MicroRNAs in vertebrate physiology and human disease

Annual Review of Genomics and Human Genetics

Volumn 8, Issue , 2007, Pages 215-239

  Chang, Tsung Cheng ^a   Mendell, Joshua T ^a  

^a JOHNS HOPKINS UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Cancer; Noncoding RNAs; Post transcriptional regulation; RNA interference

Indexed keywords

MESSENGER RNA; MICRORNA; UNTRANSLATED RNA; LET 7 MICRORNA, HUMAN; LET-7 MICRORNA, HUMAN; R 15 MICRORNA, HUMAN; R 155 MICRORNA, HUMAN; R 16 MICRORNA, HUMAN; R 17 MICRORNA, HUMAN; R 21 MICRORNA, HUMAN; R-15 MICRORNA, HUMAN; R-155 MICRORNA, HUMAN; R-16 MICRORNA, HUMAN; R-17 MICRORNA, HUMAN; R-21 MICRORNA, HUMAN; UNCLASSIFIED DRUG;

CANCER GENETICS; CANCER INHIBITION; FRAGILE X SYNDROME; GENE CONTROL; GENETIC VARIABILITY; HUMAN; HUMAN GENOME; NONHUMAN; REVIEW; RNA INTERFERENCE; RNA PROCESSING; RNA STABILITY; TRANSLATION REGULATION; VERTEBRATE; ANIMAL; BIOLOGICAL MODEL; BIOSYNTHESIS; GENETIC DISORDER; GENETICS; METABOLISM; MULTIGENE FAMILY; NEOPLASM; ONCOGENE; PHYSIOLOGY; SYNAPSE; TUMOR SUPPRESSOR GENE;

VERTEBRATA;

ANIMALS; FRAGILE X SYNDROME; GENES, TUMOR SUPPRESSOR; GENETIC DISEASES, INBORN; HUMANS; MICRORNAS; MODELS, BIOLOGICAL; MULTIGENE FAMILY; NEOPLASMS; ONCOGENES; SYNAPSES; VARIATION (GENETICS); VERTEBRATES;

EID: 35348821616     PISSN: 15278204     EISSN: None     Source Type: Book Series    
DOI: 10.1146/annurev.genom.8.080706.092351     Document Type: Review

References (149)

1. 2003. The International HapMap Project. Nature 426:789-96
2. Abbott AL, Alvarez-Saavedra E, Miska EA, Lau NC, Bartel DP, et al. 2005. The let-7 microRNA family members mir-48, mir-84, and mir-241 function together to regulate developmental timing in Caenorhabditis elegans. Dev. Cell 9:403-14
3. Abelson JF, Kwan KY, O'Roak BJ, Baek DY, Stillman AA, et al. 2005. Sequence variants in SLITRK1 are associated with Tourette's syndrome. Science 310:317-20
4. Ambros V. 2004. The functions of animal microRNAs. Nature 431:350-55
5. Andl T, Murchison EP, Liu F, Zhang Y, Yunta-Gonzalez M, et al. 2006. The miRNA-processing enzyme dicer is essential for the morphogenesis and maintenance of hair follicles. Curr. Biol. 16:1041-49
6. Aravin AA, Lagos-Quintana M, Yalcin A, Zavolan M, Marks D, et al. 2003. The small RNA profile during Drosophila melanogaster development. Dev. Cell 5:337-50
7. Ashraf SI, McLoon AL, Sclarsic SM, Kunes S. 2006. Synaptic protein synthesis associated with memory is regulated by the RISC pathway in Drosophila. Cell 124:191-205
8. Bagga S, Bracht J, Hunter S, Massirer K, Holtz J, et al. 2005. Regulation by let-7 and lin-4 miRNAs results in target mRNA degradation. Cell 122:553-63
9. Bartel DP. 2004. MicroRNAs: genomics, biogenesis, mechanism, and function. Cell 116:281-97
10. Baskerville S, Bartel DP. 2005. Microarray profiling of microRNAs reveals frequent coexpression with neighboring miRNAs and host genes. RNA 11:241-47
11. Bentwich I, Avniel A, Karov Y, Aharonov R, Gilad S, et al. 2005. Identification of hundreds of conserved and nonconserved human microRNAs. Nat. Genet. 37:766-70
