Intimin-dependent binding of enteropathogenic Escherichia coli to host cells triggers novel signaling events, including tyrosine phosphorylation of phospholipase C-γ1

Infection and Immunity

Volumn 65, Issue 7, 1997, Pages 2528-2536

  Kenny, Brendan ^a   Brett Finlay, B ^a,b  

^a UNIVERSITY OF BRITISH COLUMBIA   (Canada)

^b UNIVERSITY OF BRITISH COLUMBIA   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

CYTOCHALASIN D; ESCHERICHIA COLI ENTEROTOXIN; PHOSPHOLIPASE C;

ACTIN POLYMERIZATION; ANTIGEN BINDING; ARTICLE; DEPHOSPHORYLATION; DRUG RECEPTOR BINDING; ENZYME INHIBITION; EPITHELIUM CELL; ESCHERICHIA COLI; HOST CELL; HUMAN; HUMAN CELL; IMMUNE RESPONSE; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN PHOSPHORYLATION; PROTEIN PROTEIN INTERACTION;

EID: 1842376874     PISSN: 00199567     EISSN: None     Source Type: Journal    
DOI: 10.1128/iai.65.7.2528-2536.1997     Document Type: Article

References (30)

1. Baldwin, T. J., W. Ward, A. Aitken, S. Knutton, and P. H. Williams. 1991. Elevation of intracelluar free calcium levels in HEp-2 cells infected wild enteropathogenic Escherichia coli. Infect. Immun. 59:1544-1604.
2. Berridge, M. J. 1943. Inositol triphosphate and calcium signaling. Nature 361:315-325.
3. Bliska, J. B., K. Guan, J. E. Dixon, and S. Falkow. 1991. Tyrosine phosphate hydrolysis of host proteins by an essential Yersinia virulence determinant. Proc. Natl. Acad. Sci. USA 88:1187-1191.
4. Donnenbery, M. S., S. B. Calderwood, A. Donohue-Rolfe, G. T. Keusch, and J. B. Kaper. 1990. Construction and analysis of TnphoA mutants of enteropathogenic Escherichia coli unable to invade HEp-2 cells. Infect. Immun. 58:1565-1571.
5. Donnenberg, M. S., and J. B. Kaper. 1991. Construction of an eae deletion mutant of enteropathogenic Escherihia coli by using a positive-selection suicide vector. Infect. Immun. 59:43111-4317.
6. Donnenberg, M. S., J. A. Giron, J. P. Nataro, and J. B. Kaper. 1992. A plasmid-encoded type IV fimbrial gene of enteropathogenic Escherichia coli associated with localized adherence. Mol. Microbiol. 6:3427-3437.
7. Donnenberg, M. S., J. Yu, and J. B. Kuper. 1993. A second chromosomal gene necessary for intimate attachment of enteropathogenic Escherichia coli to epithelial cells. J. Bacteriol. 175:4670-4680.
8. Dytoc, M., L. Fedorko, and P. M. Sherman. 1994. Signal transduction in human epithelial cells infected with attaching and effacing Escherichia coli in vitro. Gastroenterology 106:1150-11161.
9. Finlay, B. B., I. Rosenshine, M. S. Donnenberg, and J. B. Kaper. 1992. Cytoskeletal composition of attaching and effacing lesions associated with enteropathogenic Escherichia coli adherence to HeLa cells. Infect. Immun. 60:2541-2543.
10. Foubister, V., I. Rosenshine, and B. B. Finlay. 1994. A diarrheal pathogen, enteropathogenic Escherichia coli (EPEC), triggers a flux of inositol phosphates in infected epithelial cells. J. Exp. Med. 179:993-998.
11. Foubister, V., I. Rosenshine, M. S. Donnenberg, and B. B. Finlay. 1994. The eaeB gene of enteropathogenic Escherichia coli is necessary for signal transduction in epithelial cells. Infect. Immun. 62:3038-3040.
12. Goldschidt-Clermont, P.J., J. K. Kim, S. G. R. Machesky, and T. D. Pollard. 1991. Regulation of phospholipase C-γ1 by profilin and tyrosine phosphorylation. Science 251:1231-1233.
13. Gomez-Duarte, O. G., and J. B. Kaper. 1995. A plasmid-encoded regulatory region activates chromosomal eaeA expression in enteropathogenic Escherichia coli. Infect. Immun. 631767-1776.
