Osteogenic gene regulation and relative acceleration of healing by adenoviral-mediated transfer of human BMP-2 or -6 in equine osteotomy and ostectomy models

Journal of Orthopaedic Research

Volumn 26, Issue 6, 2008, Pages 764-771

  Ishihara, Akikazu ^a   Shields, Kathleen M ^b   Litsky, Alan S ^a   Mattoon, John S ^c   Weisbrode, Steven E ^a   Bartlett, Jeffrey S ^d   Bertone, Alicia L ^a  

^a OHIO STATE UNIVERSITY   (United States)

^b PFIZER INC   (United States)

^c WASHINGTON STATE UNIVERSITY   (United States)

^d THE RESEARCH INSTITUTE AT NATIONWIDE CHILDREN S HOSPITAL   (United States)

Author keywords

Adenovirus; BMP 2; BMP 6; Bone healing; Gene chip

Indexed keywords

BETA GALACTOSIDASE; BONE MORPHOGENETIC PROTEIN 2; BONE MORPHOGENETIC PROTEIN 6; CATHEPSIN H; GELSOLIN; TRANSFORMING GROWTH FACTOR BETA1; BONE MORPHOGENETIC PROTEIN; TRANSFORMING GROWTH FACTOR BETA;

ADENOVIRUS; ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; BONE DEVELOPMENT; CALLUS; COMPARATIVE STUDY; COMPUTER ASSISTED TOMOGRAPHY; CONTROLLED STUDY; FRACTURE HEALING; GENE CONTROL; GENE EXPRESSION; HORSE; METATARSAL BONE; NONHUMAN; OSTEOTOMY; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; RADIODIAGNOSIS; TORQUE; UPREGULATION; ANIMAL; BIOMECHANICS; DISEASE MODEL; DNA MICROARRAY; FRACTURE; GENE EXPRESSION REGULATION; GENE THERAPY; GENE TRANSFER; GENETICS; HUMAN; LACTOSE OPERON; MECHANICAL TORSION; METHODOLOGY; PATHOPHYSIOLOGY; PHYSIOLOGY; RADIOGRAPHY;

ADENOVIRIDAE; ANIMALS; BIOMECHANICS; BONE MORPHOGENETIC PROTEINS; BONY CALLUS; DISEASE MODELS, ANIMAL; FRACTURE HEALING; FRACTURES, BONE; GENE EXPRESSION REGULATION; GENE THERAPY; GENE TRANSFER TECHNIQUES; HORSES; HUMANS; LAC OPERON; METATARSAL BONES; OLIGONUCLEOTIDE ARRAY SEQUENCE ANALYSIS; OSTEOGENESIS; OSTEOTOMY; TOMOGRAPHY, X-RAY COMPUTED; TORQUE; TORSION, MECHANICAL; TRANSFORMING GROWTH FACTOR BETA;

EID: 46449095585     PISSN: 07360266     EISSN: None     Source Type: Journal    
DOI: 10.1002/jor.20585     Document Type: Article

References (26)