12. Berezikov E, Guryev V, van de Belt J, Wienholds E, Plasterk RH, Cuppen E. 2005. Phylogenetic shadowing and computational identification of human microRNA genes. Cell 120:21-24
13. Bernstein E, Kim SY, Carmell MA, Murchison EP, Alcorn H, et al. 2003. Dicer is essential for mouse development. Nat. Genet. 35:215-17
14. Bohnsack MT, Czaplinski K, Gorlich D. 2004. Exportin 5 is a RanGTP-dependent dsRNA-binding protein that mediates nuclear export of premiRNAs. RNA 10:185-91
15. Brennecke J, Hipfner DR, Stark A, Russell RB, Cohen SM. 2003. bantam encodes a developmentally regulated microRNA that controls cell proliferation and regulates the proapoptotic gene hid in Drosophila. Cell 113:25-36
16. Brennecke J, Stark A, Russell RB, Cohen SM. 2005. Principles of microRNA-target recognition. PLoS Biol. 3:e85
17. Cai X, Hagedorn CH, Cullen BR. 2004. Human microRNAs are processed from capped, polyadenylated transcripts that can also function as mRNAs. RNA 10:1957-66
18. Calin GA, Dumitru CD, Shimizu M, Bichi R, Zupo S, et al. 2002. Frequent deletions and down-regulation of micro-RNA genes miR15 and miR16 at 13q14 in chronic lymphocytic leukemia. Proc. Natl. Acad. Sci. USA 99:15524-29
19. Calin GA, Ferracin M, Cimmino A, Di Leva G, Shimizu M, et al. 2005. A microRNA signature associated with prognosis and progression in chronic lymphocytic leukemia. N. Engl. J. Med. 353:1793-801
20. Calin GA, Sevignani C, Dumitru CD, Hyslop T, Noch E, et al. 2004. Human microRNA genes are frequently located at fragile sites and genomic regions involved in cancers. Proc. Natl. Acad. Sci. USA 101:2999-3004
21. Cao X, Yeo G, Muotri AR, Kuwabara T, Gage FH. 2006. Noncoding RNAs in the mammalian central nervous system. Annu. Rev. Neurosci. 29:77-103
22. Carmell MA, Xuan Z, Zhang MQ, Hannon GJ. 2002. The Argonaute family: tentacles that reach into RNAi, developmental control, stem cell maintenance, and tumorigenesis. Genes Dev. 16:2733-42
23. Caudy AA, Myers M, Hannon GJ, Hammond SM. 2002. Fragile X-related protein and VIG associate with the RNA interference machinery. Genes Dev. 16:2491-96
24. Chan JA, Krichevsky AM, Kosik KS. 2005. MicroRNA-21 is an antiapoptotic factor in human glioblastoma cells. Cancer Res. 65:6029-33
25. Chen CZ, Li L, Lodish HF, Bartel DP. 2004. MicroRNAs modulate hematopoietic lineage differentiation. Science 303:83-86
26. Chen JF, Mandel EM, Thomson JM, Wu Q, Callis TE, et al. 2006. The role of microRNA-1 and microRNA-133 in skeletal muscle proliferation and differentiation. Nat. Genet. 38:228-33
27. Chen K, Rajewsky N. 2006. Natural selection on human microRNA binding sites inferred from SNP data. Nat. Genet. 38:1452-6
28. Cimmino A, Calin GA, Fabbri M, Iorio MV, Ferracin M, et al. 2 005. miR-15 and miR-16 induce apoptosis by targeting BCL2. Proc. Natl. Acad. Sci. USA 102:13944-49
29. Clop A, Marcq F, Takeda H, Pirottin D, Tordoir X, et al. 2006. A mutation creating a potential illegitimate microRNA target site in the myostatin gene affects muscularity in sheep. Nat. Genet. 38:813-18
30. Cobb BS, Nesterova TB, Thompson E, Hertweck A, O'Connor E, et al. 2005. T cell lineage choice and differentiation in the absence of the RNase III enzyme Dicer. J. Exp. Med. 201:1367-73
