Using small molecules to study big questions in cellular microbiology

Cellular Microbiology

Volumn 4, Issue 8, 2002, Pages 471-482

  Ward, Gary E ^a   Carey, Kimberly L ^a   Westwood, Nicholas J ^b,c  

^a UNIVERSITY OF VERMONT COLLEGE OF MEDICINE   (United States)

^b HARVARD MEDICAL SCHOOL   (United States)

^c UNIVERSITY OF ST ANDREWS   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIINFECTIVE AGENT; COMPLEMENTARY DNA;

ANTIMICROBIAL ACTIVITY; CHEMICAL STRUCTURE; CONTROLLED STUDY; CYTOLOGY; DRUG SCREENING; HOST PATHOGEN INTERACTION; LEARNING; MEDICAL TECHNOLOGY; MICROBIAL GENETICS; MICROBIOLOGY; MOLECULAR BIOLOGY; NONHUMAN; PHENOTYPE; PRIORITY JOURNAL; PROTEIN TARGETING; REVIEW; SYNTHESIS;

ANIMALS; DRUG DESIGN; DRUG EVALUATION, PRECLINICAL; MICROBIOLOGICAL TECHNIQUES; MICROBIOLOGY; MOLECULAR STRUCTURE;

EID: 0036669959     PISSN: 14625814     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1462-5822.2002.00205.x     Document Type: Review

References (118)

