Recent progresses in gene delivery-based bone tissue engineering

Biotechnology Advances

Volumn 31, Issue 8, 2013, Pages 1695-1706

  Lu, Chia Hsin ^a   Chang, Yu Han ^b,c   Lin, Shih Yeh ^a   Li, Kuei Chang ^a   Hu, Yu Chen ^a  

^a NATIONAL TSING HUA UNIVERSITY   (Taiwan)

^b CHANG GUNG UNIVERSITY COLLEGE OF MEDICINE   (Taiwan)

^c CHANG GUNG MEMORIAL HOSPITAL   (Taiwan)

Author keywords

Bone; Gene delivery approach; Gene therapy; Regenerative medicine; Stem cell; Tissue engineering; Viral vectors

Indexed keywords

BONE; CELL ENGINEERING; GENE TRANSFER; REGENERATIVE MEDICINE; STEM CELLS; TISSUE; TISSUE ENGINEERING;

BONE ENGINEERING; BONE REPAIR; BONE TISSUE ENGINEERING; GENE DELIVERY; NONVIRAL VECTORS; RECENT PROGRESS; THERAPEUTIC GENES; VIRAL VECTORS;

GENE THERAPY;

BONE; GENE; GENETIC ENGINEERING; MEDICINE; STEM;

BONE MORPHOGENETIC PROTEIN; BONE MORPHOGENETIC PROTEIN 2; DEMINERALIZED BONE MATRIX; FLIPPASE; INTERLEUKIN 6; MICRORNA; MITOBRONITOL; OSTEOCLAST DIFFERENTIATION FACTOR; PLATELET DERIVED GROWTH FACTOR; SMALL INTERFERING RNA; TARTRATE-RESISTANT ACID PHOSPHATASE; TOLL LIKE RECEPTOR 3; VASCULOTROPIN; VIRUS VECTOR;

AAV; ACMNPV; ADBMP2; ADENO-ASSOCIATED VIRUS; ADENOVIRUS EXPRESSING BMP-2; ADIPOSE-DERIVED STEM CELLS; ANIMAL; ASCS; AUTOGRAPHA CALIFORNICA MULTIPLE NUCLEOPOLYHEDROVIRUS; BMP; BMP-2; BMSCS; BONE; BONE MARROW-DERIVED MESENCHYMAL STEM CELLS; COMPUTER ASSISTED TOMOGRAPHY; COX-2; CT; CXC CHEMOKINE RECEPTOR-4; CXCR4; CYCLOXYGENASE-2; EC; ECM; ENDOTHELIAL PROGENITOR CELL; ENDOTHELIUM CELL; EPCS; EXTRACELLULAR MATRIX; FGF-2; FIBROBLAST GROWTH FACTOR-2; FLIPPASE RECOGNITION TARGET; FLP; FRT; GAM; GENE ACTIVATED MATRIX; GENE DELIVERY APPROACH; GENE THERAPY; GENE TRANSFER; GENE VECTOR; HIF-1Α; HUMAN; HYPOXIA-INDUCIBLE FACTOR 1Α; I.V.; IFN-Γ; IL-6; INTERFERON-Γ; INTRAVENOUS DRUG ADMINISTRATION; IPSCS; LLP2A; MDSCS; MESENCHYMAL STEM CELL; MOUSE; MSCS; MUSCLE-DERIVED STEM CELLS; NEW ZEALAND WHITE; NZW; PEPTIDOMIMETIC LIGAND; PET; PLURIPOTENT STEM CELL; POSITRON EMISSION TOMOGRAPHY; RECEPTOR ACTIVATOR OF NUCLEAR FACTOR ΚB LIGAND; REGENERATIVE MEDICINE; REVIEW; SATB2; SCA-1; SPECIAL AT-RICH SEQUENCE-BINDING PROTEIN 2; STEM CELL; STEM CELL ANTIGEN-1; TGF-Β; TISSUE ENGINEERING; TLR-3; TNF-Α; TRANSFORMING GROWTH FACTOR Β; TRAP; TUMOR NECROSIS FACTOR-Α; VIRUS;

AAV; ACMNPV; ADBMP2; ADENO-ASSOCIATED VIRUS; ADENOVIRUS EXPRESSING BMP-2; ADIPOSE-DERIVED STEM CELLS; ASCS; AUTOGRAPHA CALIFORNICA MULTIPLE NUCLEOPOLYHEDROVIRUS; BMP; BMP-2; BMSCS; BONE; BONE MARROW-DERIVED MESENCHYMAL STEM CELLS; BONE MORPHOGENETIC PROTEIN; BONE MORPHOGENETIC PROTEIN 2; COMPUTED TOMOGRAPHY; COX-2; CT; CXC CHEMOKINE RECEPTOR-4; CXCR4; CYCLOXYGENASE-2; DBM; DEMINERALIZED BONE MATRIX; EC; ECM; ENDOTHELIAL CELLS; ENDOTHELIAL PROGENITOR CELLS; EPCS; EXTRACELLULAR MATRIX; FGF-2; FIBROBLAST GROWTH FACTOR-2; FLIPPASE; FLIPPASE RECOGNITION TARGET; FLP; FRT; GAM; GENE ACTIVATED MATRIX; GENE DELIVERY APPROACH; GENE THERAPY; HIF-1Α; HYPOXIA-INDUCIBLE FACTOR 1Α; I.V.; IFN-Γ; IL-6; INDUCED PLURIPOTENT STEM CELLS; INTERFERON-Γ; INTERLEUKIN 6; INTRAVENOUS; IPSCS; LLP2A; MDSCS; MESENCHYMAL STEM CELLS; MICRORNA; MIRNA; MSCS; MUSCLE-DERIVED STEM CELLS; NEW ZEALAND WHITE; NZW; PDGF; PEPTIDOMIMETIC LIGAND; PET; PLATELET-DERIVED GROWTH FACTOR; POSITRON EMISSION TOMOGRAPHY; RANKL; RECEPTOR ACTIVATOR OF NUCLEAR FACTOR ΚB LIGAND; REGENERATIVE MEDICINE; SATB2; SCA-1; SIRNA; SMALL INTERFERING RNA; SPECIAL AT-RICH SEQUENCE-BINDING PROTEIN 2; STEM CELL; STEM CELL ANTIGEN-1; TARTRATE-RESISTANT ACID PHOSPHATASE; TGF-Β; TISSUE ENGINEERING; TLR-3; TNF-Α; TOLL-LIKE RECEPTOR 3; TRANSFORMING GROWTH FACTOR Β; TRAP; TUMOR NECROSIS FACTOR-Α; VASCULAR ENDOTHELIAL GROWTH FACTOR; VEGF; VIRAL VECTORS;

