Complete reversal of enantioselectivity of an enzyme-catalyzed reaction by directed evolution

Chemical Communications

Volumn 1, Issue 24, 2001, Pages 2664-2665

  Zha, Dongxing ^a   Wilensek, Stephanie ^a   Hermes, Markus ^a   Jaeger, Karl Erich ^b   Reetz, Manfred T ^a  

^a MAX PLANCK INSTITUT FÜR KOHLENFORSCHUNG   (Germany)

^b RUHR UNIVERSITY BOCHUM   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

DNA; ENZYME; ESTER; TRIACYLGLYCEROL LIPASE;

ARTICLE; CATALYSIS; CHIRALITY; ENANTIOMER; ENZYME KINETICS; ENZYME MECHANISM; HYDROLYSIS; MUTATION RATE; POLYMERASE CHAIN REACTION; STEREOCHEMISTRY;

EID: 0035930698     PISSN: 13597345     EISSN: 1364548X     Source Type: Journal    
DOI: 10.1039/b109913m     Document Type: Article

References (22)

1. D. W. Leung, E. Chen and D. V. Goeddel, Technique (Philadelphia), 1989, 1, 11-15;
2. R. C. Cadwell and G. F. Joyce, PCR Methods Appl., 1994, 3, S136-S140;
3. B. Steipe, in Combinatorial Chemistry in Biology, ed. M. Famulok, E.-L. Winnacker and C.-H. Wong, Springer, Berlin, 1999, vol. 243, pp. 55-86;
4. J. D. Sutherland, Curr. Opin. Chem. Biol., 2000, 4, 263-269.
5. F. H. Arnold, Acc. Chem. Res., 1998, 31, 125;
6. F. H. Arnold, Nature (London), 2001, 409, 253-257.
7. W. P. C. Stemmer, Nature (London), 1994, 370, 389-391;
8. A. Crameri, S.-A. Raillard, E. Bermudez and W. P. C. Stemmer, Nature (London), 1998, 391, 288-291.
9. M. T. Reetz, A. Zonta, K. Schimossek, K. Liebeton and K.-E. Jaeger, Angew. Chem., 1997, 109, 2961-2963;
10. Angew. Chem., Int. Ed. Engl., 1997, 36, 2830-2832;
11. K. Liebeton, A. Zonta, K. Schimossek, M. Nardini, D. Lang, B. W. Dijkstra, M. T. Reetz and K. E. Jaeger, Chem. Biol., 2000, 7, 709-718.
12. M. T. Reetz and K. E. Jaeger, Chem. Eur. J., 2000, 6, 407-412;
13. M. T. Reetz, Pure Appl. Chem., 2000, 72, 1615-1622.
14. A. Crameri and W. P. C. Stemmer, BioTechniques, 1995, 18, 194-196.
15. M. T. Reetz, S. Wilensek, D. Zha and K.-E. Jaeger, Angew. Chem., 2001, 113, 3701-3703;
16. Angew. Chem., Int. Ed., 2001, 40, 3589-3591.
17. O. May, P. T. Nguyen and F. H. Arnold, Nature Biotechnol., 2000, 18, 317-320.
18. M. T. Reetz, Angew. Chem., 2001, 113, 292-320;
19. Angew. Chem., Int. Ed., 2001, 40, 284-310.
20. S. Fong, T. D. Machajewski, C. C. Mak and C.-H. Wong, Chem. Biol., 2000, 7, 873-883;
21. K. M. Koeller and C.-H. Wong, Nature (London), 2001, 409, 232-240.
22. M. Nardini, D. A. Lang, K. Liebeton, K.-E. Jaeger and B. W. Dijkstra, J. Biol. Chem., 2000, 275, 31219-31225