulations in diagnostic technologies. Proc. Natl. Acad. Sci. U.S.A. 2012, 109:15162-15167.
66. Ding X., Lin S.S., Kiraly B., Yue H., Li S., Chiang I., et al. On-chip manipulation of single microparticles, cells, and organisms using surface acoustic waves. Proc. Natl. Acad. Sci. U.S.A. 2012, 109:11105-11109.
67. Li P., Mao Z., Peng Z., Zhou L., Chen Y., Huang P., et al. Acoustic separation of circulating tumor cells. Proc. Natl. Acad. Sci. U.S.A. 2015, 112:4970-4975.
68. Travagliati M., Shilton R.J., Pagliazzi M., Tonazzini I., Beltram F., Cecchini M. Acoustofluidics and whole-blood Manipulation in Surface Acoustic Wave Counterflow Devices. Anal. Chem 2014, 86:10633-10638.
69. Li S., Ding X., Mao Z., Chen Y., Nama N., Guo F., et al. Standing surface acoustic wave (SSAW)-based cell washing. Lab Chip 2015, 15:331-338.
70. Richter R.P., Bérat R., Brisson A.R. Formation of solid-supported Lipid Bilayers: an Integrated View. Langmuir 2006, 22:3497-3505.
71. Hennig M., Neumann J., Wixforth A., Radler J.O., Schneider M.F. Dynamic patterns in a supported lipid bilayer driven by standing surface acoustic waves. Lab Chip 2009, 9:3050-3053.
72. Hennig M., Wolff M., Neumann J., Wixforth A., Schneider M.F., Rädler J.O. DNA concentration modulation on supported lipid bilayers switched by surface acoustic waves. Langmuir 2011, 27:14721-14725.
73. Neumann J., Hennig M., Wixforth A., Manus S., Rädler J.O., Schneider M.F. Transport, separation, and accumulation of proteins on supported lipid bilayers. Nano Lett 2010, 10:2903-2908.
74. Ding X., Peng Z., Lin S.S., Geri M., Li S., Li P., et al. Cell separation using tilted-angle standing surface acoustic waves. Proc. Natl. Acad. Sci. U.S.A. 2014, 111:12992-12997.
75. Li S., Guo F., Chen Y., Ding X., Li P., Wang L., et al. Standing surface acoustic wave based cell coculture. Anal. Chem 2014, 86:9853-9859.
76. van Reenen A., de Jong A.M., den Toonder J.M.J., Prins M.W.J. Integrated lab-on-chip biosensing systems based on magnetic particle actuation - a comprehensive review. Lab Chip 2014, 14:1966-1986.
77. Vilfan M., Potocnik A., Kavcic B., Osterman N., Poberaj I., Vilfan A., et al. Self-assembled artificial cilia. Proc. Natl. Acad. Sci. U.S.A. 2010, 107:1844-1847.
78. Zhu G., Nguyen N. Rapid magnetofluidic mixing in a uniform magnetic field. Lab Chip 2012, 12:4772-4780.
79. Ranzoni A., Janssen X.J.A., Ovsyanko M., van IJzendoorn L.J., Prins M.W.J. Magnetically controlled rotation and torque of uniaxial microactuators for lab-on-a-chip applications. Lab Chip 2010, 10:179-188.
80. Karle M., Miwa J., Czilwik G., Auwarter V., Roth G., Zengerle R., et al. Continuous microfluidic DNA extraction using phase-transfer magnetophoresis. Lab Chip 2010, 10:3284-3290.
81. Lin G., Makarov D., Medina-Sanchez M., Guix M., Baraban L., Cuniberti G., et al. Magnetofluidic platform for multidimensional magnetic and optical barcoding of droplets. Lab Chip 2015, 15:216-224.
82. Park S.Y., Handa H., Sandhu A. Magneto-optical biosensing platform based on light scattering from self-assembled chains of functionalized rotating magnetic beads. Nano Lett 2010, 10:446-451.
83. den Dulk R.C., Schmidt K.A., Sabatte G., Liebana S., Prins M.W.J. Magneto-capillary valve for integrated purification and enrichment of nucleic acids and proteins. Lab Chip 2013, 13:106-118.
84. Strohmeier O., Emperle A., Roth G., Mark D., Zengerle R., von Stetten F. Centrifugal gas-phase transition magnetophoresis (GTM) - a generic method for automation of magnetic bead based assays on the centrifugal microfluidic platform and application to DNA purification. Lab Chip 2013, 13:146-155.
85. Cornaglia M., Trouillon R., Tekin H.C., Lehnert T., Gijs M.A.M. Magnetic particle-scanning for ultrasensitive immunodetection On-Chip. Anal. Chem 2014, 86:8213-8223.