ANIMALS; BONE AND BONES; GENE TRANSFER TECHNIQUES; GENETIC THERAPY; GENETIC VECTORS; HUMANS; MICE; REGENERATIVE MEDICINE; TISSUE ENGINEERING; VIRUSES;

EID: 84888000546     PISSN: 07349750     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.biotechadv.2013.08.015     Document Type: Review

References (184)

1. Airenne K.J., Hu Y.-C., Kost T.A., Smith R.H., Kotin R.M., Ono C., et al. Baculovirus: an insect-derived vector for diverse gene transfer applications. Mol Ther 2013, 21:739-749.
2. Alden T.D., Beres E.J., Laurent J.S., Engh J.A., Das S., London S.D., et al. The use of bone morphogenetic protein gene therapy in craniofacial bone repair. J Craniofac Surg 2000, 11:24-30.
3. Aslan H., Zilberman Y., Arbeli V., Sheyn D., Matan Y., Liebergall M., et al. Nucleofection-based ex vivo nonviral gene delivery to human stem cells as a platform for tissue regeneration. Tissue Eng 2006, 12:877-889.
4. Backstrom K.C., Bertone A.L., Wisner E.R., Weisbrode S.E. Response of induced bone defects in horses to collagen matrix containing the human parathyroid hormone gene. Am J Vet Res 2004, 65:1223-1232.
5. Bak X.Y., Lam D.H., Yang J.Y., Ye K., Wei E.L.X., Lim S.K., et al. Human embryonic stem cell-derived mesenchymal stem cells as cellular delivery vehicles for prodrug gene therapy of glioblastoma. Hum Gene Ther 2011, 22:1365-1377.
6. Baltzer A.W.A., Lattermann C., Whalen J.D., Wooley P., Weiss K., Grimm M., et al. Genetic enhancement of fracture repair: healing of an experimental segmental defect by adenoviral transfer of the BMP-2 gene. Gene Ther 2000, 7:734-739.
7. Banasik M.B., McCray P.B. Integrase-defective lentiviral vectors: progress and applications. Gene Ther 2009, 17:150-157.
8. Ben Arav A., Pelled G., Zilberman Y., Kimelman-Bleich N., Gazit Z., Schwarz E.M., et al. Adeno-associated virus-coated allografts: a novel approach for cranioplasty. J Tissue Eng Regen Med 2012, 6:e43-e50.
9. Bertone A.L., Pittman D.D., Bouxsein M.L., Li J., Clancy B., Seeherman H.J. Adenoviral-mediated transfer of human BMP-6 gene accelerates healing in a rabbit ulnar osteotomy model. J Orthop Res 2004, 22:1261-1270.
10. Betz O.B., Betz V.M., Nazarian A., Pilapil C.G., Vrahas M.S., Bouxsein M.L., et al. Direct percutaneous gene delivery to enhance healing of segmental bone defects. J Bone Joint Surg Am 2006, 88A:355-365.
11. Betz O.B., Betz V.M., Nazarian A., Egermann M., Gerstenfeld L.C., Einhorn T.A., et al. Delayed administration of adenoviral BMP-2 vector improves the formation of bone in osseous defects. Gene Ther 2007, 14:1039-1044.
12. Betz V.M., Betz O.B., Glatt V., Gerstenfeld L.C., Einhorn T.A., Bouxsein M.L., et al. Healing of segmental bone defects by direct percutaneous gene delivery: effect of vector dose. Hum Gene Ther 2007, 18:907-915.
13. Bilousova G., Jun D.H., King K.B., De Langhe S., Chick W.S., Torchia E.C., et al. Osteoblasts derived from induced pluripotent stem cells form calcified structures in scaffolds both in vitro and in vivo. Stem Cells 2011, 29:206-216.
14. Blum J.S., Barry M.A., Mikos A.G., Jansen J.A. In vivo evaluation of gene therapy vectors in ex vivo-derived marrow stromal cells for bone regeneration in a rat critical-size calvarial defect model. Hum Gene Ther 2003, 14:1689-1701.
15. Boden S.D. The ABCs of BMPs. Orthop Nurs 2005, 24:49-52.
16. Bonadio J., Smiley E., Patil P., Goldstein S. Localized, direct plasmid gene delivery in vivo: prolonged therapy results in reproducible tissue regeneration. Nat Med 1999, 5:753-759.
17. Bose S., Roy M., Bandyopadhyay A. Recent advances in bone tissue engineering scaffolds. Trends Biotechnol 2012, 30:546-554.
18. Bright C., Park Y.S., Sieber A.N., Kostuik J.P., Leong K.W. In vivo evaluation of plasmid DNA encoding OP-1 protein for spine fusion. Spine (Phila Pa 1976) 2006, 31:2163-2172.
19. Buckwalter J.A., Einhorn T.A., Bolander M.E., Cruess R.L. Healing of the musculoskeletal tissues. Rockwood and Green's fractures in adults 1996, 261-304. Lippincott Williams & Wilkins. 4th ed. R.W. Bucholz, J.D. Heckman, C. Court-Brown, P. Tornetta, K.J. Koval, M.A. Wirth (Eds.).
20. Bueno E.M., Glowacki J. Cell-free and cell-based approaches for bone regeneration. Nat Rev Rheumatol 2009, 5:685-697.
21. Byers B.A., Guldberg R.E., Hutmacher D.W., Garcia A.J. Effects of Runx2 genetic engineering and in vitro maturation of tissue-engineered constructs on the repair of critical size bone defects. J Biomed Mater Res A 2006, 76:646-655.
22. Cartier N., Hacein-Bey-Abina S., Bartholomae C.C., Veres G., Schmidt M., Kutschera I., et al. Hematopoietic stem cell gene therapy with a lentiviral vector in X-linked adrenoleukodystrophy. Science 2009, 326:818-823.
23. Chang P.C., Cirelli J.A., Jin Q., Seol Y.J., Sugai J.V., D'Silva N.J., et al. Adenovirus encoding human platelet-derived growth factor-B delivered to alveolar bone defects exhibits safety and biodistribution profiles favorable for clinical use. Hum Gene Ther 2009, 20:486-496.
24. Chen H.-C., Lee H.-P., Ho Y.-C., Sung M.-L., Hu Y.-C. Combination of baculovirus-mediated gene transfer and rotating-shaft bioreactor for cartilage tissue engineering. Biomaterials 2006, 27:3154-3162.
25. Chen G.-Y., Shiah H.-C., Su H.-J., Chen C.-Y., Chuang Y.-J., Lo W.-H., et al. Baculovirus transduction of mesenchymal stem cells triggers the toll-like receptor 3 (TLR3) pathway. J Virol 2009, 83:10548-10556.
