High-throughput analysis of single hematopoietic stem cell proliferation in microfluidic cell culture arrays

Nature Methods

Volumn 8, Issue 7, 2011, Pages 581-589

  Lecault, Véronique ^a,b   Vaninsberghe, Michael ^b   Sekulovic, Sanja ^c   Knapp, David J H F ^c   Wohrer, Stefan ^c   Bowden, William ^a,b   Viel, Francis ^b   McLaughlin, Thomas ^b   Jarandehei, Asefeh ^a,b   Miller, Michelle ^c   Falconnet, Didier ^b   White, Adam K ^b   Kent, David G ^c   Copley, Michael R ^c   Taghipour, Fariborz ^b   Eaves, Connie J ^b,c   Humphries, R Keith ^b,c   Piret, James M ^a,b   Hansen, Carl L ^b  

^a UNIVERSITY OF BRITISH COLUMBIA   (Canada)

^b UNIVERSITY OF BRITISH COLUMBIA   (Canada)

^c BRITISH COLUMBIA CANCER AGENCY   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

STEEL FACTOR;

ANIMAL CELL; ARTICLE; CELL CULTURE; CELL PROLIFERATION; CONTROLLED STUDY; HEMATOPOIETIC STEM CELL; IMMUNOHISTOCHEMISTRY; IN VITRO STUDY; IN VIVO STUDY; MICROFLUIDIC ANALYSIS; MOUSE; NONHUMAN; PRIORITY JOURNAL;

ADULT; CELL CULTURE TECHNIQUES; CELL PROLIFERATION; HEMATOPOIETIC STEM CELLS; HIGH-THROUGHPUT SCREENING ASSAYS; HUMANS; MICROFLUIDIC ANALYTICAL TECHNIQUES; TISSUE ARRAY ANALYSIS;

MAMMALIA;

EID: 79959830458     PISSN: 15487091     EISSN: 15491676     Source Type: Journal    
DOI: 10.1038/nmeth.1614     Document Type: Article

References (30)

