메뉴 건너뛰기




Volumn 8, Issue 5, 2013, Pages 870-891

Single-cell analysis and sorting using droplet-based microfluidics

Author keywords

[No Author keywords available]

Indexed keywords

CELL SURFACE PROTEIN;

EID: 84877057770     PISSN: 17542189     EISSN: 17502799     Source Type: Journal    
DOI: 10.1038/nprot.2013.046     Document Type: Article
Times cited : (1077)

References (95)
  • 1
    • 84862212456 scopus 로고    scopus 로고
    • Droplet microfluidics for high-throughput biological assays
    • Guo, M.T., Rotem, A., Heyman, J.A. & Weitz, D.A. Droplet microfluidics for high-throughput biological assays. Lab Chip 12, 2146-2155 (2012
    • (2012) Lab Chip , vol.12 , pp. 2146-2155
    • Guo, M.T.1    Rotem, A.2    Heyman, J.A.3    Weitz, D.A.4
  • 3
    • 77955563148 scopus 로고    scopus 로고
    • Microdroplets in microfluidics: An evolving platform for discoveries in chemistry and biology
    • Theberge, A.B. et al. Microdroplets in microfluidics: An evolving platform for discoveries in chemistry and biology. Angew. Chem. Int. Ed. 49, 5846-5868 (2010
    • (2010) Angew. Chem. Int. Ed. , vol.49 , pp. 5846-5868
    • Theberge, A.B.1
  • 4
    • 0032832775 scopus 로고    scopus 로고
    • Drug screening-beyond the bottleneck
    • Dove, A. Drug screening-beyond the bottleneck. Nat. Biotechnol. 17, 859-863 (1999
    • (1999) Nat. Biotechnol , vol.17 , pp. 859-863
    • Dove, A.1
  • 5
    • 1642351216 scopus 로고    scopus 로고
    • Geometrically mediated breakup of drops in microfluidic devices
    • Link, D.R., Anna, S.L., Weitz, D.A. & Stone, H.A. Geometrically mediated breakup of drops in microfluidic devices. Phys. Rev. Lett. 92, 054503 (2004
    • (2004) Phys. Rev. Lett , vol.92 , pp. 054503
    • Link, D.R.1    Anna, S.L.2    Weitz, D.A.3    Stone, H.A.4
  • 6
    • 69549097381 scopus 로고    scopus 로고
    • A fast and efficient microfluidic system for highly selective one-to-one droplet fusion
    • Mazutis, L., Baret, J.C. & Griffiths, A.D. A fast and efficient microfluidic system for highly selective one-to-one droplet fusion. Lab Chip 9, 2665-2672 (2009
    • (2009) Lab Chip , vol.9 , pp. 2665-2672
    • Mazutis, L.1    Baret, J.C.2    Griffiths, A.D.3
  • 7
    • 84860353468 scopus 로고    scopus 로고
    • Selective droplet coalescence using microfluidic systems
    • Mazutis, L. & Griffiths, A.D. Selective droplet coalescence using microfluidic systems. Lab Chip 12, 1800-1806 (2012
    • (2012) Lab Chip , vol.12 , pp. 1800-1806
    • Mazutis, L.1    Griffiths, A.D.2
  • 8
    • 33745613020 scopus 로고    scopus 로고
    • Electrocoalescence of drops synchronized by size-dependent flow in microfluidic channels
    • Ahn, K., Agresti, J., Chong, H., Marquez, M. & Weitz, D.A. Electrocoalescence of drops synchronized by size-dependent flow in microfluidic channels. Appl. Phys. Lett. 88, 264105-264103 (2006
    • (2006) Appl. Phys. Lett , vol.88 , pp. 264105-264103
    • Ahn, K.1    Agresti, J.2    Chong, H.3    Marquez, M.4    Weitz, D.A.5
  • 9
    • 27244443193 scopus 로고    scopus 로고
    • Droplet fusion by alternating current (AC) field electrocoalescence in microchannels
    • Chabert, M., Dorfman, K.D. & Viovy, J.L. Droplet fusion by alternating current (AC) field electrocoalescence in microchannels. Electrophoresis 26, 3706-3715 (2005
    • (2005) Electrophoresis , vol.26 , pp. 3706-3715
    • Chabert, M.1    Dorfman, K.D.2    Viovy, J.L.3
  • 10
    • 33749239595 scopus 로고    scopus 로고
    • Controlled electrocoalescence in microfluidics: Targeting a single lamella
    • Priest, C., Herminghaus, S. & Seemann, R. Controlled electrocoalescence in microfluidics: Targeting a single lamella. Appl. Phys. Lett. 89, 134101 (2006
    • (2006) Appl. Phys. Lett , vol.89 , pp. 134101
    • Priest, C.1    Herminghaus, S.2    Seemann, R.3
  • 12
    • 33845864473 scopus 로고    scopus 로고
    • Nanoliter microfluidic hybrid method for simultaneous screening and optimization validated with crystallization of membrane proteins
    • Li, L. et al. Nanoliter microfluidic hybrid method for simultaneous screening and optimization validated with crystallization of membrane proteins. Proc. Natl. Acad. Sci. USA 103, 19243-19248 (2006