86. Chiou C., Jin Shin D., Zhang Y., Wang T. Topography-assisted electromagnetic platform for blood-to-PCR in a droplet. Biosens. Bioelectron 2013, 50:91-99.
87. Chang C., Huang W., Jalal S.I., Chan B., Mahmood A., Shahda S., et al. Circulating tumor cell detection using a parallel flow micro-aperture chip system. Lab Chip 2015, 15:1677-1688.
88. van Reenen A., Gao Y., Bos A.H., de Jong A.M., Hulsen M.A., den Toonder J.M.J., et al. Accurate quantification of magnetic particle properties by intra-pair magnetophoresis for nanobiotechnology. Appl. Phys. Lett 2013, 103:43704.
89. Hernández F., Portolés T., Pitarch E., López F.J. Gas chromatography coupled to high-resolution time-of-flight mass spectrometry to analyze trace-level organic compounds in the environment, food safety and toxicology. TrAC - Trends Anal. Chem 2011, 30:388-400.
90. Wei G., Zeng E.Y. Gas chromatography-mass spectrometry and high-performance liquid chromatography-tandem mass spectrometry in quantifying fatty acids. TrAC - Trends Anal. Chem 2011, 30:1429-1436.
91. Cajka T., Fiehn O. Comprehensive analysis of lipids in biological systems by liquid chromatography-mass spectrometry. TrAC - Trends Anal. Chem 2014, 61:192-206.
92. Rainville P.D., Theodoridis G., Plumb R.S., Wilson I.D. Advances in liquid chromatography coupled to mass spectrometry for metabolic phenotyping. TrAC - Trends Anal. Chem 2014, 61:181-191.
93. Hirayama A., Wakayama M., Soga T. Metabolome analysis based on capillary electrophoresis-mass spectrometry. TrAC - Trends Anal. Chem 2014, 61:215-222.
94. Seger C., Sturm S., Stuppner H. Mass spectrometry and NMR spectroscopy: modern high-end detectors for high resolution separation techniques - state of the art in natural product HPLC-MS, HPLC-NMR, and CE-MS hyphenations. Nat. Prod. Rep 2013, 30:970-987.
95. Timerbaev A.R. Capillary electrophoresis coupled to mass spectrometry for biospeciation analysis: critical evaluation. TrAC - Trends Anal. Chem 2009, 28:416-425.
96. Xue Q.F., Foret F., Dunayevskiy Y.M., Zavracky P.M., McGruer N.E., Karger B.L. Multichannel microchip electrospray mass spectrometry. Anal. Chem 1997, 69:426-430.
97. Ramsey R.S., Ramsey J.M. Generating electrospray from microchip devices using electroosmotic pumping. Anal. Chem 1997, 69:1174-1178.
98. Feng X., Liu B., Li J., Liu X. Advances in coupling microfluidic chips to mass spectrometry. Mass Spectrom. Rev 2015, 34:535-557.
99. Wei H., Li H., Lin J.-M. Analysis of herbicides on a single C30 bead via a microfluidic device combined with electrospray ionization quadrupole time-of-flight mass spectrometer. J. Chromatogr. A 2009, 1216:9134-9142.
100. Wei H., Li H., Gao D., Lin J.-M. Multi-channel microfluidic devices combined with electrospray ionization quadrupole time-of-flight mass spectrometry applied to the monitoring of glutamate release from neuronal cells. Analyst 2010, 135:2043-2050.
101. Gao D., Wei H., Guo G.S., Lin J.-M. Microfluidic cell culture and metabolism detection with electrospray ionization quadrupole time-of-flight mass spectrometer. Anal. Chem 2010, 82:5679-5685.
102. Gao D., Li H., Wang N., Lin J.-M. Evaluation of the absorption of methotrexate on cells and its cytotoxicity assay by using an integrated microfluidic device coupled to a mass spectrometer. Anal. Chem 2012, 84:9230-9237.
103. Gao D., Liu H., Lin J.-M., Wang Y., Jiang Y. Characterization of drug permeability in Caco-2 monolayers by mass spectrometry on a membrane-based microfluidic device. Lab Chip 2013, 13:978-985.
104. Chen Q., Wu J., Zhang Y., Lin J.-M. Qualitative and quantitative analysis of tumor cell metabolism via stable isotope labeling assisted microfluidic chip electrospray ionization mass spectrometry. Anal. Chem 2012, 84:1695-1701.
105. Wei H., Li H., Mao S., Lin J.-M. Cell signaling analysis by mass spectrometry under coculture conditions on an integrated microfluidic device. Anal. Chem 2011, 83:9306-9313.