1. Kent, D.G. et al. Prospective isolation and molecular characterization of hematopoietic stem cells with durable self-renewal potential. Blood 113, 6342-6350 (2009).
2. Dykstra, B. et al. Long-term propagation of distinct hematopoietic differentiation programs in vivo. Cell Stem Cell 1, 218-229 (2007). (Pubitemid 47215588)
3. Yamazaki, S. & Nakauchi, H. Insights into signaling and function of hematopoietic stem cells at the single-cell level. Curr. Opin. Hematol. 16, 255-258 (2009).
4. Dykstra, B. et al. High-resolution video monitoring of hematopoietic stem cells cultured in single-cell arrays identifies new features of self-renewal. Proc. Natl. Acad. Sci. USA 103, 8185-8190 (2006). (Pubitemid 43801073)
5. Kent, D.G., Dykstra, B.J., Cheyne, J., Ma, E. & Eaves, C.J. Steel factor coordinately regulates the molecular signature and biologic function of hematopoietic stem cells. Blood 112, 560-567 (2008).
6. Brummendorf, T.H., Dragowska, W., Zijlmans, J., Thornbury, G. & Lansdorp, P.M. Asymmetric cell divisions sustain long-term hematopoiesis from single-sorted human fetal liver cells. J. Exp. Med. 188, 1117-1124 (1998). (Pubitemid 28443335)
7. Audet, J., Miller, C.L., Eaves, C.J. & Piret, J.M. Common and distinct features of cytokine effects on hematopoietic stem and progenitor cells revealed by dose-response surface analysis. Biotechnol. Bioeng. 80, 393-404 (2002). (Pubitemid 35230933)
8. Schroeder, T. Asymmetric cell division in normal and malignant hematopoietic precursor cells. Cell Stem Cell 1, 479-481 (2007). (Pubitemid 350063726)
9. Lutolf, M.P., Doyonnas, R., Havenstrite, K., Koleckar, K. & Blau, H.M. Perturbation of single hematopoietic stem cell fates in artificial niches. Integr. Biol. 1, 59-69 (2009).
10. Balagadde, F.K., You, L.C., Hansen, C.L., Arnold, F.H. & Quake, S.R. Long-term monitoring of bacteria undergoing programmed population control in a microchemostat. Science 309, 137-140 (2005). (Pubitemid 40934993)
11. Taylor, R.J. et al. Dynamic analysis of MAPK signaling using a high-throughput microfluidic single-cell imaging platform. Proc. Natl. Acad. Sci. USA 106, 3758-3763 (2009).
12. El-Ali, J., Sorger, P.K. & Jensen, K.F. Cells on chips. Nature 442, 403-411 (2006). (Pubitemid 44264784)
13. Faley, S.L. et al. Microfluidic single cell arrays to interrogate signalling dynamics of individual, patient-derived hematopoietic stem cells. Lab Chip 9, 2659-2664 (2009).
14. Wang, Z.H., Kim, M.C., Marquez, M. & Thorsen, T. High-density microfluidic arrays for cell cytotoxicity analysis. Lab Chip 7, 740-745 (2007). (Pubitemid 46848696)
15. Figallo, E. et al. Micro-bioreactor array for controlling cellular microenvironments. Lab Chip 7, 710-719 (2007). (Pubitemid 46850685)
16. Kim, L., Vahey, M.D., Lee, H.Y. & Voldman, J. Microfluidic arrays for logarithmically perfused embryonic stem cell culture. Lab Chip 6, 394-406 (2006). (Pubitemid 43327565)
17. Korin, N., Bransky, A., Dinnar, U. & Levenberg, S. Periodic "flow-stop" perfusion microchannel bioreactors for mammalian and human embryonic stem cell long-term culture. Biomed. Microdevices 11, 87-94 (2009).
18. Lee, P.J., Hung, P.J., Rao, V.M. & Lee, L.P. Nanoliter scale microbioreactor array for quantitative cell biology. Biotechnol. Bioeng. 94, 5-14 (2006). (Pubitemid 43723194)
19. Paguirigan, A.L. & Beebe, D.J. From the cellular perspective: exploring differences in the cellular baseline in macroscale and microfluidic cultures. Integr. Biol. 1, 182-195 (2009).
20. Unger, M.A., Chou, H.P., Thorsen, T., Scherer, A. & Quake, S.R. Monolithic microfabricated valves and pumps by multilayer soft lithography. Science 288, 113-116 (2000). (Pubitemid 30203039)
21. Thorsen, T., Maerkl, S.J. & Quake, S.R. Microfluidic large-scale integration. Science 298, 580-584 (2002). (Pubitemid 35215308)
22. Berthier, E., Warrick, J., Yu, H. & Beebe, D.J. Managing evaporation for more robust microscale assays. Part 1. Volume loss in high throughput assays. Lab Chip 8, 852-859 (2008). (Pubitemid 351746707)
23. Regehr, K.J. et al. Biological implications of polydimethylsiloxane-based microfluidic cell culture. Lab Chip 9, 2132-2139 (2009).
24. Heo, Y.S. et al. Characterization and resolution of evaporation-mediated osmolality shifts that constrain microfluidic cell culture in poly(dimethylsiloxane) devices. Anal. Chem. 79, 1126-1134 (2007).
25. Hansen, C.L., Classen, S., Berger, J.M. & Quake, S.R. A microfluidic device for kinetic optimization of protein crystallization and in situ structure determination. J. Am. Chem. Soc. 128, 3142-3143 (2006).
26. Ma, N.N., Koelling, K.W. & Chalmers, J.J. Fabrication and use of a transient contractional flow device to quantify the sensitivity of mammalian and insect cells to hydrodynamic forces. Biotechnol. Bioeng. 80, 428-437 (2002). (Pubitemid 35230937)
27. Pineault, N., Abramovich, C. & Humphries, R.K. Transplantable cell lines generated with NUP98-Hox fusion genes undergo leukemic progression by Meis1 independent of its binding to DNA. Leukemia 19, 636-643 (2005). (Pubitemid 40521169)
28. Ohta, H. et al. Near-maximal expansions of hematopoietic stem cells in culture using NUP98-HOX fusions. Exp. Hematol. 35, 817-830 (2007). (Pubitemid 46601757)
29. Bowie, M.B., Kent, D.G., Copley, M.R. & Eaves, C.J. Steel factor responsiveness regulates the high self-renewal phenotype of fetal hematopoietic stem cells. Blood 109, 5043-5048 (2007). (Pubitemid 46827804)
30. Satyanarayana, S., Karnik, R.N. & Majumdar, A. Stamp-and-stick room-temperature bonding technique for microdevices. J. Microelectromech. Syst. 14, 392-399 (2005). (Pubitemid 40679629)