    • (2006) Proc. Natl. Acad. Sci. USA , vol.103 , pp. 19243-19248
    • Li, L.1
  • 13
    • 54349106182 scopus 로고    scopus 로고
    • Pillar-induced droplet merging in microfluidic circuits
    • Niu, X., Gulati, S., Edel, J.B. & deMello, A.J. Pillar-induced droplet merging in microfluidic circuits. Lab Chip 8, 1837-1841 (2008
    • (2008) Lab Chip , vol.8 , pp. 1837-1841
    • Niu, X.1    Gulati, S.2    Edel, J.B.3    DeMello, A.J.4
  • 14
    • 65649102240 scopus 로고    scopus 로고
    • Reliable microfluidic on-chip incubation of droplets in delay-lines
    • Frenz, L., Blank, K., Brouzes, E. & Griffiths, A.D. Reliable microfluidic on-chip incubation of droplets in delay-lines. Lab Chip 9, 1344-1348 (2009
    • (2009) Lab Chip , vol.9 , pp. 1344-1348
    • Frenz, L.1    Blank, K.2    Brouzes, E.3    Griffiths, A.D.4
  • 15
    • 80054988291 scopus 로고    scopus 로고
    • 1-Million droplet array with wide-field fluorescence imaging for digital PCR
    • Hatch, A.C. et al. 1-Million droplet array with wide-field fluorescence imaging for digital PCR. Lab Chip 11, 3838-3845 (2011
    • (2011) Lab Chip , vol.11 , pp. 3838-3845
    • Hatch, A.C.1
  • 16
    • 70350331103 scopus 로고    scopus 로고
    • Simultaneous determination of gene expression and enzymatic activity in individual bacterial cells in microdroplet compartments
    • Shim, J.U. et al. Simultaneous determination of gene expression and enzymatic activity in individual bacterial cells in microdroplet compartments. J. Am. Chem. Soc. 42, 15251-15256 (2009
    • (2009) J. Am. Chem. Soc , vol.42 , pp. 15251-15256
    • Shim, J.U.1
  • 17
    • 67249135232 scopus 로고    scopus 로고
    • Droplet-based microfluidic systems for high-throughput single DNA molecule isothermal amplification and analysis
    • Mazutis, L. et al. Droplet-based microfluidic systems for high-throughput single DNA molecule isothermal amplification and analysis. Anal. Chem. 81, 4813-4821 (2009
    • (2009) Anal. Chem , vol.81 , pp. 4813-4821
    • Mazutis, L.1
  • 19
    • 84856748588 scopus 로고    scopus 로고
    • Teaching single-cell digital analysis using droplet-based microfluidics
    • Najah, M., Griffiths, A.D. & Ryckelynck, M. Teaching single-cell digital analysis using droplet-based microfluidics. Anal. Chem. 84, 1202-1209 (2012
    • (2012) Anal. Chem , vol.84 , pp. 1202-1209
    • Najah, M.1    Griffiths, A.D.2    Ryckelynck, M.3
  • 20
    • 30744474931 scopus 로고    scopus 로고
    • Dielectrophoretic manipulation of drops for high-speed microfluidic sorting devices
    • Ahn, K. et al. Dielectrophoretic manipulation of drops for high-speed microfluidic sorting devices. Appl. Phys. Lett. 88, 024104 (2006
    • (2006) Appl. Phys. Lett , vol.88 , pp. 024104
    • Ahn, K.1
  • 21
    • 69549119766 scopus 로고    scopus 로고
    • Surface acoustic wave (SAW) directed droplet flow in microfluidics for PDMS devices
    • Franke, T., Abate, A.R., Weitz, D.A. & Wixforth, A. Surface acoustic wave (SAW) directed droplet flow in microfluidics for PDMS devices. Lab Chip 9, 2625-2627 (2009
    • (2009) Lab Chip , vol.9 , pp. 2625-2627
    • Franke, T.1    Abate, A.R.2    Weitz, D.A.3    Wixforth, A.4
  • 23
    • 77949552684 scopus 로고    scopus 로고
    • High-throughput screening of enzymes by retroviral display using droplet-based microfluidics
    • Granieri, L., Baret, J.C., Griffiths, A.D. & Merten, C.A. High-throughput screening of enzymes by retroviral display using droplet-based microfluidics. Chem. Biol. 17, 229-235 (2010
    • (2010) Chem. Biol , vol.17 , pp. 229-235
    • Granieri, L.1    Baret, J.C.2    Griffiths, A.D.3    Merten, C.A.4
  • 24
    • 15444376608 scopus 로고    scopus 로고
    • Selective encapsulation of single cells and subcellular organelles into picoliter- and femtoliter-volume droplets
    • He, M. et al. Selective encapsulation of single cells and subcellular organelles into picoliter- and femtoliter-volume droplets. Anal. Chem. 77, 1539-1544 (2005
    • (2005) Anal. Chem , vol.77 , pp. 1539-1544
    • He, M.1
  • 25
    • 43149111583 scopus 로고    scopus 로고
