Super-Resolution Microscopy: Shedding Light on the Cellular Plasma Membrane

Chemical Reviews

Volumn 117, Issue 11, 2017, Pages 7457-7477

  Stone, Matthew B ^a   Shelby, Sarah A ^a   Veatch, Sarah L ^a  

^a UNIVERSITY OF MICHIGAN   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CELL MEMBRANES; MAMMALS;

CELLULAR ENVIRONMENT; CELLULAR PLASMA MEMBRANES; COLLECTIVE PROCESS; FUNDAMENTAL BUILDING BLOCKS; MEMBRANE COMPONENTS; MICROSCOPY TECHNIQUE; SUPER-RESOLUTION MICROSCOPY; TECHNICAL ADVANCEMENT;

OPTICAL RESOLVING POWER;

ANIMAL; CELL MEMBRANE; HUMAN; LIGHT; MICROSCOPY;

ANIMALS; CELL MEMBRANE; HUMANS; LIGHT; MICROSCOPY;

EID: 85017189143     PISSN: 00092665     EISSN: 15206890     Source Type: Journal    
DOI: 10.1021/acs.chemrev.6b00716     Document Type: Review

References (245)

1. Veatch, S. L.; Keller, S. L. Seeing Spots: Complex Phase Behavior in Simple Membranes Biochim. Biophys. Acta, Mol. Cell Res. 2005, 1746 (3) 172-185 10.1016/j.bbamcr.2005.06.010
2. van Meer, G.; Voelker, D. R.; Feigenson, G. W. Membrane Lipids: Where They Are and How They Behave Nat. Rev. Mol. Cell Biol. 2008, 9 (2) 112-124 10.1038/nrm2330
3. Lewis, R. N. A. H.; McElhaney, R. N. Membrane Lipid Phase Transitions and Phase Organization Studied by Fourier Transform Infrared Spectroscopy Biochim. Biophys. Acta, Biomembr. 2013, 1828 (10) 2347-2358 10.1016/j.bbamem.2012.10.018
4. Elson, E. L.; Fried, E.; Dolbow, J. E.; Genin, G. M. Phase Separation in Biological Membranes: Integration of Theory and Experiment Annu. Rev. Biophys. 2010, 39 (1) 207-226 10.1146/annurev.biophys.093008.131238
5. Heberle, F. A.; Feigenson, G. W. Phase Separation in Lipid Membranes Cold Spring Harbor Perspect. Biol. 2011, 3, a004630 10.1101/cshperspect.a004630
6. Caffrey, M. A Comprehensive Review of the Lipid Cubic Phase or in Meso Method for Crystallizing Membrane and Soluble Proteins and Complexes Acta Crystallogr., Sect. F: Struct. Biol. Commun. 2015, 71, 3-18 10.1107/S2053230X14026843
7. Cullis, P. R.; de Kruijff, B. Lipid Polymorphism and the Functional Roles of Lipids in Biological Membranes Biochim. Biophys. Acta, Rev. Biomembr. 1979, 559 (4) 399-420 10.1016/0304-4157(79)90012-1
8. Frolov, V. A.; Shnyrova, A. V.; Zimmerberg, J. Lipid Polymorphisms and Membrane Shape Cold Spring Harbor Perspect. Biol. 2011, 3 (11) a004747 10.1101/cshperspect.a004747
9. Jouhet, J. Importance of the Hexagonal Lipid Phase in Biological Membrane Organization Front. Plant Sci. 2013, 10.3389/fpls.2013.00494
10. Gruner, S. M.; Cullis, P. R.; Hope, M. J.; Tilcock, C. P. S. Lipid Polymorphism:The Molecular Basis of Nonbilayer Phases Annu. Rev. Biophys. Biophys. Chem. 1985, 14 (1) 211-238 10.1146/annurev.bb.14.060185.001235
11. Seddon, J. M. Structure of the Inverted Hexagonal (HII) Phase, and Non-Lamellar Phase Transitions of Lipids Biochim. Biophys. Acta, Rev. Biomembr. 1990, 1031 (1) 1-69 10.1016/0304-4157(90)90002-T
