Discovery and directed evolution of a glyphosate tolerance gene

Science

Volumn 304, Issue 5674, 2004, Pages 1151-1154

  Castle, Linda A ^a   Siehl, Daniel L ^a   Gorton, Rebecca ^a   Patten, Phillip A ^b   Chen, Yong Hong ^b   Bertain, Sean ^a   Cho, Hyeon Je ^a   Duck, Nicholas ^c,d   Wong, James ^c   Liu, Donglong ^c   Lassner, Michael W ^a  

^a Verdia Inc   (United States)

^b MAXYGEN INC   (United States)

^c PIONEER HI BRED INTERNATIONAL INC   (United States)

^d Athenix Corp   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ACETYLATION; CROPS; ENZYMES; HERBICIDES; TOBACCO;

TOLERANCE GENE; TRANSGENIC ORGANISMS;

GENES;

ACYLTRANSFERASE; DNA; GLYPHOSATE; GLYPHOSATE N ACETYLTRANSFERASE; HERBICIDE; UNCLASSIFIED DRUG;

MEDICINE;

AMINO ACID SEQUENCE; ARABIDOPSIS; ARTICLE; BACILLUS LICHENIFORMIS; DETOXIFICATION; ENZYME ACTIVITY; ESCHERICHIA COLI; EVOLUTION; GENETIC VARIABILITY; GENETICALLY MODIFIED CROP; HERBICIDE RESISTANCE; HERBICIDE TOLERANCE; MAIZE; MASS SPECTROMETRY; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; TOBACCO;

ACETYLATION; ACETYLTRANSFERASES; AMINO ACID SEQUENCE; BACILLUS; CATALYSIS; DIRECTED MOLECULAR EVOLUTION; DNA SHUFFLING; DRUG RESISTANCE; ESCHERICHIA COLI; GENE LIBRARY; GLYCINE; HERBICIDES; KINETICS; MOLECULAR SEQUENCE DATA; MUTAGENESIS; PLANTS, GENETICALLY MODIFIED; RECOMBINANT PROTEINS; RECOMBINATION, GENETIC; TOBACCO; TRANSFORMATION, GENETIC; VARIATION (GENETICS); ZEA MAYS;

ARABIDOPSIS; BACILLUS LICHENIFORMIS; ESCHERICHIA COLI; NICOTIANA TABACUM; ZEA MAYS;

EID: 2442668927     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.1096770     Document Type: Article

References (27)

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27. We thank Y. Chen, T. Chen, L. Giver, C. Ivy, C. Krebber, G. Wu, J. Wilkinson, A. Wong, N. Trinh, A. Madrigal, A. Umthun, P. Olsen, and W. Mehre for excellent technical assistance; J. Minshull, S. Govindarajan, R. Emig, and the Maxygen bioinformatics team; D. Dagarin and A. Wadley at Byotix for the glyphosate tolerance tests with gat tobacco.