    • Droplet-based microfluidic platforms for the encapsulation and screening of mammalian cells and multicellular organisms
    • Clausell-Tormos, J. et al. Droplet-based microfluidic platforms for the encapsulation and screening of mammalian cells and multicellular organisms. Chem. Biol. 15, 427-437 (2008
    • (2008) Chem. Biol , vol.15 , pp. 427-437
    • Clausell-Tormos, J.1
  • 26
    • 46149091744 scopus 로고    scopus 로고
    • Drop-based microfluidic devices for encapsulation of single cells
    • Koster, S. et al. Drop-based microfluidic devices for encapsulation of single cells. Lab Chip 8, 1110-1115 (2008
    • (2008) Lab Chip , vol.8 , pp. 1110-1115
    • Koster, S.1
  • 27
    • 70149096883 scopus 로고    scopus 로고
    • Droplet microfluidic technology for single-cell high-throughput screening
    • Brouzes, E. et al. Droplet microfluidic technology for single-cell high-throughput screening. Proc. Natl. Acad. Sci. USA 106, 14195-14200 (2009
    • (2009) Proc. Natl. Acad. Sci. USA , vol.106 , pp. 14195-14200
    • Brouzes, E.1
  • 28
    • 69549121922 scopus 로고    scopus 로고
    • Isolation incubation, and parallel functional testing and identification by FISH of rare microbial single-copy cells from multi-species mixtures using the combination of chemistrode and stochastic confinement
    • Liu, W., Kim, H.J., Lucchetta, E.M., Du, W. & Ismagilov, R.F. Isolation, incubation, and parallel functional testing and identification by FISH of rare microbial single-copy cells from multi-species mixtures using the combination of chemistrode and stochastic confinement. Lab Chip 9, 2153-2162 (2009
    • (2009) Lab Chip , vol.9 , pp. 2153-2162
    • Liu, W.1    Kim, H.J.2    Lucchetta, E.M.3    Du, W.4    Ismagilov, R.F.5
  • 29
    • 66149131750 scopus 로고    scopus 로고
    • An integrated cell culture lab on a chip: Modular microdevices for cultivation of mammalian cells and delivery into microfluidic microdroplets
    • Hufnagel, H. et al. An integrated cell culture lab on a chip: Modular microdevices for cultivation of mammalian cells and delivery into microfluidic microdroplets. Lab Chip 9, 1576-1582 (2009
    • (2009) Lab Chip , vol.9 , pp. 1576-1582
    • Hufnagel, H.1
  • 30
    • 77951080101 scopus 로고    scopus 로고
    • High-performance single cell genetic analysis using microfluidic emulsion generator arrays
    • Zeng, Y., Novak, R., Shuga, J., Smith, M.T. & Mathies, R.A. High-performance single cell genetic analysis using microfluidic emulsion generator arrays. Anal. Chem. 82, 3183-3190 (2010
    • (2010) Anal. Chem , vol.82 , pp. 3183-3190
    • Zeng, Y.1    Novak, R.2    Shuga, J.3    Smith, M.T.4    Mathies, R.A.5
  • 31
    • 84865213657 scopus 로고    scopus 로고
    • Droplet microfluidics for amplification-free genetic detection of single cells
    • Rane, T.D., Zec, H.C., Puleo, C., Lee, A.P. & Wang, T.H. Droplet microfluidics for amplification-free genetic detection of single cells. Lab Chip 12, 3341-3347 (2012
    • (2012) Lab Chip , vol.12 , pp. 3341-3347
    • Rane, T.D.1    Zec, H.C.2    Puleo, C.3    Lee, A.P.4    Wang, T.H.5
  • 32
    • 43949083044 scopus 로고    scopus 로고
    • Development of quantitative cell-based enzyme assays in microdroplets
    • Huebner, A. et al. Development of quantitative cell-based enzyme assays in microdroplets. Anal. Chem. 80, 3890-3896 (2008
    • (2008) Anal. Chem , vol.80 , pp. 3890-3896
    • Huebner, A.1
  • 33
    • 77954190372 scopus 로고    scopus 로고
    • Quantitative cell-based reporter gene assays using droplet-based microfluidics
    • Baret, J.C., Beck, Y., Billas-Massobrio, I., Moras, D. & Griffiths, A.D. Quantitative cell-based reporter gene assays using droplet-based microfluidics. Chem. Biol. 17, 528-536 (2010
    • (2010) Chem. Biol , vol.17 , pp. 528-536
    • Baret, J.C.1    Beck, Y.2    Billas-Massobrio, I.3    Moras, D.4    Griffiths, A.D.5
  • 34
    • 33947184356 scopus 로고    scopus 로고
    • Quantitative detection of protein expression in single cells using droplet microfluidics
    • Huebner, A. et al. Quantitative detection of protein expression in single cells using droplet microfluidics. Chem. Commun. (Camb) 28, 1218-1220 (2007
    • (2007) Chem. Commun. (Camb) , vol.28 , pp. 1218-1220
