Exportin 5 is a RanGTP-dependent dsRNA-binding protein that mediates nuclear export of pre-miRNAs

RNA

Volumn 10, Issue 2, 2004, Pages 185-191

  Bohnsack, Markus T ^a   Czaplinski, Kevin ^b   Görlich, Dirk ^a,c  

^a P   (Germany)

^b EUROPEAN MOLECULAR BIOLOGY LABORATORY   (Germany)

^c INF 282   (Germany)

Author keywords

Exportin; miRNA; Nuclear transport; Silencing; Translation regulation; tRNA

Indexed keywords

COMPLEMENTARY DNA; DOUBLE STRANDED RNA; EXPORTIN 5; GUANOSINE TRIPHOSPHATASE; GUANOSINE TRIPHOSPHATE; MESSENGER RNA; MICRORNA; PHOSPHORUS 32; PROTEIN ANTIBODY; RAN PROTEIN; RNA BINDING PROTEIN; TRANSFER RNA; UNCLASSIFIED DRUG; URIDINE TRIPHOSPHATE;

ADENOVIRUS; ANIMAL CELL; ARTICLE; CELL NUCLEUS MEMBRANE; DROSOPHILA; EUKARYOTE; GENE EXPRESSION REGULATION; GENE SILENCING; MOLECULAR CLONING; NONHUMAN; NUCLEOCYTOPLASMIC TRANSPORT; NUCLEOTIDE SEQUENCE; OOCYTE; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN TRANSPORT; RNA BINDING; RNA TRANSLATION; RNA TRANSPORT; SACCHAROMYCES CEREVISIAE; XENOPUS;

ACTIVE TRANSPORT, CELL NUCLEUS; ANIMALS; CELL NUCLEUS; DROSOPHILA; HUMANS; KARYOPHERINS; MICRORNAS; MOLECULAR SEQUENCE DATA; PROTEIN BINDING; RAN GTP-BINDING PROTEIN; RNA PRECURSORS; RNA, DOUBLE-STRANDED; RNA, TRANSFER; XENOPUS;

ADENOVIRIDAE; ANIMALIA; EUKARYOTA; SACCHAROMYCES; SACCHAROMYCES CEREVISIAE;

EID: 1642499415     PISSN: 13558382     EISSN: None     Source Type: Journal    
DOI: 10.1261/rna.5167604     Document Type: Article

References (40)