12. Tenchov, B. On the Reversibility of the Phase Transitions in Lipid-Water Systems Chem. Phys. Lipids 1991, 57 (2) 165-177 10.1016/0009-3084(91)90074-L
13. Levental, K. R.; Lorent, J. H.; Lin, X.; Skinkle, A. D.; Surma, M. A.; Stockenbojer, E. A.; Gorfe, A. A.; Levental, I. Polyunsaturated Lipids Regulate Membrane Domain Stability by Tuning Membrane Order Biophys. J. 2016, 110 (8) 1800-1810 10.1016/j.bpj.2016.03.012
14. Cooper, R. A. Influence of Increased Membrane Cholesterol on Membrane Fluidity and Cell Function in Human Red Blood Cells J. Supramol. Struct. 1978, 8 (4) 413-430 10.1002/jss.400080404
15. Yeagle, P. L. Cholesterol and the Cell Membrane Biochim. Biophys. Acta, Rev. Biomembr. 1985, 822 (3) 267-287 10.1016/0304-4157(85)90011-5
16. Köberlin, M. S.; Snijder, B.; Heinz, L. X.; Baumann, C. L.; Fauster, A.; Vladimer, G. I.; Gavin, A.-C.; Superti-Furga, G. A Conserved Circular Network of Coregulated Lipids Modulates Innate Immune Responses Cell 2015, 162 (1) 170-183 10.1016/j.cell.2015.05.051
17. Shevchenko, A.; Simons, K. Lipidomics: Coming to Grips with Lipid Diversity Nat. Rev. Mol. Cell Biol. 2010, 11 (8) 593-598 10.1038/nrm2934
18. Ernst, R.; Ejsing, C. S.; Antonny, B. Homeoviscous Adaptation and the Regulation of Membrane Lipids J. Mol. Biol. 2016, 428, 4776 10.1016/j.jmb.2016.08.013
19. Dodge, J. T.; Mitchell, C.; Hanahan, D. J. The Preparation and Chemical Characteristics of Hemoglobin-Free Ghosts of Human Erythrocytes Arch. Biochem. Biophys. 1963, 100 (1) 119-130 10.1016/0003-9861(63)90042-0
20. Steck, T. L. The Organization of Proteins in the Human Red Blood Cell Membrane. A Review J. Cell Biol. 1974, 62 (1) 1-19 10.1083/jcb.62.1.1
21. Resh, M. D. Trafficking and Signaling by Fatty-Acylated and Prenylated Proteins Nat. Chem. Biol. 2006, 2 (11) 584-590 10.1038/nchembio834
22. Cho, W.; Stahelin, R. V. Membrane-Protein Interactions in Cell Signaling and Membrane Trafficking Annu. Rev. Biophys. Biomol. Struct. 2005, 34 (1) 119-151 10.1146/annurev.biophys.33.110502.133337
23. Lemmon, M. A. Membrane Recognition by Phospholipid-Binding Domains Nat. Rev. Mol. Cell Biol. 2008, 9 (2) 99-111 10.1038/nrm2328
24. Singer, S. J. The Molecular Organization of Membranes Annu. Rev. Biochem. 1974, 43 (1) 805-833 10.1146/annurev.bi.43.070174.004105
25. Südhof, T. C.; Rothman, J. E. Membrane Fusion: Grappling with SNARE and SM Proteins Science 2009, 323 (5913) 474-477 10.1126/science.1161748
26. White, J. M.; Delos, S. E.; Brecher, M.; Schornberg, K. Structures and Mechanisms of Viral Membrane Fusion Proteins Crit. Rev. Biochem. Mol. Biol. 2008, 43 (3) 189-219 10.1080/10409230802058320
27. Aviram, R.; Manella, G.; Kopelman, N.; Neufeld-Cohen, A.; Zwighaft, Z.; Elimelech, M.; Adamovich, Y.; Golik, M.; Wang, C.; Han, X. et al. Lipidomics Analyses Reveal Temporal and Spatial Lipid Organization and Uncover Daily Oscillations in Intracellular Organelles Mol. Cell 2016, 62 (4) 636-648 10.1016/j.molcel.2016.04.002
