Structural analysis of a therapeutic monoclonal antibody dimer by hydroxyl radical footprinting

mAbs

Volumn 5, Issue 1, 2013, Pages 86-101

  Deperalta, Galahad ^a   Alvarez, Melissa ^a   Bechtel, Charity ^a   Dong, Ken ^a   McDonald, Ross ^a   Ling, Victor ^a  

^a GENENTECH INC   (United States)

Author keywords

Aggregates; Dimer; Fab 2 to Fab 2; Fab to Fab; FabRICATOR ; Higher order structure; Hydroxyl radical footprinting; IgG1; Mass spectrometry; Oxidative footprinting; Protein conformation; Radiolysis; Size exclusion chromatography (SEC); Synchrotron irradiation; Therapeutic mAb; UHPLCMS

Indexed keywords

DIMER; HYDROXYL RADICAL; IMMUNOGLOBULIN G1; MONOCLONAL ANTIBODY;

ANIMAL CELL; AQUEOUS SOLUTION; ARTICLE; CELL PROLIFERATION; CONTROLLED STUDY; DIMERIZATION; GEL PERMEATION CHROMATOGRAPHY; HUMAN; HUMAN CELL; HYDROXYL RADICAL FOOTPRINTING; IONIZATION; NONHUMAN; OXIDATION; PROTEIN DOMAIN; STRUCTURE ANALYSIS; SYNCHROTRON RADIATION;

ANTIBODIES, MONOCLONAL; CHROMATOGRAPHY, GEL; DIMERIZATION; HYDROXYL RADICAL; IMMUNOGLOBULIN FAB FRAGMENTS; IMMUNOGLOBULIN G; MASS SPECTROMETRY; MODELS, MOLECULAR; PEPTIDES; PROTEIN CONFORMATION; PROTEIN FOOTPRINTING; SYNCHROTRONS;

EID: 84872848394     PISSN: 19420862     EISSN: 19420870     Source Type: Journal    
DOI: 10.4161/mabs.22964     Document Type: Article

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