1. Villavicencio AT, Burneikiene S, Nelson EL, et al. 2005. Safety of transforaminal lumbar interbody fusion and intervertebral recombinant human bone morphogenetic protein-2. J Neurosurg Spine 3:436-443.
2. Jones AL, Bucholz RW, Bosse MJ, et al. 2006. Recombinant human BMP-2 and allograft compared with autogenous bone graft for reconstruction of diaphyseal tibial fractures with cortical defects. A randomized, controlled trial. J Bone Joint Surg Am 88:1431-1441.
3. Baltzer AW, Lattermann C, Whalen JD, et al. 2000. Genetic enhancement of fracture repair: healing of an experimental segmental defect by adenoviral transfer of the BMP-2 gene. Gene Ther 7:734-739.
4. Betz OB, Betz VM, Nazarian A, et al. 2006. Direct percutaneous gene delivery to enhance healing of segmental bone defects. J Bone Joint Surg Am 88:355-365.
5. Bertone AL, Pittman DD, Bouxsein ML, et al. 2004. Adenoviral-mediated transfer of human BMP-6 gene accelerates healing in a rabbit ulnar osteotomy model. J Orthop Res 22:1261-1270.
6. Dunn CA, Jin Q, Taba M Jr, et al. 2005. BMP gene delivery for alveolar bone engineering at dental implant defects. Mol Ther 11:294-299.
7. Varady P, Li JZ, Cunningham M, et al. 2001. Morphologic analysis of BMP-9 gene therapy-induced osteogenesis. Hum Gene Ther 12:697-710.
8. Zhang Y, An HS, Thonar EJ, et al. 2006. Comparative effects of bone morphogenetic proteins and sox9 overexpression on extracellular matrix metabolism of bovine nucleus pulposus cells. Spine 31:2173-2179.
9. Jane JA Jr, Dunford BA, Kron A, et al. 2002. Ectopic osteogenesis using adenoviral bone morphogenetic protein (BMP)-4 and BMP-6 gene transfer. Mol Ther 6:464-470.
10. Kang Q, Sun MH, Cheng H, et al. 2004. Characterization of the distinct orthotopic bone-forming activity of 14 BMPs using recombinant adenovirus-mediated gene delivery. Gene Ther 11:1312-1320.
11. Zachos TA, Shields KM, Bertone AL. Gene-mediated osteogenic differentiation of stem cells by bone morphogenetic proteins-2 or -6. J Orthop Res 24:1279-1291.
12. Li JZ, Li H, Sasaki T, et al. 2005. Osteogenic potential of five different recombinant human bone morphogenetic protein adenoviral vectors in the rat. Gene Ther 10:1735-1743.
13. Lacroix D, Prendergast PJ. 2002. A mechano-regulation model for tissue differentiation during fracture healing: analysis of gap size and loading. J Biomech 35:1163-1171.
14. Sasano Y, Mizoguchi I, Takahashi I, et al. 1997. BMPs induce endochondral ossification in rats when implanted ectopically within a carrier made of fibrous glass membrane. Anat Rec 247:472-478.
15. Egermann M, Lill CA, Griesbeck K, et al. 2006. Effect of BMP-2 gene transfer on bone healing in sheep. Gene Ther 13:1290-1299.
16. Heiner DE, Meyer MH, Frick SL, et al. 2006. Gene expression during fracture healing in rats comparing intramedullary fixation to plate fixation by DNA microarray. J Orthop Trauma 20:27-38.
17. Niikura T, Hak DJ, Reddi AH. 2006. Global gene profiling reveals a downregulation of BMP gene expression in experimental atrophic nonunions compared to standard healing fractures. J Orthop Res 24:1463-1471.
18. Clancy BM, Johnson JD, Lambert AJ, et al. 2003. A gene expression profile for endochondral bone formation: oligonucleotide microarrays establish novel connections between known genes and BMP-2-induced bone formation in mouse quadriceps. Bone 33:46-63.
19. Waselau M, Samii VF, Weisbrode SE, et al. 2007. Effects of a magnesium adhesive cement on bone stability and healing following a metatarsal osteotomy in horses. Am J Vet Res 68:370-378.
20. Les CM, Keyak JH, Stover SM, et al. 1994. Estimation of material properties in the equine metacarpus with use of quantitative computed tomography. J Orthop Res 12:822-833.
21. Gu W, Bertone AL. 2004. Generation and performance of an equine-specific large-scale gene expression microarray. Am J Vet Res 65:1664-1673.
22. Betz OB, Betz VM, Nazarian A, et al. 2007. Delayed administration of adenoviral BMP-2 vector improves the formation of bone in osseous defects. Gene Ther 14:1039-1044.
23. Ito T, Tokunaga K, Maruyama H, et al. 2003. Coxsackievirus and adenovirus receptor (CAR)-positive immature osteoblasts as targets of adenovirus-mediated gene transfer for fracture healing. Gene Ther 10:1623-1628.
24. Murnaghan M, McIlmurray L, Mushipe MT, et al. 2005. Time for treating bone fracture using rhBMP-2: a randomised placebo controlled mouse fracture trial. J Orthop Res. 23:625-631.
25. Cho TJ, Gerstenfeld LC, Einhorn TA. 2002. Differential temporal expression of members of the transforming growth factor beta superfamily during murine fracture healing. J Bone Miner Res 17:513-520.
26. Denker AE, Haas AR, Nicoll SB, et al. 1999. Chondrogenic differentiation of murine C3H10T1/2 multipotential mesenchymal cells: I. Stimulation by bone morphogenetic protein-2 in high-density micromass cultures. Differentiation 64:67-76.