Minimizing carry-over in an online pepsin digestion system used for the H/D exchange mass spectrometric analysis of an IgG1 monoclonal antibody

Journal of the American Society for Mass Spectrometry

Volumn 23, Issue 12, 2012, Pages 2140-2148

  Majumdar, Ranajoy ^a   Manikwar, Prakash ^a   Hickey, John M ^a   Arora, Jayant ^a   Middaugh, C Russell ^a   Volkin, David B ^a   Weis, David D ^a  

^a UNIVERSITY OF KANSAS   (United States)

Author keywords

Carry over; H D exchange mass spectrometry; On line digestion; Pepsin; Proteolysis

Indexed keywords

CARRY-OVER; GUANIDINE HYDROCHLORIDE; H/D EXCHANGE; MASS SPECTROMETRIC ANALYSIS; ON-LINE DIGESTION; PEPSIN; PHOSPHATE BUFFERS; WASHING PROCEDURES;

DIGESTIVE SYSTEM; MASS SPECTROMETRY; MONOCLONAL ANTIBODIES; PEPTIDES; PROTEOLYSIS; WASHING;

BIOLOGICAL MATERIALS;

2 PROPANOL; ACETIC ACID; ACETONITRILE; AMIDE; GUANIDINE; IMMUNOGLOBULIN G1; MONOCLONAL ANTIBODY; PEPSIN A; PHOSPHATE; WATER; IMMUNOGLOBULIN G; PEPTIDE FRAGMENT;

ANALYTICAL EQUIPMENT; ARTICLE; CONTROLLED STUDY; ENZYME IMMOBILIZATION; ENZYME SPECIFICITY; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; LIQUID CHROMATOGRAPHY; MASS SPECTROMETER; MASS SPECTROMETRY; MOLECULAR WEIGHT; ONLINE SYSTEM; PH; PROTEIN ANALYSIS; TEMPERATURE; CHEMISTRY; DEUTERIUM HYDROGEN EXCHANGE; ELECTROSPRAY MASS SPECTROMETRY; EQUIPMENT DESIGN; INSTRUMENTATION; METABOLISM; METHODOLOGY; REPRODUCIBILITY; STATISTICAL MODEL;

ANTIBODIES, MONOCLONAL; CHROMATOGRAPHY, LIQUID; DEUTERIUM EXCHANGE MEASUREMENT; EQUIPMENT DESIGN; HYDROGEN-ION CONCENTRATION; IMMUNOGLOBULIN G; LINEAR MODELS; PEPSIN A; PEPTIDE FRAGMENTS; REPRODUCIBILITY OF RESULTS; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION; TEMPERATURE;

EID: 84873954014     PISSN: 10440305     EISSN: 18791123     Source Type: Journal    
DOI: 10.1007/s13361-012-0485-9     Document Type: Article

References (28)