26. Chen H.-C., Chang Y.-H., Chuang C.-K., Lin C.-Y., Sung L.-Y., Wang Y.-H., et al. The repair of osteochondral defects using baculovirus-mediated gene transfer with de-differentiated chondrocytes in bioreactor culture. Biomaterials 2009, 30:674-681.
27. Chen C.-Y., Wu H.-H., Chen C.-P., Chern S.-R., Hwang S.-M., Huang S.-F., et al. Biosafety assessment of human mesenchymal stem cells engineered by hybrid baculovirus vectors. Mol Pharm 2011, 8:1505-1514.
28. Chen F., Zhang X., Sun S., Zara J.N., Zou X., Chiu R., et al. NELL-1, an osteoinductive factor, is a direct transcriptional target of Osterix. PLoS One 2011, 6:e24638.
29. Chen G.-Y., Pang D.W.P., Hwang S.-M., Tuan H.-Y., Hu Y.-C. A graphene-based platform for induced pluripotent stem cells culture and differentiation. Biomaterials 2012, 33:418-427.
30. Chew S.A., Kretlow J.D., Spicer P.P., Edwards A.W., Baggett L.S., Tabata Y., et al. Delivery of plasmid DNA encoding bone morphogenetic protein-2 with a biodegradable branched polycationic polymer in a critical-size rat cranial defect model. Tissue Eng Part A 2011, 17:751-763.
31. Chick H.E., Nowrouzi A., Fronza R., McDonald R.A., Kane N.M., Alba R., et al. Integrase-deficient lentiviral vectors mediate efficient gene transfer to human vascular smooth muscle cells with minimal genotoxic risk. Hum Gene Ther 2012, 23:1247-1257.
32. Cho S.W., Sun H.J., Yang J.-Y., Jung J.Y., An J.H., Cho H.Y., et al. Transplantation of mesenchymal stem cells overexpressing RANK-Fc or CXCR4 prevents bone loss in ovariectomized mice. Mol Ther 2009, 17:1979-1987.
33. Chuang C.-K., Sung L.-Y., Hwang S.-M., Lo W.-H., Chen H.-C., Hu Y.-C. Baculovirus as a new gene delivery vector for stem cells engineering and bone tissue engineering. Gene Ther 2007, 14:1417-1424.
34. Chuang C.-K., Wong T.-H., Hwang S.-M., Chang Y.-H., Chen Y.-H., Chiu Y.-C., et al. Baculovirus transduction of mesenchymal stem cells: in vitro responses and in vivo immune responses after cell transplantation. Mol Ther 2009, 17:889-896.
35. Chuang C.-K., Lin K.-J., Lin C.-Y., Chang Y.-H., Yen T.-C., Hwang S.-M., et al. Xenotransplantation of human mesenchymal stem cells into immunocompetent rats for calvarial bone repair. Tissue Eng Part A 2010, 16:479-488.
36. Colburn N.T., Zaal K.J.M., Wang F., Tuan R.S. A role for γ/δ T cells in a mouse model of fracture healing. Arthritis Rheum 2009, 60:1694-1703.
37. De Biase P., Capanna R. Clinical applications of BMPs. Injury 2005, 36(Suppl. 3):S43-S46.
38. Edwards P.C., Ruggiero S., Fantasia J., Burakoff R., Moorji S.M., Paric E., et al. Sonic hedgehog gene-enhanced tissue engineering for bone regeneration. Gene Ther 2005, 12:75-86.
39. Egermann M., Lill C.A., Griesbeck K., Evans C.H., Robbins P.D., Schneider E., et al. Effect of BMP-2 gene transfer on bone healing in sheep. Gene Ther 2006, 13:1290-1299.
40. Endo M., Kuroda S., Kondo H., Maruoka Y., Ohya K., Kasugai S. Bone regeneration by modified gene-activated matrix: effectiveness in segmental tibial defects in rats. Tissue Eng 2006, 12:489-497.
41. Evans C.H. Gene delivery to bone. Adv Drug Deliv Rev 2012, 64:1331-1340.
42. Evans C.H., Gouze E., Gouze J.N., Robbins P.D., Ghivizzani S.C. Gene therapeutic approaches-transfer in vivo. Adv Drug Deliv Rev 2006, 58:243-258.
43. Evans C.H., Ghivizzani S.C., Robbins P.D. Arthritis gene therapy's first death. Arthritis Res Ther 2008, 10.
44. Evans C.H., Ghivizzani S.C., Robbins P.D. Progress and prospects: genetic treatments for disorders of bones and joints. Gene Ther 2009, 16:944-952.
45. Evans C.H., Ghivizzani S.C., Robbins P.D. Orthopedic gene therapy in 2008. Mol Ther 2009, 17:231-244.
46. Evans C.H., Liu F.J., Glatt V., Hoyland J.A., Kirker-Head C., Walsh A., et al. Use of genetically modified muscle and fat grafts to repair defects in bone and cartilage. Eur Cell Mater 2009, 18:96-111.
47. Fang J., Zhu Y.Y., Smiley E., Bonadio J., Rouleau J.P., Goldstein S.A., et al. Stimulation of new bone formation by direct transfer of osteogenic plasmid genes. Proc Natl Acad Sci U S A 1996, 93:5753-5758.
48. Feeley B.T., Conduah A.H., Sugiyama O., Krenek L., Chen I.S.Y., Lieberman J.R. In vivo molecular imaging of adenoviral versus lentiviral gene therapy in two bone formation models. J Orthop Res 2006, 24:1709-1721.
49. Frank K.M., Hogarth D.K., Miller J.L., Mandal S., Mease P.J., Samulski R.J., et al. Brief report: investigation of the cause of death in a gene-therapy trial. N Engl J Med 2009, 361:161-169.
50. Gafni Y., Pelled G., Zilberman Y., Turgeman G., Apparailly F., Yotvat H., et al. Gene therapy platform for bone regeneration using an exogenously regulated, AAV-2-based gene expression system. Mol Ther 2004, 9:587-595.
51. Gafni Y., Turgeman G., Liebergal M., Pelled G., Gazit Z., Gazit D. Stem cells as vehicles for orthopedic gene therapy. Gene Ther 2004, 11:417-426.
52. Gamradt S.C., Lieberman J.R. Genetic modification of stem cells to enhance bone repair. Ann Biomed Eng 2004, 32:136-147.
53. Gao J., Dennis J.E., Muzic R.F., Lundberg M., Caplan A.I. The dynamic in vivo distribution of bone marrow-derived mesenchymal stem cells after infusion. Cells Tissues Organs 2001, 169:12-20.
54. Garty S., Kimelman-Bleich N., Hayouka Z., Cohn D., Friedler A., Pelled G., et al. Peptide-modified "smart" hydrogels and genetically engineered stem cells for skeletal tissue engineering. Biomacromolecules 2010, 11:1516-1526.
