Genetically modified mesenchymal stem cells induce mechanically stable posterior spine fusion

Tissue Engineering - Part A

Volumn 16, Issue 12, 2010, Pages 3679-3686

  Sheyn, Dima ^a   Rüthemann, Martin ^b   Mizrahi, Olga ^a   Kallai, Ilan ^a   Zilberman, Yoram ^a   Tawackoli, Wafa ^c   Kanim, Linda E A ^c   Zhao, Li ^c   Bae, Hyun ^c   Pelled, Gadi ^a,c   Snedeker, Jess G ^b,d   Gazit, Dan ^a,c  

^a HEBREW UNIVERSITY OF JERUSALEM   (Israel)

^b UNIVERSITY OF ZURICH   (Switzerland)

^c CEDARS SINAI MEDICAL CENTER   (United States)

^d ETH ZURICH   (Switzerland)

Author keywords

[No Author keywords available]

Indexed keywords

BIOCHEMICAL ENGINEERING; BIOMECHANICS; BONE; CELL CULTURE; COMPUTERIZED TOMOGRAPHY; FLOWCHARTING; HOT ROLLING; IMPLANTS (SURGICAL); MUSCLE; RIGIDITY; STEM CELLS; STIFFNESS;

BENDING STIFFNESS; BIOLOGICAL TREATMENT; BIOMECHANICAL TESTS; BONE FORMATION; BONE GRAFT; BONE MORPHOGENETIC PROTEIN-2; CONTROL GROUPS; FIXATION DEVICE; FOUR-POINT BENDING; FUSION GROUPS; GENETICALLY MODIFIED; HISTOLOGICAL ANALYSIS; LOCAL ANALYSIS; LUMBAR SPINES; MECHANICAL FIXATION; MECHANICALLY STABLE; MESENCHYMAL STEM CELL; MICROCOMPUTED TOMOGRAPHY; PARASPINAL MUSCLES; POSTERIOR SPINAL FUSIONS; SPINAL FUSION; SPINAL SEGMENTS; SPINE FUSION;

STAINLESS STEEL;

ANIMAL; ARTICLE; BIOMECHANICS; BONE DEVELOPMENT; CELL LINE; CYTOLOGY; FEMALE; GENETICS; IMMUNOHISTOCHEMISTRY; MESENCHYMAL STEM CELL; METABOLISM; MICRO-COMPUTED TOMOGRAPHY; MOUSE; PHYSIOLOGY; SPINE; SPINE FUSION;

ANIMALS; BIOMECHANICS; CELL LINE; FEMALE; IMMUNOHISTOCHEMISTRY; MESENCHYMAL STEM CELLS; MICE; OSTEOGENESIS; SPINAL FUSION; SPINE; X-RAY MICROTOMOGRAPHY;

EID: 78650038170     PISSN: 19373341     EISSN: 1937335X     Source Type: Journal    
DOI: 10.1089/ten.tea.2009.0786     Document Type: Article

References (38)

