Microfluidic isolation of highly pure embryonic stem cells using feeder-separated co-culture system

Scientific Reports

Volumn 3, Issue , 2013, Pages

  Chen, Qiushui ^a   Wu, Jing ^a   Zhuang, Qichen ^a   Lin, Xuexia ^a   Zhang, Jie ^a   Lin, Jin Ming ^a  

^a TSINGHUA UNIVERSITY   (China)

Author keywords

Bioinspired Materials; Lab On A Chip; Regeneration; Stem Cell Biotechnology; Subject Areas

Indexed keywords

ALKALINE PHOSPHATASE; DIMETICONE; OCTAMER TRANSCRIPTION FACTOR 4;

ANIMAL; ANIMAL EMBRYO; CELL DIFFERENTIATION; CELL PROLIFERATION; CELL SEPARATION; CELL SURVIVAL; CHEMISTRY; COCULTURE; CYTOLOGY; DEVICES; EMBRYONIC STEM CELL; ENZYMOLOGY; EQUIPMENT DESIGN; FEEDER CELL; FIBROBLAST; METABOLISM; MICROFLUIDICS; MICROTECHNOLOGY; MOUSE; PLURIPOTENT STEM CELL; PROCEDURES; ARTICLE; EQUIPMENT; METHODOLOGY;

ALKALINE PHOSPHATASE; ANIMALS; CELL DIFFERENTIATION; CELL PROLIFERATION; CELL SEPARATION; CELL SURVIVAL; COCULTURE TECHNIQUES; DIMETHYLPOLYSILOXANES; EMBRYO, MAMMALIAN; EMBRYONIC STEM CELLS; EQUIPMENT DESIGN; FEEDER CELLS; FIBROBLASTS; MICE; MICROFLUIDICS; MICROTECHNOLOGY; OCTAMER TRANSCRIPTION FACTOR-3; PLURIPOTENT STEM CELLS;

EID: 84882746521     PISSN: None     EISSN: 20452322     Source Type: Journal    
DOI: 10.1038/srep02433     Document Type: Article

References (34)