31. Conaco C, Otto S, Han JJ, Mandel G. 2006. Reciprocal actions of REST and a microRNA promote neuronal identity. Proc. Natl. Acad. Sci. USA 103:2422-27
32. Costinean S, Zanesi N, Pekarsky Y, Tili E, Volinia S, et al. 2006. Pre-B cell proliferation and lymphoblastic leukemia/high-grade lymphoma in E(mu)-miR155 transgenic mice. Proc. Natl. Acad. Sci. USA 103:7024-29
33. Cummins JM, He Y, Leary RJ, Pagliarini R, Diaz LAJ, et al. 2006. The colorectal microRNAome. Proc. Natl. Acad. Sci. USA 103:3687-92
34. Dang CV. 1999. c-Myc target genes involved in cell growth, apoptosis, and metabolism. Mol. Cell. Biol. 19:1-11
35. Darnell JC, Fraser CE, Mostovetsky O, Stefani G, Jones TA, et al. 2005. Kissing complex RNAs mediate interaction between the Fragile-X mental retardation protein KH2 domain and brain polyribosomes. Genes Dev. 19:903-18
36. Darnell JC, Jensen KB, Jin P, Brown V, Warren ST, Darnell RB. 2001. Fragile X mental retardation protein targets G quartet mRNAs important for neuronal function. Cell 107:489-99
37. Denli AM, Tops BB, Plasterk RH, Ketting RF, Hannon GJ. 2004. Processing of primary microRNAs by the microprocessor complex. Nature 432:231-35
38. Dews M, Homayouni A, Yu D, Murphy D, Sevignani C, et al. 2006. Augmentation of tumor angiogenesis by a Myc-activated microRNA cluster. Nat. Genet. 38:1060-65
39. Doench JG, Petersen CP, Sharp PA. 2003. siRNAs can function as miRNAs. Genes Dev. 17:438-42
40. Dohner H, Stilgenbauer S, Benner A, Leupolt E, Krober A, et al. 2000. Genomic aberrations and survival in chronic lymphocytic leukemia. N. Engl. J. Med. 343:1910-16
41. Dong JT, Boyd JC, Frierson HFJ. 2001. Loss of heterozygosity at 13q14 and 13q21 in high grade, high stage prostate cancer. Prostate 49:166-71
42. Eis PS, Tam W, Sun L, Chadburn A, Li Z, et al. 2005. Accumulation of miR-155 and BIC RNA in human B cell lymphomas. Proc. Natl. Acad. Sci. USA 102:3627-32
43. Elbashir SM, Harborth J, Lendeckel W, Yalcin A, Weber K, Tuschl T. 2001. Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells. Nature 411:494-98
44. Elbashir SM, Lendeckel W, Tuschl T. 2001. RNA interference is mediated by 21- and 22-nucleotide RNAs. Genes Dev. 15:188-200
45. Esau C, Davis S, Murray SF, Yu XX, Pandey SK, et al. 2006. miR-122 regulation of lipid metabolism revealed by in vivo antisense targeting. Cell Metab. 3:87-98
46. Esquela-Kerscher A, Slack FJ. 2006. Oncomirs - microRNAs with a role in cancer. Nat. Rev. Cancer 6:259-69
47. Farh KK, Grimson A, Jan C, Lewis BP, Johnston WK, et al. 2005. The widespread impact of mammalian microRNAs on mRNA repression and evolution. Science 310:1817-21
48. Ferguson EL, Sternberg PW, Horvitz HR. 1987. A genetic pathway for the specification of the vulval cell lineages of Caenorhabditis elegans. Nature 326:259-67
49. Fire A, Xu S, Montgomery MK, Kostas SA, Driver SE, Mello CC. 1998. Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans. Nature 391:806-11
50. Garber K, Smith KT, Reines D, Warren ST. 2006. Transcription, translation and fragile X syndrome. Curr. Opin. Genet. Dev. 16:270-75
51. Giraldez AJ, Cinalli RM, Glasner ME, Enright AJ, Thomson JM, et al. 2005. MicroRNAs regulate brain morphogenesis in zebrafish. Science 308:833-38