    • Huebner, A.1
  • 35
    • 55949131242 scopus 로고    scopus 로고
    • The chemistrode: A droplet-based microfluidic device for stimulation and recording with high temporal, spatial, and chemical resolution
    • Chen, D. et al. The chemistrode: A droplet-based microfluidic device for stimulation and recording with high temporal, spatial, and chemical resolution. Proc. Natl. Acad. Sci. USA 105, 16843-16848 (2008
    • (2008) Proc. Natl. Acad. Sci. USA , vol.105 , pp. 16843-16848
    • Chen, D.1
  • 36
    • 79957476318 scopus 로고    scopus 로고
    • A microdroplet dilutor for high-throughput screening
    • Niu, X., Gielen, F., Edel, J.B. & deMello, A.J. A microdroplet dilutor for high-throughput screening. Nat. Chem. 3, 437-442 (2011
    • (2011) Nat. Chem , vol.3 , pp. 437-442
    • Niu, X.1    Gielen, F.2    Edel, J.B.3    De Mello, A.J.4
  • 37
    • 70349682763 scopus 로고    scopus 로고
    • Multi-step microfluidic droplet processing: Kinetic analysis of an in vitro-translated enzyme
    • Mazutis, L. et al. Multi-step microfluidic droplet processing: Kinetic analysis of an in vitro-translated enzyme. Lab Chip 9, 2902-2908 (2009
    • (2009) Lab Chip , vol.9 , pp. 2902-2908
    • Mazutis, L.1
  • 38
    • 79958842027 scopus 로고    scopus 로고
    • Quantitative and sensitive detection of rare mutations using droplet-based microfluidics
    • Pekin, D. et al. Quantitative and sensitive detection of rare mutations using droplet-based microfluidics. Lab Chip 11, 2156-2166 (2011
    • (2011) Lab Chip , vol.11 , pp. 2156-2166
    • Pekin, D.1
  • 39
    • 79958787922 scopus 로고    scopus 로고
    • Multiplex digital PCR: Breaking the one target per color barrier of quantitative PCR
    • Zhong, Q. et al. Multiplex digital PCR: Breaking the one target per color barrier of quantitative PCR. Lab Chip 11, 2167-2174 (2011
    • (2011) Lab Chip , vol.11 , pp. 2167-2174
    • Zhong, Q.1
  • 40
    • 81255175501 scopus 로고    scopus 로고
    • High-throughput droplet digital PCR system for absolute quantitation of DNA copy number
    • Hindson, B.J. et al. High-throughput droplet digital PCR system for absolute quantitation of DNA copy number. Anal. Chem. 83, 8604-8610 (2011
    • (2011) Anal. Chem , vol.83 , pp. 8604-8610
    • Hindson, B.J.1
  • 41
    • 70449339945 scopus 로고    scopus 로고
    • Microdroplet-based PCR enrichment for large-scale targeted sequencing
    • Tewhey, R. et al. Microdroplet-based PCR enrichment for large-scale targeted sequencing. Nat. Biotechnol. 27, 1025-1031 (2009
    • (2009) Nat. Biotechnol , vol.27 , pp. 1025-1031
    • Tewhey, R.1
  • 42
    • 84856010916 scopus 로고    scopus 로고
    • High-resolution dose-response screening using droplet-based microfluidics
    • Miller, O.J. et al. High-resolution dose-response screening using droplet-based microfluidics. Proc. Natl. Acad. Sci. USA 109, 378-383 (2012
    • (2012) Proc. Natl. Acad. Sci. USA , vol.109 , pp. 378-383
    • Miller, O.J.1
  • 43
    • 77951899852 scopus 로고    scopus 로고
    • An automated two-phase microfluidic system for kinetic analyses and the screening of compound libraries
    • Clausell-Tormos, J., Griffiths, A.D. & Merten, C.A. An automated two-phase microfluidic system for kinetic analyses and the screening of compound libraries. Lab Chip 10, 1302-1307 (2010
    • (2010) Lab Chip , vol.10 , pp. 1302-1307
    • Clausell-Tormos, J.1    Griffiths, A.D.2    Merten, C.A.3
  • 44
    • 84859799161 scopus 로고    scopus 로고
    • Rapid screening of antibiotic toxicity in an automated microdroplet system
    • Churski, K. et al. Rapid screening of antibiotic toxicity in an automated microdroplet system. Lab Chip 12, 1629-1637 (2012
    • (2012) Lab Chip , vol.12 , pp. 1629-1637
    • Churski, K.1
  • 45
    • 77749270677 scopus 로고    scopus 로고
    • Ultrahigh-throughput screening in drop-based microfluidics for directed evolution
    • Agresti, J.J. et al. Ultrahigh-throughput screening in drop-based microfluidics for directed evolution. Proc. Natl. Acad. Sci. USA 107, 4004-4009 (2010
    • (2010) Proc. Natl. Acad. Sci. USA , vol.107 , pp. 4004-4009
    • Agresti, J.J.1
  • 46