1. Sobajima, S., Kim, J.S., Gilbertson, L.G., and Kang, J.D. Gene therapy for degenerative disc disease. Gene Ther 11, 390, 2004.
2. Cassinelli, E.H., Hall, R.A., and Kang, J.D. Biochemistry of intervertebral disc degeneration and the potential for gene therapy applications. Spine J 1, 205, 2001.
3. Kraemer, J. Natural course and prognosis of intervertebral disc diseases. International Society for the Study of the Lumbar Spine Seattle, Washington, June 1994. Spine 20, 635, 1995.
4. Yoon, S.T., and Boden, S.D. Spine fusion by gene therapy. Gene Ther 11, 360, 2004.
5. Wang, M.C., Chan, L., Maiman, D.J., Kreuter, W., and Deyo, R.A. Complications and mortality associated with cervical spine surgery for degenerative disease in the United States. Spine 32, 342, 2007.
6. Kager, A.N., Marks, M., Bastrom, T., and Newton, P.O. Morbidity of iliac crest bone graft harvesting in adolescent deformity surgery. J Pediatr Orthop 26, 132, 2006.
7. Swan, M.C., and Goodacre, T.E. Morbidity at the iliac crest donor site following bone grafting of the cleft alveolus. Br J Oral Maxillofac Surg 44, 129, 2006.
8. Buttermann, G.R. Prospective nonrandomized comparison of an allograft with bone morphogenic protein versus an iliac-crest autograft in anterior cervical discectomy and fusion. Spine J 8, 426, 2008.
9. Betz, R.R., Petrizzo, A.M., Kerner, P.J., Falatyn, S.P., Clements, D.H., and Huss, G.K. Allograft versus no graft with a posterior multisegmented hook system for the treatment of idiopathic scoliosis. Spine 31, 121, 2006.
10. Fang, A., Hu, S.S., Endres, N., and Bradford, D.S. Risk factors for infection after spinal surgery. Spine 30, 1460, 2005.
11. Chen, N.F., Smith, Z.A., Stiner, E., Armin, S., Sheikh, H., and Khoo, L.T. Symptomatic ectopic bone formation after off-label use of recombinant human bone morphogenetic protein-2 in transforaminal lumbar interbody fusion. J Neuro-surg Spine 12, 40, 2010.
12. Deutsch, H. High-dose bone morphogenetic protein-induced ectopic abdomen bone growth. Spine J 10, e1, 2010.
13. Kim, D.H., and Vaccaro, A.R. Osteoporotic compression fractures of the spine; current options and considerations for treatment. Spine J 6, 479, 2006.
14. Gibson, J.N., Grant, I.C., and Waddell, G. The Cochrane review of surgery for lumbar disc prolapse and degenerative lumbar spondylosis. Spine 24, 1820, 1999.
15. Kim, H.S., Viggeswarapu, M., Boden, S.D., Liu, Y., Hair, G.A., Louis-Ugbo, J., Murakami, H., Minamide, A., Suh, D.Y., and Titus, L. Overcoming the immune response to permit ex vivo gene therapy for spine fusion with human type 5 adenoviral delivery of the LIM mineralization pro-tein-1 cDNA. Spine 28, 219, 2003.
16. Risbud, M.V., Shapiro, I.M., Guttapalli, A., Di Martino, A., Danielson, K.G., Beiner, J.M., Hillibrand, A., Albert, T.J., Anderson, D.G., and Vaccaro, A.R. Osteogenic potential of adult human stem cells of the lumbar vertebral body and the iliac crest. Spine 31, 83, 2006.
17. Nakajima, T., Iizuka, H., Tsutsumi, S., Kayakabe, M., and Takagishi, K. Evaluation of posterolateral spinal fusion using mesenchymal stem cells: differences with or without osteogenic differentiation. Spine 32, 2432, 2007.
18. Helm, G.A., and Gazit, Z. Future uses of mesenchymal stem cells in spine surgery. Neurosurg Focus 19, E13, 2005.
19. Gan, Y., Dai, K., Zhang, P., Tang, T., Zhu, Z., and Lu, J. The clinical use of enriched bone marrow stem cells combined with porous beta-tricalcium phosphate in posterior spinal fusion. Biomaterials 29, 3973, 2008.
20. Hasharoni, A., Zilberman, Y., Turgeman, G., Helm, G.A., Liebergall, M., and Gazit, D. Murine spinal fusion induced by engineered mesenchymal stem cells that conditionally express bone morphogenetic protein-2. J Neurosurg Spine 3, 47, 2005.
21. Sheyn, D., Kimelman-Bleich, N., Pelled, G., Zilberman, Y., Gazit, D., and Gazit, Z. Ultrasound-based nonviral gene delivery induces bone formation in vivo. Gene Ther 15, 257, 2008.