28. Bezanilla, M.; Gladfelter, A. S.; Kovar, D. R.; Lee, W.-L. Cytoskeletal Dynamics: A View from the Membrane J. Cell Biol. 2015, 209 (3) 329-337 10.1083/jcb.201502062
29. Luna, E. J.; Hitt, A. L. Cytoskeleton - Plasma Membrane Interactions Science 1992, 258 (5084) 955-964 10.1126/science.1439807
30. Sheetz, M. P. Cell Control by Membrane-cytoskeleton Adhesion Nat. Rev. Mol. Cell Biol. 2001, 2 (5) 392-396 10.1038/35073095
31. Sheetz, M. P.; Sable, J. E.; Döbereiner, H.-G. Continuous Membrane-Cytoskeleton Adhesion Requires Continuous Accommodation to Lipid and Cytoskeleton Dynamics Annu. Rev. Biophys. Biomol. Struct. 2006, 35 (1) 417-434 10.1146/annurev.biophys.35.040405.102017
32. Tserkovnyak, Y.; Nelson, D. R. Conditions for Extreme Sensitivity of Protein Diffusion in Membranes to Cell Environments Proc. Natl. Acad. Sci. U. S. A. 2006, 103 (41) 15002-15007 10.1073/pnas.0606992103
33. Saarikangas, J.; Zhao, H.; Lappalainen, P. Regulation of the Actin Cytoskeleton-Plasma Membrane Interplay by Phosphoinositides Physiol. Rev. 2010, 90 (1) 259-289 10.1152/physrev.00036.2009
34. Machta, B. B.; Veatch, S. L.; Sethna, J. P. Critical Casimir Forces in Cellular Membranes Phys. Rev. Lett. 2012, 109 (13) 138101 10.1103/PhysRevLett.109.138101
35. Baumgart, T.; Hunt, G.; Farkas, E. R.; Webb, W. W.; Feigenson, G. W. Fluorescence Probe Partitioning between Lo/Ld Phases in Lipid Membranes Biochim. Biophys. Acta, Biomembr. 2007, 1768 (9) 2182-2194 10.1016/j.bbamem.2007.05.012
36. Simons, K.; Ikonen, E. Functional Rafts in Cell Membranes Nature 1997, 387 (6633) 569-572 10.1038/42408
37. Kenworthy, A. K. Have We Become Overly Reliant on Lipid Rafts? Talking Point on the Involvement of Lipid Rafts in T-Cell Activation EMBO Rep. 2008, 9 (6) 531-535 10.1038/embor.2008.92
38. Munro, S. Lipid Rafts: Elusive or Illusive? Cell 2003, 115 (4) 377-388 10.1016/S0092-8674(03)00882-1
39. Simons, K.; Gerl, M. J. Revitalizing Membrane Rafts: New Tools and Insights Nat. Rev. Mol. Cell Biol. 2010, 11 (10) 688-699 10.1038/nrm2977
40. Albersdörfer, A.; Feder, T.; Sackmann, E. Adhesion-Induced Domain Formation by Interplay of Long-Range Repulsion and Short-Range Attraction Force: A Model Membrane Study Biophys. J. 1997, 73 (1) 245-257 10.1016/S0006-3495(97)78065-2
41. Banjade, S.; Rosen, M. K. Phase Transitions of Multivalent Proteins Can Promote Clustering of Membrane Receptors eLife 2014, 3, e04123 10.7554/eLife.04123
42. Chaudhuri, A.; Bhattacharya, B.; Gowrishankar, K.; Mayor, S.; Rao, M. Spatiotemporal Regulation of Chemical Reactions by Active Cytoskeletal Remodeling Proc. Natl. Acad. Sci. U. S. A. 2011, 108 (36) 14825-14830 10.1073/pnas.1100007108
