Relationship between mediterranean dietary polyphenol intake and obesity

Nutrients

Volumn 10, Issue 10, 2018, Pages

  Castro Barquero, Sara ^a,b   Lamuela Raventós, Rosa M ^b,c   Doménech, Mónica ^a,b,c   Estruch, Ramon ^a,b,c  

^a INSTITUT D INVESTIGACIONS BIOMÈDIQUES AUGUST PI I SUNYER IDIBAPS   (Spain)

^b UNIVERSITY OF BARCELONA   (Spain)

^c Instituto de Salud Carlos III   (Spain)

Author keywords

BMI; Catechins; Dietary intake; Olive oil; Resveratrol; Wine

Indexed keywords

ADIPONECTIN; BIOLOGICAL MARKER; C REACTIVE PROTEIN; EXTRA VIRGIN OLIVE OIL; FLAVONOID; HIGH DENSITY LIPOPROTEIN; LOW DENSITY LIPOPROTEIN; PEROXISOME PROLIFERATOR ACTIVATED RECEPTOR ALPHA; PEROXISOME PROLIFERATOR ACTIVATED RECEPTOR DELTA; PEROXISOME PROLIFERATOR ACTIVATED RECEPTOR GAMMA; POLYPHENOL; PREBIOTIC AGENT;

ADIPOCYTE; ADIPOSE TISSUE; BODY COMPOSITION; BODY MASS; BODY WEIGHT LOSS; CARDIOVASCULAR RISK; DIETARY INTAKE; DIETARY PATTERN; DIETARY SUPPLEMENT; DRUG BIOAVAILABILITY; DYSBIOSIS; ESTERIFICATION; FATTY ACID OXIDATION; FOOD FREQUENCY QUESTIONNAIRE; FOOD INTAKE; GLYCEMIC CONTROL; HUMAN; INTESTINE FLORA; LEGUME; LIFESTYLE MODIFICATION; LIPOLYSIS; MEDITERRANEAN DIET; OBESITY; OXIDATIVE STRESS; RED WINE; REVIEW; VEGETABLE; WAIST CIRCUMFERENCE; DRUG EFFECT; FEEDING BEHAVIOR;

ADIPOSE TISSUE; ADIPOSITY; DIET, MEDITERRANEAN; FEEDING BEHAVIOR; GASTROINTESTINAL MICROBIOME; HUMANS; OBESITY; POLYPHENOLS; WEIGHT LOSS;

EID: 85055073744     PISSN: None     EISSN: 20726643     Source Type: Journal    
DOI: 10.3390/nu10101523     Document Type: Review

References (65)