52. Gregory RI, Yan KP, Amuthan G, Chendrimada T, Doratotaj B, et al. 2004. The microprocessor complex mediates the genesis of microRNAs. Nature 432:235-40
53. Griffiths-Jones S. 2006. miRBase: the microRNA sequence database. Methods Mol. Biol. 342:129-38
54. Grishok A, Pasquinelli AE, Conte D, Li N, Parrish S, et al. 2001. Genes and mechanisms related to RNA interference regulate expression of the small temporal RNAs that control C. elegans developmental timing. Cell 106:23-34
55. Harfe BD, McManus MT, Mansfield JH, Hornstein E, Tabin CJ. 2005. The RNaseIII enzyme Dicer is required for morphogenesis but not patterning of the vertebrate limb. Proc. Natl. Acad. Sci. USA 102:10898-903
56. Harris KS, Zhang Z, McManus MT, Harfe BD, Sun X. 2006. Dicer function is essential for lung epithelium morphogenesis. Proc. Natl. Acad. Sci. USA 103:2208-13
57. Hayashita Y, Osada H, Tatematsu Y, Yamada H, Yanagisawa K, et al. 2005. Apolycistronic microRNA cluster, miR-17-92, is overexpressed in human lung cancers and enhances cell proliferation. Cancer Res. 65:9628-32
58. He H, Jazdzewski K, Li W, Liyanarachchi S, Nagy R, et al. 2005. The role of microRNA genes in papillary thyroid carcinoma. Proc. Natl. Acad. Sci. USA 102:19075-80
59. He L, Thomson JM, Hemann MT, Hernando-Monge E, Mu D, et al. 2005. A microRNA polycistron as a potential human oncogene. Nature 435:828-33
60. Horvitz HR, Sulston JE. 1980. Isolation and genetic characterization of cell-lineage mutants of the nematode Caenorhabditis elegans. Genetics 96:435-54
61. Houbaviy HB, Murray MF, Sharp PA. 2003. Embryonic stem cell-specific microRNAs. Dev. Cell 5:351-58
62. Hutvagner G, McLachlan J, Pasquinelli AE, Balint E, Tuschl T, Zamore PD. 2001. A cellular function for the RNA-interference enzyme Dicer in the maturation of the let-7 small temporal RNA. Science 293:834-38
63. Hutvagner G, Simard MJ, Mello CC, Zamore PD. 2004. Sequence-specific inhibition of small RNA function. PLoS Biol. 2:E98
64. Hutvagner G, Zamore PD. 2002. A microRNA in a multiple-turnover RNAi enzyme complex. Science 297:2056-60
65. Ishizuka A, Siomi MC, Siomi H. 2002. A Drosophila fragile X protein interacts with components of RNAi and ribosomal proteins. Genes Dev. 16:2497-508
66. Jin P, Alisch RS, Warren ST. 2004. RNA and microRNAs in fragile X mental retardation. Nat. Cell Biol. 6:1048-53
67. Jin P, Zarnescu DC, Ceman S, Nakamoto M, Mowrey J, et al. 2004. Biochemical and genetic interaction between the fragile X mental retardation protein and the microRNA pathway. Nat. Neurosci. 7:113-17
68. John B, Enright AJ, Aravin A, Tuschl T, Sander C, Marks DS. 2004. Human microRNA targets. PLoS Biol. 2:e363
69. Johnson SM, Grosshans H, Shingara J, Byrom M, Jarvis R, et al. 2005. RAS is regulated by the let-7 microRNA family. Cell 120:635-47
70. Jones-Rhoades MW, Bartel DP. 2004. Computational identification of plant microRNAs and their targets, including a stress-induced miRNA. Mol. Cell 14:787-99
71. Kanellopoulou C, Muljo SA, Kung AL, Ganesan S, Drapkin R, et al. 2005. Dicer-deficient mouse embryonic stem cells are defective in differentiation and centromeric silencing. Genes Dev. 19:489-501