14. Jarvis, K. G., J. A. Giron, A. E. Jerse, T. K. McDaniel, M. S. Donnenberg, and J. B. Kaper. 1995. Enteropathogenic Escherichia coli contains a specialised secretion system necessary for the export of proteins involved in attaching and effacing lesion formation. Proc. Natl. Acad. Sci. USA 92:7996-8000.
15. Jerse, A. E., J. Yu, B. D. Tall, and J. B. Kaper. 1990. A genetic locus of enteropathogenic E. coli necessary for the production of attaching and effacing lesions on tissue culture cells. Proc. Natl. Acad. Sci. USA 87:7839-7843.
16. Kenny, B., and B. B. Finlay. 1995. Secretion of proteins by enteropathogenic E. coli which mediate signaling in host epithelial cells. Proc. Natl. Acad. Sci. USA 97:7991-7995.
17. Kenny, B., L.-C. Lui, B. B. Finlay, and M. S. Donnenberg. 1996. EspA, a protein secreted by enteropathogenic Eschericha coli (EPEC), is required to induce signals in epithelial cells. Mol. Microbiol. 20:313-323.
18. Knutton, S., D. R. Lloyd, and A. S. McNeish. 1987. Adhesion of enteropathogenic Escherichia coli to human intestinal enterocytes and cultured intestinal mucosa. Infect. Immun. 55:64-77.
19. Knutton, S., I. Baldwin, P. H. Williams, and A. S. McNeish. 1989. Actin accumulation at sites of bacterial adhesion to tissue culture cells: basis of a new diagnostic test for enteropathogenic and enterohemorrhagic Esherichia coli. Infect. Immun. 57:1240-1298.
20. Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of ihc head of bacteriophage T4. Nature 227:680-685.
21. Manjarrez-Hernandez, H. A.,T. J. Baldwin, A. Aitken, S. Knutton, and P. H. Williams. 1992. Intestinal epithelial cell protein phosphorylation in enteropathogenic Escherichia coli diarrhoae. Lancet 339:521-523.
22. Moon, H. W., S. C. Whipp, R. A. Argenzio, M. M. Levine, and R. A. Giannella. 1483. Attaching and effacing activities of rabbit and human enteropathogenic Escherichia coli in pig and rabbit intestines. Infect. Immun. 41:1340-1351.
23. Rijnboutt, S., H. M. Aerts, H. J. Geuze, J. M. Tayer, and G. J. Strous. 1991. Mannose 6-phosphate independent membrane association of cathepsin D, glucocerebrosidase, and sphingolipid-activated protein in HepG2 cells. J. Biol. Chem. 266:4862-4868.
24. Robins-Browne, R. M. 1987. Traditional enteropathogenic Esherichia coli of infantile diarrhea. Rev. Infect. Dis. 9:28-53.
25. Rosenshine, I., M. S. Donnenberg, J. B. Kaper, and B. B. Finlay. 1992. Signal exchange between enteropathogenic Escherichia coli (EPEC) and epithelial cells: EPEC induce tyrosine phosphorylation of host cell protein to initiate cytoskeletal rearrangement and bacterial uptake. EMBO J. 11:3551-3560.
26. Rusenshine, I., S. Ruschkowski, und B. B. Finlay. 1996. Expression of at- taching/effacing activity by enteropathogenic Escherichia colt depends on growth phase, temperature, and protein synthesis upon contact with epithelial cells. Infect. Immun. 64:966-973.
27. Rosenshine, I., S. Ruschkowski, M. Stein, D. J. Reinscheid, S. D. Mills, and B. B. Finlay. 1996. A pathogenic bacterium triggers epithelial signals to form a functional bacterial receptor that mediates actin psuedopod formation. EMBO J. 15:2613-2624.
28. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual. 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
29. 2. Mol. Cell. Biol. 11:2018-2025.
30. Vuopio-Varkila, J., and G. K. Schuolnik. 1991. Localised adherence by enteropathogenic Escherichia coli is an inducible phenotype associated with the expression of new outer membrane proteins. J. Exp. Med. 174:1167-1177