106. Mao S., Zhang J., Li H., Lin J.-M. Strategy for signaling molecule detection by using an integrated microfluidic device coupled with mass spectrometry to study Cell-to-Cell communication. Anal. Chem 2013, 85:868-876.
107. Liu W., Chen Q., Lin X., Lin J.-M. Online multi-channel microfluidic chip-mass spectrometry and its application for quantifying noncovalent protein-protein interactions. Analyst 2015, 140:1551-1554.
108. Redman E.A., Batz N.G., Mellors J.S., Ramsey J.M. Integrated microfluidic capillary electrophoresis-electrospray ionization devices with online MS detection for the separation and characterization of intact monoclonal antibody variants. Anal. Chem 2015, 87:2264-2272.
109. van den Brink F.T.G., Büter L., Odijk M., Olthuis W., Karst U., van den Berg A. Mass spectrometric detection of short-lived drug metabolites generated in an electrochemical microfluidic chip. Anal. Chem 2015, 87:1527-1535.
110. Wu Q., Gao D., Wei J., Jin F., Xie W., Jiang Y., et al. Development of a novel multi-layer microfluidic device towards characterization of drug metabolism and cytotoxicity for drug screening. Chem. Commun 2014, 50:2762-2764.
111. Kirby A.E., Lafrenière N.M., Seale B., Hendricks P.I., Cooks R.G., Wheeler A.R. Analysis on the go: quantitation of drugs of abuse in dried urine with digital microfluidics and miniature mass spectrometry. Anal. Chem 2014, 86:6121-6129.
112. Jin D., Zhu Y., Fang Q. Swan probe: a nanoliter-scale and high-throughput sampling interface for coupling electrospray ionization mass spectrometry with microfluidic droplet array and multiwell plate. Anal. Chem 2014, 86:10796-10803.
113. Arscott S. SU-8 as a material for lab-on-a-chip-based mass spectrometry. Lab Chip 2014, 14:3668-3689.
114. Gao D., Liu H., Jiang Y., Lin J.-M. Recent advances in microfluidics combined with mass spectrometry: technologies and applications. Lab Chip 2013, 13:3309-3322.
115. He X., Chen Q., Zhang Y., Lin J.-M. Recent advances in microchip-mass spectrometry for biological analysis. TrAC - Trends Anal. Chem 2014, 53:84-97.
116. Allazetta S., Kolb L., Zerbib S., Bardy J., Lutolf M.P. Cell-instructive microgels with Tailor-made physicochemical properties. Small 2015, 11:5647-5656.
117. Bichsel C.A., Gobaa S., Kobel S., Secondini C., Thalmann G.N., Cecchini M.G., et al. Diagnostic microchip to assay 3D colony-growth potential of captured circulating tumor cells. Lab Chip 2012, 12:2313-2316.
118. Allazetta S., Cosson S., Lutolf M.P. Programmable microfluidic patterning of protein gradients on hydrogels. Chem. Commun 2011, 47:191-193.
119. Cosson S., Allazetta S., Lutolf M.P. Patterning of cell-instructive hydrogels by hydrodynamic flow focusing. Lab Chip 2013, 13:2099-2105.
120. Cosson S., Kobel S.A., Lutolf M.P. Capturing complex protein gradients on biomimetic hydrogels for cell-based assays. Adv. Funct. Mater 2009, 19:3411-3419.
121. Mathes S.H., Ruffner H., Graf-Hausner U. The use of skin models in drug development. Adv. Drug Deliv. Rev 2014, 69-70:81-102.
122. Li Y., Wang S., Huang R., Huang Z., Hu B., Zheng W., et al. Evaluation of the effect of the structure of bacterial cellulose on full thickness skin wound repair on a microfluidic chip. Biomacromolecules 2015, 16:780-789.
123. Torisawa Y., Spina C.S., Mammoto T., Mammoto A., Weaver J.C., Tat T., et al. Bone marrow-on-a-chip replicates hematopoietic niche physiology in vitro. Nat. Methods 2014, 11:663-669.
124. Wu J., Chen Q., Liu W., Zhang Y., Lin J.-M. Cytotoxicity of quantum dots assay on a microfluidic 3D-culture device based on modeling diffusion process between blood vessels and tissues. Lab Chip 2012, 12:3474-3480.
125. Park J., Lee B.K., Jeong G.S., Hyun J.K., Lee C.J., Lee S. Three-dimensional brain-on-a-chip with an interstitial level of flow and its application as an in vitro model of Alzheimer's disease. Lab Chip 2015, 15:141-150.
126. Zheng Y., Chen J., Craven M., Choi N.W., Totorica S., Diaz-Santana A., et al. In vitro microvessels for the study of angiogenesis and thrombosis. Proc. Natl. Acad. Sci. U.S.A. 2012, 109:9342-9347.
