An efficient method for creation and functional analysis of libraries of hybrid type I polyketide synthases

Protein Engineering, Design and Selection

Volumn 17, Issue 3, 2004, Pages 277-284

  Kim, Beom Seok ^a   Sherman, David H ^b   Reynolds, Kevin A ^a  

^a VIRGINIA COMMONWEALTH UNIVERSITY   (United States)

^b UNIVERSITY OF MICHIGAN   (United States)

Author keywords

Acyl carrier protein; Acyltransferase; Erythromycin; Hybrid PKS; Pikromycin; Type I polyketide synthase

Indexed keywords

DNA; HYBRID PROTEIN; PEPTIDE LIBRARY; PIKROMYCIN; POLYKETIDE SYNTHASE; POLYKETIDE SYNTHASE I; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; ARTICLE; BACTERIUM MUTANT; BIOASSAY; CHIMERA; DELETION MUTANT; GENE LIBRARY; GENETIC COMPLEMENTATION; NONHUMAN; PLASMID; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN EXPRESSION; SEQUENCE ANALYSIS; STREPTOMYCES VENEZUELAE;

ACYLTRANSFERASES; AMINO ACID SEQUENCE; BACTERIAL PROTEINS; CATALYTIC DOMAIN; DIRECTED MOLECULAR EVOLUTION; ESCHERICHIA COLI; GENE DELETION; GENETIC COMPLEMENTATION TEST; GENETIC MARKERS; MACROLIDES; MOLECULAR STRUCTURE; PEPTIDE LIBRARY; PLASMIDS; POLYKETIDE SYNTHASES; PROTEIN STRUCTURE, TERTIARY; RECOMBINANT FUSION PROTEINS; RECOMBINATION, GENETIC; STREPTOMYCES;

ACTINOBACTERIA (CLASS); BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; STREPTOMYCES; STREPTOMYCES VENEZUELAE; UNCULTURED ACTINOMYCETE;

EID: 2942674459     PISSN: 17410126     EISSN: None     Source Type: Journal    
DOI: 10.1093/protein/gzh032     Document Type: Article

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