1. Lutolf, M. P., Gilbert, P. M. & Blau, H. M. Designing materials to direct stem-cellfate. Nature 462, 433-441 (2009).
2. Badylak, S. F. & Nerem, R. M. Progress in tissue engineering and regenerativemedicine. Proc. Natl. Acad. Sci. U. S. A. 107, 3285-3286 (2010).
3. Seliktar, D. Designing cell-compatible hydrogels for biomedical applications.Science 336, 1124-1128 (2012).
4. Sneddon, J. B., Borowiak, M. & Melton, D. A. Self-renewal of embryonic-stemcell-derived progenitors by organ-matched mesenchyme. Nature 491, 765-768(2012).
5. Sueblinvong, V. & Weiss, D. J. Stem cells and cell therapy approaches in lungbiology and diseases. Transl. Res. 156, 188-205 (2010).
6. Shi, Y. et al. Induction of pluripotent stem cells from mouse embryonic fibroblastsby Oct4 and Klf4 with small-molecule compounds. Cell Stem Cell 3, 568-574(2008).
7. Sato, N.,Meijer, L., Skaltsounis, L., Greengard, P. & Brivanlou, A. H. Maintenanceof pluripotency in human and mouse embryonic stem cells through activation ofWnt signaling by a pharmacological GSK-3-specific inhibitor. Nat. Med. 2004, 10,55-63 (2008). (Pubitemid 38524699)
8. Gobaa, S. et al. Artificial niche microarrays for probing single stem cell fate in highthroughput. Nat. Methods 8, 949-955 (2011).
9. Draper, J. S. et al. Recurrent gain of chromosomes 17q and 12 in cultured humanembryonic stem cells. Nat. Biotechnol. 22, 53-54 (2004). (Pubitemid 38057956)
10. Xu, C. et al. Feeder-free growth of undifferentiated human embryonic stem cells.Nat. Biotechnol. 19, 971-974 (2001). (Pubitemid 32947578)
11. Singh, A. et al.Adhesion strength-based, label-free isolation of human pluripotentstem cells. Nat. Methods 10, 438-444 (2013).
12. Shin, Y. et al.Microfluidic assay for simultaneous culture of multiple cell types onsurfaces or within hydrogels. Nat. Protoc. 7, 1247-1259 (2012).
13. Wu, J., Chen, Q., Liu, W., Zhang, Y. & Lin, J.-M. Cytotoxicity of quantum dotsassay on a microfluidic 3D-culture device based on modeling diffusion processbetween blood vessels and tissues. Lab Chip 12, 3474-3480 (2012).
14. Chen, Q., Wu, J., Zhang, Y. & Lin, J.-M. Qualitative and quantitative analysis oftumor cell metabolism via stable isotope labeling assisted microfluidic chipelectrospray ionization mass spectrometry. Anal. Chem. 84, 1695-1701 (2012).
15. Gao, D., Liu, H., Lin, J.-M., Wang, Y. & Jiang, Y. Characterization of drugpermeability in Caco-2 monolayers by mass spectrometry on a membrane-basedmicrofluidic device. Lab Chip 13, 978-985 (2013).
16. Chen, Q., Wu, J., Zhang, Y., Lin, Z. & Lin, J.-M. Targeted isolation and analysis ofsingle tumor cells with aptamer-encoded microwell array on microfluidic device.Lab Chip 12, 5180-5185 (2012).
17. Schirhagl, R., Fuereder, I., Hall, E. W., Medeiros, B. C. & Zare, R. N. Microfluidicpurification and analysis of hematopoietic stem cells from bone marrow. Lab Chip11, 3130-3135 (2011).
18. Kim, C. et al. 3-Dimensional cell culture for on-chip differentiation of stem cells inembryoid body. Lab Chip 11, 874-882 (2011).
19. Khademhosseini, A., Langer, R., Borenstein, J. & Vacanti, J. P. Microscaletechnologies for tissue engineering and biology. Proc. Natl. Acad. Sci. U. S. A. 103,2480-2487 (2006).
20. Huh, D. et al. Reconstituting Organ-Level Lung Functions on a Chip. Science 328,1662-1668 (2010).
21. El-Ali, J., Sorger, P. K. & Jensen, K. F. Cells on chips. Nature 442, 403-411 (2006). (Pubitemid 44264784)
22. Zhu, Z. et al.Highly sensitive and quantitative detection of rare pathogens throughagarose droplet microfluidic emulsion PCR at the single-cell level. Lab Chip 12,3907-3913 (2012).
23. Gu, S. et al. Multifunctional picoliter droplet manipulation platform and itsapplication in single cell analysis. Anal. Chem. 83, 7570-7576 (2011).
24. Zhang, H. et al. DNA mutation detection with chip-based temperature gradientcapillary electrophoresis using a slantwise radiative heating system. Lab Chip 7,1162-1170 (2007). (Pubitemid 47295967)
25. Kawada, J. et al. Spatiotemporally controlled delivery of soluble factors for stemcell differentiation. Lab Chip 12, 4508-4515 (2012).
26. Gupta, K. et al. Lab-on-a-chip devices as an emerging platform for stem cellbiology. Lab Chip 10, 2019-2031 (2010).
27. Ma,H., Zhang, M. &Qin, J. Probing the role of mesenchymal stem cells in salivarygland cancer on biomimetic microdevices. Integr. Biol. 4, 522-530 (2012).
28. Kamei, K. et al. An integrated microfluidic culture device for quantitative analysisof human embryonic stem cells. Lab Chip 9, 555-563 (2009).
29. Lecault, V. et al. High-throughput analysis of single hematopoietic stem cellproliferation in microfluidic cell culture arrays. Nat. Methods 8, 581-586 (2011).
30. Anil Kumar, H. et al. A modular approach to create a neurovascular unit-on-achip.Lab Chip 13, 542-553(2012).
31. Kim, H. J., Huh, D.,Hamilton, G. & Ingber, D. E. Human gut-on-a-chip inhabitedby microbial flora that experiences intestinal peristalsis-like motions and flow.Lab Chip 12, 2165-2174 (2012).
32. Regehr, K. J. et al. Biological implications of polydimethylsiloxane- basedmicrofluidic cell culture. Lab Chip 9, 2132-2139 (2009).
33. Khademhosseini, A. et al. Co-culture of human embryonic stem cells with murineembryonic fibroblasts on microwell-patterned substrates. Biomaterials 27,5968-5977 (2006). (Pubitemid 44414975)
34. Karwacki-Neisius, V. et al. Reduced Oct4 expression directs a robust pluripotentstate with distinct signaling activity and increased enhancer occupancy by Oct4and Nanog. Cell Stem Cell 12, 531-545 (2013).