127. Ebrahimkhani M.R., Neiman J.A.S., Raredon M.S.B., Hughes D.J., Griffith L.G. Bioreactor technologies to support liver function in vitro. Adv. Drug Deliv. Rev 2014, 69-70:132-157.
128. Mao S., Gao D., Liu W., Wei H., Lin J.-M. Imitation of drug metabolism in human liver and cytotoxicity assay using a microfluidic device coupled to mass spectrometric detection. Lab Chip 2012, 12:219-226.
129. Jeon J.S., Bersini S., Gilardi M., Dubini G., Charest J.L., Moretti M., et al. Human 3D vascularized organotypic microfluidic assays to study breast cancer cell extravasation. Proc. Natl. Acad. Sci. U.S.A. 2015, 112:214-219.
130. Li Y., Zhang S., Wang X., Zhang X., Oleinick A.I., Svir I., et al. Real-time monitoring of discrete synaptic release events and excitatory potentials within self-reconstructed neuromuscular junctions. Angew. Chem. Int. Edit 2015, 54:9313-9318.
131. Wu M., Huang S., Lee G. Microfluidic cell culture systems for drug research. Lab Chip 2010, 10:939-956.
132. Tsui J.H., Lee W., Pun S.H., Kim J., Kim D. Microfluidics-assisted in vitro drug screening and carrier production. Adv. Drug Deliv. Rev 2013, 65:1575-1588.
133. Miller O.J., El Harrak A., Mangeat T., Baret J., Frenz L., El Debs B., et al. High-resolution dose-response screening using droplet-based microfluidics. Proc. Natl. Acad. Sci. U.S.A. 2012, 109:378-383.
134. Zhang J., Wu J., Li H., Chen Q., Lin J.-M. An in vitro liver model on microfluidic device for analysis of capecitabine metabolite using mass spectrometer as detector. Biosens. Bioelectron 2015, 68:322-328.
135. Shallan A.I., Guijt R.M., Breadmore M.C. Electrokinetic size and mobility traps for on-site therapeutic drug monitoring. Angew. Chem. Int. Edit 2015, 54:7359-7362.
136. Wang H., Chen C., Xiang Z., Wang M., Lee C. A convection-driven long-range linear gradient generator with dynamic control. Lab Chip 2015, 15:1445-1450.
137. Kim C., Kasuya J., Jeon J., Chung S., Kamm R.D. A quantitative microfluidic angiogenesis screen for studying anti-angiogenic therapeutic drugs. Lab Chip 2015, 15:301-310.
138. Dereli-Korkut Z., Akaydin H.D., Ahmed A.H.R., Jiang X., Wang S. Three dimensional microfluidic cell arrays for ex vivo drug screening with mimicked vascular flow. Anal. Chem 2014, 86:2997-3004.
139. Wu J., Chen Q., Liu W., Lin J.-M. A simple and versatile microfluidic cell density gradient generator for quantum dot cytotoxicity assay. Lab Chip 2013, 13:1948-1954.
140. Esch E.W., Bahinski A., Huh D. Organs-on-chips at the frontiers of drug discovery. Nat. Rev. Drug Discov 2015, 14:248-260.
141. Neuži P., Giselbrecht S., Länge K., Huang T.J., Manz A. Revisiting lab-on-a-chip technology for drug discovery. Nat. Rev. Drug Discov 2012, 11:620-632.
142. Chan C.Y., Huang P., Guo F., Ding X., Kapur V., Mai J.D., et al. Accelerating drug discovery via organs-on-chips. Lab Chip 2013, 13:4697-4710.
143. Abaci H.E., Gledhill K., Guo Z., Christiano A.M., Shuler M.L. Pumpless microfluidic platform for drug testing on human skin equivalents. Lab Chip 2015, 15:882-888.
144. Au S.H., Chamberlain M.D., Mahesh S., Sefton M.V., Wheeler A.R. Hepatic organoids for microfluidic drug screening. Lab Chip 2014, 14:3290-3299.
145. Agarwal A., Goss J.A., Cho A., McCain M.L., Parker K.K. Microfluidic heart on a chip for higher throughput pharmacological studies. Lab Chip 2013, 13:3599-3608.
146. Maschmeyer I., Lorenz A.K., Schimek K., Hasenberg T., Ramme A.P., Hubner J., et al. A four-organ-chip for interconnected long-term co-culture of human intestine, liver, skin and kidney equivalents. Lab Chip 2015, 15:2688-2699.
147. Tanaka H., Yamamoto S., Nakamura A., Nakashoji Y., Okura N., Nakamoto N., et al. Hands-Off preparation of monodisperse emulsion droplets using a poly(dimethylsiloxane) microfluidic chip for droplet digital PCR. Anal. Chem 2015, 87:4134-4143.
