Construction of stabilized proteins by combinatorial consensus mutagenesis

Protein Engineering, Design and Selection

Volumn 17, Issue 11, 2004, Pages 787-793

  Amin, N ^a   Liu, A D ^a   Ramer, S ^a   Aehle, W ^b   Meijer, D ^b   Metin, M ^b   Wong, S ^a   Gualfetti, P ^a   Schellenberger, V ^a  

^a GENENCOR INTERNATIONAL INC   (United States)

^b Genencor International BV   (Netherlands)

Author keywords

Combinatorial mutagenesis; Consensus mutation; Lactamase; Proteolysis; Thermostability

Indexed keywords

GENES; MUTAGENESIS; STABILIZATION; THERMODYNAMIC STABILITY;

Β-LACTAMASE (BLA); COMBINATORIAL CONSENSUS MUTAGENESIS (CCM); MUTATIONS; PROTEOLYTIC STABILITY;

PROTEINS;

BACTERIAL PROTEIN; BETA LACTAMASE; DNA; OLIGONUCLEOTIDE; PROTEINASE;

AMINO ACID SEQUENCE; ARTICLE; COMBINATORIAL MUTAGENESIS; CONSENSUS MUTATION; ENTEROBACTER CLOACAE; ENZYME ACTIVITY; ESCHERICHIA COLI; GENE MUTATION; MUTAGENESIS; NONHUMAN; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN STABILITY; THERMOSTABILITY;

AMINO ACID SEQUENCE; BETA-LACTAMASES; COMBINATORIAL CHEMISTRY TECHNIQUES; ENTEROBACTER CLOACAE; ENZYME STABILITY; GENE LIBRARY; HEAT; MOLECULAR SEQUENCE DATA; MUTAGENESIS; MUTATION; PROTEIN ENGINEERING; PROTEINS;

ENTEROBACTER CLOACAE;

EID: 14644390952     PISSN: 17410126     EISSN: None     Source Type: Journal    
DOI: 10.1093/protein/gzh091     Document Type: Article

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