1. Ng, M.; Fleming, T.; Robinson, M.; Thomson, B.; Graetz, N.; Margono, C.; Mullany, E.C.; Biryukov, S.; Abbafati, C.; Abera, S.F.; et al. Global, regional, and national prevalence of overweight and obesity in children and adults during 1980–2013: A systematic analysis for the Global Burden of Disease Study 2013. Lancet 2014, 384, 766–781. [CrossRef]
2. Williams, E.P.; Mesidor, M.; Winters, K.; Dubbert, P.M.; Wyatt, S.B. Overweight and obesity: Prevalence, Consequences and Causes of a Growing Public Health Problem. Curr. Obes. Rep. 2015, 4, 363–370. [CrossRef] [PubMed]
3. Siriwardhana, N.; Kalupahana, N.S.; Cekanova, M.; Lemieux, M.; Greer, B.; Moustaid-Moussa, N. Modulation of adipose tissue inflammation by bioactive food compounds. J. Nutr. Biochem. 2013, 24, 613–623. [CrossRef] [PubMed]
4. Kalupahana, N.S.; Moustaid-Moussa, N.; Claycombe, K.J. Immunity as a link between obesity and insulin resistance. Mol. Asp. Med. 2012, 33, 26–34. [CrossRef] [PubMed]
5. Esposito, K.; Kastorini, C.M.; Panagiotakos, D.B.; Giugliano, D. Mediterranean diet and weight loss: Meta-analysis of randomized controlled trials. Metab. Syndr. Relat. Disord. 2011, 9, 1–12. [CrossRef] [PubMed]
6. Mena, M.P.; Sacanella, E.; Vazquez-Agell, M.; Morales, M.; Fitó, M.; Escoda, R.; Serrano-Martínez, M.; Salas-Salvadó, J.; Benages, N.; Casas, R.; et al. Inhibition of circulating immune cell activation: A molecular antiinflamatory effect of the Mediterranean diet. Am. J. Clin. Nutr. 2009, 89, 248–256. [CrossRef] [PubMed]
7. Urpi-Sarda, M.; Casas, R.; Chiva-Blanch, G.; Romero-Mamamni, E.S.; Valderas-Martínez, P.; Arranz, S.; Andres-Lacueva, C.; Llorach, R.; Medina-Remón, A.; Lamuela-Raventos, R.M.; et al. Virgin olive oil and nuts as key foods of the Mediterranean diet effects on inflammatory biomarkers related to atherosclerosis. Pharmacol. Res. 2012, 65, 577–583. [CrossRef] [PubMed]
8. Marin, C.; Ramirez, R.; Delgado-Lista, J.; Yubero-Serrano, E.M.; Perez-Martinez, P.; Carracedo, J.; Garcia-Rios, A.; Rodriguez, F.; Gutierrez-Mariscal, F.M.; Gomez, P.; et al. Mediterranean diet reduces endothelial damage and improves the regenerative capacity of endothelium. Am. J. Clin. Nutr. 2011, 93, 267–274. [CrossRef] [PubMed]
9. Dai, J.; Jones, D.P.; Goldberg, J.; Ziegler, T.R.; Bostick, R.M.; Wilson, P.W.; Manatunga, A.K.; Shallenberger, L.; Jones, L.; Vaccarino, V. Association between adherence to the Mediterranean diet and oxidative stress. Am. J. Clin. Nutr. 2011, 88, 1364–1370.
10. Jones, J.L.; Fernandez, M.L.; McIntosh, M.S.; Najm, W.; Calle, M.C.; Kalynych, C.; Vukich, C.; Barona, J.; Ackermann, D.; Kim, J.E.; et al. A Mediterranean-style low-glycemic-load diet improves variables of metabolic syndrome in women, in addition of a phytochemical-rich medical food enhances benefits on lipoprotein metabolism. J. Clin. Lipidol. 2011, 5, 188–196. [CrossRef] [PubMed]
11. Hernáez, Á.; Castañer, O.; Elosua, R.; Pintó, X.; Estruch, R.; Salas-Salvadó, J.; Corella, D.; Arós, F.; Serra-Majem, L.; Fiol, M.; et al. Mediterranean Diet Improves High-Density Lipoprotein Function in High-Cardiovascular-Risk Individuals. Circulation 2017, 135, 633–643. [CrossRef] [PubMed]
12. Fitó, M.; Cladellas, M.; de la Torre, R.; Martí, J.; Muñoz, D.; Schröder, H.; Alcántara, M.; Pujadas-Bastardes, M.; Marrugat, J.; López-Sabater, M.C.; et al. Anti-inflammatory effect of virgin olive oil in stable coronary disease patients: A randomized, crossover, controlled trial. Eur. J. Clin. Nutr. 2008, 62, 570–574. [CrossRef] [PubMed]