55. Geiger F., Bertram H., Berger I., Lorenz H., Wall O., Eckhardt C., et al. Vascular endothelial growth factor gene-activated matrix (VEGF165-GAM) enhances osteogenesis and angiogenesis in large segmental bone defects. J Bone Miner Res 2005, 20:2028-2035.
56. Geuze R.E., Theyse L.F., Kempen D.H., Hazewinkel H.A., Kraak H.Y., Oner F.C., et al. A differential effect of bone morphogenetic protein-2 and vascular endothelial growth factor release timing on osteogenesis at ectopic and orthotopic sites in a large-animal model. Tissue Eng Part A 2012, 18:2052-2062.
57. Granero-Molto F., Weis J.A., Miga M.I., Landis B., Myers T.J., O'Rear L., et al. Regenerative effects of transplanted mesenchymal stem cells in fracture healing. Stem Cells 2009, 27:1887-1898.
58. Guan M., Yao W., Liu R., Lam K.S., Nolta J., Jia J., et al. Directing mesenchymal stem cells to bone to augment bone formation and increase bone mass. Nat Med 2012, 18:456-462.
59. Gysin R., Wergedal J.E., Sheng M.H., Kasukawa Y., Miyakoshi N., Chen S.T., et al. Ex vivo gene therapy with stromal cells transduced with a retroviral vector containing the BMP4 gene completely heals critical size calvarial defect in rats. Gene Ther 2002, 9:991-999.
60. Hacein-Bey-Abina S., Von Kalle C., Schmidt M., McCcormack M.P., Wulffraat N., Leboulch P., et al. LMO2-associated clonal T cell proliferation in two patients after gene therapy for SCID-X1. Science 2003, 302:415-419.
61. Hacein-Bey-Abina S., Garrigue A., Wang G.P., Soulier J., Lim A., Morillon E., et al. Insertional oncogenesis in 4 patients after retrovirus-mediated gene therapy of SCID-X1. J Clin Invest 2008, 118:3132-3142.
62. Hacein-Bey-Abina S., Hauer J., Lim A., Picard C., Wang G.P., Berry C.C., et al. Efficacy of gene therapy for X-linked severe combined immunodeficiency. New Engl J Med 2010, 363:355-364.
63. Hall S.L., Lau K.-H.W., Chen S.-T., Wergedal J.E., Srivastava A., Klamut H., et al. Sca-1+ hematopoietic cell-based gene therapy with a modified FGF-2 increased endosteal/trabecular bone formation in mice. Mol Ther 2007, 15:1881-1889.
64. He X., Dziak R., Yuan X., Mao K., Genco R., Swihart M., et al. BMP2 genetically engineered MSCs and EPCs promote vascularized bone regeneration in rat critical-sized calvarial bone defects. PLoS One 2013, 8:e60473.
65. Heckl D., Schwarzer A., Haemmerle R., Steinemann D., Rudolph C., Skawran B., et al. Lentiviral vector induced insertional haploinsufficiency of Ebf1 causes murine leukemia. Mol Ther 2012, 20:1187-1195.
66. Ho Y.-C., Lee H.-P., Hwang S.-M., Lo W.-H., Chen H.-C., Chung C.-K., et al. Baculovirus transduction of human mesenchymal stem cell-derived progenitor cells: variation of transgene expression with cellular differentiation states. Gene Ther 2006, 13:1471-1479.
67. Hollinger J.O., Kleinschmidt J.C. The critical size defect as an experimental model to test bone repair materials. J Craniofac Surg 1990, 1:60-68.
68. Hsu W.K., Sugiyama O., Park S.H., Conduah A., Feeley B.T., Liu N.Q., et al. Lentiviral-mediated BMP-2 gene transfer enhances healing of segmental femoral defects in rats. Bone 2007, 40:931-938.
69. Hu Y.-C. Baculovirus vectors for gene therapy. Adv Virus Res 2006, 68:287-320.
70. Hu Y.-C. Baculoviral vectors for gene delivery: a review. Curr Gene Ther 2008, 8:54-65.
71. Huang S., Kamihira M. Development of hybrid viral vectors for gene therapy. Biotechnol Adv 2013, 31:208-223.
72. Huang Y.C., Simmons C., Kaigler D., Rice K.G., Mooney D.J. Bone regeneration in a rat cranial defect with delivery of PEI-condensed plasmid DNA encoding for bone morphogenetic protein-4 (BMP-4). Gene Ther 2005, 12:418-426.
73. Ishihara A., Shields K.M., Litsky A.S., Mattoon J.S., Weisbrode S.E., Bartlett J.S., et al. Osteogenic gene regulation and relative acceleration of healing by adenoviral-mediated transfer of human BMP-2 or -6 in equine osteotomy and ostectomy models. J Orthop Res 2008, 26:764-771.
74. Itaka K., Ohba S., Miyata K., Kawaguchi H., Nakamura K., Takato T., et al. Bone regeneration by regulated in vivo gene transfer using biocompatible polyplex nanomicelles. Mol Ther 2007, 15:1655-1662.
75. Ito H., Koefoed M., Tiyapatanaputi P., Gromov K., Goater J.J., Carmouche J., et al. Remodeling of cortical bone allografts mediated by adherent rAAV-RANKL and VEGF gene therapy. Nat Med 2005, 11:291-297.
76. Jabbarzadeh E., Starnes T., Khan Y.M., Jiang T., Wirtel A.J., Deng M., et al. Induction of angiogenesis in tissue-engineered scaffolds designed for bone repair: a combined gene therapy-cell transplantation approach. Proc Natl Acad Sci 2008, 105:11099-11104.
77. Jane J.A., Dunford B.A., Kron A., Pittman D.D., Sasaki T., Li J.Z., et al. Ectopic osteogenesis using adenoviral bone morphogenetic protein (BMP)-4 and BMP-6 gene transfer. Mol Ther 2002, 6:464-470.
78. Ke J., Zheng L.W., Cheung L.K. Orthopaedic gene therapy using recombinant adeno-associated virus vectors. Arch Oral Biol 2011, 56:619-628.
79. Keeney M., van den Beucken J.J., van der Kraan P.M., Jansen J.A., Pandit A. The ability of a collagen/calcium phosphate scaffold to act as its own vector for gene delivery and to promote bone formation via transfection with VEGF(165). Biomaterials 2010, 31:2893-2902.
80. Kempen D.H.R., Lu L., Heijink A., Hefferan T.E., Creemers L.B., Maran A., et al. Effect of local sequential VEGF and BMP-2 delivery on ectopic and orthotopic bone regeneration. Biomaterials 2009, 30:2816-2825.