148. Dalerba P., Kalisky T., Sahoo D., Rajendran P.S., Rothenberg M.E., Leyrat A.A., et al. Single-cell dissection of transcriptional heterogeneity in human colon tumors. Nat. Biotechnol 2011, 29:1120-1127.
149. Lin X., Wu J., Liu W., Li H., Wang Z., Lin J.-M. Detection of BCR-ABL using one step reverse transcriptase- polymerase chain reaction and microchip electrophoresis. Methods 2013, 64:250-254.
150. Lin X., Wu J., Li H., Wang Z., Lin J.-M. Determination of mini-short tandem repeat (miniSTR) loci by using the combination of polymerase chain reaction (PCR) and microchip electrophoresis. Talanta 2013, 114:131-137.
151. Reinholt S.J., Baeumner A.J. Microfluidic isolation of nucleic acids. Angew. Chem. Int. Edit 2014, 53:13988-14001.
152. Shi X., Chen C., Gao W., Chao S., Meldrum D.R. Parallel RNA extraction using magnetic beads and a droplet array. Lab Chip 2015, 15:1059-1065.
153. White A.K., VanInsberghe M., Petriv O.I., Hamidi M., Sikorski D., Marra M.A., et al. High-throughput microfluidic single-cell RT-qPCR. Proc. Natl. Acad. Sci. U.S.A. 2011, 108:13999-14004.
154. Shao H., Chung J., Lee K., Balaj L., Min C., Carter B.S., et al. Chip-based analysis of exosomal mRNA mediating drug resistance in glioblastoma. Nat. Commun 2015, 6:6999.
155. Ben-Yoav H., Dykstra P.H., Bentley W.E., Ghodssi R. A controlled microfluidic electrochemical lab-on-a-chip for label-free diffusion-restricted DNA hybridization analysis. Biosens. Bioelectron 2015, 64:579-585.
156. White S.P., Dorfman K.D., Frisbie C.D. Label-Free DNA sensing platform with Low-Voltage Electrolyte-Gated transistors. Anal. Chem 2015, 87:1861-1866.
157. Polinkovsky M.E., Gambin Y., Banerjee P.R., Erickstad M.J., Groisman A., Deniz A.A. Ultrafast cooling reveals microsecond-scale biomolecular dynamics. Nat. Commun 2014, 5:5737.
158. Zhao Y., Chen D., Yue H., Spiering M.M., Zhao C., Benkovic S.J., et al. Dark-field illumination on zero-mode waveguide/microfluidic hybrid chip reveals T4 replisomal protein interactions. Nano Lett 2014, 14:1952-1960.
159. Forget A.L., Dombrowski C.C., Amitani I., Kowalczykowski S.C. Exploring protein-DNA interactions in 3D using in situ construction, manipulation and visualization of individual DNA dumbbells with optical traps, microfluidics and fluorescence microscopy. Nat. Protoc 2013, 8:525-538.
160. Colin P., Kintses B., Gielen F., Miton C.M., Fischer G., Mohamed M.F., et al. Ultrahigh-throughput discovery of promiscuous enzymes by picodroplet functional metagenomics. Nat. Commun 2015, 6:10008.
161. Busch W., Moore B.T., Martsberger B., Mace D.L., Twigg R.W., Jung J., et al. A microfluidic device and computational platform for high-throughput live imaging of gene expression. Nat. Methods 2012, 9:1101-1106.
162. Cho M., Xiao Y., Nie J., Stewart R., Csordas A.T., Oh S.S., et al. Quantitative selection of DNA aptamers through microfluidic selection and high-throughput sequencing. Proc. Natl. Acad. Sci. U.S.A. 2010, 107:15373-15378.
163. Streets A.M., Zhang X., Cao C., Pang Y., Wu X., Xiong L., et al. Microfluidic single-cell whole-transcriptome sequencing. Proc. Natl. Acad. Sci. U.S.A. 2014, 111:7048-7053.
164. Jo M.C., Liu W., Gu L., Dang W., Qin L. High-throughput analysis of yeast replicative aging using a microfluidic system. Proc. Natl. Acad. Sci. U.S.A. 2015, 112:9364-9369.
165. Krivitsky V., Hsiung L., Lichtenstein A., Brudnik B., Kantaev R., Elnathan R., et al. Si nanowires forest-based on-chip biomolecular filtering, separation and preconcentration devices: nanowires do it all. Nano Lett 2012, 12:4748-4756.
166. Shastri A., McGregor L.M., Liu Y., Harris V., Nan H., Mujica M., et al. An aptamer-functionalized chemomechanically modulated biomolecule catch-and-release system. Nat. Chem 2015, 7:447-454.