72. Kent OA, Mendell JT. 2006. A small piece in the cancer puzzle: microRNAs as tumor suppressors and oncogenes. Oncogene 25:6188-96
73. Ketting RF, Fischer SE, Bernstein E, Sijen T, Hannon GJ, Plasterk RH. 2001. Dicer functions in RNA interference and in synthesis of small RNA involved in developmental timing in C. elegans. Genes Dev. 15:2654-59
74. Khvorova A, Reynolds A, Jayasena SD. 2003. Functional siRNAs and miRNAs exhibit strand bias. Cell 115:209-16
75. Kim J, Krichevsky A, Grad Y, Hayes GD, Kosik KS, et al. 2004. Identification of many microRNAs that copurify with polyribosomes in mammalian neurons. Proc. Natl. Acad. Sci. USA 101:360-65
76. Kim VN. 2005. MicroRNA biogenesis: coordinated cropping and dicing. Nat. Rev. Mol. Cell. Biol. 6:376-85
77. Kiriakidou M, Nelson PT, Kouranov A, Fitziev P, Bouyioukos C, et al. 2004. A combined computational-experimental approach predicts human microRNA targets. Genes Dev. 18:1165-78
78. Kloosterman WP, Plasterk RH. 2006. The diverse functions of microRNAs in animal development and disease. Dev. Cell 11:441-50
79. Krutzfeldt J, Rajewsky N, Braich R, Rajeev KG, Tuschl T, et al. 2005. Silencing of microRNAs in vivo with 'antagomirs'. Nature 438:685-89
80. Kwon C, Han Z, Olson EN, Srivastava D. 2005. MicroRNA1 influences cardiac differentiation in Drosophila and regulates Notch signaling. Proc. Natl. Acad. Sci. USA 102:18986-91
81. Lagos-Quintana M, Rauhut R, Lendeckel W, Tuschl T. 2001. Identification of novel genes coding for small expressed RNAs. Science 294:853-58
82. Lagos-Quintana M, Rauhut R, Yalcin A, Meyer J, Lendeckel W, Tuschl T. 2002. Identification of tissue-specific microRNAs from mouse. Curr. Biol. 12:735-39
83. Lai EC. 2004. Predicting and validating microRNA targets. Genome Biol. 5:115
84. Lai EC, Tomancak P, Williams RW, Rubin GM. 2003. Computational identification of Drosophila microRNA genes. Genome Biol. 4:R42
85. Landthaler M, Yalcin A, Tuschl T. 2004. The human DiGeorge syndrome critical region gene 8 and its D. melanogaster homolog are required for miRNA biogenesis. Curr. Biol. 14:2162-67
86. Lau NC, Lim LP, Weinstein EG, Bartel DP. 2001. An abundant class of tiny RNAs with probable regulatory roles in Caenorhabditis elegans. Science 294:858-62
87. Leaman D, Chen PY, Fak J, Yalcin A, Pearce M, et al. 2005. Antisense-mediated depletion reveals essential and specific functions of microRNAs in Drosophila development. Cell 121:1097-08
88. Lee RC, Ambros V. 2001. An extensive class of small RNAs in Caenorhabditis elegans. Science 294:862-64
89. Lee RC, Feinbaum RL, Ambros V. 1993. The C. elegans heterochronic gene lin-4 encodes small RNAs with antisense complementarity to lin-14. Cell 75:843-54
90. Lee Y, Ahn C, Han J, Choi H, Kim J, et al. 2003. The nuclear RNase III Drosha initiates microRNA processing. Nature 425:415-19
91. Lee Y, Kim M, Han J, Yeom KH, Lee S, et al. 2004. MicroRNA genes are transcribed by RNA polymerase II. EMBO J. 23:4051-60
92. Lewis BP, Burge CB, Bartel DP. 2005. Conserved seed pairing, often flanked by adenosines, indicates that thousands of human genes are microRNA targets. Cell 120:15-20
