Structure-based engineering of a monoclonal antibody for improved solubility

Protein Engineering, Design and Selection

Volumn 23, Issue 8, 2010, Pages 643-651

  Wu, Sheng Jiun ^a   Luo, Jinquan ^a   O'Neil, Karyn T ^a   Kang, James ^b   Lacy, Eilyn R ^a   Canziani, Gabriela ^a   Baker, Audrey ^a   Huang, Maggie ^a   Tang, Qing Mike ^a   Raju, T Shantha ^a   Jacobs, Steven A ^a   Teplyakov, Alexey ^a   Gilliland, Gary L ^a   Feng, Yiqing ^a  

^a Biologics Research   (United States)

^b REGENERON PHARMACEUTICALS INC   (United States)

Author keywords

antibody; N glycosylation; Protein engineering; Solubility; structure based design

Indexed keywords

ANTIGENS; BINDING ENERGY; BIOCHEMICAL ENGINEERING; CARBOHYDRATES; DRUG PRODUCTS; ENGINEERING; ESTERIFICATION; GENETIC ENGINEERING; GLYCOSYLATION; HYDROPHOBICITY; MONOCLONAL ANTIBODIES; PROTEINS; SURFACE PLASMON RESONANCE;

N-GLYCOSYLATION; PROTEIN ENGINEERING; STRUCTURE-BASED; STRUCTURE-BASED DESIGN;

SOLUBILITY;

INTERLEUKIN 13; MONOCLONAL ANTIBODY; MONOCLONAL ANTIBODY CNT0607; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; ANTIBODY ENGINEERING; ANTIBODY STRUCTURE; ANTIGEN BINDING; ARTICLE; BINDING AFFINITY; CARBOHYDRATE ANALYSIS; HEAVY CHAIN; HUMAN; HYDROPHOBICITY; ISOELECTRIC POINT; MASS SPECTROMETRY; MUTAGENESIS; PEPTIDE MAPPING; PRIORITY JOURNAL; PROTEIN AGGREGATION; PROTEIN FOLDING; PROTEIN GLYCOSYLATION; PROTEIN VARIANT; SEQUENCE ANALYSIS; SOLUBILITY; STRUCTURE ANALYSIS; ANTAGONISTS AND INHIBITORS; BINDING SITE; CHEMICAL PHENOMENA; CHEMICAL STRUCTURE; CHEMISTRY; DIFFERENTIAL SCANNING CALORIMETRY; GENETICS; ISOELECTRIC FOCUSING; METABOLISM; MOLECULAR GENETICS; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROCEDURES; PROTEIN CONFORMATION; PROTEIN ENGINEERING; PROTEIN MULTIMERIZATION; PROTEIN STABILITY; TEMPERATURE;

AMINO ACID SEQUENCE; ANTIBODIES, MONOCLONAL; BINDING SITES; CALORIMETRY, DIFFERENTIAL SCANNING; ELECTROPHORESIS, POLYACRYLAMIDE GEL; HUMANS; HYDROPHOBIC AND HYDROPHILIC INTERACTIONS; INTERLEUKIN-13; ISOELECTRIC FOCUSING; ISOELECTRIC POINT; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; PEPTIDE MAPPING; PROTEIN CONFORMATION; PROTEIN ENGINEERING; PROTEIN MULTIMERIZATION; PROTEIN STABILITY; SOLUBILITY; TEMPERATURE;

EID: 77955002341     PISSN: 17410126     EISSN: 17410134     Source Type: Journal    
DOI: 10.1093/protein/gzq037     Document Type: Article

References (59)