    • 84865492433 scopus 로고    scopus 로고
    • Picoliter cell lysate assays in microfluidic droplet compartments for directed enzyme evolution
    • Kintses, B. et al. Picoliter cell lysate assays in microfluidic droplet compartments for directed enzyme evolution. Chem. Biol. 19, 1001-1009
    • Chem. Biol , vol.19 , pp. 1001-1009
    • Kintses, B.1
  • 47
    • 77955457208 scopus 로고    scopus 로고
    • Microfluidic compartmentalized directed evolution
    • Paegel, B.M. & Joyce, G.F. Microfluidic compartmentalized directed evolution. Chem. Biol. 17, 717-724 (2010
    • (2010) Chem. Biol , vol.17 , pp. 717-724
    • Paegel, B.M.1    Joyce, G.F.2
  • 48
    • 0036882588 scopus 로고    scopus 로고
    • Perfluorochemical respiratory gas carriers: Benefits to cell culture systems
    • Lowe, K.C. Perfluorochemical respiratory gas carriers: Benefits to cell culture systems. J. Fluorine Chem. 118, 19-26 (2002
    • (2002) J. Fluorine Chem , vol.118 , pp. 19-26
    • Lowe, K.C.1
  • 49
    • 0000992534 scopus 로고
    • The solubility of fluorocarbons
    • Scott, R.L. The solubility of fluorocarbons. J. Am. Chem. Soc. 70, 4090-4093 (1948
    • (1948) J. Am. Chem. Soc , vol.70 , pp. 4090-4093
    • Scott, R.L.1
  • 50
    • 33947455774 scopus 로고
    • The solubility of organic solids in fluorocarbon derivatives
    • Simons, J.H. & Linevsky, M.J. The solubility of organic solids in fluorocarbon derivatives. J. Am. Chem. Soc. 74, 4750-4751 (1952
    • (1952) J. Am. Chem. Soc , vol.74 , pp. 4750-4751
    • Simons, J.H.1    Linevsky, M.J.2
  • 51
    • 0031854986 scopus 로고    scopus 로고
    • Man-made cell-like compartments for molecular evolution
    • Tawfik, D.S. & Griffiths, A.D. Man-made cell-like compartments for molecular evolution. Nat. Biotechnol. 16, 652-656 (1998
    • (1998) Nat. Biotechnol , vol.16 , pp. 652-656
    • Tawfik, D.S.1    Griffiths, A.D.2
  • 52
    • 67649973848 scopus 로고    scopus 로고
    • Fluorescence-activated droplet sorting (FADS): Efficient microfluidic cell sorting based on enzymatic activity
    • Baret, J.C. et al. Fluorescence-activated droplet sorting (FADS): Efficient microfluidic cell sorting based on enzymatic activity. Lab Chip 9, 1850-1858 (2009
    • (2009) Lab Chip , vol.9 , pp. 1850-1858
    • Baret, J.C.1
  • 53
    • 79959926783 scopus 로고    scopus 로고
    • Intracellular protein determination using droplet-based immunoassays
    • Martino, C. et al. Intracellular protein determination using droplet-based immunoassays. Anal. Chem. 83, 5361-5368 (2011
    • (2011) Anal. Chem , vol.83 , pp. 5361-5368
    • Martino, C.1
  • 54
    • 41449096970 scopus 로고    scopus 로고
    • On-chip single-copy real-time reverse-transcription PCR in isolated picoliter droplets
    • Beer, N.R. et al. On-chip single-copy real-time reverse-transcription PCR in isolated picoliter droplets. Anal. Chem. 80, 1854-1858 (2008
    • (2008) Anal. Chem , vol.80 , pp. 1854-1858
    • Beer, N.R.1
  • 55
    • 58149478136 scopus 로고    scopus 로고
    • Continuous-flow polymerase chain reaction of single-copy DNA in microfluidic microdroplets
    • Schaerli, Y. et al. Continuous-flow polymerase chain reaction of single-copy DNA in microfluidic microdroplets. Anal. Chem. 81, 302-306 (2009
    • (2009) Anal. Chem , vol.81 , pp. 302-306
    • Schaerli, Y.1
  • 56
    • 36448957253 scopus 로고    scopus 로고
    • On-chip, real-time, single-copy polymerase chain reaction in picoliter droplets
    • Beer, N.R. et al. On-chip, real-time, single-copy polymerase chain reaction in picoliter droplets. Anal. Chem. 79, 8471-8475 (2007
    • (2007) Anal. Chem , vol.79 , pp. 8471-8475
    • Beer, N.R.1
  • 57
    • 0033772183 scopus 로고    scopus 로고
    • Function-based isolation of novel enzymes from a large library
    • Olsen, M.J. et al. Function-based isolation of novel enzymes from a large library. Nat. Biotechnol. 18, 1071-1074 (2000
    • (2000) Nat. Biotechnol , vol.18 , pp. 1071-1074
    • Olsen, M.J.1
  • 58
    • 33746439461 scopus 로고    scopus 로고
    • High-throughput screening methodology for the directed evolution of glycosyltransferases