93. Lewis BP, Shih IH, Jones-Rhoades MW, Bartel DP, Burge CB. 2003. Prediction of mammalian microRNA targets. Cell 115:787-98
94. Lim LP, Lau NC, Garrett-Engele P, Grimson A, Schelter JM, et al. 2005. Microarray analysis shows that some microRNAs downregulate large numbers of target mRNAs. Nature 433:769-73
95. Lim LP, Lau NC, Weinstein EG, Abdelhakim A, Yekta S, et al. 2003. The microRNAs of Caenorhabditis elegans. Genes Dev. 17:991-1008
96. Liu J, Carmell MA, Rivas FV, Marsden CG, Thomson JM, et al. 2004. Argonaute2 is the catalytic engine of mammalian RNAi. Science 305:1437-41
97. Llave C, Xie Z, Kasschau KD, Carrington JC. 2002. Cleavage of Scarecrow-like mRNA targets directed by a class of Arabidopsis miRNA. Science 297:2053-56
98. Lu J, Getz G, Miska EA, Alvarez-Saavedra E, Lamb J, et al. 2005. MicroRNA expression profiles classify human cancers. Nature 435:834-38
99. Lund E, Guttinger S, Calado A, Dahlberg JE, Kutay U. 2004. Nuclear export of microRNA precursors. Science 303:95-98
100. McPherron AC, Lawler AM, Lee SJ. 1997. Regulation of skeletal muscle mass in mice by a new TGF-beta superfamily member. Nature 387:83-90
101. Meister G, Landthaler M, Dorsett Y, Tuschl T. 2004. Sequence-specific inhibition of microRNA- and siRNA-induced RNA silencing. RNA 10:544-50
102. Meister G, Landthaler M, Patkaniowska A, Dorsett Y, Teng G, Tuschl T. 2004. Human Argonaute2 mediates RNA cleavage targeted by miRNAs and siRNAs. Mol. Cell 15:185-97
103. Mourelatos Z, Dostie J, Paushkin S, Sharma A, Charroux B, et al. 2002. miRNPs: a novel class of ribonucleoproteins containing numerous microRNAs. Genes Dev. 16:720-28
104. Muljo SA, Ansel KM, Kanellopoulou C, Livingston DM, Rao A, Rajewsky K. 2005. Aberrant T cell differentiation in the absence of Dicer. J. Exp. Med. 202:261-69
105. Murchison EP, Partridge JF, Tarn OH, Cheloufi S, Hannon GJ. 2005. Characterization of Dicer-deficient murine embryonic stem cells. Proc. Natl. Acad. Sci. USA 102:12135-40
106. Naguibneva I, Ameyar-Zazoua M, Polesskaya A, Ait-Si-Ali S, Groisman R, et al. 2006. The microRNA miR-181 targets the homeobox protein Hox-A11 during mammalian myoblast differentiation. Nat. Cell Biol. 8:278-84
107. Nakajima N, Takahashi T, Kitamura R, Isodono K, Asada S, et al. 2006. MicroRNA-1 facilitates skeletal myogenic differentiation without affecting osteoblastic and adipogenic differentiation. Biochem. Biophys. Res. Commun. 350:1006-12
108. O'Donnell KA, Wentzel EA, Zeller KI, Dang CV, Mendell JT. 2005. c-Myc-regulated microRNAs modulate E2F1 expression. Nature 435:839-43
109. Olsen PH, Ambros V. 1999. The lin-4 regulatory RNA controls developmental timing in Caenorhabditis elegans by blocking LIN-14 protein synthesis after the initiation of translation. Dev. Biol. 216:671-80
110. Ota A, Tagawa H, Karnan S, Tsuzuki S, Karpas A, et al. 2004. Identification and characterization of a novel gene, C13orf25, as a target for 13q31-q32 amplification in malignant lymphoma. Cancer Res. 64:3087-95
111. Parker JS, Roe SM, Barford D. 2004. Crystal structure of a PIWI protein suggests mechanisms for siRNA recognition and slicer activity. EMBO J. 23:4727-37