1. Andrews, B.A., Schmidt, A.S. and Asenjo, J.A. (2005) Biotechnol. Bioeng., 90, 380-390.
2. Ashbaugh, H.S. and Hatch, H.W. (2008) J. Am. Chem. Soc., 130, 9536-9542.
3. Bagby, S., Tong, K.I. and Ikura, M. (2001) Methods Enzymol., 339, 20-41.
4. Chothia, C., Gelfand, I. and Kister, A. (1998) J. Mol. Biol., 278, 457-479.
5. Christ, D., Famm, K. and Winter, G. (2007) Protein Eng. Des. Sel., 20, 413-416.
6. Co, M.S., Scheinberg, D.A., Avdalovic, N.M., McGraw, K., Vasquez, M., Caron, P.C. and Queen, C. (1993) Mol. Immunol., 30, 1361-1367.
7. Coloma, M.J., Trinh, R.K., Martinez, A.R. and Morrison, S.L. (1999) J. Immunol., 162, 2162-2170.
8. Dale, G.E., Oefner, C. and D'Arcy, A. (2003) J. Struct. Biol., 142, 88-97.
9. Demeule, B., Lawrence, M.J., Drake, A.F., Gurny, R. and Arvinte, T. (2007) Biochim. Biophys. Acta, 1774, 146-153.
10. Derewenda, Z.S. (2004) Methods, 34, 354-363.
11. Dorai, H., et al. (2009) Biotechnol. Bioeng., 103, 162-176.
12. Ewert, S., Huber, T., Honegger, A. and Pluckthun, A. (2003) J. Mol. Biol., 325, 531-553.
13. Famm, K. and Winter, G. (2006) Protein Eng. Des. Sel., 19, 479-481.
14. Famm, K., Hansen, L., Christ, D. and Winter, G. (2008) J. Mol. Biol., 376, 926-931.
15. Fowler, S.B., Poon, S., Muff, R., Chiti, F., Dobson, C.M. and Zurdo, J. (2005) Proc. Natl Acad. Sci. USA, 102, 10105-10110.
16. Garber, E. and Demarest, S.J. (2007) Biochem. Biophys. Res. Commun., 355, 751-757.
17. Haire, l.F. and Blow, D.B. (2001) J. Cryst. Growth, 232, 17-20.
18. Hmila, I., Abdallah, R.B., Saerens, D., Benlasfar, Z., Conrath, K., Ayeb, M.E., Muyldermans, S. and Bouhaouala-Zahar, B. (2008) Mol. Immunol., 45, 3847-3856.
19. Jacobs, S.A., Wu, S.J., Feng, Y., Bethea, D. and O'Neil, K.T. (2010) Pharm. Res., 27, 65-71.
20. Jespers, L., Schon, O., Famm, K. and Winter, G. (2004) Nat. Biotechnol., 22, 1161-1165.
21. Kerns, E.H. and Di, L. (2003) Drug Discov. Today, 8, 316-323.
22. Khurana, S., Raghunathan, V. and Salunke, D.M. (1997) Biochem. Biophys. Res. Commun., 234, 465-469.
23. Laemmli, U.K. (1970) Nature, 227, 680-685.
24. Lee, E.N., Kim, Y.M., Lee, H.J., Park, S.W., Jung, H.Y., Lee, J.M., Ahn, Y.H. and Kim, J. (2005) Pharm. Res., 22, 1735-1746.
25. Lefranc, M.P., et al. (2009) Nucleic Acids Res., 37, D1006-D1012.
26. Leibiger, H., Wustner, D., Stigler, R.D. and Marx, U. (1999) Biochem. J., 338, 529-538.
27. Li, H., et al. (2006) Nat. Biotechnol., 24, 210-215.
28. Lienqueo, M.E., Mahn, A., Navarro, G., Salgado, J.C., Perez-Acle, T., Rapaport, I. and Asenjo, J.A. (2006) J. Mol. Recognit., 19, 260-269.
29. MacCallum, R.M., Martin, A.C. and Thornton, J.M. (1996) J. Mol. Biol., 262, 732-745.
30. Moore, P.A. and Kery, V. (2009) Methods Mol. Biol., 498, 309-314.
