Increasing the synthetic performance of penicillin acylase PAS2 by structure-inspired semi-random mutagenesis

Protein Engineering, Design and Selection

Volumn 17, Issue 7, 2004, Pages 571-579

  Gabor, Esther M ^a   Janssen, Dick B ^a  

^a UNIVERSITY OF GRONINGEN   (Netherlands)

Author keywords

lactam antibiotics; Penicillin acylase; Semi random mutagenesis; Synthesis

Indexed keywords

AMINO ACIDS; BIOCATALYSTS; DERIVATIVES; ENZYMES;

6-AMINOPENCILLANIC ACID; MUTANTS; PENICILLIN;

MUTAGENESIS;

6 AMINOPENICILLANIC ACID; 7 AMINODESACETOXYCEPHALOSPORANIC ACID; AMIDASE; AMPICILLIN; BETA LACTAM ANTIBIOTIC; CEFALEXIN; CEPHALOSPORANIC ACID DERIVATIVE; PENICILLIN ACYLASE PAS2; PHENYLGLYCINE; UNCLASSIFIED DRUG;

ARTICLE; BIOCATALYST; ENZYME ACTIVE SITE; ENZYME PURIFICATION; ENZYME SUBSTRATE; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HYDROLYSIS; MATHEMATICAL MODEL; MUTAGENESIS; PRIORITY JOURNAL; PROTEIN STRUCTURE; WILD TYPE;

BETA-LACTAMS; CATALYSIS; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; DOSE-RESPONSE RELATIONSHIP, DRUG; ESCHERICHIA COLI; GENETIC TECHNIQUES; GLYCINE; HYDROLYSIS; KINETICS; MODELS, CHEMICAL; MUTAGENESIS; MUTATION; PENICILLANIC ACID; PENICILLIN AMIDASE; PLASMIDS; PROTEIN CONFORMATION; PROTEIN ENGINEERING; TIME FACTORS;

EID: 9644252999     PISSN: 17410126     EISSN: None     Source Type: Journal    
DOI: 10.1093/protein/gzh070     Document Type: Article

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