112. Pasquinelli AE, Reinhart BJ, Slack F, Martindale MQ, Kuroda MI, et al. 2000. Conservation of the sequence and temporal expression of let-7 heterochronic regulatory RNA. Nature 408:86-89
113. Petersen CP, Bordeleau ME, Pelletier J, Sharp PA. 2006. Short RNAs repress translation after initiation in mammalian cells. Mol. Cell 21:533-42
114. Pillai RS, Artus CG, Filipowicz W. 2004. Tethering of human Ago proteins to mRNA mimics the miRNA-mediated repression of protein synthesis. RNA 10:1518-25
115. Pillai RS, Bhattacharyya SN, Artus CG, Zoller T, Cougot N, et al. 2005. Inhibition of translational initiation by Let-7 MicroRNA in human cells. Science 309:1573-76
116. Poy MN, Eliasson L, Krutzfeldt J, Kuwajima S, Ma X, et al. 2004. A pancreatic islet-specific microRNA regulates insulin secretion. Nature 432:226-30
117. Rao PK, Kumar RM, Farkhondeh M, Baskerville S, Lodish HF. 2006. Myogenic factors that regulate expression of muscle-specific microRNAs. Proc. Natl. Acad. Sci. USA 103:8721-26
118. Reinhart BJ, Slack FJ, Basson M, Pasquinelli AE, Bettinger JC, et al. 2000. The 21-nucleotide let-7 RNA regulates developmental timing in Caenorhabditis elegans. Nature 403:901-6
119. Rhoades MW, Reinhart BJ, Lim LP, Burge CB, Bartel B, Bartel DP. 2002. Prediction of plant microRNA targets. Cell 110:513-20
120. Rodriguez A, Griffiths-Jones S, Ashurst JL, Bradley A. 2004. Identification of mammalian microRNA host genes and transcription units. Genome Res. 14:1902-10
121. Schratt GM, Tuebing F, Nigh EA, Kane CG, Sabatini ME, et al. 2006. A brain-specific microRNA regulates dendritic spine development. Nature 439:283-89
122. Schwarz DS, Hutvagner G, Du T, Xu Z, Aronin N, Zamore PD. 2003. Asymmetry in the assembly of the RNAi enzyme complex. Cell 115:199-208
123. Seitz H, Zamore PD. 2006. Rethinking the microprocessor. Cell 125:827-29
124. Sethupathy P, Corda B, Hatzigeorgiou AG. 2006. TarBase: a comprehensive database of experimentally supported animal microRNA targets. RNA 12:192-97
125. Sethupathy P, Megraw M, Hatzigeorgiou AG. 2006. A guide through present computational approaches for the identification of mammalian microRNA targets. Nat. Methods 3:881-86
126. Si ML, Zhu S, Wu H, Lu Z, Wu F, Mo YY. 2006. miR-21-mediated tumor growth. Oncogene doi:10.1038/sj.onc.1210083
127. Sokol NS, Ambros V. 2005. Mesodermally expressed Drosophila microRNA-1 is regulated by Twist and is required in muscles during larval growth. Genes Dev. 19:2343-54
128. Song JJ, Smith SK, Hannon GJ, Joshua-Tor L. 2004. Crystal structure of Argonaute and its implications for RISC slicer activity. Science 305:1434-37
129. Sood P, Krek A, Zavolan M, Macino G, Rajewsky N. 2006. Cell-type-specific signatures of microRNAs on target mRNA expression. Proc. Natl. Acad. Sci. USA 103:2746-51
130. Soutschek J, Akinc A, Bramlage B, Charisse K, Constien R, et al. 2004. Therapeutic silencing of an endogenous gene by systemic administration of modified siRNAs. Nature 432:173-78
131. Takamizawa J, Konishi H, Yanagisawa K, Tomida S, Osada H, et al. 2004. Reduced expression of the let-7 microRNAs in human lung cancers in association with shortened postoperative survival. Cancer Res. 64:3753-56