1. Database of molecular probes set to boost molecular genetics
2. Solutions for library encoding to create collections of discrete compounds
3. Peptide and peptidomimetic libraries. Molecular diversity and drug design
4. Characterization and intracellular trafficking pattern of vacuoles containing Chlamydia pneumoniae in human epithelial cells
5. Unnatural ligands for engineered proteins: New tools for chemical genetics
6. Lytic cycle of Toxoplasma gondii
7. Cellular screening assays using fluorescence microscopy
8. Novel antimicrobial compounds identified using synthetic combinatorial library technology
9. Bradyzoite-specific genes
10. Discovery and development of the novel potent orally active thrombin inhibitor N-(9-hydroxy-9-fluorenecarboxy) prolyl trans-4-aminocyclohexylmethyl amide (L-372,460): Coapplication of analogue synthesis on solid support
11. Automated Laboratories
12. Secrets of actin-based motility revealed by a bacterial pathogen
13. Enteropathogenic Escherichia coli (EPEC) attachment to epithelial cells: Exploiting the host cell cytoskeleton from the outside
14. Brefeldin A: The advantage of being uncompetitive
15. Blebbistatin: A cell permeable inhibitor of non-muscle myosin II
16. A small-molecule inhibitor of skeletal muscle myosin II
17. Natural products as a resource for new drugs
18. Effects of cytochalasin and phalloidin on actin
19. Toxoplasma gondii exploits host low-density lipoprotein receptor-mediated endocytosis for cholesterol acquisition
20. Well Read
21. Chemical genetics: Exploring and controlling cellular processes with chemical probes
22. Specificity and mechanism of action of some commonly used protein kinase inhibitors
23. Identification of small-molecule inhibitors of interaction between the BH3 domain and Bcl-xL
24. Towards a truly integrative biology through the functional genomics of yeast
25. Toxoplasma invasion of mammalian cells is powered by the actin cytoskeleton of the parasite
26. Comprehensive survey of combinatorial library synthesis: 2000
27. A genetic screen for mutations affecting embryogenesis in zebrafish
28. Let's get specific: The relationship between specificity and affinity
29. Use of a drug-resistant mutant of stress-activated protein kinase 2a/p38 to validate the in vivo specificity of SB 203580
30. Ergostatin a Golgi disrupting compound targets ARF GTPase
31. Microbial resistance: Novel screens for a contemporary problem
32. Pharmacological rescue of mutant p53 conformation and function
33. General method for rapid synthesis of multicomponent peptide mixtures
34. A comparative study of the actin-based motilities of the pathogenic bacteria Listeria monocytogenes, Shigella flexneri and Rickettsia conorii
35. Bioactive agents from natural sources: Trends in discovery and application
36. Exploiting chemical libraries structure and genomics in the search for kinase inhibitors
37. Identification of a class of small molecule inhibitors of the sirtuin family of NAD-dependent deacetylases by phenotypic screening
38. A Chemistry Set for Life
39. Chlamydia trachomatis interrupts an exocytic pathway to acquire endogenously synthesized sphingomyelin in transit from the Golgi apparatus to the plasma membrane
40. The identification of genes with unique and essential functions in the development of the zebrafish
41. Genetic control of the cell division cycle in yeast
42. Toxoplasma gondii homologue of Plasmodium Apical Membrane Antigen 1 is involved in invasion of host cells
43. Differential interaction with endocytic and exocytic pathways distinguish parasitophorous vacuoles of Coxiella burnetii and Chlamydia trachomatis
44. Dynamics of actin-based movement by Rickettsia rickettsii in vero cells
45. Functional discovery via a compendium of expression profiles
46. Brefeldin A revealing the fundamental principles governing membrane dynamics and protein transport
47. Probing spindle assembly mechanisms with monastrol, a small molecule inhibitor of the mitotic kinesin Eg5
48. Allele-specific activators and inhibitors for kinesin
49. Novel HTS technologies: Development of miniaturized assay formats
50. Combinatorial chemistry and mass spectrometry in the 21st century drug discovery laboratory
51. Intimin-dependent binding of enteropathogenic Escherichia coli to host cells triggers novel signaling events including tyrosine phosphorylation of phospholipase C-gamma 1
52. The application of high-throughput screening to novel lead discovery
53. Characterization and function of the microsporidian polar tube: A review
54. Polar tube proteins of microsporidia of the family encephalitozoonidae
55. Http://C.elegans: Mining the functional genomic landscape
56. Chemistry or biology: Which comes first after the genome is sequenced?
57. Recent developments in structure-based drug design
58. Intracellular targets of cyclin-dependent kinase inhibitors: Identification by affinity chromatography using immobilized inhibitors
59. Intracellular targets of paullones: Identification following affinity purification on immobilized inhibitor
60. A plastid of probable green algal origin in Apicomplexan parasites
61. The 'One-Bead-One-Compound' combinatorial library method
62. Printing proteins as microarrays for high-throughput function determination
63. Diverse viewpoints on computational aspects of molecular diversity
64. Drug target validation and identification of secondary drug target effects using DNA microarrays
65. Assay technologies for screening ion channel targets
66. Small molecule inhibitor of mitotic spindle bipolarity identified in a phenotype-based screen
67. Small Molecule Approach to Dissect Centrosome Duplication in Mammalian Cells
68. Dissecting glycoprotein biosynthesis by the use of specific inhibitors
69. Apicomplexan plastids as drug targets
70. Eponemycin exerts its antitumor effect through the inhibition of proteasome function
71. Towards a Pharmacological Genetics
72. Cytoskeleton of apicomplexan parasites
73. Simple selection criteria for drug-like chemical matter
74. Mutations affecting segment number and polarity in Drosophila
75. Application of physiologically active substances isolated from natural resources to pharmacological studies
76. Complex synthetic chemical libraries indexed with molecular tags
77. Direct proof that the primary site of action of cytochalasin on cell motility processes is actin
78. Use of biomimetic diversity-oriented synthesis to discover galanthamine-like molecules with biological properties beyond those of the natural product
79. Local actin polymerization and dynamin recruitment in SV40-induced internalization of caveolae
80. Small molecule developmental screens reveal the logic and timing of vertebrate development
81. A chemical inhibitor of N-WASP reveals a new mechanism for targeting protein interactions
82. Brefeldin A acts to stabilize an abortive ARF-GDP-Sec7 domain protein complex: Involvement of specific residues of Sec7 domain
83. The microsporidian spore invasion tube. IV. Discharge activation begins with pH-triggered Ca2+ influx
84. Combinatorial diffusion assay used to identify topically active melanocyte-stimulating hormone receptor antagonists
85. TgM2AP participates in Toxoplasma gondii invasion of host cells and is tightly associated with the adhesive protein TgMIC2
86. Techview: Drug discovery. Integrating combinatorial synthesis and bioassays
87. Toxoplasmosis
88. A trypanosome-soluble factor induces IP3 formation intracellular Ca2+ mobilization and microfilament rearrangement in host cells
89. A cyclin-dependent kinase inhibitor inducing cancer cell differentiation: Biochemical identification using Xenopus egg extracts
90. Myoseverin, a microtubule-binding molecule with novel cellular effects
91. The successful partnership of biotechnology based screen development with high throughput screening
92. Effect of cytochalasin D on Toxoplasma gondii cell entry
93. Chemical genetics resulting from a passion for synthetic organic chemistry
94. The mechanism of action of cyclosporinA and FK506
95. Immunofluorescent localization of myosin at the anterior pole of the coccidian Toxoplasma gondii
96. Target-based drug discovery for the development of novel antiinfectives
97. Modern Drug Design and Discovery: An Overview
98. Engineering unnatural nucleotide specificity for Rous sarcoma virus tyrosine kinase to uniquely label its direct substrates
99. Acquisition of a Specific and Potent PTP1B Inhibitor from a Novel Combinatorial Library and Screening Procedure
100. Aggregation of membrane-associated actin filaments following localized adherence of enteropathogenic Escherichia coli to HeLa cells
101. The anti-angiogenic agent fumagillin covalently binds and inhibits the methionine aminopeptidase, MetAP-2
102. Toxoplasma gondii: Kinetics of stage-specific protein expression during tachyzoitebradyzoite conversion in vitro
103. Chemical genetics: Ligand-based discovery of gene function
104. High-throughput screening of small molecules in miniaturized mammalian cell-based assays involving post-translational modifications
105. An alkylsilyl-tethered, high-capacity solid support amenable to diversity-oriented synthesis for-one-bead one-stock solution chemical genetics
106. Ligand discovery using encoded combinatorial libraries
107. Isolation of cDNAs encoding cellular drug-binding proteins using a novel expression cloning procedure: Drug-western
108. The late chlamyclial inclusion membrane is not derived from the endocytic pathway and is relatively deficient in host proteins
109. A small nonpeptidyl mimic of granulocyte-colony-stimulating factor
110. Actin filaments and the growth, movement, and spread of the intracellular bacterial parasite, Listeria monocytogenes
111. New technologies for chemical genetics
112. 96-well plates providing high optical resolution for high-throughput immunofluorescence-based screening of monoclonal antibodies against Toxoplasma gondii
113. An information-intensive approach to the molecular pharmacology of cancer
114. Target-based drug discovery for malaria, leishmaniasis and trypanosomiasis
115. 4-Phenylthiazole derivatives inhibit IL-6 secretion in osteoblastic cells and suppress bone weight loss in ovariectornized mice
116. A small molecule approach to cell motility
117. Discovery of a small molecule insulin mimetic with antidiabetic activity in mice
118. Global analysis of protein activities using proteome chips