13. Phenol-Explorer, An Online Comprehensive Database on Polyphenol Contents in Foods. 2010. Available online: http://www.phenol-explorer.eu (accessed on 21 September 2018).
14. Tresserra-Rimbau, A.; Rimm, E.B.; Medina-Remon, A.; Martinez-Gonzalez, M.A.; dela Torre, R.; Corella, D.; Salas-Salvador, J.; Gómez-Garcia, E.; Lapetra, J.; Arós, F.; et al. Inverse association between habitual polyphenol intake and incidence of cardiovascular events in the PREDIMED study. Nutr. Metab. Cardiovasc. Dis. 2014, 24, 639–647. [CrossRef] [PubMed]
15. Mancini, J.G.; Filion, K.B.; Atallah, R.; Eisenberg, M.J. Systematic Review of the Mediterranean Diet for Long-Term Weight Loss. Am. J. Med. 2016, 129, 407–415. [CrossRef] [PubMed]
16. Huo, R.; Du, T.; Xu, Y.; Xu, W.; Chen, X.; Sun, K.; Yu, X. Effects of Mediterranean-style diet on glycemic control, weight loss and cardiovascular risk factors among type 2 diabetes individuals: A meta-analysis. Eur. J. Clin. Nutr. 2015, 69, 1200–1208. [CrossRef] [PubMed]
17. Probst, Y.; Guan, V.; Kent, K. A systematic review of food composition tools used for determining dietary polyphenol intake in estimated intake studies. Food Chem. 2018, 238, 146–152. [CrossRef] [PubMed]
18. Zamora-Ros, R.; Knaze, V.; Rothwell, J.A.; Hémon, B.; Moskal, A.; Overvad, K.; Tjønneland, A.; Kyrø, C.; Fagherazzi, G.; Boutron-Ruault, M.C.; et al. Dietary polyphenol intake in Europe: The European Prospective Investigation into Cancer and Nutrition (EPIC) study. Eur. J. Nutr. 2016, 55, 1359–1375. [CrossRef] [PubMed]
19. Pérez-Jiménez, J.; Fezeu, L.; Touvier, M.; Arnault, N.; Manach, C.; Hercberg, S.; Galan, P.; Scalbert, A. Dietary intake of 337 polyphenols in French Adults. Am. J. Clin. Nutr. 2011, 93, 1220–1228. [CrossRef] [PubMed]
20. Sofi, F.; Dinu, M.; Pagliai, G.; Marcucci, R.; Casini, A. Validation of a literatura-based adherence score to Mediterranean diet: The MEDI-LITE score. Int. J. Food Sci. Nutr. 2017, 68, 757–762. [CrossRef] [PubMed]
21. Godos, J.; Marventano, S.; Mistretta, A.; Galvano, F.; Grosso, G. Dietary sources of polyphenols om the Mediterranean healthy Eating, Aging and Lifestyle (MEAL) study cohort. Int. J. Food. Sci. Nutr. 2017, 68, 750–756. [CrossRef] [PubMed]
22. Godos, J.; Rapisarda, G.; Marventano, S.; Galvano, F.; Mistretta, A.; Grosso, G. Association between polyphenol intake and adherence to the Mediterranean diet in Sicily, soythern Italy. NFS J. 2017, 8, 1–7. [CrossRef]
23. Tresserra-Rimbau, A.; Medina-Remón, A.; Pérez-Jiménez, J.; Martínez-González, M.A.; Covas, M.I.; Corella, D.; Salas-Salvador, J.; Gómez-Gracia, E.; Lapetra, J.; Arós, F.; et al. Dietary intake and major food sources of polyphenols in a Spanish population at high cardiovascular risk: The PREDIMED study. Nutr. Metab. Cardiovasc. Dis. 2013, 23, 953–959. [CrossRef] [PubMed]
24. Covas, M.I.; Nyyssonen, K.; Poulsen, H.E.; Kaikkonen, J.; Zunft, H.J.; Kiesewetter, H.; Gaddi, A.; de la Torre, R.; Mursu, J.; Bäumler, H.; et al. The effect of polyphenols in olive oil on heart disease risk factors: A randomized trial. Ann. Intern. Med. 2006, 145, 333–341. [CrossRef] [PubMed]