132. Tam W, Ben-Yehuda D, Hayward WS. 1997. bic, a novel gene activated by proviral insertions in avian leukosis virus-induced lymphomas, is likely to function through its noncoding RNA. Mol. Cell. Biol. 17:1490-502
133. Tam W, Hughes SH, Hayward WS, Besmer P. 2002. Avian bic, a gene isolated from a common retroviral site in avian leukosis virus-induced lymphomas that encodes a non-coding RNA, cooperates with c-myc in lymphomagenesis and erythroleukemogenesis. J. Virol. 76:4275-86
134. Vo N, Klein ME, Varlamova O, Keller DM, Yamamoto T, et al. 2005. A cAMP-response element binding protein-induced microRNA regulates neuronal morphogenesis. Proc. Natl. Acad. Sci. USA 102:16426-31
135. Volinia S, Calin GA, Liu CG, Ambs S, Cimmino A, et al. 2006. A microRNA expression signature of human solid tumors defines cancer gene targets. Proc. Natl. Acad. Sci. USA 103:2257-61
136. Voorhoeve PM, le Sage C, Schrier M, Gillis AJ, Stoop H, et al. 2006. A genetic screen implicates miRNA-372 and miRNA-373 as oncogenes in testicular germ cell tumors. Cell 124:1169-81
137. Wang XJ, Reyes JL, Chua NH, Gaasterland T. 2004. Prediction and identification of Arabidopsis thaliana microRNAs and their mRNA targets. Genome Biol. 5:R65
138. Wienholds E, Kloosterman WP, Miska E, Alvarez-Saavedra E, Berezikov E, et al. 2005. MicroRNA expression in zebrafish embryonic development. Science 309:310-11
139. Wienholds E, Koudijs MJ, van Eeden FJ, Cuppen E, Plasterk RH. 2003. The microRNA-producing enzyme Dicer1 is essential for zebrafish development. Nat. Genet. 35:217-18
140. Wightman B, Ha I, Ruvkun G. 1993. Posttranscriptional regulation of the heterochronic gene lin-14 by lin-4 mediates temporal pattern formation in C. elegans. Cell 75:855-62
141. Xu P, Vernooy SY, Guo M, Hay BA. 2003. The Drosophila microRNA miR-14 suppresses cell death and is required for normal fat metabolism. Curr. Biol. 13:790-95
142. Yanaihara N, Caplen N, Bowman E, Seike M, Kumamoto K, et al. 2006. Unique microRNA molecular profiles in lung cancer diagnosis and prognosis. Cancer Cell 9:189-98
143. Yi R, O'Carroll D, Pasolli HA, Zhang Z, Dietrich FS, et al. 2006. Morphogenesis in skin is governed by discrete sets of differentially expressed microRNAs. Nat. Genet. 38:356-62
144. Yi R, Qin Y, Macara IG, Cullen BR. 2003. Exportin-5 mediates the nuclear export of premicroRNAs and short hairpin RNAs. Genes Dev. 17:3011-16
145. Zamore PD, Tuschl T, Sharp PA, Bartel DP. 2000. RNAi: double-stranded RNA directs the ATP-dependent cleavage of mRNA at 21 to 23 nucleotide intervals. Cell 101:25-33
146. Zeng Y, Cullen BR. 2004. Structural requirements for premicroRNA binding and nuclear export by Exportin 5. Nucleic Acids Res. 32:4776-85
147. Zeng Y, Yi R, Cullen BR. 2003. MicroRNAs and small interfering RNAs can inhibit mRNA expression by similar mechanisms. Proc. Natl. Acad. Sci. USA 100:9779-84
148. Zhang Y. 2005. miRU: an automated plant miRNA target prediction server. Nucleic Acids Res. 33:W701-4
149. Zhao Y, Samal E, Srivastava D. 2005. Serum response factor regulates a muscle-specific microRNA that targets Hand2 during cardiogenesis. Nature 436:214-20