81. Kimelman Bleich N., Kallai I., Lieberman J.R., Schwarz E.M., Pelled G., Gazit D. Gene therapy approaches to regenerating bone. Adv Drug Deliv Rev 2012, 64:1320-1330.
82. Kimelman N., Pelled G., Helm G.A., Huard J., Schwarz E.M., Gazit D. Review: gene- and stem cell-based therapeutics for bone regeneration and repair. Tissue Eng 2007, 13:1135-1150.
83. Kimelman-Bleich N., Pelled G., Sheyn D., Kallai I., Zilberman Y., Mizrahi O., et al. The use of a synthetic oxygen carrier-enriched hydrogel to enhance mesenchymal stem cell-based bone formation in vivo. Biomaterials 2009, 30:4639-4648.
84. Kimelman-Bleich N., Pelled G., Zilberman Y., Kallai I., Mizrahi O., Tawackoli W., et al. Targeted gene-and-host progenitor cell therapy for nonunion bone fracture repair. Mol Ther 2011, 19:53-59.
85. Kneser U., Schaefer D.J., Polykandriotis E., Horch R.E. Tissue engineering of bone: the reconstructive surgeon's point of view. J Cell Mol Med 2006, 10:7-19.
86. Kochanek S., Clemens P.R., Mitani K., Chen H.H., Chan S., Caskey C.T. A new adenoviral vector: replacement of all viral coding sequences with 28kb of DNA independently expressing both full-length dystrophin and beta-galactosidase. Proc Natl Acad Sci U S A 1996, 93:5731-5736.
87. Koefoed M., Ito H., Gromov K., Reynolds D.G., Awad H.A., Rubery P.T., et al. Biological effects of rAAV-caAlk2 coating on structural allograft healing. Mol Ther 2005, 12:212-218.
88. Kofron M.D., Laurencin C.T. Bone tissue engineering by gene delivery. Adv Drug Deliv Rev 2006, 58:555-576.
89. Kost T.A., Condreay J.P., Jarvis D.L. Baculovirus as versatile vectors for protein expression in insect and mammalian cells. Nat Biotechnol 2005, 23:567-575.
90. Kumar S., Wan C., Ramaswamy G., Clemens T.L., Ponnazhagan S. Mesenchymal stem cells expressing osteogenic and angiogenic factors synergistically enhance bone formation in a mouse model of segmental bone defect. Mol Ther 2010, 18:1026-1034.
91. Lattanzi W., Parrilla C., Fetoni A., Logroscino G., Straface G., Pecorini G., et al. Ex vivo-transduced autologous skin fibroblasts expressing human Lim mineralization protein-3 efficiently form new bone in animal models. Gene Ther 2008, 15:1330-1343.
92. Lee J.Y., Peng H.R., Usas A., Musgrave D., Cummins J., Pelinkovic D., et al. Enhancement of bone healing based on ex vivo gene therapy using human muscle-derived cells expressing bone morphogenetic protein 2. Hum Gene Ther 2002, 13:1201-1211.
93. Lee E.X., Lam D.H., Wu C., Yang J., Tham C.K., Ng W.H., et al. Glioma gene therapy using induced pluripotent stem cell derived neural stem cells. Mol Pharm 2011, 8:1515-1524.
94. Li Y., Fan L., Liu S., Liu W., Zhang H., Zhou T., et al. The promotion of bone regeneration through positive regulation of angiogenic-osteogenic coupling using microRNA-26a. Biomaterials 2013, 34:5048-5058.
95. Lian J.B., Stein G.S., van Wijnen A.J., Stein J.L., Hassan M.Q., Gaur T., et al. MicroRNA control of bone formation and homeostasis. Nat Rev Endocrinol 2012, 8:212-227.
96. Lieberman J.R., Le L.Q., Wu L., Finerman G.A.M., Berk A., Witte O.N., et al. Regional gene therapy with a BMP-2-producing murine stromal cell line induces heterotopic and orthotopic bone formation in rodents. J Orthop Res 1998, 16:330-339.
97. Lieberman J.R., Daluiski A., Stevenson S., Wu L., McAllister P., Lee Y.P., et al. The effect of regional gene therapy with bone morphogenetic protein-2-producing bone-marrow cells on the repair of segmental femoral defects in rats. J Bone Joint Surg Am 1999, 81A:905-917.
98. Lieberman J.R., Daluiski A., Einhorn T.A. The role of growth factors in the repair of bone. Biology and clinical applications. J Bone Joint Surg Am 2002, 84-A:1032-1044.
99. Lien C.Y., Chih-Yuan Ho K., Lee O.K., Blunn G.W., Su Y. Restoration of bone mass and strength in glucocorticoid-treated mice by systemic transplantation of CXCR4 and cbfa-1 co-expressing mesenchymal stem cells. J Bone Miner Res 2009, 24:837-848.
100. Lin C.-Y., Chang Y.-H., Lin K.-J., Yen T.-Z., Tai C.-L., Chen C.-Y., et al. The healing of critical-sized femoral segmental bone defects in rabbits using baculovirus-engineered mesenchymal stem cells. Biomaterials 2010, 31:3222-3230.
101. Lin C.-Y., Lu C.-H., Luo W.-Y., Chang Y.-H., Sung L.-Y., Chiu H.-Y., et al. Baculovirus as a gene delivery vector for cartilage and bone tissue engineering. Curr Gene Ther 2010, 10:242-254.
102. Lin C.-Y., Lin K.-J., Kao C.-Y., Chen M.-C., Yen T.-Z., Lo W.-H., et al. The role of adipose-derived stem cells engineered with the persistently expressing hybrid baculovirus in the healing of massive bone defects. Biomaterials 2011, 32:6505-6514.
103. Lin L., Shen Q., Leng H., Duan X., Fu X., Yu C. Synergistic inhibition of endochondral bone formation by silencing HIF-1α and Runx2 in trauma-induced heterotopic ossification. Mol Ther 2011, 19:1426-1432.
104. Lin C.-Y., Chang Y.-H., Kao C.-Y., Lu C.-H., Sung L.-Y., Yen T.-C., et al. Augmented healing of critical-size calvarial defects by baculovirus-engineered MSCs that persistently express growth factors. Biomaterials 2012, 33:3682-3692.
105. Lin C.-Y., Lin K.-J., Li K.-C., Sung L.-Y., Hsueh S., Lu C.-H., et al. Immune responses during healing of massive segmental femoral bone defects mediated by hybrid baculovirus-engineered ASCs. Biomaterials 2012, 33:7422-7434.
