Chimeric Gene Library Construction by a Simple and Highly Versatile Method Using Recombination-Dependent Exponential Amplification

Biotechnology Progress

Volumn 19, Issue 5, 2003, Pages 1460-1467

  Ikeuchi, Akinori ^a   Kawarasaki, Yasuaki ^a,c   Shinbata, Tomoya ^b   Yamane, Tsuneo ^a  

^a NAGOYA UNIVERSITY   (Japan)

^b Kanagawa 243 0041   (Japan)

^c UNIVERSITY OF TEXAS AT AUSTIN   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANNEALING; ENZYMES; FLUORESCENCE; GENES;

CHAIN REACTIONS;

BIOTECHNOLOGY;

BACTERIAL PROTEINS; DIRECTED MOLECULAR EVOLUTION; FEASIBILITY STUDIES; GENE AMPLIFICATION; GENE LIBRARY; GREEN FLUORESCENT PROTEINS; LUMINESCENT PROTEINS; POLYMERASE CHAIN REACTION; RECOMBINANT FUSION PROTEINS; RECOMBINANT PROTEINS; RECOMBINATION, GENETIC;

EID: 0141908152     PISSN: 87567938     EISSN: None     Source Type: Journal    
DOI: 10.1021/bp034029t     Document Type: Article

References (11)

1. Abecassis, V.; Pompon, D.; Truan. G. High Efficiency Family Shuffling Based on Multi-step PCR and in Vivo DNA Recom-bination in Yeast: Statistical and Functional Analysis of a Combinatorial Library between Human P450 1A1 and 1A2. Nucleic Acid Res. 2000, 28, e88.
2. Christians, F. C.; Scapozza, L.; Crameri, A.; Folkers, G.; Stemmer, W. P. C. Directed Evolution of Thymidine Kinase for AZT Phosphorylation using DNA Family Shuffling. Nat. Biotechnol. 1999, 17, 259-264.
3. Coco, W. M.; Levinson, W. E.; Crist, M. J.; Hektor, H. J.; Darzins, A.; Pienkos, P. T.; Squires, C. H.; Monticello, D. J. DNA Snuffling Method for Generating Highly Recombined Genes and Evolved Enzymes. Nat. Biotechnol. 2001, 19, 354-359.
4. Crameri, A.; Raillard, S.-A.; Bermudez, E.; Stemmer, W. P. C. DNA Shuffling of a Family of Genes from Diverse Species Accelerates Direct Evolution. Nature 1998, 391, 288-291.
5. Kawarasaki, Y.; Sasaki, Y.; Ikeuchi, A.; Yamamoto, S.; Yamane, T. A Method for Functional Mapping of Protein-Protein Binding Domain by Preferential Amplification of the Shortest Amplicon Using PCR. Anal. Biochem. 2002, 303, 34-41.
6. Kikuchi, M.; Ohnishi, K.; Harayama, S. Novel Family Shuffling Methods for the in vitro Evolution of Enzymes. Gene 1999, 236, 159-167.
7. Kumamaru, T.; Suenaga, H.; Mitsuoka, M.; Watanabe, T.; Furukawa, K. Enhanced Degradation of Polychlorinated Biphenyls by Directed Evolution of Biphenyl Dioxygenase. Nat. Biotechnol. 1998, 16, 663-666.
8. Ness, J. R.; Welch, M.; Giver, L.; Bueno, M.; Cherry, J. R.; Borchert, T.; Stemmer, W. P. C.; Minshull, J. DNA Shuffling of Subgenomic Sequences of Subtilisin. Nat. Biotechnol. 1999, 17, 893-896.
9. Raillard, S.-A.; Krebber, A.; Chen, Y.; Ness, J. E.; Bermudez, E.; Trinidad, R.; Fullem, R.; Davis, C.; Welch, M.; Seffernick, J.; Wackett, L. P.; Stemmer, W. P. C.; Minshull, J. Novel Enzyme Activities and Functional Plasticity Revealed by Recombining Highly Homologous Enzymes. Chem. Biol. 2001 8, 891-898.
10. Stemmer, W. P. C. DNA Shuffling by Random Fragmentation and Reassembly: In Vitro Recombination for Molecular Evolution. Proc. Natl. Acad. Sci. U.S.A. 1994, 91, 10747-10751.
11. Zhao, H.; Giver, L.; Shao, Z.; Affholter, J. A.; Arnold, F. H. Molecular Evolution by Staggered Extension Process (StEP) in Vitro recombination. Nat. Biotechnol. 1998, 16, 258-261.