25. EFSA Panel on Dietetic Products NaAN. Scientific Opinion on the substantiation of health claims related to polyphenols in olive and protection of LDL particles from oxidative damage (ID 1333, 1638, 1639, 1696, 2865), maintenance of normal blood HDLcholesterol concentrations (ID 1639), maintenance of normal blood pressure (ID 3781), “anti-inflammatory properties” (ID 1882), “contributes to the upper respiratory tract health” (ID 3468), “can help to maintain a normal function of gastrointestinal tract” (3779), and “contributes to body defences against external agents” (ID 3467) pursuant to Article 13(1) of Regulation (EC) No 1924/20061. EFSA J. 2011, 9, 2033.
26. Barth, S.W.; Koch, T.C.L.; Watzl, B.; Dietrich, H.; Will, F.; Bub, A. Moderate effects of apple juice consumption on obesity-related markers in obese men: Impact of diet-gene interaction on body fat content. Eur. J. Nutr. 2012, 51, 841–850. [CrossRef] [PubMed]
27. Cases, J.; Romain, C.; Dallas, C.; Gerbi, A.; Cloarec, M. Regular consumption of Fiit-ns, a polyphenol extract from fruit and vegetables frequently consumed within the Mediterranean diet, improves metabolic ageing of obese volunteers: A. randomized, double-blind, parallel trial. Int. J. Food. Sci. Nutr. 2015, 66, 120–125. [CrossRef] [PubMed]
28. Guo, X.; Tresserra-Rimbau, A.; Estruch, R.; Martinez-Gonzalez, M.A.; Medina-Remon, A.; Fitó, M.; Corella, D.; Salas-Salvadó, J.; Portillo, M.P.; Moreno, J.J.; et al. Polyphenol levels are inversely correlated with body weight and obesity in an elderly population after 5 years of follow up (The Randomised PREDIMED Study). Nutrients 2017, 9, 452. [CrossRef] [PubMed]
29. Marranzano, M.; Ray, S.; Godos, J.; Galvano, F. Association between dietary flavonoids intake and obesity in a cohort of adults living in the Mediterranean area. Int. J. Food Sci. Nutr. 2018, 26, 1–10. [CrossRef] [PubMed]
30. Wang, S.; Moustaid-Moussa, N.; Chen, L.; Mo, H.; Shastri, A.; Su, R.; Bapat, P.; Kwun, I.; Shen, C.L. Novel insights of dietary polyphenols and obesity. J. Nutr. Biochem. 2014, 25, 1–18. [CrossRef] [PubMed]
31. Hursel, R.; Viechtbauer, W.; Westerterp-Plantenga, M.S. The effects of green tea on weight loss and weight maintenance: A meta-analysis. Int. J. Obes. 2009, 33, 956–961. [CrossRef] [PubMed]
32. Jurgens, T.M.; Whelan, A.M.; Killian, L.; Doucette, S.; Kirk, S.; Foy, E. Green tea for weight loss and weight maintenance in overweight or obese adults. Cochrane Database Syst. Rev. 2012, 12, CD008650. [CrossRef] [PubMed]
33. Kim, S.; Jin, Y.; Choi, Y.; Park, T. Resveratrol exerts anti-obesity effects via mechanisms involving down-regulation of adipogenic and inflammatory processes in mice. Biochem. Pharmacol. 2011, 81, 1343–1351. [CrossRef] [PubMed]
34. Tome-Carneiro, J.; Gonzalvez, M.; Larrosa, M.; Garcia-Almagro, F.J.; Aviles-Plaza, F.; Parra, S.; Yáñez-Gascón, M.J.; Ruiz-Ros, J.A.; García-Conesa, M.T.; Tomás-Barberán, F.A.; et al. Consumption of a grape extract supplement containing resveratrol decreases oxidized LDL and ApoB in patients undergoing primary prevention of cardiovascular disease: A triple-blind, 6-month follow-up, placebo-controlled, randomized trial. Mol. Nutr. Food Res. 2013, 56, 810–821. [CrossRef] [PubMed]
35. Meydani, M.; Hasan, S.T. Dietary polyphenols and obesity. Nutrients 2010, 2, 737–751. [CrossRef] [PubMed]
36. Ramirez-Bosca, A.; Soler, A.; Carrion, M.A.; Diaz-Alperi, J.; Bernd, A.; Quintanilla, C.; Quintanilla Almagro, E.; Miguel, J. An hydroalcoholic extract of curcuma longa lowers the apo B./apo A. ratio. Implications for atherogenesis prevention. Mech. Ageing Dev. 2000, 119, 41–47. [CrossRef]
37. Cox, L.M.; Blaser, M.J. Pathways in microbe-induced obesity. Cell Met. 2013, 17, 883–894. [CrossRef] [PubMed]
