Genetic enhancement of fracture repair: Healing of an experimental segmental defect by adenoviral transfer of the BMP-2 gene

Gene Therapy

Volumn 7, Issue 9, 2000, Pages 734-739

  Baltzer, A W A ^a   Lattermann, C ^b   Whalen, J D ^b   Wooley, P ^c   Weiss, K ^b   Grimm, M ^c   Ghivizzani, S C ^b   Robbins, P D ^b   Evans, C H ^b  

^a HEINRICH HEINE UNIVERSITY   (Germany)

^b UNIVERSITY OF PITTSBURGH SCHOOL OF MEDICINE   (United States)

^c WAYNE STATE UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Bone healing; Bone morphogenetic protein; Direct gene delivery

Indexed keywords

BONE MORPHOGENETIC PROTEIN; COMPLEMENTARY DNA; LUCIFERASE; VIRUS VECTOR;

ADENOVIRUS; ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ARTICLE; BIOMECHANICS; BONE BOWING; BONE DEVELOPMENT; BONE STRENGTH; CONTROLLED STUDY; FEMUR; FRACTURE HEALING; FRACTURE TREATMENT; GENE TRANSFER; HISTOLOGY; NONHUMAN; OSSIFICATION; PRIORITY JOURNAL; RABBIT; RADIOGRAPHY; STATISTICAL ANALYSIS;

ADENOVIRIDAE; ANIMALS; BIOMECHANICS; BONE MORPHOGENETIC PROTEINS; FEMORAL FRACTURES; FEMUR; GENE THERAPY; RABBITS; TRANSFECTION; TRANSFORMING GROWTH FACTOR BETA; WOUND HEALING;

ADENOVIRIDAE; ANIMALIA; ORYCTOLAGUS CUNICULUS;

EID: 85047699915     PISSN: 09697128     EISSN: None     Source Type: Journal    
DOI: 10.1038/sj.gt.3301166     Document Type: Article

References (29)

1. Albertson KS, Medoff RJ, Mitsumaga MM. The use of periosteally vascularized autografts to augment the fixation of large segmental allografts. Clin Orthop Rel Res 1991; 269: 113-114.
2. Zaslav KR, Meinhard BP. Management of resistant pseudarthrosis of long bones. Clin Orthop Rel Res 1988; 233: 234-242.
3. Bostrom M et al. Use of BMP-2 in the rabbit ulnar nonunion model. Clin Orthop Rel Res 1996; 327: 272-282.
4. Moroni A, Rollo G, Guzzardella M, Zinghi G. Surgical treatment of isolated forearm non-union with segmental bone loss. Injury 1997; 28: 497-504.
5. Südkamp NP et al. Incidence of non-unions in open fractures: Analysis of 948 open fractures. Akt Traumatol 1993; 23: 59-67.
6. Gerhart TN et al. Healing segmental femoral defects in sheep using recombinant human one morphogenetic protein. Clin Orthop Rel Res 1993; 293: 317-326.
7. Einhorn TA. Enhancement of fracture healing. J Bone Joint Surg 1995; 77A: 940-955.
8. Ripamonti U et al. Recombinant transforming growth factor-β1 induces endochondral bone in the baboon and synergizes with recombinant osteogenic protein-1 (bone morphogenetic protein-7) to initiate rapid bone formation. J Bone Min Res 1997; 12: 1584-1595.
9. Yasko AW et al. The healing of segmental bone defects, induced by recombinant human bone morphogenetic protein (rhBMP). J Bone Joint Surg 1992; 74-A: 659-670.
10. Sampath TK et al. TGF-β superfamily proteins induce endochondral bone formation in mammals. Proc Natl Acad Sci USA 1993; 84: 7109-7113.
11. Hammonds RG et al. Bone inducing activity of mature BMP-2b produced from a hybrid BMP-2 a/b percusor. Mol Endocrinol 1991; 5: 149-155.
12. Evans CH, Robbins PD. Possible orthopaedic applications of gene therapy. J Bone Joint Surg 1995; 77-A: 1103-1114.
13. Niyibizi C et al. Potential role for gene therapy in the enhancement of fracture healing. Clin Orthop Rel Res 1998; 355S: 148-153.
14. Lieberman JR et al. Regional gene therapy with a BMP-2 producing murine stromal cell line induces heterotopic and orthotopic bone formation in rodents. J Orthop Res 1998; 16: 330-339.
15. Lieberman JR et al. The effect of regional gene therapy with bone morphogenetic protein-2 producing bone-marrow cells on the repair of segmental femoral defects in rats. J Bone Joint Surg 1999; 81A: 905-917.
16. Fang J et al. Stimulation of new bone formation by direct transfer of osteogenic plasmid genes. Proc Natl Acad Sci USA 1996; 93: 5753-5758.
17. Bonadio J, Smiley E, Patil P, Goldstein S. Localized direct plasmid gene delivery in vivo: Prolonged therapy results in reproducible tissue regeneration. Nature Med 1999; 5: 753-759.
18. Boden SD et al. Lumbar spine fusion by local gene therapy with a cDNA encoding a novel osteoinductive protein (LMP-1). Spine 1998; 23: 2486-2492.
19. Alden TD et al. In vivo endochondral bone formation using a bone morphogenetic protein 2 adenoviral vector. Hum Gene Ther 1999; 10: 2245-2253.
20. Musgrave DS et al. Adenovirus-mediated direct gene therapy with bone morphogenetic protein-2 produces bone. Bone 1999; 24: 541-547.
21. Baltzer AWA et al. A gene therapy approach to accelerating bone healing: Evaluation of gene expression in a New Zealand white rabbit model. Knee Surg Sports Traum Arthrosc 1999; 7: 197-200.
22. Mason JM et al. Expression of human bone morphogenetic protein 7 in primary rabbit periosteal cells: Potential utility in gene therapy for osteochondral repair. Gene Therapy 1998; 5: 275-282.
23. Wang EA. Bone morphogenetic proteins (BMPs): Therapeutic potential in healing bony defects. Trends Biotechnol 1993; 11: 379-383.
24. Aspenberg P, Wang E, Thorgren KG. Bone morphogenetic protein induces bone in the squirrel monkey, but bone matrix does not. Acta Orthop Scand 1992; 63: 619-622.
25. Lind M, Eriksen EF, Bünger C. Bone morphogenetic protein-2 but not bone morphogenetic protein-4 and -6 stimulates chemotactic migration of human osteoblasts, human marrow osteoblasts, and U2-OS cells. Bone 1996; 18: 53-57.
26. Yamaguchi A et al. Recombinant human BMP-2 stimulates osteoblastic maturation and inhibits myogenic differentiation in vitro. J Cell Biol 1991; 113: 681-687.
27. Katagiri T et al. BMP-2 converts the differentiation pathway of C2C12 myoblasts into the osteoblast lineage. J Cell Biol 1993; 6: 1755-1766.
28. Hardy S et al. Construction of adenovirus vectors through Cre-Lox recombination. J Virol 1997; 71: 1842-1849.
29. Balk ML et al. Effect of rhBMP-2 on the osteogenic potential of bone marrow stromal cells from an osteogenesis imperfecta mouse (oim). Bone 1997; 21: 7-15.