Digital microfluidic assay for protein detection

Proceedings of the National Academy of Sciences of the United States of America

Volumn 111, Issue 6, 2014, Pages 2110-2115

  Mok, Janine ^a   Mindrinos, Michael N ^a   Davis, Ronald W ^a   Javanmard, Mehdi ^a  

^a STANFORD UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Bead based assay; Biosensor; Decoupled architecture

Indexed keywords

BEAD-BASED ASSAY; BIOSENSOR; DECOUPLED ARCHITECTURE;

BIOLOGICAL MARKERS; ELECTRIC IMPEDANCE; INTERLEUKIN-6; LIMIT OF DETECTION; MICROFLUIDICS;

EID: 84893851218     PISSN: 00278424     EISSN: 10916490     Source Type: Journal    
DOI: 10.1073/pnas.1323998111     Document Type: Article

References (20)

1. Ge L, Wang SM, Song XR, Ge SG, Yu JH (2012) 3D origami-based multifunctionintegrated immunodevice: Low-cost and multiplexed sandwich chemiluminescence immunoassay on microfluidic paper-based analytical device. Lab Chip 12(17): 3150-3158.
2. Diercks AH, et al. (2009) A microfluidic device for multiplexed protein detection in nano-liter volumes. Anal Biochem 386(1):30-35.
3. Hughes AJ, Herr AE (2012) Microfluidic Western blotting. Proc Natl Acad Sci USA 109(52):21450-21455.
4. Herr AE, et al. (2007) Microfluidic immunoassays as rapid saliva-based clinical diagnostics. Proc Natl Acad Sci USA 104(13):5268-5273.
5. Kai J, et al. (2012) A novel microfluidic microplate as the next generation assay platform for enzyme linked immunoassays (ELISA). Lab Chip 12(21):4257-4262.
6. Kim Y-G, Moon S, Kuritzkes DR, Demirci U (2009) Quantum dot-based HIV capture and imaging in a microfluidic channel. Biosens Bioelectron 25(1):253-258.
7. Dunne J, Reardon H, Trinh V, Li E, Farinas J (2004) Comparison of on-chip and off-chip microfluidic kinase assay formats. Assay Drug Dev Technol 2(2):121-129.
8. Lee JH, Cosgrove BD, Lauffenburger DA, Han J (2009) Microfluidic concentrationenhanced cellular kinase activity assay. J Am Chem Soc 131(30):10340-10341.
9. Mulvaney SP, et al. (2007) Rapid, femtomolar bioassays in complex matrices combining microfluidics and magnetoelectronics. Biosens Bioelectron 23(2):191-200.
10. Mulvaney SP, Myers KM, Sheehan PE, Whitman LJ (2009) Attomolar protein detection in complex sample matrices with semi-homogeneous fluidic force discrimination assays. Biosens Bioelectron 24(5):1109-1115.
11. Tseng D, et al. (2010) Lensfree microscopy on a cellphone. Lab Chip 10(14):1787-1792.
12. Berggren C, Stalhandske P, Brundell J, Johansson G (1999) A feasibility study of a capacitive biosensor for direct detection of DNA hybridization. Electroanalysis 11(3): 156-160.
13. Esfandyarpour R, Esfandyarpour H, Harris JS, Davis RW (2013) Simulation and fabrication of a new novel 3D injectable biosensor for high throughput genomics and proteomics in a lab-on-a-chip device. Nanotechnology 24(46):465301.
14. Esfandyarpour R, Esfandyarpour H, Javanmard M, Harris JS, Davis RW (2013) Microneedle biosensor: A method for direct label-free real time protein detection. Sens Actuators B Chem 177:848-855.
15. Esfandyarpour R, et al. (2013) Label-free electronic probing of nucleic acids and proteins at the nanoscale using the nanoneedle biosensor. Biomicrofluidics 7(4): 044114.
16. Zheng G, Patolsky F, Cui Y, Wang WU, Lieber CM (2005) Multiplexed electrical detection of cancer markers with nanowire sensor arrays. Nat Biotechnol 23(10): 1294-1301.
17. Gaster RS, et al. (2009) Matrix-insensitive protein assays push the limits of biosensors in medicine. Nat Med 15(11):1327-1332.
18. Kim J, Jensen EC, Megens M, Boser B, Mathies RA (2011) Integrated microfluidic bioprocessor for solid phase capture immunoassays. Lab Chip 11(18):3106-3112.
19. Javanmard M, et al. (2009) Electrical detection of protein biomarkers using bioactivated microfluidic channels. Lab Chip 9(10):1429-1434.
20. Manickam A, Chevalier A, McDermott M, Ellington AD (2010) A CMOS electrochemical impedance spectroscopy biosensor array for label-free biomolecular detection. IEEE Trans Biomed Circuits Syst 4(6):379-390.