43. Mueller, V.; Ringemann, C.; Honigmann, A.; Schwarzmann, G.; Medda, R.; Leutenegger, M.; Polyakova, S.; Belov, V. N.; Hell, S. W.; Eggeling, C. STED Nanoscopy Reveals Molecular Details of Cholesterol- and Cytoskeleton-Modulated Lipid Interactions in Living Cells Biophys. J. 2011, 101 (7) 1651-1660 10.1016/j.bpj.2011.09.006
44. Barak, L. S.; Webb, W. W. Fluorescent Low Density Lipoprotein for Observation of Dynamics of Individual Receptor Complexes on Cultured Human Fibroblasts J. Cell Biol. 1981, 90 (3) 595-604 10.1083/jcb.90.3.595
45. Barak, L. S.; Webb, W. W. Diffusion of Low Density Lipoprotein-Receptor Complex on Human Fibroblasts J. Cell Biol. 1982, 95 (3) 846-852 10.1083/jcb.95.3.846
46. De Brabander, M.; Geuens, G.; Nuydens, R.; Moeremans, M.; De Mey, J. Probing Microtubule-Dependent Intracellular Motility with Nanometre Particle Video Ultramicroscopy (Nanovid Ultramicroscopy) Cytobios 1985, 43 (174S) 273-283
47. Gelles, J.; Schnapp, B. J.; Sheetz, M. P. Tracking Kinesin-Driven Movements with Nanometre-Scale Precision Nature 1988, 331 (6155) 450-453 10.1038/331450a0
48. Schnapp, B. J.; Gelles, J.; Sheetz, M. P. Nanometer-Scale Measurements Using Video Light Microscopy Cell Motil. Cytoskeleton 1988, 10 (1-2) 47-53 10.1002/cm.970100109
49. de Brabander, M.; Nuydens, R.; Ishihara, A.; Holifield, B.; Jacobson, K.; Geerts, H. Lateral Diffusion and Retrograde Movements of Individual Cell Surface Components on Single Motile Cells Observed with Nanovid Microscopy J. Cell Biol. 1991, 112 (1) 111-124 10.1083/jcb.112.1.111
50. Sheets, E. D.; Simson, R.; Jacobson, K. New Insights into Membrane Dynamics from the Analysis of Cell Surface Interactions by Physical Methods Curr. Opin. Cell Biol. 1995, 7 (5) 707-714 10.1016/0955-0674(95)80113-8
51. Sako, Y.; Kusumi, A. Compartmentalized Structure of the Plasma Membrane for Receptor Movements as Revealed by a Nanometer-Level Motion Analysis J. Cell Biol. 1994, 125 (6) 1251-1264 10.1083/jcb.125.6.1251
52. Kusumi, A.; Sako, Y.; Yamamoto, M. Confined Lateral Diffusion of Membrane Receptors as Studied by Single Particle Tracking (Nanovid Microscopy). Effects of Calcium-Induced Differentiation in Cultured Epithelial Cells Biophys. J. 1993, 65 (5) 2021-2040 10.1016/S0006-3495(93)81253-0
53. Dickson, R. M.; Cubitt, A. B.; Tsien, R. Y.; Moerner, W. E. On/off Blinking and Switching Behaviour of Single Molecules of Green Fluorescent Protein Nature 1997, 388 (6640) 355-358 10.1038/41048
54. Heim, R.; Cubitt, A. B.; Tsien, R. Y. Improved Green Fluorescence Nature 1995, 373 (6516) 663-664 10.1038/373663b0
55. Iwane, A. H.; Funatsu, T.; Harada, Y.; Tokunaga, M.; Ohara, O.; Morimoto, S.; Yanagida, T. Single Molecular Assay of Individual ATP Turnover by a Myosin-GFP Fusion Protein Expressed in Vitro FEBS Lett. 1997, 407 (2) 235-238 10.1016/S0014-5793(97)00359-1
56. Pierce, D. W.; Hom-Booher, N.; Vale, R. D. Imaging Individual Green Fluorescent Proteins Nature 1997, 388 (6640) 338-338 10.1038/41009