38. Rupasinghe, H.P.V.; Sekhon-Loodu, S.; Mantso, T.; Panayiotidis, M.I. Phytochemicals in regulating fatty acids β-oxidation: Potential underlying mechanisms and their involvement in obesity and weight loss. Pharmacol. Ther. 2016, 165, 153–163. [CrossRef] [PubMed]
39. Tenore, G.C.; Stiuso, P.; Campiglia, P.; Novellino, E. In vitro hypoglycaemic and hypolipidemic potential of white tea polyphenols. Food Chem. 2013, 141, 2379–2384. [CrossRef] [PubMed]
40. Cummings, J.H.; Macfarlane, G.T. The control and consequences of bacterial fermentation in the human colon. J. Appl. Bacteriol. 1991, 70, 443–459. [CrossRef] [PubMed]
41. Hester, C.M.; Jala, V.R.; Langille, M.G.; Umar, S.; Greiner, K.A.; Haribabu, B. Fecal microbes, short chain fatty acids, and colorectal cancer across racial/ethnic groups. World J. Gastroenterol. 2015, 21, 2759–2769. [CrossRef] [PubMed]
42. Fernandes, J.; Su, W.; Rahat-Rozenbloom, S.; Wolever, T.M.; Comelli, E.M. Adiposity, gut microbiota and faecal short chain fatty acids are linked in adult humans. Nutr. Diabetes 2014, 4, e121. [CrossRef] [PubMed]
43. Den Besten, G.; Bleeker, A.; Gerding, A.; van Eunen, K.; Having, R.; van Dijk, T.H.; Oosterveer, M.H.; Jonker, J.W.; Groen, A.K.; Reijngoud, D.J.; et al. Short-chain fatty acids protect against high-fat diet-induced obesity via a PPARgamma-dependent switch from lipogenesis to fat oxidation. Diabetes 2015, 64, 2398–2408. [CrossRef] [PubMed]
44. De Vadder, F.; Kovatcheva-Datchary, P.; Goncalves, D.; Vinera, J.; Zitoun, C.; Duchampt, A.; Bäckhed, F.; Mithieux, G. Microbiota-generated metabolites promote metabolic benefits via gut-brain neural circuits. Cell 2014, 156, 84–96. [CrossRef] [PubMed]
45. Lin, H.V.; Frassetto, A.; Kowalik, E.J., Jr.; Nawrocki, AR.; Lu, M.M.; Kosinski, J.R.; Hubert, J.A.; Szeto, D.; Yao, X.; Forrest, G.; et al. Butyrate and propionate protect against diet-induced obesity and regulate gut hormones via free fatty acid receptor 3-independent mechanisms. PLoS ONE. 2012, 7, e35240. [CrossRef] [PubMed]
46. Shah, S.H.; Crosslin, D.R.; Haynes, C.S.; Nelson, S.; Turer, C.B.; Stevens, R.D.; Muehlbauer, M.J.; Wenner, B.R.; Bain, J.R.; Laferrère, B.; et al. Branched-chain amino acid levels are associated with improvement in insulin resistance with weight loss. Diabetologia 2012, 55, 321–330. [CrossRef] [PubMed]
47. Ridaura, V.K.; Faith, J.J.; Rey, F.E.; Cheng, J.; Duncan, A.E.; Kau, A.L.; Griffin, N.W.; Lombard, V.; Henrissat, B.; Bain, J.R.; et al. Gut microbiota from twins discordant for obesity modulate metabolism in mice. Science 2013, 341, 1241214. [CrossRef] [PubMed]
48. Cardona, F.; Andrés-Lacueva, C.; Tulipani, S.; Tinahones, F.Q.; Queipo-Ortuño, M.I. Benefits of polyphenols on gut microbiota and implications in human health. J. Nutr. Biochem. 2013, 24, 1415–1422. [CrossRef] [PubMed]
49. Duda-Chodak, A.; Tarko, T.; Satora, P.; Sroka, P. Interaction of dietary compounds, especially polyphenols, with the intestinal microbiota: A review. Eur. J. Nutr. 2015, 54, 325–341. [CrossRef] [PubMed]
50. Noratto, G.D.; Garcia-Mazcorro, J.F.; Markel, M.; Martino, H.S.; Minamoto, Y.; Steiner, J.M.; Byrne, D.; Suchodolski, J.S.; Mertens-Talcott, S.U. Carbohydrate-free peach (Prunus persica) and plum (Prunus domestica) juice affects fecal microbial ecology in an obese animal model. PLoS ONE 2014, 9, e101723. [CrossRef] [PubMed]
