Argonaute2 is the catalytic engine of mammalian RNAi

Science

Volumn 305, Issue 5689, 2004, Pages 1437-1441

  Liu, Jidong ^a   Carmell, Michelle A ^a,b   Rivas, Fabiola V ^a   Marsden, Carolyn G ^a   Thomson, J Michael ^c   Song, Ji Joon ^a   Hammond, Scott M ^c   Joshua Tor, Leemor ^a   Hannon, Gregory J ^a  

^a Simons Center for Quantitative Biology   (United States)

^b STONY BROOK UNIVERSITY   (United States)

^c UNIVERSITY OF NORTH CAROLINA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ARGONAUTE PROTEINS; CLEAVAGE ACTIVITIES; GENE SILENCING; RNA INTERFERENCE (RNAI);

BIOCHEMISTRY; CATALYSIS; CELLS; COMPLEXATION; GENES; GENETIC ENGINEERING; LIVING SYSTEMS STUDIES; MUTAGENESIS; PROTEINS;

RNA;

MESSENGER RNA; PROTEIN; RIBONUCLEASE H; RNA;

BIOLOGY; RNA;

ARTICLE; CATALYSIS; DNA MICROARRAY; EMBRYO; GENE; GENE EXPRESSION; GENE SILENCING; GENOTYPE; HUMAN; IN SITU HYBRIDIZATION; MAMMAL; MOUSE; NEURAL TUBE; NONHUMAN; PHENOTYPE; PRIORITY JOURNAL; PROTEIN EXPRESSION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA BINDING; RNA CLEAVAGE; RNA INTERFERENCE;

AMINO ACID SEQUENCE; ANIMALS; CATALYSIS; CELL LINE; CELLS, CULTURED; CENTRAL NERVOUS SYSTEM; EMBRYONIC AND FETAL DEVELOPMENT; GENE EXPRESSION PROFILING; GENE EXPRESSION REGULATION, DEVELOPMENTAL; HUMANS; IN SITU HYBRIDIZATION; MICE; MICRORNAS; MOLECULAR SEQUENCE DATA; MUTAGENESIS, INSERTIONAL; OLIGONUCLEOTIDE ARRAY SEQUENCE ANALYSIS; PEPTIDE INITIATION FACTORS; POINT MUTATION; RNA INTERFERENCE; RNA, DOUBLE-STRANDED; RNA, MESSENGER; RNA, SMALL INTERFERING; RNA-INDUCED SILENCING COMPLEX;

MAMMALIA;

EID: 4444368187     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.1102513     Document Type: Article

References (45)