57. Axelrod, D. Cell-Substrate Contacts Illuminated by Total Internal Reflection Fluorescence J. Cell Biol. 1981, 89 (1) 141-145 10.1083/jcb.89.1.141
58. Axelrod, D.; Burghardt, T. P.; Thompson, N. L. Total Internal Reflection Fluorescence Annu. Rev. Biophys. Bioeng. 1984, 13, 247-268 10.1146/annurev.bb.13.060184.001335
59. Kusumi, A.; Tsunoyama, T. A.; Hirosawa, K. M.; Kasai, R. S.; Fujiwara, T. K. Tracking Single Molecules at Work in Living Cells Nat. Chem. Biol. 2014, 10 (7) 524-532 10.1038/nchembio.1558
60. Saxton, M. J.; Jacobson, K. Single-Particle Tracking: Applications to Membrane Dynamics Annu. Rev. Biophys. Biomol. Struct. 1997, 26, 373-399 10.1146/annurev.biophys.26.1.373
61. Betzig, E.; Patterson, G. H.; Sougrat, R.; Lindwasser, O. W.; Olenych, S.; Bonifacino, J. S.; Davidson, M. W.; Lippincott-Schwartz, J.; Hess, H. F. Imaging Intracellular Fluorescent Proteins at Nanometer Resolution Science 2006, 313 (5793) 1642-1645 10.1126/science.1127344
62. Hess, S. T.; Gould, T. J.; Gudheti, M. V.; Maas, S. A.; Mills, K. D.; Zimmerberg, J. Dynamic Clustered Distribution of Hemagglutinin Resolved at 40 Nm in Living Cell Membranes Discriminates between Raft Theories Proc. Natl. Acad. Sci. U. S. A. 2007, 104 (44) 17370-17375 10.1073/pnas.0708066104
63. Bates, W. M.; Huang, B.; Dempsey, G. T.; Zhuang, X. Multicolor Super-Resolution Imaging with Photo-Switchable Fluorescent Probes Science 2007, 317 (5845) 1749-1753 10.1126/science.1146598
64. Huang, B.; Wang, W.; Bates, M.; Zhuang, X. Three-Dimensional Super-Resolution Imaging by Stochastic Optical Reconstruction Microscopy Science 2008, 319 (5864) 810-813 10.1126/science.1153529
65. Sharonov, A.; Hochstrasser, R. M. Wide-Field Subdiffraction Imaging by Accumulated Binding of Diffusing Probes Proc. Natl. Acad. Sci. U. S. A. 2006, 103 (50) 18911-18916 10.1073/pnas.0609643104
66. Manley, S.; Gillette, J. M.; Patterson, G. H.; Shroff, H.; Hess, H. F.; Betzig, E.; Lippincott-Schwartz, J. High-Density Mapping of Single-Molecule Trajectories with Photoactivated Localization Microscopy Nat. Methods 2008, 5 (2) 155-157 10.1038/nmeth.1176
67. Shroff, H.; Galbraith, C. G.; Galbraith, J. A.; Betzig, E. Live-Cell Photoactivated Localization Microscopy of Nanoscale Adhesion Dynamics Nat. Methods 2008, 5 (5) 417-423 10.1038/nmeth.1202
68. Eggeling, C.; Ringemann, C.; Medda, R.; Schwarzmann, G.; Sandhoff, K.; Polyakova, S.; Belov, V. N.; Hein, B.; von Middendorff, C.; Schönle, A. et al. Direct Observation of the Nanoscale Dynamics of Membrane Lipids in a Living Cell Nature 2009, 457 (7233) 1159-1162 10.1038/nature07596
69. Lefkowitz, R. J.; Shenoy, S. K. Transduction of Receptor Signals by ß-Arrestins Science 2005, 308 (5721) 512-517 10.1126/science.1109237