51. Anhê, F.F.; Varin, T.V.; Le Barz, M.; Desjardins, Y.; Levy, E.; Roy, D.; Marette, A. Gut microbiota dysbiosis in obesity-linked metabolic diseases and prebiotic potential of polyphenol-rich extracts. Curr. Obes. Rep. 2015, 4, 389–400. [CrossRef] [PubMed]
52. Choy, Y.Y.; Quifer-Rada, P.; Holstege, D.M.; Frese, S.A.; Calvert, C.C.; Mills, D.A.; Lamuela-Raventós, R.M.; Waterhouse, A.L. Phenolic metabolites and substantial microbiome changes in pig feces by ingesting grape seed proanthocyanidins. Food Funct. 2014, 5, 2298–2308. [CrossRef] [PubMed]
53. Quifer-Rada, P.; Choy, Y.Y.; Calvert, C.C.; Waterhouse, A.L.; Lamuela-Raventós, R.M. Use of metabolomics and lipidomics to evaluate the hypocholestreolemic efffect of Proanthocyanidins from grape seed in a pig model. Mol. Nutr. Food Res. 2016, 60, 1–9. [CrossRef] [PubMed]
54. Lee, M.S.; Kim, C.T.; Kim, Y. Green tea (−)-epigallocatechin-3-gallate reduces body weight with regulation of multiple genes expression in adipose tissue of diet-induced obese mice. Ann. Nutr. Metab. 2009, 54, 151–157. [CrossRef] [PubMed]
55. Chung, J.H.; Manganiello, V.; Dyck, J.R. Resveratrol as a calorie restriction mimetic: Therapeutic implications. Trends Cell Biol. 2012, 22, 546554. [CrossRef] [PubMed]
56. Aguirre, L.; Fernandez-Quintela, A.; Arias, N.; Portillo, M.P. Resveratrol: Anti-obesity mechanisms of action. Molecules 2014, 19, 18632–18655. [CrossRef] [PubMed]
57. Skudelska, K.; Szkudelski, T. Resveratrol, obesity and diabetes. Eur. J. Pharmacol. 2010, 635, 1–8. [CrossRef] [PubMed]
58. Ejaz, A.; Wu, D.; Kwan, P.; Meydani, M. Curcumin inhibits adipogenesis in 3T3-L1 adipocytes and angiogenesis and obesity in C57/BL mice. J. Nutr. 2009, 39, 791–797. [CrossRef] [PubMed]
59. Wu, T.; Tang, Q.; Gao, Z.; Yu, Z.; Song, H.; Zheng, X.; Chen, W. Blueberry and mulberry juice prevent obesity development in C57BL/g mice. PLoS ONE 2013. [CrossRef] [PubMed]
60. Vogel, P.; Machado, I.K.; Garavaglia, J.; Zani, V.T.; De Souza, D.; Morelo Dal Bosco, S. Polyphenols benefits of olive leaf (Olea europaea L.) to human health. Nutr. Hosp. 2015, 31, 1427–1433.
61. Casado-Díaz, A.; Anter, J.; Müller, S.; Winter, P.; Quesada-Gómez, J.M.; Dorado, G. Transcriptomic analyses of the anti-adipogenic effects of oleuropein in human mesenchymal stem cells. Food Funct. 2017, 8, 1254–1270. [CrossRef] [PubMed]
62. Oi-Kano, Y.; Iwasaki, Y.; Toshiyuki, N.; Watanabe, T.; Goto, T.; Kawada, T.; Watanabe, K.; Iwai, K. Oleuropein aglycone enhances UCP1 expression in brown adipose tissue in high-fat-diet-induced obese rats by activating β-adrenergic signaling. J. Nutr. Biochem. 2017, 40, 209–218. [CrossRef] [PubMed]
63. De la Torre-Carbot, K.; Chávez-Servín, J.L.; Jaúregui, O.; Castellote, A.I.; Lamuela-Raventós, R.M.; Fitó, M.; Covas, M.-I.; Muñoz-Aguayo, D.; López-Sabater, M.C. Presence of virgin olive oil phenolic metabolites in human low density lipoprotein fraction: Determination by high-performance liquid chromatography-electrospray ionization tandem mass spectrometry. Anal. Chim. Acta 2007, 583, 402–410. [CrossRef] [PubMed]
64. Peyrol, J.; Riva, C.; Amiot, M.J. Hydroxytyrosol in the Prevention of Metabolic Syndrome and Related Disorders. Nutrients 2017, 9, 306. [CrossRef] [PubMed]
65. Hao, J.; Shen, W.; Yu, G.; Jia, H.; Li, X.; Feng, Z.; Wang, Y.; Weber, P.; Wertz, K.; Sharman, E.; et al. Hydroxytyrosol promotes mitochondrial biogenesis and mitochondrial function in 3T3-L1 adipocytes. J. Nutr. Biochem. 2010, 21, 634–644. [CrossRef] [PubMed]