106. Liu C.Y.-Y., Chen H.-Z., Chao Y.-C. Maximizing baculovirus-mediated foreign proteins expression in mammalian cells. Curr Gene Ther 2010, 10:232-241.
107. Liu Y.G., Zhou Y., Hu X., Fu J.J., Pan Y., Chu T.W. Effect of vascular endothelial growth factor 121 adenovirus transduction in rabbit model of femur head necrosis. J Trauma 2011, 70:1519-1523.
108. Liu F., Porter R.M., Wells J., Glatt V., Pilapil C., Evans C.H. Evaluation of BMP-2 gene-activated muscle grafts for cranial defect repair. J Orthop Res 2012, 30:1095-1102.
109. Liu Y., Wang L., Kikuiri T., Akiyama K., Chen C., Xu X., et al. Mesenchymal stem cell-based tissue regeneration is governed by recipient T lymphocytes via IFN-γ and TNF-α. Nat Med 2012, 17:1594-1601.
110. Lo W.-H., Hwang S.-M., Chuang C.-K., Chen C.-Y., Hu Y.-C. Development of a hybrid baculoviral vector for sustained transgene expression. Mol Ther 2009, 17:658-666.
111. Lu S.S., Zhang X., Soo C., Hsu T., Napoli A., Aghaloo T., et al. The osteoinductive properties of Nell-1 in a rat spinal fusion model. Spine J 2007, 7:50-60.
112. Lu C.-H., Lin K.-J., Chiu H.-Y., Chen C.-Y., Yen T.-C., Hwang S.-M., et al. Improved chondrogenesis and engineered cartilage formation from TGF-β3-expressing adipose-derived stem cells cultured in the rotating-shaft bioreactor. Tissue Eng Part A 2012, 18:2114-2124.
113. Lu C.-H., Yeh T.-S., Yeh C.-L., Fang Y.-H.D., Sung L.-Y., Lin S.-Y., et al. Regenerating cartilages by engineered ASCs: prolonged TGF-β3/BMP-6 expression improved articular cartilage formation and restored zonal structure. Mol Ther 2013, [Epub ahead of print]. 10.1038/mt.2013.165.
114. Luo T., Zhang W., Shi B., Cheng X., Zhang Y. Enhanced bone regeneration around dental implant with bone morphogenetic protein 2 gene and vascular endothelial growth factor protein delivery. Clin Oral Implants Res 2012, 23:467-473.
115. Marino J.T., Ziran B.H. Use of solid and cancellous autologous bone graft for fractures and nonunions. Orthop Clin North Am 2010, 41:15-26.
116. Marshall E. Gene therapy death prompts review of adenovirus vector. Science 1999, 286:2244-2245.
117. McCarty D.M. Self-complementary AAV, vectors; advances and applications. Mol Ther 2008, 16:1648-1656.
118. Mease P.J., Wei N., Fudman E.J., Kivitz A.J., Schechtman J., Trapp R.G., et al. Safety, tolerability, and clinical outcomes after intraarticular injection of a recombinant adeno-associated vector containing a tumor necrosis factor antagonist gene: results of a phase 1/2 study. J Rheumatol 2010, 37:692-703.
119. Merrihew R.V., Clay W.C., Condreay J.P., Witherspoon S.M., Dallas W.S., Kost T.A. Chromosomal integration of transduced recombinant baculovirus DNA in mammalian cells. J Virol 2001, 75:903-909.
120. Mingozzi F., High K.A. Immune responses to AAV in clinical trials. Curr Gene Ther 2011, 11:321-330.
121. Mizrahi O., Sheyn D., Tawackoli W., Kallai I., Oh A., Su S., et al. BMP-6 is more efficient in bone formation than BMP-2 when overexpressed in mesenchymal stem cells. Gene Ther 2013, 20:370-377.
122. Moutsatsos I.K., Turgeman G., Zhou S.H., Kurkalli B.G., Pelled G., Tzur L., et al. Exogenously regulated stem cell-mediated gene therapy for bone regeneration. Mol Ther 2001, 3:449-461.
123. Nayak S., Herzog R.W. Progress and prospects: immune responses to viral vectors. Gene Ther 2010, 17:295-304.
124. Niemeyer P., Fechner K., Milz S., Richter W., Suedkamp N.P., Mehlhorn A.T., et al. Comparison of mesenchymal stem cells from bone marrow and adipose tissue for bone regeneration in a critical size defect of the sheep tibia and the influence of platelet-rich plasma. Biomaterials 2010, 31:3572-3579.
125. Noel D., Gazit D., Bouquet C., Apparailly F., Bony C., Plence P., et al. Short-term BMP-2 expression is sufficient for in vivo osteochondral differentiation of mesenchymal stem cells. Stem Cells 2004, 22:74-85.
126. Park J., Ries J., Gelse K., Kloss F., von der Mark K., Wiltfang J., et al. Bone regeneration in critical size defects by cell-mediated BMP-2 gene transfer: a comparison of adenoviral vectors and liposomes. Gene Ther 2003, 10:1089-1098.
127. Park J.S., Na K., Woo D.G., Yang H.N., Kim J.M., Kim J.H., et al. Non-viral gene delivery of DNA polyplexed with nanoparticles transfected into human mesenchymal stem cells. Biomaterials 2010, 31:124-132.
128. Patel Z.S., Young S., Tabata Y., Jansen J.A., Wong M.E., Mikos A.G. Dual delivery of an angiogenic and an osteogenic growth factor for bone regeneration in a critical size defect model. Bone 2008, 43:931-940.
129. Pelled G., Ben-Arav A., Hock C., Reynolds D.G., Yazici C., Zilberman Y., et al. Direct gene therapy for bone regeneration: gene delivery, animal models, and outcome measures. Tissue Eng Part B Rev 2010, 16:13-20.
130. Peng H., Wright V., Usas A., Gearhart B., Shen H.C., Cummins J., et al. Synergistic enhancement of bone formation and healing by stem cell-expressed VEGF and bone morphogenetic protein-4. J Clin Invest 2002, 110:751-759.
131. Peng H., Usas A., Gearhart B., Olshanski A., Shen H.C., Huard J. Converse relationship between in vitro osteogenic differentiation and in vivo bone healing elicited by different populations of muscle-derived cells genetically engineered to express BMP4. J Bone Miner Res 2004, 19:630-641.
132. Peng H., Usas A., Hannallah D., Olshanski A., Cooper G.M., Huard J. Noggin improves bone healing elicited by muscle stem cells expressing inducible BMP4. Mol Ther 2005, 12:239-246.