167. Fischlechner M., Schaerli Y., Mohamed M.F., Patil S., Abell C., Hollfelder F. Evolution of enzyme catalysts caged in biomimetic gel-shell beads. Nat. Chem 2014, 6:791-796.
168. Romero P.A., Tran T.M., Abate A.R. Dissecting enzyme function with microfluidic-based deep mutational scanning. Proc. Natl. Acad. Sci. U.S.A. 2015, 112:7159-7164.
169. Otten M., Ott W., Jobst M.A., Milles L.F., Verdorfer T., Pippig D.A., et al. From genes to protein mechanics on a chip. Nat. Methods 2014, 11:1127-1130.
170. Lin X., Chen Q., Liu W., Zhang J., Wang S., Lin Z., et al. Oxygen-induced cell migration and on-line monitoring biomarkers modulation of cervical cancers on a microfluidic system. Sci. Rep 2015, 5:9643.
171. Lin X., Chen Q., Liu W., Li H., Lin J.-M. A portable microchip for ultrasensitive and high-throughput assay of thrombin by rolling circle amplification and hemin/G-quadruplex system. Biosens. Bioelectron 2014, 56:71-76.
172. Lin X., Chen Q., Liu W., Yi L., Li H., Wang Z., et al. Assay of multiplex proteins from cell metabolism based on tunable aptamer and microchip electrophoresis. Biosens. Bioelectron 2015, 63:105-111.
173. Mok J., Mindrinos M.N., Davis R.W., Javanmard M. Digital microfluidic assay for protein detection. Proc. Natl. Acad. Sci. U.S.A. 2014, 111:2110-2115.
174. Ihara M., Yoshikawa A., Wu Y., Takahashi H., Mawatari K., Shimura K., et al. Micro OS-ELISA: rapid noncompetitive detection of a small biomarker peptide by open-sandwich enzyme-linked immunosorbent assay (OS-ELISA) integrated into microfluidic device. Lab Chip 2010, 10:92-100.
175. Kai J., Puntambekar A., Santiago N., Lee S.H., Sehy D.W., Moore V., et al. A novel microfluidic microplate as the next generation assay platform for enzyme linked immunoassays (ELISA). Lab Chip 2012, 12:4257-4262.
176. Wang T., Zhang M., Dreher D.D., Zeng Y. Ultrasensitive microfluidic solid-phase ELISA using an actuatable microwell-patterned PDMS chip. Lab Chip 2013, 13:4190-4197.
177. Cheng C., Martinez A.W., Gong J., Mace C.R., Phillips S.T., Carrilho E., et al. Paper-based ELISA. Angew. Chem. Int. Edit 2010, 49:4771-4774.
178. Li Y., Xu F., Liu C., Xu Y., Feng X., Liu B. A novel microfluidic mixer based on dual-hydrodynamic focusing for interrogating the kinetics of DNA-protein interaction. Analyst 2013, 138:4475-4482.
179. Han C., Zhang Q., Ma R., Xie L., Qiu T., Wang L., et al. Integration of single oocyte trapping, in vitrofertilization and embryo culture in a microwell-structured microfluidic device. Lab Chip 2010, 10:2848-2854.
180. Park M.C., Hur J.Y., Cho H.S., Park S., Suh K.Y. High-throughput single-cell quantification using simple microwell-based cell docking and programmable time-course live-cell imaging. Lab Chip 2011, 11:79-86.
181. Chen N., Chen C., Chen M., Jang L., Wang M. Single-cell trapping and impedance measurement utilizing dielectrophoresis in a parallel-plate microfluidic device. Sens. Actuator. B-Chem 2014, 190:570-577.
182. Wlodkowic D., Faley S., Zagnoni M., Wikswo J.P., Cooper J.M. Microfluidic single-cell array cytometry for the analysis of tumor apoptosis. Anal. Chem 2009, 81:5517-5523.
183. Koster S., Angile F.E., Duan H., Agresti J.J., Wintner A., Schmitz C., et al. Drop-based microfluidic devices for encapsulation of single cells. Lab Chip 2008, 8:1110-1115.
184. Clausell-Tormos J., Lieber D., Baret J., El-Harrak A., Miller O.J., Frenz L., et al. Droplet-based microfluidic platforms for the encapsulation and screening of mammalian cells and multicellular organisms. Chem. Biol 2008, 15:427-437.
185. Kobel S., Valero A., Latt J., Renaud P., Lutolf M. Optimization of microfluidic single cell trapping for long-term on-chip culture. Lab Chip 2010, 10:857-863.
186. Chen H., Sun J., Wolvetang E., Cooper-White J. High-throughput, deterministic single cell trapping and long-term clonal cell culture in microfluidic devices. Lab Chip 2015, 15:1072-1083.