1. Zhang, Z., Smith, D.L.: Determination of amide hydrogen exchange by mass spectrometry: a new tool for protein structure elucidation. Protein Sci. 2, 522-531 (1993)
2. Englander, S.W.: Hydrogen exchange and mass spectrometry: a historical perspective. J. Am. Soc. Mass Spectrom. 17, 1481-1489 (2006)
3. Konermann, L., Tong, X., Pan, Y.: Protein structure and dynamics studied by mass spectrometry: H/D exchange, hydroxyl radical labeling, and related approaches. J. Mass Spectrom. 43, 1021-1036 (2008)
4. Engen, J.R.: Analysis of protein conformation and dynamics by hydrogen/deuterium exchange MS. Anal. Chem. 81, 7870-7875 (2009)
5. Ghaemmaghami, S., Fitzgerald, M.C., Oas, T.G.: A quantitative, high-throughput screen for protein stability. Proc. Natl. Acad. Sci. U.S.A. 97, 8296-8301 (2000)
6. Zhang, J., Chalmers, M.J., Stayrook, K.R., Burris, L.L., Garcia-Ordonez, R.D., Pascal, B.D., Burris, T.P., Dodge, J.A., Griffin, P.R.: Hydrogen/deuterium exchange reveals distinct agonist/partial agonist receptor dynamics within vitamin D receptor/retinoid X receptor heterodimer. Structure 18, 1332-1341 (2010)
7. Morgan, C.R., Miglionico, B.V., Engen, J.R.: Effects of HIV-1 Nef on human N-myristoyltransferase 1. Biochemistry 50, 3394-3403 (2011)
8. Chung, K.Y., Rasmussen, S.G.F., Liu, T., Li, S., DeVree, B.T., Chae, P.S., Calinski, D., Kobilka, B.K., Woods, V.L., Sunahara, R.K.: Conformational changes in the G protein Gs induced by the β2 adrenergic receptor. Nature 477, 611-615 (2011)
9. Houde, D., Arndt, J., Domeier, W., Berkowitz, S., Engen, J.R.: Characterization of IgG1 conformation and conformational dynamics by hydrogen/deuterium exchange mass spectrometry. Anal. Chem. 81, 2644-2651 (2009)
10. Keppel, T.R., Howard, B.A., Weis, D.D.: Mapping unstructured regions and synergistic folding in intrinsically disordered proteins with amide H/D exchange mass spectrometry. Biochemistry 50, 8722-8732 (2011)
11. Rumi-Masante, J., Rusinga, F.I., Lester, T.E., Dunlap, T.B., Williams, T.D., Dunker, A.K., Weis, D.D., Creamer, T.P.: Structural basis for activation of calcineurin by calmodulin. J. Mol. Biol. 415, 307-317 (2012)
12. Bennett, M.J., Barakat, K., Huzil, J.T., Tuszynski, J., Schriemer, D.C.: Discovery and characterization of the laulimalide-microtubule binding bode by mass shift perturbation mapping. Chem. Biol. 17, 725-734 (2010)
13. Rosa, J.J., Richards, F.M.: An experimental procedure for increasing the structural resolution of chemical hydrogen-exchange measurements on proteins: Application to ribonuclease S peptide. J. Mol. Biol. 133, 399-416 (1979)
14. Wang, L., Pan, H., Smith, D.L.: Hydrogen exchange-mass spectrometry: optimization of digestion conditions. Mol. Cell. Proteom. 1, 132-138 (2002)
15. Fang, J., Rand, K.D., Beuning, P.J., Engen, J.R.: False EX1 signatures caused by sample carryover during HX MS analyses. Int. J. Mass Spectrom. 302, 19-25 (2011)
16. Burkitt, W., Domann, P., O'Connor, G.: Conformational changes in oxidatively stressed monoclonal antibodies studied by hydrogen exchange mass spectrometry. Protein Sci. 19, 826-835 (2010)
17. Houde, D., Peng, Y., Berkowitz, S.A., Engen, J.R.: Post-translational modifications differentially affect IgG1 conformation and receptor binding. Mol. Cell. Proteom. 9, 1716-1728 (2010)
18. Zhang, A., Singh, S.K., Shirts, M.R., Kumar, S., Fernandez, E.J.: Distinct aggregation mechanisms of monoclonal antibody under thermal and freeze-thaw stresses revealed by hydrogen exchange. Pharm. Res. 29, 236-250 (2012)
19. Keppel, T.R., Jacques, M.E., Young, R.W., Ratzlaff, K.L., Weis, D.D.: An efficient and inexpensive refrigerated LC system for H/D exchange mass spectrometry. J. Am. Soc. Mass Spectrom. 22, 1472-1476 (2011)
20. Kyte, J., Doolittle, R.F.: A simple method for displaying the hydropathic character of a protein. J. Mol. Biol. 157, 105-132 (1982)
21. Gasteiger, E., Gattiker, A., Hoogland, C., Ivanyi, I., Appel, R.D., Bairoch, A.: ExPASy: the proteomics server for in-depth protein knowledge and analysis. Nucleic Acids Res. 31, 3784-3788 (2003)
22. Haeckel, R.: Recommendations for definition and determination of carry-over effects. J. Automatic Chem. 10, 181-183 (1988)
23. Zeng, W., Musson, D.G., Fisher, A.L., Wang, A.Q.: A new approach for evaluating carryover and its influence on quantitation in high-performance liquid chromatography and tandem mass spectrometry assay. Rapid Commun. Mass Spectrom. 20, 635-640 (2006)
24. Mitulović, G., Stingl, C., Steinmacher, I., Hudecz, O., Hutchins, J.R.A., Peters, J.-M., Mechtler, K.: Preventing carryover of peptides and proteins in Nano LC-MS separations. Anal. Chem. 81, 5955-5960 (2009)
25. Ng, K.K.S., Petersen, J.F.W., Cherney, M.M., Garen, C., Zalatoris, J.J., Rao-Naik, C., Dunn, B.M., Martzen, M.R., Peanasky, R.J., James, M.N.G.: Structural basis for the inhibition of porcine pepsin by Ascaris pepsin inhibitor-3. Nat. Struct. Mol. Biol. 7, 653-657 (2000)
26. Brier, S., Maria, G., Carginale, V., Capasso, A., Wu, Y., Taylor, R.M., Borotto, N.B., Capasso, C., Engen, J.R.: Purification and characterization of pepsins-A1 and A2 from the Antarctic rock cod Trematomus bernacchii. FEBS J. 274, 6152-6166 (2007)
27. Zhang, H.-M., Kazazic, S., Schaub, T.M., Tipton, J.D., Emmett, M.R., Marshall, A.G.: Enhanced digestion efficiency, peptide ionization efficiency, and sequence resolution for protein hydrogen/deuterium exchange monitored by Fourier transform ion cyclotron resonance mass spectrometry. Anal. Chem. 80, 9034-9041 (2008)
28. Rey, M., Man, P., Brandolin, G., Forest, E., Pelosi, L.: Recombinant immobilized rhizopuspepsin as a new tool for protein digestion in hydrogen/deuterium exchange mass spectrometry. Rapid Commun. Mass Spectrom. 23, 3431-3438 (2009)