1. G. J. Hannon, Nature 418, 244 (2002).
2. A. Fire et al., Nature 391, 806 (1998).
3. G. Hutvagner, P. D. Zamore, Curr. Opin. Genet. Dev. 12, 225 (2002).
4. A. Hamilton, O. Voinnet, L. Chappell, D. Baulcombe, EMBO J. 21, 4671 (2002).
5. E. Bernstein, A. A. Caudy, S. M. Hammond, G. J. Hannon, Nature 409, 363 (2001).
6. Y. Lee et al., Nature 425, 415 (2003).
7. S. M. Hammond, E. Bernstein, D. Beach, G. J. Hannon, Nature 404, 293 (2000).
8. M. F. Mette, W. Aufsatz, J. van der Winden, M. A. Matzke, A. J. Matzke, EMBO J. 19, 5194 (2000).
9. I. M. Hall et al., Science 297, 2232 (2002).
10. T. Volpe et al., Science 297, 1833 (2002).
11. M. Pal-Bhadra, U. Bhadra, J. A. Birchler, Mol. Cell 9, 315 (2002).
12. P. H. Olsen, V. Ambros, Dev. Biol. 216, 671 (1999).
13. D. P. Bartel, Cell 116, 281 (2004).
14. T. Tuschl, P. D. Zamore, R. Lehmann, D. P. Bartel, P. A. Sharp, Genes Dev. 13, 3191 (1999).
15. P. D. Zamore, T. Tuschl, P. A. Sharp, D. P. Bartel, Cell 101, 25 (2000).
16. S. M. Elbashir, J. Martinez, A. Patkaniowska, W. Lendeckel, T. Tuschl, EMBO J. 20, 6877 (2001).
17. J. Martinez, A. Patkaniowska, H. Urlaub, R. Luhrmann, T. Tuschl, Cell 110, 563 (2002).
18. G. Hutvagner, P. D. Zamore, Science 297, 2056 (2002).
19. S. M. Hammond, S. Boettcher, A. A. Caudy, R. Kobayashi, G. J. Hannon, Science 293, 1146 (2001).
20. M. A. Carmell, G. J. Hannon, Nature Struct. Mol. Biol. 11, 214 (2004).
21. L. Cerutti, N. Mian, A. Bateman, Trends Biochem. Sci. 25, 481 (2000).
22. H. Tabara et al., Cell 99, 123 (1999).
23. M. A. Carmell, Z. Xuan, M. Q. Zhang, G. J. Hannon, Genes Dev. 16, 2733 (2002).
24. A. A. Caudy, M. Myers, G. J. Hannon, S. M. Hammond, Genes Dev. 16, 2491 (2002).
25. K. Okamura, A. Ishizuka, H. Siomi, M. C. Siomi, Genes Dev. 18, 1655 (2004).
26. B. Zheng, A. A. Mills, A. Bradley, Nucleic Acids Res. 27, 2354 (1999).
27. S. J. Conway, A. Kruzynska-Frejtag, P. L. Kneer, M. Machnicki, S. V. Koushik, Genesis 35, 1 (2003).
28. W. Deng, H. Lin, Dev. Cell 2, 819 (2002).
29. S. Kuramochi-Miyagawa et al., Development 131, 839 (2004).
30. T. Sasaki, A. Shiohama, S. Minoshima, N. Shimizu, Genomics 82, 323 (2003).
31. S. Yekta, I. H. Shih, D. P. Bartel, Science 304, 594 (2004).
32. J. G. Doench, C. P. Petersen, P. A. Sharp, Genes Dev. 17, 438 (2003).
33. M. Kiriakidou et al., Genes Dev. 18, 1165 (2004).
34. A. Nykanen, B. Haley, P. D. Zamore, Cell 107, 309 (2001).
35. J. W. Pham, J. L. Pellino, Y. S. Lee, R. W. Carthew, E. J. Sontheimer, Cell 117, 83 (2004).
36. Y. Tomari et al., Cell 116, 831 (2004).
37. J.-J. Song et al., Science 305, 1434 (2004). Published online 29 July 2004; 10.1126/science.1102514.
38. B. R. Chapados et al., J. Mol. Biol. 307, 541 (2001).
39. W. Yang, T. A. Steitz, Structure 3, 131 (1995).
40. D. S. Schwarz, Y. Tomari, P. D. Zamore, Curr. Biol. 14, 787 (2004).
41. R. F. Ketting, T. H. Haverkamp, H. G. van Luenen, R. H. Plasterk, Cell 99, 133 (1999).
42. T. Sijen, R. H. Plasterk, Nature 426, 310 (2003).
43. E. Sarot, G. Payen-Groschene, A. Bucheton, A. Pelisson, Genetics 166, 1313 (2004).
44. Materials and methods are available as supporting material on Science Online.
45. The authors thank members of the Hannon lab for helpful discussions, Alea Mills for advice on ES cell work and for providing the library of targeting constructs, Sang Yong Kim for generating chimeras, Kathryn Anderson for insightful discussions and advice, and Phil Sharp for providing the CXCR4 constructs. M.C. is supported by the U.S. Army Breast Cancer Research Program, F.V.R. by the Jane Coffin Childs Memorial Fund, and J.S. by a Bristol Myers Squibb predoctoral fellowship. S.M.H. is a General Motors Cancer Research Foundation Scholar. This work was supported in part by grants from NIH (L.J. and G.J.H.).