133. Peng H., Usas A., Olshanski A., Ho A.M., Gearhart B., Cooper G.M., et al. VEGF improves, whereas sFlt1 inhibits, BMP-2 induced bone formation and bone healing through modulation of angiogenesis. J Bone Miner Res 2005, 20:2017-2027.
134. Peterson B., Zhang J., Iglesias R., Kabo M., Hedrick M., Benhaim P., et al. Healing of critically sized femoral defects, using genetically modified mesenchymal stem cells from human adipose tissue. Tissue Eng 2005, 11:120-129.
135. Phillips J.E., Gersbach C.A., Garcia A.J. Virus-based gene therapy strategies for bone regeneration. Biomaterials 2007, 28:211-229.
136. Phinney D.G., Prockop D.J. Concise review: mesenchymal stem/multipotent stromal cells: the state of transdifferentiation and modes of tissue repair-current views. Stem Cells 2007, 25:2896-2902.
137. Musculoskeletal conditions in the United States 1992, American Academy of Orthopaedic Surgeons, Rosemont, IL. A. Praemer, S. Furner, D. Rice (Eds.).
138. Prockop D.J. Repair of tissues by adult stem/progenitor cells (MSCs): controversies, myths, and changing paradigms. Mol Ther 2009, 17:939-946.
139. Reinke S., Geissler S., Taylor W.R., Schmidt-Bleek K., Juelke K., Schwachmeyer V., et al. Terminally differentiated CD8+ T cells negatively affect bone regeneration in humans. Sci Transl Med 2013, 5:177ra36.
140. Ronen K., Negre O., Roth S., Colomb C., Malani N., Denaro M., et al. Distribution of lentiviral vector integration sites in mice following therapeutic gene transfer to treat beta-thalassemia. Mol Ther 2011, 19:1273-1286.
141. Rundle C.H., Miyakoshi N., Kasukawa Y., Chen S.T., Sheng M.H.C., Wergedal J.E., et al. In vivo bone formation in fracture repair induced by direct retroviral-based gene therapy with bone morphogenetic protein-4. Bone 2003, 32:591-601.
142. Rundle C.H., Strong D.D., Chen S.T., Linkhart T.A., Sheng M.H.C., Wergedal J.E., et al. Retroviral-based gene therapy with cyclooxygenase-2 promotes the union of bony callus tissues and accelerates fracture heating in the rat. J Gene Med 2008, 10:229-241.
143. Santo V.E., Gomes M.E., Mano J.F., Reis R.L. Controlled release strategies for bone, cartilage, and osteochondral engineering-part I: recapitulation of native tissue healing and variables for the design of delivery systems. Tissue Eng Part B Rev 2013, 19:308-326.
144. Santos M.I., Reis R.L. Vascularization in bone tissue engineering: physiology, current strategies, major hurdles and future challenges. Macromol Biosci 2010, 10:12-27.
145. Schambach A., Zychlinski D., Ehrnstroem B., Baum C. Biosafety features of lentiviral vectors. Hum Gene Ther 2013, 24:132-142.
146. Seymour L.W., Thrasher A.J. Gene therapy matures in the clinic. Nat Biotechnol 2012, 30:588-593.
147. Shegarfi H., Reikeras O. Bone transplantation and immune response. J Orthop Surg 2009, 17:206-211.
148. Sheyn D., Pelled G., Zilberman Y., Talasazan F., Frank J.M., Gazit D., et al. Nonvirally engineered porcine adipose tissue-derived stem cells: use in posterior spinal fusion. Stem Cells 2008, 26:1056-1064.
149. Sheyn D., Ruthemann M., Mizrahi O., Kallai I., Zilberman Y., Tawackoli W., et al. Genetically modified mesenchymal stem cells induce mechanically stable posterior spine fusion. Tissue Eng Part A 2010, 16:3679-3686.
150. Sheyn D., Kallai I., Tawackoli W., Yakubovich D.C., Oh A., Su S.S., et al. Gene-modified adult stem cells regenerate vertebral bone defect in a rat model. Mol Pharm 2011, 8:1592-1601.
151. Smith R.H., Levy J.R., Kotin R.M. A simplified baculovirus-AAV expression vector system coupled with one-step affinity purification yields high-titer rAAV stocks from insect cells. Mol Ther 2009, 17:1888-1896.
152. Smith J.O., Aarvold A., Tayton E.R., Dunlop D.G., Oreffo R.O. Skeletal tissue regeneration: current approaches, challenges, and novel reconstructive strategies for an aging population. Tissue Eng Part B Rev 2011, 17:307-320.
153. Steinhardt Y., Aslan H., Regev E., Zilberman Y., Kallai I., Gazit D., et al. Maxillofacial-derived stem cells regenerate critical mandibular bone defect. Tissue Eng Part A 2008, 14:1763-1773.
154. Street J., Bao M., deGuzman L., Bunting S., Peale F.V., Ferrara N., et al. Vascular endothelial growth factor stimulates bone repair by promoting angiogenesis and bone turnover. Proc Natl Acad Sci U S A 2002, 99:9656-9661.
155. Sung L.-Y., Lo W.-H., Chiu H.-Y., Chen H.-C., Chuang C.-K., Lee H.-P., et al. Modulation of chondrocyte phenotype via baculovirus-mediated growth factor expression. Biomaterials 2007, 28:3437-3447.
156. Sung L.-Y., Chen C.-L., Lin S.-Y., Hwang S.-M., Li K.-C., Lan A.S.-M., et al. Enhanced and prolonged baculovirus-mediated expression by incorporating recombinase system and in cis elements: a comparative study. Nucleic Acids Res 2013, [Epub ahead of print]. 10.1093/nar/gkt442.
157. Szpalski C., Barr J., Wetterau M., Saadeh P.B., Warren S.M. Cranial bone defects: current and future strategies. Neurosurg Focus 2010, 29:E8.
158. Tashiro K., Inamura M., Kawabata K., Sakurai F., Yamanishi K., Hayakawa T., et al. Efficient adipocyte and osteoblast differentiation from mouse induced pluripotent stem cells by adenoviral transduction. Stem Cells 2009, 27:1802-1811.
159. Tseng S.S., Lee M.A., Reddi H. Nonunions and the potential of stem cells in fracture-healing. J Bone Joint Surg Am 2008, 90A:92-98.
