A highresolution genome-wide CRISPR/Cas9 viability screen reveals structural features and contextual diversity of the human cell-essential proteome

Molecular and Cellular Biology

Volumn 38, Issue 1, 2018, Pages

  Bertomeu, Thierry ^a   Coulombe Huntington, Jasmin ^a   Chatr aryamontri, Andrew ^a   Bourdages, Karine G ^a   Coyaud, Etienne ^b   Raught, Brian ^b   Xia, Yu ^c   Tyers, Mike ^a  

^a INSTITUTE FOR RESEARCH IN IMMUNOLOGY AND CANCER   (Canada)

^b PRINCESS MARGARET HOSPITAL   (Canada)

^c MCGILL UNIVERSITY   (Canada)

Author keywords

Alternative splicing; CRISPR Cas9; Gene essentiality; Genetic screen; Hypothetical gene; Protein complex; Proteome

Indexed keywords

ANAPHASE PROMOTING COMPLEX; DEOXYRIBONUCLEASE I; GUIDE RNA; MALONYL COENZYME A; NUCLEOPORIN; OCTAMER TRANSCRIPTION FACTOR 4; PROTEOME; RNA POLYMERASE II;

AMINO ACID SEQUENCE; ANIMAL CELL; ANIMAL EXPERIMENT; ARTICLE; B CELL LYMPHOMA; BIOGENESIS; CELL DIVISION; CELL GROWTH; CELL LINEAGE; CELL PROLIFERATION; CELL SURVIVAL; CELL VIABILITY; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEAT; CONTROLLED STUDY; CRISPR-CAS9 SYSTEM; DNA REPLICATION; ENERGY METABOLISM; ESSENTIAL GENE; EXON; EXPRESSED SEQUENCE TAG; GENETIC ANALYSIS; GENETIC CONSERVATION; HUMAN; HUMAN CELL; HUMAN GENOME; HUMAN TISSUE; M PHASE CELL CYCLE CHECKPOINT; MITOCHONDRIAL RIBOSOME; MOUSE; MUTATION RATE; NONHUMAN; OPEN READING FRAME; PRIORITY JOURNAL; PROTEIN DEPLETION; PROTEIN FUNCTION; PROTEIN INTERACTION; PROTEIN STRUCTURE; PROTEIN SUBUNIT; READING FRAME; RECOMBINATION REPAIR; RIBOSOME SUBUNIT; STRESS; CRISPR CAS SYSTEM; GENE LIBRARY; GENETICS; GENOME-WIDE ASSOCIATION STUDY; METABOLISM; PROCEDURES; PROTEOMICS; TUMOR CELL LINE;

CELL LINE, TUMOR; CELL SURVIVAL; CRISPR-CAS SYSTEMS; GENE LIBRARY; GENES, ESSENTIAL; GENOME-WIDE ASSOCIATION STUDY; HUMANS; PROTEOME; PROTEOMICS;

EID: 85038421356     PISSN: 02707306     EISSN: 10985549     Source Type: Journal    
DOI: 10.1128/MCB.00302-17     Document Type: Article

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