Directed evolution of a glycosynthase from Agrobacterium sp. increases its catalytic activity dramatically and expands its substrate repertoire

Journal of Biological Chemistry

Volumn 279, Issue 41, 2004, Pages 42787-42793

  Kim, Young Wan ^a   Lee, Seung Seo ^a   Warren, R Antony J ^a   Withers, Stephen G ^a  

^a UNIVERSITY OF BRITISH COLUMBIA   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

BIODIVERSITY; CATALYSIS; CHEMICAL BONDS; CONFORMATIONS; ENZYMES; MUTAGENESIS; SUBSTRATES; SUGAR (SUCROSE);

AGROBACTERIUM SP.; HOMOLOGY; MUTANTS; TRANSFORMANTS;

BACTERIA;

4 NITROPHENYL BETA DEXTRO GLUCOPYRANOSIDE; ALPHA DEXTRO GALACTOPYRANOSYL FLUORIDE; ALPHA GLYCOSYLFLUORIDE DERIVATIVE; BACTERIAL ENZYME; BETA GLUCOSIDASE; BETA GLYCOSIDASE; CARBOHYDRATE DERIVATIVE; CELLULASE; FLUORIDE; GLUTAMIC ACID; GLYCOSIDASE; GLYCOSIDE; GLYCOSYNTHASE; HYDROXYL GROUP; MUTANT PROTEIN; PYRANOSIDE; TRYPTOPHAN; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; ARTICLE; CATALYSIS; CATALYST; CHEMICAL BOND; CONFORMATIONAL TRANSITION; CONTROLLED STUDY; DIRECTED MOLECULAR EVOLUTION; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME ASSAY; ENZYME MECHANISM; ENZYME MODIFICATION; ENZYME STRUCTURE; ENZYME SUBSTRATE; GENE LIBRARY; GENE MUTATION; MOLECULAR INTERACTION; MOLECULAR MODEL; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; RHIZOBIUM; SEQUENCE HOMOLOGY; SITE DIRECTED MUTAGENESIS; STRUCTURE ANALYSIS; TECHNIQUE;

BETA-GLUCOSIDASE; BINDING SITES; BIOCHEMISTRY; DIRECTED MOLECULAR EVOLUTION; DOSE-RESPONSE RELATIONSHIP, DRUG; DOXORUBICIN; GENE LIBRARY; GENETIC VECTORS; GLUTAMIC ACID; KINETICS; MODELS, CHEMICAL; MODELS, MOLECULAR; MUTAGENESIS; MUTATION; PROTEIN BINDING; PROTEIN TRANSPORT; RHIZOBIUM; SEQUENCE ANALYSIS, DNA; SUBSTRATE SPECIFICITY; TRYPTOPHAN;

AGROBACTERIUM; AGROBACTERIUM SP.; BACTERIA (MICROORGANISMS); NEGIBACTERIA;

EID: 5644249284     PISSN: 00219258     EISSN: None     Source Type: Journal    
DOI: 10.1074/jbc.M406890200     Document Type: Article

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