    • Aharoni, A. et al. High-throughput screening methodology for the directed evolution of glycosyltransferases. Nat. Methods 3, 609-614 (2006
    • (2006) Nat. Methods , vol.3 , pp. 609-614
    • Aharoni, A.1
  • 59
    • 0034615958 scopus 로고    scopus 로고
    • Monolithic microfabricated valves and pumps by multilayer soft lithography
    • Unger, M.A., Chou, H.P., Thorsen, T., Scherer, A. & Quake, S.R. Monolithic microfabricated valves and pumps by multilayer soft lithography. Science 288, 113-116 (2000
    • (2000) Science , vol.288 , pp. 113-116
    • Unger, M.A.1    Chou, H.P.2    Thorsen, T.3    Scherer, A.4    Quake, S.R.5
  • 60
  • 61
    • 78651304279 scopus 로고    scopus 로고
    • Whole-genome molecular haplotyping of single cells
    • Fan, H.C., Wang, J., Potanina, A. & Quake, S.R. Whole-genome molecular haplotyping of single cells. Nat. Biotechnol. 29, 51-57 (2011
    • (2011) Nat. Biotechnol , vol.29 , pp. 51-57
    • Fan, H.C.1    Wang, J.2    Potanina, A.3    Quake, S.R.4
  • 62
    • 79959830458 scopus 로고    scopus 로고
    • High-throughput analysis of single hematopoietic stem cell proliferation in microfluidic cell culture arrays
    • Lecault, V. et al. High-throughput analysis of single hematopoietic stem cell proliferation in microfluidic cell culture arrays. Nat. Methods 8, 581-586 (2011
    • (2011) Nat. Methods , vol.8 , pp. 581-586
    • Lecault, V.1
  • 63
    • 33745072443 scopus 로고    scopus 로고
    • A microengraving method for rapid selection of single cells producing antigen-specific antibodies
    • Love, J.C., Ronan, J.L., Grotenbreg, G.M., van der Veen, A.G. & Ploegh, H.L. A microengraving method for rapid selection of single cells producing antigen-specific antibodies. Nat. Biotechnol. 24, 703-707 (2006
    • (2006) Nat. Biotechnol , vol.24 , pp. 703-707
    • Love, J.C.1    Ronan, J.L.2    Grotenbreg, G.M.3    Van Der Veen, A.G.4    Ploegh, H.L.5
  • 65
    • 84859887632 scopus 로고    scopus 로고
    • High-throughput analysis of protein-protein interactions in picoliter-volume droplets using fluorescence polarization
    • Choi, J.W., Kang, D.K., Park, H., deMello, A.J. & Chang, S.I. High-throughput analysis of protein-protein interactions in picoliter-volume droplets using fluorescence polarization. Anal. Chem. 84, 3849-3854 (2012
    • (2012) Anal. Chem , vol.84 , pp. 3849-3854
    • Choi, J.W.1    Kang, D.K.2    Park, H.3    De Mello, A.J.4    Chang, S.I.5
  • 66
    • 84863075291 scopus 로고    scopus 로고
    • A homogeneous assay for protein analysis in droplets by fluorescence polarization
    • Joensson, H.N., Zhang, C., Uhlen, M. & Andersson-Svahn, H. A homogeneous assay for protein analysis in droplets by fluorescence polarization. Electrophoresis 33, 436-439 (2012
    • (2012) Electrophoresis , vol.33 , pp. 436-439
    • Joensson, H.N.1    Zhang, C.2    Uhlen, M.3    Andersson-Svahn, H.4
  • 67
    • 51949096580 scopus 로고    scopus 로고
    • Monitoring of real-time streptavidin-biotin binding kinetics using droplet microfluidics
    • Srisa-Art, M., Dyson, E.C., Demello, A.J. & Edel, J.B. Monitoring of real-time streptavidin-biotin binding kinetics using droplet microfluidics. Anal. Chem. 80, 7063-7067 (2008
    • (2008) Anal. Chem , vol.80 , pp. 7063-7067
    • Srisa-Art, M.1    Dyson, E.C.2    Demello, A.J.3    Edel, J.B.4
  • 68
    • 79954554233 scopus 로고    scopus 로고
    • Ultrafast surface enhanced resonance Raman scattering detection in droplet-based microfluidic systems
    • Cecchini, M.P. et al. Ultrafast surface enhanced resonance Raman scattering detection in droplet-based microfluidic systems. Anal. Chem. 83, 3076-3081 (2011
    • (2011) Anal. Chem , vol.83 , pp. 3076-3081
    • Cecchini, M.P.1
  • 70
    • 70349782198 scopus 로고    scopus 로고
    • Detection and analysis of low-abundance cell-surface biomarkers using enzymatic amplification in microfluidic droplets
    • Joensson, H.N. et al. Detection and analysis of low-abundance cell-surface biomarkers using enzymatic amplification in microfluidic droplets. Angew. Chem. Int. Ed. Engl. 48, 2518-2521 (2009
    • (2009) Angew. Chem. Int. Ed. Engl , vol.48 , pp. 2518-2521
    • Joensson, H.N.1
  • 72
    • 0037455351 scopus 로고    scopus 로고