187. Liberale C., Cojoc G., Bragheri F., Minzioni P., Perozziello G., La Rocca R., et al. Integrated microfluidic device for single-cell trapping and spectroscopy. Sci. Rep 2013, 3:1258.
188. Wu L., Chen P., Dong Y., Feng X., Liu B. Encapsulation of single cells on a microfluidic device integrating droplet generation with fluorescence-activated droplet sorting. Biomed. Microdevices 2013, 15:553-560.
189. Liu C., Liu J., Gao D., Ding M., Lin J.-M. Fabrication of microwell arrays based on two-dimensional ordered polystyrene microspheres for high-throughput single-cell analysis. Anal. Chem 2010, 82:9418-9424.
190. Chen Q., Wu J., Zhang Y., Lin Z., Lin J.-M. Targeted isolation and analysis of single tumor cells with aptamer-encoded microwell array on microfluidic device. Lab Chip 2012, 12:5180-5185.
191. Sun J., Wang J., Chen P., Feng X., Du W., Liu B. A chemical signal generator for resolving temporal dynamics of single cells. Anal. Bioanal. Chem 2011, 400:2973-2981.
192. Wang Y., Tang X., Feng X., Liu C., Chen P., Chen D., et al. A microfluidic digital single-cell assay for the evaluation of anticancer drugs. Anal. Bioanal. Chem 2015, 407:1139-1148.
193. Li Y., Chen D., Zhang Y., Liu C., Chen P., Wang Y., et al. High-throughput single cell multidrug resistance analysis with multifunctional gradients-customizing microfluidic device. Sens. Actuator. B-Chem 2016, 225:563-571.
194. Macosko E.Z., Basu A., Satija R., Nemesh J., Shekhar K., Goldman M., et al. Highly parallel genome-wide expression profiling of individual cells using nanoliter droplets. Cell 2015, 161:1202-1214.
195. Klein A.M., Mazutis L., Akartuna I., Tallapragada N., Veres A., Li V., et al. Droplet barcoding for single-cell transcriptomics applied to embryonic stem cells. Cell 2015, 161:1187-1201.
196. Li Y., Feng X., Du W., Li Y., Liu B. Ultrahigh-throughput approach for analyzing single-cell genomic damage with an agarose-based microfluidic comet array. Anal. Chem 2013, 85:4066-4073.
197. Nishikawa Y., Hosokawa M., Maruyama T., Yamagishi K., Mori T., Takeyama H. Monodisperse picoliter droplets for Low-Bias and Contamination-Free reactions in Single-Cell whole genome amplification. PLoS ONE 2015, 10:e138733.
198. Skelley A.M., Kirak O., Suh H., Jaenisch R., Voldman J. Microfluidic control of cell pairing and fusion. Nat. Methods 2009, 6:147-152.
199. Dura B., Dougan S.K., Barisa M., Hoehl M.M., Lo C.T., Ploegh H.L., et al. Profiling lymphocyte interactions at the single-cell level by microfluidic cell pairing. Nat. Commun 2015, 6:5940.
200. Boneschansker L., Yan J., Wong E., Briscoe D.M., Irimia D. Microfluidic platform for the quantitative analysis of leukocyte migration signatures. Nat. Commun 2014, 5:4787.
201. Lecault V., VanInsberghe M., Sekulovic S., Knapp D.J.H.F., Wohrer S., Bowden W., et al. High-throughput analysis of single hematopoietic stem cell proliferation in microfluidic cell culture arrays. Nat. Methods 2011, 8:581-586.
202. Chen Q., Wu J., Zhuang Q., Lin X., Zhang J., Lin J.-M. Microfluidic isolation of highly pure embryonic stem cells using feeder-separated co-culture system. Sci. Rep 2013, 3:2433.
203. Wu J., Li H., Chen Q., Lin X., Liu W., Lin J.-M. Statistical single-cell analysis of cell cycle-dependent quantum dot cytotoxicity and cellular uptake using a microfluidic system. RSC Adv 2014, 4:24929-24934.
204. Kellogg R.A., Gómez-Sjöberg R., Leyrat A.A., Tay S. High-throughput microfluidic single-cell analysis pipeline for studies of signaling dynamics. Nat. Protoc 2014, 9:1713-1726.
205. Shi Q., Qin L., Wei W., Geng F., Fan R., Shin Y.S., et al. Single-cell proteomic chip for profiling intracellular signaling pathways in single tumor cells. Proc. Natl. Acad. Sci. U.S.A. 2012, 109:419-424.
206. Hughes A.J., Spelke D.P., Xu Z., Kang C., Schaffer D.V., Herr A.E. Single-cell western blotting. Nat. Methods 2014, 11:749-755.
