Hydrogen exchange mass spectrometry reveals protein interfaces and distant dynamic coupling effects during the reversible self-association of an IgG1 monoclonal antibody

mAbs

Volumn 7, Issue 3, 2015, Pages 525-539

  Arora, Jayant ^a   Hickey, John M ^a   Majumdar, Ranajoy ^a,c   Esfandiary, Reza ^b   Bishop, Steven M ^b   Samra, Hardeep S ^b   Middaugh, C Russell ^a   Weis, David D ^a   Volkin, David B ^a  

^a UNIVERSITY OF KANSAS   (United States)

^b MEDIMMUNE   (United States)

^c ELI LILLY AND COMPANY   (United States)

Author keywords

Aggregation; Flexibility; High protein concentration; Hydrogen exchange; Immunoglobulin G1; Mass spectrometry; Monoclonal antibody; Protein protein interactions; Reversible self association; Stability

Indexed keywords

DEUTERIUM; HYDROGEN; IMMUNOGLOBULIN G1; MONOCLONAL ANTIBODY; PEPTIDE; COMPLEMENTARITY DETERMINING REGION; IMMUNOGLOBULIN G;

ABSORPTION SPECTROSCOPY; AMINO ACID SEQUENCE; ARTICLE; CIRCULAR DICHROISM; COMPLEMENTARITY DETERMINING REGION; CROSS LINKING; FREEZE DRYING; HINGE REGION; HYDROGEN EXCHANGE MASS SPECTROMETRY; LIGHT CHAIN; LIGHT SCATTERING; MASS SPECTROMETRY; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN AGGREGATION; PROTEIN ASSEMBLY; PROTEIN PROCESSING; PROTEIN PROTEIN INTERACTION; SIZE EXCLUSION CHROMATOGRAPHY; VISCOSITY; ANIMAL; CHEMISTRY; DEUTERIUM HYDROGEN EXCHANGE; IMMUNOLOGY; MOLECULAR DYNAMICS; MOUSE;

ANIMALS; ANTIBODIES, MONOCLONAL, MURINE-DERIVED; COMPLEMENTARITY DETERMINING REGIONS; DEUTERIUM EXCHANGE MEASUREMENT; IMMUNOGLOBULIN G; MASS SPECTROMETRY; MICE; MOLECULAR DYNAMICS SIMULATION;

EID: 84938844658     PISSN: 19420862     EISSN: 19420870     Source Type: Journal    
DOI: 10.1080/19420862.2015.1029217     Document Type: Article

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