    • Formation Of Dispersions Using "flow focusing" in microchannels
    • Anna, S.L., Bontoux, N. & Stone, H.A. Formation of dispersions using "flow focusing" in microchannels. Appl. Phys. Lett. 82, 364-366 (2003
    • (2003) Appl. Phys. Lett , vol.82 , pp. 364-366
    • Anna, S.L.1    Bontoux, N.2    Stone, H.A.3
  • 73
    • 33644648479 scopus 로고    scopus 로고
    • Formation of droplets and bubbles in a microfluidic T-junction - Scaling and mechanism of break-up
    • Garstecki, P., Fuerstman, M.J., Stone, H.A. & Whitesides, G.M. Formation of droplets and bubbles in a microfluidic T-junction - scaling and mechanism of break-up. Lab Chip 6, 437-446 (2006
    • (2006) Lab Chip , vol.6 , pp. 437-446
    • Garstecki, P.1    Fuerstman, M.J.2    Stone, H.A.3    Whitesides, G.M.4
  • 74
    • 69449101987 scopus 로고    scopus 로고
    • Impact of inlet channel geometry on microfluidic drop formation
    • Abate, A.R. et al. Impact of inlet channel geometry on microfluidic drop formation. Phys. Rev. E 80, 026310 (2009
    • (2009) Phys. Rev , vol.E 80 , pp. 026310
    • Abate, A.R.1
  • 75
    • 0035807144 scopus 로고    scopus 로고
    • Interfacial tension driven monodispersed droplet formation from microfabricated channel array
    • Sugiura, S., Nakajima, M., Iwamoto, S. & Seki, M. Interfacial tension driven monodispersed droplet formation from microfabricated channel array. Langmuir 17, 5562-5566 (2001
    • (2001) Langmuir , vol.17 , pp. 5562-5566
    • Sugiura, S.1    Nakajima, M.2    Iwamoto, S.3    Seki, M.4
  • 76
    • 84855675958 scopus 로고    scopus 로고
    • Surfactants in droplet-based microfluidics
    • Baret, J.C. Surfactants in droplet-based microfluidics. Lab Chip 12, 422-433 (2012
    • (2012) Lab Chip , vol.12 , pp. 422-433
    • Baret, J.C.1
  • 77
    • 66749124156 scopus 로고    scopus 로고
    • Kinetic aspects of emulsion stabilization by surfactants: A microfluidic analysis
    • Baret, J.C., Kleinschmidt, F., El Harrak, A. & Griffiths, A.D. Kinetic aspects of emulsion stabilization by surfactants: A microfluidic analysis. Langmuir 25, 6088-6093 (2009
    • (2009) Langmuir , vol.25 , pp. 6088-6093
    • Baret, J.C.1    Kleinschmidt, F.2    El Harrak, A.3    Griffiths, A.D.4
  • 78
    • 13244289982 scopus 로고    scopus 로고
    • Controlling nonspecific protein adsorption in a plug-based microfluidic system by controlling interfacial chemistry using fluorous-phase surfactants
    • Roach, L.S., Song, H. & Ismagilov, R.F. Controlling nonspecific protein adsorption in a plug-based microfluidic system by controlling interfacial chemistry using fluorous-phase surfactants. Anal. Chem. 77, 785-796 (2005
    • (2005) Anal. Chem , vol.77 , pp. 785-796
    • Roach, L.S.1    Song, H.2    Ismagilov, R.F.3
  • 79
    • 52649177297 scopus 로고    scopus 로고
    • Biocompatible surfactants for water-in-fluorocarbon emulsions
    • Holtze, C. et al. Biocompatible surfactants for water-in-fluorocarbon emulsions. Lab Chip 8, 1632-1639 (2008
    • (2008) Lab Chip , vol.8 , pp. 1632-1639
    • Holtze, C.1
  • 80
    • 81255151150 scopus 로고    scopus 로고
    • Chemical transfection of cells in picoliter aqueous droplets in fluorocarbon oil
    • Chen, F. et al. Chemical transfection of cells in picoliter aqueous droplets in fluorocarbon oil. Anal. Chem. 83, 8816-8820 (2011
    • (2011) Anal. Chem , vol.83 , pp. 8816-8820
    • Chen, F.1
  • 82
    • 84867362611 scopus 로고    scopus 로고
    • Dynamics of molecular transport by surfactants in emulsions
    • Skhiri, Y. Dynamics of molecular transport by surfactants in emulsions. Soft Matter 8, 10618-10627 (2012
    • (2012) Soft Matter , vol.8 , pp. 10618-10627
    • Skhiri, Y.1
  • 83
    • 79954480112 scopus 로고    scopus 로고
    • New generation of amino coumarin methyl sulfonate-based fluorogenic substrates for amidase assays in droplet-based microfluidic applications
    • Woronoff, G. et al. New generation of amino coumarin methyl sulfonate-based fluorogenic substrates for amidase assays in droplet-based microfluidic applications. Anal. Chem. 83, 2852-2857 (2011
    • (2011) Anal. Chem , vol.83 , pp. 2852-2857
    • Woronoff, G.1
  • 84