160. Turgeman G., Pittman D.D., Muller R., Kurkalli B.G., Zhou S.H., Pelled G., et al. Engineered human mesenchymal stem cells: a novel platform for skeletal cell mediated gene therapy. J Gene Med 2001, 3:240-251.
161. Verma I.M., Weitzman M.D. Gene therapy: twenty-first century medicine. Annu Rev Biochem 2005, 74:711-738.
162. Villavicencio A.T., Burneikiene S., Nelson E.L., Bulsara K.R., Favors M., Thramann J. Safety of transforaminal lumbar interbody fusion and intervertebral recombinant human bone morphogenetic protein-2. J Neurosurg Spine 2005, 3:436-443.
163. Virk M.S., Conduah A., Park S.H., Liu N., Sugiyama O., Cuomo A., et al. Influence of short-term adenoviral vector and prolonged lentiviral vector mediated bone morphogenetic protein-2 expression on the quality of bone repair in a rat femoral defect model. Bone 2008, 42:921-931.
164. Virk M.S., Sugiyama O., Park S.H., Gambhir S.S., Adams D.J., Drissi H., et al. "Same day" ex-vivo regional gene therapy: a novel strategy to enhance bone repair. Mol Ther 2011, 19:960-968.
165. Wang K.-C., Wu J.-C., Chung Y.-C., Ho Y.-C., Chang M.D., Hu Y.-C. Baculovirus as a highly efficient gene delivery vector for the expression of hepatitis delta virus antigens in mammalian cells. Biotechnol Bioeng 2005, 89:464-473.
166. Wang Y.H., Ho M.L., Chang J.K., Chu H.C., Lai S.C., Wang G.J. Microporation is a valuable transfection method for gene expression in human adipose tissue-derived stem cells. Mol Ther 2009, 17:302-308.
167. Wang X., Oyane A., Tsurushima H., Sogo Y., Li X., Ito A. BMP-2 and ALP gene expression induced by a BMP-2 gene-fibronectin-apatite composite layer. Biomed Mater 2011, 6:045004.
168. Wright V.J., Peng H.R., Usas A., Young B., Gearhart B., Cummins J., et al. BMP4-expressing muscle-derived stem cells differentiate into osteogenic lineage and improve bone healing in immunocompetent mice. Mol Ther 2002, 6:169-178.
169. Xu X.L., Tang T., Dai K., Zhu Z., Guo X.E., Yu C., et al. Immune response and effect of adenovirus-mediated human BMP-2 gene transfer on the repair of segmental tibial bone defects in goats. Acta Orthop 2005, 76:637-646.
170. Yang F., Green J.J., Dinio T., Keung L., Cho S.W., Park H., et al. Gene delivery to human adult and embryonic cell-derived stem cells using biodegradable nanoparticulate polymeric vectors. Gene Ther 2009, 16:533-546.
171. Yazici C., Takahata M., Reynolds D.G., Xie C., Samulski R.J., Samulski J., et al. Self-complementary AAV2.5-BMP2-coated femoral allografts mediated superior bone healing versus live autografts in mice with equivalent biomechanics to unfractured femur. Mol Ther 2011, 19:1416-1425.
172. Ye J.-H., Xu Y.-J., Gao J., Yan S.-G., Zhao J., Tu Q., et al. Critical-size calvarial bone defects healing in a mouse model with silk scaffolds and SATB2-modified iPSCs. Biomaterials 2011, 32:5065-5076.
173. Yla-Herttuala S. Endgame: Glybera finally recommended for approval as the first gene therapy drug in the European Union. Mol Ther 2012, 20:1831-1832.
174. Young S., Patel Z.S., Kretlow J.D., Murphy M.B., Mountziaris P.M., Baggett L.S., et al. Dose effect of dual delivery of vascular endothelial growth factor and bone morphogenetic protein-2 on bone regeneration in a rat critical-size defect model. Tissue Eng Part A 2009, 15:2347-2362.
175. Zeng J.-M., Du J., Lin J.-K., Bak X.-Y., Wu C.-X., Wang S. High-efficiency transient transduction of human embryonic stem cell-derived neurons with baculoviral vectors. Mol Ther 2009, 17:1585-1593.
176. Zhang X.P., Xie C., Lin A.S.P., Ito H., Awad H., Lieberman J.R., et al. Periosteal progenitor cell fate in segmental cortical bone graft transplantations: implications for functional tissue engineering. J Bone Miner Res 2005, 20:2124-2137.
177. Zhang Y., Shi B., Li C., Wang Y., Chen Y., Zhang W., et al. The synergetic bone-forming effects of combinations of growth factors expressed by adenovirus vectors on chitosan/collagen scaffolds. J Control Release 2009, 136:172-178.
178. Zhang W., Tsurushima H., Oyane A., Yazaki Y., Sogo Y., Ito A., et al. BMP-2 gene-fibronectin-apatite composite layer enhances bone formation. J Biomed Sci 2011, 18:62.
179. Zhang Y., Fan W., Nothdurft L., Wu C., Zhou Y., Crawford R., et al. In vitro and in vivo evaluation of adenovirus combined silk fibroin scaffolds for bone morphogenetic protein-7 gene delivery. Tissue Eng Part C Methods 2011, 17:789-797.
180. Zhang Y., Cheng N., Miron R., Shi B., Cheng X. Delivery of PDGF-B and BMP-7 by mesoporous bioglass/silk fibrin scaffolds for the repair of osteoporotic defects. Biomaterials 2012, 33:6698-6708.
181. Zhao Z., Wang Z., Ge C., Krebsbach P., Franceschi R.T. Healing cranial defects with AdRunx2-transduced marrow stromal cells. J Dent Res 2007, 86:1207-1211.
182. Zou D., Zhang Z., He J., Zhu S., Wang S., Zhang W., et al. Repairing critical-sized calvarial defects with BMSCs modified by a constitutively active form of hypoxia-inducible factor-1alpha and a phosphate cement scaffold. Biomaterials 2011, 32:9707-9718.
183. Zou D., Zhang Z., Ye D., Tang A., Deng L., Han W., et al. Repair of critical-sized rat calvarial defects using genetically engineered BMSCs overexpressing HIF-1α. Stem Cells 2011, 29:1380-1390.
184. Zou D., Zhang Z., He J., Zhang K., Ye D., Han W., et al. Blood vessel formation in the tissue-engineered bone with the constitutively active form of HIF-1α mediated BMSCs. Biomaterials 2012, 33:2097-2108.