1. Ambros, V., Bartel, B., Bartel, D.P., Burge, C.B., Carrington, J.C., Chen, X., Dreyfuss, G., Eddy, S.R., Griffiths-Jones, S., Marshall, M., et al. 2003. A uniform system for microRNA annotation. RNA 9: 277-279.
2. Arts, G.J., Fornerod, M., and Mattaj, I.W. 1998a. Identification of a nuclear export receptor for tRNA. Curr. Biol. 8: 305-314.
3. Arts, G.J., Kuersten, S., Romby, P., Ehresmann, B., and Mattaj, I.W. 1998b. The role of exportin-t in selective nuclear export of mature tRNAs. EMBO J. 17: 7430-7441.
4. Bohnsack, M.T., Regener, K., Schwappach, B., Saffrich, R., Paraskeva, E., Hartmann, E., and Görlich, D. 2002. Exp5 exports eEF1A via tRNA from nuclei and synergizes with other transport pathways to confine translation to the cytoplasm. EMBO J. 21: 6205-6215.
5. Bollman, K.M., Aukerman, M.J., Park, M.Y., Hunter, C., Berardini, T.Z., and Poethig, R.S. 2003. HASTY, the Arabidopsis ortholog of exportin 5/MSN5, regulates phase change and morphogenesis. Development 130: 1493-1504.
6. Calado, A., Treichel, N., Muller, E.C., Otto, A., and Kutay, U. 2002. Exportin-5-mediated nuclear export of eukaryotic elongation factor 1A and tRNA. EMBO J. 21: 6216-6224.
7. Carrington, J.C. and Ambros, V. 2003. Role of microRNAs in plant and animal development. Science 301: 336-338.
8. Cerutti, H. 2003. RNA interference: Traveling in the cell and gaining functions? Trends Genet. 19: 39-46.
9. Cullen, B.R. 2002. RNA interference: Antiviral defense and genetic tool. Nat. Immunol. 3: 597-599.
10. Doench, J.G., Petersen, C.P., and Sharp, P.A. 2003. siRNAs can function as miRNAs. Genes & Dev. 17: 438-442.
11. Fornerod, M., Ohno, M., Yoshida, M., and Mattaj, I.W. 1997. Crm1 is an export receptor for leucine rich nuclear export signals. Cell 90: 1051-1060.
12. Görlich, D. and Kutay, U. 1999. Transport between the cell nucleus and the cytoplasm. Annu. Rev. Cell Dev. Biol. 15: 607-660.
13. Grüter, P., Tabernero, C., von Kobbe, C., Schmitt, C., Saavedra, C., Bachi, A., Wilm, M., Felber, B.K., and Izaurralde, E. 1998. TAP, the human homolog of Mex67p, mediates CTE-dependent RNA export from the nucleus. Mol. Cell 1: 649-659.
14. Gwizdek, C., Bertrand, E., Dargemont, C., Lefebvre, J.C., Blanchard, J.M., Singer, R.H., and Doglio, A. 2001. Terminal minihelix, a novel RNA motif that directs polymerase III transcripts to the cell cytoplasm. Terminal minihelix and RNA export. J. Biol. Chem. 276: 25910-25918.
15. Gwizdek, C., Ossareh-Nazari, B., Brownawell, A.M., Doglio, A., Bertrand, E., Macara, I.G., and Dargemont, C. 2003. Exportin-5 mediates nuclear export of minihelix-containing RNAs. J. Biol. Chem. 278: 5505-5508.
16. Hutvagner, G. and Zamore, P.D. 2002a. A microRNA in a multiple-turnover RNAi enzyme complex. Science 297: 2056-2060.
17. -. 2002b. RNAi: Nature abhors a double-strand. Curr. Opin. Genet. Dev. 12: 225-232.
18. Izaurralde, E., Kutay, U., von Kobbe, C., Mattaj, I.W., and Görlich, D. 1997. The asymmetric distribution of the constituents of the Ran system is essential for transport into and out of the nucleus. EMBO J. 16: 6535-6547.
19. Jarmolowski, A., Boelens, W.C., Izaurralde, E., and Mattaj, I.W. 1994. Nuclear export of different classes of RNA is mediated by specific factors. J. Cell Biol. 124: 627-635.
20. Kawasaki, H. and Taira, K. 2003. Hes1 is a target of microRNA-23 during retinoic-acid-induced neuronal differentiation of NT2 cells. Nature 423: 838-842.
21. Kutay, U., Bischoff, F.R., Kostka, S., Kraft, R., and Görlich, D. 1997. Export of importin α from the nucleus is mediated by a specific nuclear transport factor. Cell 90: 1061-1071.
22. Kutay, U., Lipowsky, G., Izaurralde, E., Bischoff, F.R., Schwarzmaier, P., Hartmann, E., and Görlich, D. 1998. Identification of a tRNA-specific nuclear export receptor. Mol. Cell 1: 359-369.
23. Lagos-Quintana, M., Rauhut, R., Lendeckel, W., and Tuschl, T. 2001. Identification of novel genes coding for small expressed RNAs. Science 294: 853-858.
24. Lee, R.C., Feinbaum, R.L., and Ambros, V. 1993. The C. elegans heterochronic gene lin-4 encodes small RNAs with antisense complementarity to lin-14. Cell 75: 843-854.
25. Lee, Y., Jeon, K., Lee, J.T., Kim, S., and Kim, V.N. 2002. MicroRNA maturation: stepwise processing and subcellular localization. EMBO J. 21: 4663-4670.
26. Lee, Y., Ahn, C., Han, J., Choi, H., Kim, J., Yim, J., Lee, J., Provost, P., Radmark, O., Kim, S., et al. 2003. The nuclear RNase III Drosha initiates microRNA processing. Nature 425: 415-419.
27. Macara, I.G. 2001. Transport into and out of the nucleus. Microbiol. Mol. Biol. Rev. 65: 570-594, table of contents.
28. Mattaj, I.W. and Englmeier, L. 1998. Nucleocytoplasmic transport: the soluble phase. Annu. Rev. Biochem. 67: 265-306.
29. Mourelatos, Z., Dostie, J., Paushkin, S., Sharma, A., Charroux, B., Abel, L., Rappsilber, J., Mann, M., and Dreyfuss, G. 2002. miRNPs: A novel class of ribonucleoproteins containing numerous micro-RNAs. Genes & Dev. 16: 720-728.
30. Ohno, M., Segref, A., Bachi, A., Wilm, M., and Mattaj, I.W. 2000. PHAX, a mediator of U snRNA nuclear export whose activity is regulated by phosphorylation. Cell 101: 187-198.
31. Pasquinelli, A.E. and Ruvkun, G. 2002. Control of developmental timing by microRNAs and their targets. Annu. Rev. Cell Dev. Biol. 18: 495-513.
32. Reinhart, B.J., Slack, F.J., Basson, M., Pasquinelli, A.E., Bettinger, J.C., Rougvie, A.E., Horvitz, H.R., and Ruvkun, G. 2000. The 21-nucleotide let-7 RNA regulates developmental timing in Caenorhabditis elegans. Nature 403: 901-906.
33. Saunders, L.R. and Barber, G.N. 2003. The dsRNA binding protein family: Critical roles, diverse cellular functions. FASEB J. 17: 961-983.
34. + RNA and nuclear pores. EMBO J. 16: 3256-3271.
35. Snay-Hodge, C.A., Colot, H.V., Goldstein, A.L., and Cole, C.N. 1998. Dbp5p/Rat8p is a yeast nuclear pore-associated DEAD-box protein essential for RNA export. EMBO J. 17: 2663-2676.
36. + RNA export. EMBO J. 17: 2651-2662.
37. Weis, K. 2002. Nucleocytoplasmic transport: Cargo trafficking across the border. Curr. Opin. Cell Biol. 14: 328-335.
38. Wozniak, R.W., Rout, M.P., and Aitchison, J.D. 1998. Karyopherins and kissing cousins. Trends Cell Biol. 8: 184-188.
39. Zeng, Y. and Cullen, B.R. 2002. RNA interference in human cells is restricted to the cytoplasm. RNA 8: 855-860.
40. Zeng, Y., Yi, R., and Cullen, B.R. 2003. MicroRNAs and small interfering RNAs can inhibit mRNA expression by similar mechanisms. Proc. Natl. Acad. Sci. 5: 5.