22. Dumont, R.J., Dayoub, H., Li, J.Z., Dumont, A.S., Kallmes, D.F., Hankins, G.R., and Helm, G.A. Ex vivo bone morpho-genetic protein-9 gene therapy using human mesenchymal stem cells induces spinal fusion in rodents. Neurosurgery 51, 1239, 2002.
23. Laurent, J.J., Webb, K.M., Beres, E.J., McGee, K., Li, J., van Rietbergen, B., and Helm, G.A. The use of bone morphoge-netic protein-6 gene therapy for percutaneous spinal fusion in rabbits. J Neurosurg Spine 1, 90, 2004.
24. Aslan, H., Zilberman, Y., Arbeli, V., Sheyn, D., Matan, Y., Liebergall, M., Zhong, J., Helm, G.A., Gazit, D., and Gazit, Z. Nucleofection-based ex vivo nonviral gene delivery to human stem cells as a platform for tissue regeneration. Tissue Eng 12, 877 2006.
25. Zuk, P.A., Zhu, M., Ashjian, P., De Ugarte, D.A., Huang, J.I., Mizuno, H., Alfonso, Z.C., Fraser, J.K., Benhaim, P., and Hedrick, M.H. Human adipose tissue is a source of multi-potent stem cells. Mol Biol Cell 13, 4279, 2002.
26. Pelled, G., Tai, K., Sheyn, D., Zilberman, Y., Kumbar, S., Nair, L.S., Laurencin, C.T., Gazit, D., and Ortiz, C. Structural and nanoindentation studies of stem cell-based tissue-engineered bone. J Biomech 40, 399, 2007.
27. Tai, K., Pelled, G., Sheyn, D., Bershteyn, A., Han, L., Kallai, I., Zilberman, Y., Ortiz, C., and Gazit, D. Nanobiomechanics of repair bone regenerated by genetically modified mesen-chymal stem cells. Tissue Eng Part A 14, 1709, 2008.
28. Moutsatsos, I.K., Turgeman, G., Zhou, S., Kurkalli, B.G., Pelled, G., Tzur, L., Kelley, P., Stumm, N., Mi, S., Muller, R., Zilberman, Y., and Gazit, D. Exogenously regulated stem cell-mediated gene therapy for bone regeneration. Mol Ther 3, 449, 2001.
29. Hildebrand, T., Laib, A., Muller, R., Dequeker, J., and Ruegsegger, P. Direct three-dimensional morphometric analysis of human cancellous bone: microstructural data from spine, femur, iliac crest, and calcaneus. J Bone Miner Res 14, 1167, 1999.
30. Muller, R., Hildebrand, T., and Ruegsegger, P. Non-invasive bone biopsy: a new method to analyse and display the three-dimensional structure of trabecular bone. Phys Med Biol 39, 145, 1994.
31. ASTM. Standard Test Method for Flexural Properties of Unreinforced and Reinforced Plastics and Electrical Insulating Materials by Four-Point Bending. D6272. ASTM, 2002, p. 519.
32. Cottrell, J.M., Van Der Meulen, M.C., Lane, J.M., and Myers, E.R. Assessing the stiffness of spinal fusion in animal models. HSS J 2, 12, 2006.
33. Beer, F.P., Johnston, E.R., and DeWolf, J.T. Mechanics of Materials. Burr Ridge, IL: McGraw-Hill Higher Education, 2006.
34. Turgeman, G., Pittman, D.D., Muller, R., Kurkalli, B.G., Zhou, S., Pelled, G., Peyser, A., Zilberman, Y., Moutsatsos, I.K., and Gazit, D. Engineered human mesenchymal stem cells: a novel platform for skeletal cell mediated gene therapy. J Gene Med 3, 240, 2001.
35. Fu, T.S., Chen, W.J., Chen, L.H., Lin, S.S., Liu, S.J., and Ueng, S.W. Enhancement of posterolateral lumbar spine fusion using low-dose rhBMP-2 and cultured marrow stromal cells. J Orthop Res 27, 380, 2009.
36. Gazit, D., Turgeman, G., Kelley, P., Wang, E., Jalenak, M., Zilberman, Y., and Moutsatsos, I. Engineered pluripotent mesenchymal cells integrate and differentiate in regenerating bone: a novel cell-mediated gene therapy. J Gene Med 1, 121, 1999.
37. Aslan, H., Zilberman, Y., Kandel, L., Liebergall, M., Os-kouian, R.J., Gazit, D., and Gazit, Z. Osteogenic differentiation of noncultured immunoisolated bone marrow-derived CD105\+ cells. Stem Cells 24, 1728, 2006.
38. Sheyn, D., Pelled, G., Zilberman, Y., Talasazan, F., Frank, J.M., Gazit, D., and Gazit, Z. Nonvirally engineered porcine adipose tissue-derived stem cells: use in posterior spinal fusion. Stem Cells 26, 1056, 2008.