207. Xu F., Zhao H., Feng X., Chen L., Chen D., Zhang Y., et al. Single-cell chemical proteomics with an activity-based probe: identification of low-copy membrane proteins on primary neurons. Angew. Chem. Int. Edit 2014, 53:6730-6733.
208. Mahshid S., Ahamed M.J., Berard D., Amin S., Sladek R., Leslie S.R., et al. Development of a platform for single cell genomics using convex lens-induced confinement. Lab Chip 2015, 15:3013-3020.
209. Choi Y., Ingram P.N., Yang K., Coffman L., Iyengar M., Bai S., et al. Identifying an ovarian cancer cell hierarchy regulated by bone morphogenetic protein 2. Proc. Natl. Acad. Sci. U.S.A. 2015, 112:E6882-E6888.
210. Ertl P., Sticker D., Charwat V., Kasper C., Lepperdinger G. Lab-on-a-chip technologies for stem cell analysis. Trends Biotechnol 2014, 32:245-253.
211. Occhetta P., Centola M., Tonnarelli B., Redaelli A., Martin I., Rasponi M. High-throughput microfluidic platform for 3D cultures of mesenchymal stem cells, towards engineering developmental processes. Sci. Rep 2015, 5:10288.
212. Cambier T., Honegger T., Vanneaux V., Berthier J., Peyrade D., Blanchoin L., et al. Design of a 2D no-flow chamber to monitor hematopoietic stem cells. Lab Chip 2015, 15:77-85.
213. Morrison S.J., Spradling A.C. Stem cells and niches: mechanisms that promote stem cell maintenance throughout life. Cell 2008, 132:598-611.
214. Kobel S., Lutolf M.P. High-throughput methods to define complex stem cell niches. Biotechniques 2010, 48:IX-XXII.
215. Allazetta S., Hausherr T.C., Lutolf M.P. Microfluidic synthesis of cell-type-specific artificial extracellular matrix hydrogels. Biomacromolecules 2013, 14:1122-1131.
216. Gobaa S., Hoehnel S., Roccio M., Negro A., Kobel S., Lutolf M.P. Artificial niche microarrays for probing single stem cell fate in high throughput. Nat. Methods 2011, 8:949-955.
217. Roch A., Trachsel V., Lutolf M.P. Brief Report: single-cell analysis reveals cell division-independent emergence of megakaryocytes from phenotypic hematopoietic stem cells. Stem Cells 2015, 33:3152-3157.
218. Allazetta S., Lutolf M.P. Stem cell niche engineering through droplet microfluidics. Curr. Opin. Biotech 2015, 35:86-93.
219. Xu H., Heilshorn S.C. Microfluidic investigation of BDNF-enhanced neural stem cell chemotaxis in CXCL12 gradients. Small 2013, 9:585-595.
220. Shi X., Chen S., Zhou J., Yu H., Li L., Wu H. Directing osteogenesis of stem cells with drug-laden, polymer-microsphere-based micropatterns generated by teflon microfluidic chips. Adv. Funct. Mater 2012, 22:3799-3807.
221. Przybyla L.M., Voldman J. Attenuation of extrinsic signaling reveals the importance of matrix remodeling on maintenance of embryonic stem cell self-renewal. Proc. Natl. Acad. Sci. U.S.A. 2012, 109:835-840.
222. Kang W., Giraldo-Vela J.P., Nathamgari S.S.P., McGuire T., McNaughton R.L., Kessler J.A., et al. Microfluidic device for stem cell differentiation and localized electroporation of postmitotic neurons. Lab Chip 2014, 14:4486-4495.
223. Reitinger S., Wissenwasser J., Kapferer W., Heer R., Lepperdinger G. Electric impedance sensing in cell-substrates for rapid and selective multipotential differentiation capacity monitoring of human mesenchymal stem cells. Biosens. Bioelectron 2012, 34:63-69.
224. Kuo Y., Ho J.H., Yen T., Chen H., Lee O.K. Development of a surface plasmon resonance biosensor for real-time detection of osteogenic differentiation in live mesenchymal stem cells. PLoS ONE 2011, 6:e22382.
225. Ranga A., Gjorevski N., Lutolf M.P. Drug discovery through stem cell-based organoid models. Adv. Drug Deliv. Rev 2014, 69-70:19-28.
226. Kobel S., Lutolf M.P. Biomaterials meet microfluidics: building the next generation of artificial niches. Curr. Opin. Biotech 2011, 22:690-697.
227. Gjorevski N., Ranga A., Lutolf M.P. Bioengineering approaches to guide stem cell-based organogenesis. Development 2014, 141:1794-1804.