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Volumn 281, Issue 5374, 1998, Pages 257-259

A small, nonpeptidyl mimic of granulocyte-colony-stimulating factor

Author keywords

[No Author keywords available]

Indexed keywords

GRANULOCYTE COLONY STIMULATING FACTOR;

EID: 0032504007     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.281.5374.257     Document Type: Article
Times cited : (162)

References (28)
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    • note
    • IL-3 was withdrawn from NFS60 cells. A cytokine-independent variant of NFS60 grew out, which was used for transfection experiments. The G-CSF signaling characteristics of this line are similar to the parental NFS60S. This line was transfected by electroporation with the plasmid 4×TTCC1Ctkluc, which contains four copies of the STAT-binding element 5′-GATCTGCTTCCCGGAACGT-3′ cloned into the plasmid tk-luc (21), and the plasmid pSV2neo, which confers neomycin resistance. Single drug-resistant clones were isolated by limiting dilution and screened for increased luciferase expression after a 2.5-hour exposure to 10 ng/ml G-CSF.
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    • note
    • The plasmid RSVluc, containing the Rous sarcoma virus long terminal repeat linked to luciferase, was transfected into the cytokine-independent NFS60 line with the plasmid pSV2neo conferring neomycin resistance. Single drug-resistant clones were isolated by limiting dilution and were screened for luciferase production before and after a 2.5-hour treatment with 10 ng/ml G-CSF. A clone showing no response to G-CSF was expanded.
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    • note
    • We thank C. Sincich and P. Sanders for technical assistance and R. Chedester for help with the manuscript.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.