    • 65249121358 scopus 로고    scopus 로고
    • Controlling the retention of small molecules in emulsion microdroplets for use in cell-based assays
    • Courtois, F. et al. Controlling the retention of small molecules in emulsion microdroplets for use in cell-based assays. Anal. Chem. 81, 3008-3016 (2009
    • (2009) Anal. Chem , vol.81 , pp. 3008-3016
    • Courtois, F.1
  • 85
    • 33750453744 scopus 로고    scopus 로고
    • Cofabrication of electromagnets and microfluidic systems in poly(dimethylsiloxane)
    • Siegel, A.C. et al. Cofabrication of electromagnets and microfluidic systems in poly(dimethylsiloxane). Angew Chem. Int. Ed. Engl. 45, 6877-6882 (2006
    • (2006) Angew Chem. Int. Ed. Engl , vol.45 , pp. 6877-6882
    • Siegel, A.C.1
  • 86
    • 0032682397 scopus 로고    scopus 로고
    • The inertial lift on a spherical particle in a plane Poiseuille flow at large channel Reynolds number
    • Asmolov, E.S. The inertial lift on a spherical particle in a plane Poiseuille flow at large channel Reynolds number. J. Fluid Mech. 381, 63-87 (1999
    • (1999) J. Fluid Mech , vol.381 , pp. 63-87
    • Asmolov, E.S.1
  • 87
    • 37649022478 scopus 로고    scopus 로고
    • Continuous inertial focusing, ordering, and separation of particles in microchannels
    • Di Carlo, D., Irimia, D., Tompkins, R.G. & Toner, M. Continuous inertial focusing, ordering, and separation of particles in microchannels. Proc. Natl. Acad. Sci. USA 104, 18892-18897 (2007
    • (2007) Proc. Natl. Acad. Sci. USA , vol.104 , pp. 18892-18897
    • Di Carlo, D.1    Irimia, D.2    Tompkins, R.G.3    Toner, M.4
  • 88
    • 84864244832 scopus 로고    scopus 로고
    • High-yield cell ordering and deterministic cell-in-droplet encapsulation using Dean flow in a curved microchannel
    • Kemna, E.W.M. et al. High-yield cell ordering and deterministic cell-in-droplet encapsulation using Dean flow in a curved microchannel. Lab Chip 12, 2881-2887 (2012
    • (2012) Lab Chip , vol.12 , pp. 2881-2887
    • Kemna, E.W.M.1
  • 89
    • 0028167730 scopus 로고
    • Iodixanol - A nonionic isosmotic centrifugation medium for the formation of self-generated gradients
    • Ford, T., Graham, J. & Rickwood, D. Iodixanol - a nonionic isosmotic centrifugation medium for the formation of self-generated gradients. Anal. Biochem. 220, 360-366 (1994
    • (1994) Anal. Biochem , vol.220 , pp. 360-366
    • Ford, T.1    Graham, J.2    Rickwood, D.3
  • 90
    • 77952388154 scopus 로고    scopus 로고
    • Use of iodixanol self-generated density gradients to enrich for viable urothelial cells from nonneurogenic and neurogenic bladder tissue
    • Bruce, A.T. et al. Use of iodixanol self-generated density gradients to enrich for viable urothelial cells from nonneurogenic and neurogenic bladder tissue. Tissue Eng. Part C Methods 16, 33-40 (2010
    • (2010) Tissue Eng. Part C Methods , vol.16 , pp. 33-40
    • Bruce, A.T.1
  • 91
    • 0030725671 scopus 로고    scopus 로고
    • A quick, easy and inexpensive method for the isolation of human peripheral blood monocytes
    • Graziani-Bowering, G.M., Graham, J.M. & Filion, L.G. A quick, easy and inexpensive method for the isolation of human peripheral blood monocytes. J. Immunol. Methods 207, 157-168 (1997
    • (1997) J. Immunol. Methods , vol.207 , pp. 157-168
    • Graziani-Bowering, G.M.1    Graham, J.M.2    Filion, L.G.3
  • 94
    • 79960637138 scopus 로고    scopus 로고
    • Statistical modeling of single target cell encapsulation
    • Moon, S., Ceyhan, E., Gurkan, U.A. & Demirci, U. Statistical modeling of single target cell encapsulation. PLoS ONE 6, e21580 (2011
    • (2011) PLoS ONE , vol.6
    • Moon, S.1    Ceyhan, E.2    Gurkan, U.A.3    Demirci, U.4
  • 95
    • 42149135990 scopus 로고    scopus 로고
    • Microfluidic high-throughput encapsulation and hydrodynamic self-sorting of single cells
    • Chabert, M. & Viovy, J.L. Microfluidic high-throughput encapsulation and hydrodynamic self-sorting of single cells. Proc. Natl. Acad. Sci. USA 105, 3191-3196 (2008
    • (2008) Proc. Natl. Acad. Sci. USA , vol.105 , pp. 3191-3196
    • Chabert, M.1    Viovy, J.L.2


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.