31. Mosavi, L.K. and Peng, Z.Y. (2003) Protein Eng., 16, 739-745.
32. Myszka, D.G. (1999) J. Mol. Recognit., 12, 279-284.
33. Nasreen, A., Vogt, M., Kim, H.J., Eichinger, A. and Skerra, A. (2006) Protein Sci., 15, 190-199.
34. Nimmerjahn, F. and Ravetch, J.V. (2008) Annu. Rev. Immunol., 26, 513-533.
35. Patra, A.K. and Udgaonkar, J.B. (2007) Biochemistry, 46, 11727-11743.
36. Pawar, A.P., Dubay, K.F., Zurdo, J., Chiti, F., Vendruscolo, M. and Dobson, C.M. (2005) J. Mol. Biol., 350, 379-392.
37. Qian, J., Liu, T., Yang, L., Daus, A., Crowley, R. and Zhou, Q. (2007) Anal. Biochem., 364, 8-18.
38. Raju, T.S. (2008) Curr. Opin. Immunol., 20, 471-478.
39. Raju, T.S. and Scallon, B. (2007) Biotechnol. Prog., 23, 964-971.
40. Rangwala, H. and Karypis, G. (2007) Bioinformatics, 23, e17-e23.
41. Riechmann, L. and Winter, G. (2006) J. Mol. Biol., 363, 460-468.
42. Rothe, C., et al. (2008) J. Mol. Biol., 376, 1182-1200.
43. Rudd, P.M., Wormald, M.R., Harvey, D.J., Devasahayam, M., McAlister, M.S., Brown, M.H., Davis, S.J., Barclay, A.N. and Dwek, R.A. (1999) Glycobiology, 9, 443-458.
44. Scallon, B.J., Tam, S.H., McCarthy, S.G., Cai, A.N. and Raju, T.S. (2007) Mol. Immunol., 44, 1524-1534.
45. Shirai, T., Suzuki, A., Yamane, T., Ashida, T., Kobayashi, T., Hitomi, J. and Ito, S. (1997) Protein Eng., 10, 627-634.
46. Sinclair, A.M. and Elliott, S. (2005) J. Pharm. Sci., 94, 1626-1635.
47. Spiegelberg, H.L., Abel, C.A., Fishkin, B.G. and Grey, H.M. (1970) Biochemistry, 9, 4217-4223.
48. Strickler, S.S., Gribenko, A.V., Gribenko, A.V., Keiffer, T.R., Tomlinson, J., Reihle, T., Loladze, V.V. and Makhatadze, G.I. (2006) Biochemistry, 45, 2761-2766.
49. Swencki-Underwood, B., Mills, J.K., Vennarini, J., Boakye, K., Luo, J., Pomerantz, S., Cunningham, M.R., Farrell, F.X., Naso, M.F. and Amegadzie, B. (2008) Protein Expr. Purif., 57, 312-319.
50. Tan, P.H., Chu, V., Stray, J.E., Hamlin, D.K., Pettit, D., Wilbur, D.S., Vessella, R.L. and Stayton, P.S. (1998) Immunotechnology, 4, 107-114.
51. Teplyakov, A., Obmolova, G., Wu, S.J., Luo, J., Kang, J., O'Neil, K. and Gilliland, G.L. (2009) J. Mol. Biol., 389, 115-123.
52. To, B.C. and Lenhoff, A.M. (2007a) J. Chromatogr. A, 1141, 191-205.
53. To, B.C. and Lenhoff, A.M. (2007b) J. Chromatogr. A, 1141, 235-243.
54. Trevino, S.R., Scholtz, J.M. and Pace, C.N. (2007) J. Mol. Biol., 366, 449-460.
55. van Reis, R. and Zydney, A. (2001) Curr. Opin. Biotechnol., 12, 208-211.
56. Veronese, F.M. and Mero, A. (2008) BioDrugs, 22, 315-329.
57. Wallick, S.C., Kabat, E.A. and Morrison, S.L. (1988) J. Exp. Med., 168, 1099-1109.
58. Wiecek, A., Nieszporek, T. and Chudek, J. (2007) Prilozi., 28, 225-237.
59. Wright, A., Tao, M.H., Kabat, E.A. and Morrison, S.L. (1991) EMBO J., 10, 2717-2723.