70. Kobilka, B. K.; Deupi, X. Conformational Complexity of G-Protein-Coupled Receptors Trends Pharmacol. Sci. 2007, 28 (8) 397-406 10.1016/j.tips.2007.06.003
71. Sounier, R.; Mas, C.; Steyaert, J.; Laeremans, T.; Manglik, A.; Huang, W.; Kobilka, B. K.; Déméné, H.; Granier, S. Propagation of Conformational Changes during μ-Opioid Receptor Activation Nature 2015, 524 (7565) 375-378 10.1038/nature14680
72. Rosenbaum, D. M.; Rasmussen, S. G. F.; Kobilka, B. K. The Structure and Function of G-Protein-Coupled Receptors Nature 2009, 459 (7245) 356-363 10.1038/nature08144
73. Tian, H.; Fürstenberg, A.; Huber, T. Labeling and Single-Molecule Methods To Monitor G Protein-Coupled Receptor Dynamics Chem. Rev. 2017, 117, 186-245 10.1021/acs.chemrev.6b00084
74. Bouvier, M. Oligomerization of G-Protein-Coupled Transmitter Receptors Nat. Rev. Neurosci. 2001, 2 (4) 274-286 10.1038/35067575
75. Szidonya, L.; Cserzö, M.; Hunyady, L. Dimerization and Oligomerization of G-Protein-Coupled Receptors: Debated Structures with Established and Emerging Functions J. Endocrinol. 2008, 196 (3) 435-453 10.1677/JOE-07-0573
76. Ianoul, A.; Grant, D. D.; Rouleau, Y.; Bani-Yaghoub, M.; Johnston, L. J.; Pezacki, J. P. Imaging Nanometer Domains of Beta-Adrenergic Receptor Complexes on the Surface of Cardiac Myocytes Nat. Chem. Biol. 2005, 1 (4) 196-202 10.1038/nchembio726
77. Annibale, P.; Vanni, S.; Scarselli, M.; Rothlisberger, U.; Radenovic, A. Quantitative Photo Activated Localization Microscopy: Unraveling the Effects of Photoblinking PLoS One 2011, 6 (7) e22678 10.1371/journal.pone.0022678
78. Scarselli, M.; Annibale, P.; Radenovic, A. Cell Type-Specific β2-Adrenergic Receptor Clusters Identified Using Photoactivated Localization Microscopy Are Not Lipid Raft Related, but Depend on Actin Cytoskeleton Integrity J. Biol. Chem. 2012, 287 (20) 16768-16780 10.1074/jbc.M111.329912
79. Calebiro, D.; Rieken, F.; Wagner, J.; Sungkaworn, T.; Zabel, U.; Borzi, A.; Cocucci, E.; Zürn, A.; Lohse, M. J. Single-Molecule Analysis of Fluorescently Labeled G-Protein-coupled Receptors Reveals Complexes with Distinct Dynamics and Organization Proc. Natl. Acad. Sci. U. S. A. 2013, 110 (2) 743-748 10.1073/pnas.1205798110
80. Kasai, R. S.; Kusumi, A. Single-Molecule Imaging Revealed Dynamic GPCR Dimerization Curr. Opin. Cell Biol. 2014, 27, 78-86 10.1016/j.ceb.2013.11.008
81. Valentine, C. D.; Haggie, P. M. Confinement of β(1)- and β(2)-Adrenergic Receptors in the Plasma Membrane of Cardiomyocyte-like H9c2 Cells Is Mediated by Selective Interactions with PDZ Domain and A-Kinase Anchoring Proteins but Not Caveolae Mol. Biol. Cell 2011, 22 (16) 2970-2982 10.1091/mbc.E11-01-0034
82. Lee, S. F.; Vérolet, Q.; Fürstenberg, A. Improved Super-Resolution Microscopy with Oxazine Fluorophores in Heavy Water Angew. Chem., Int. Ed. 2013, 52 (34) 8948-8951 10.1002/anie.201302341
83. Tarancón Díez, L. T.; Bönsch, C.; Malkusch, S.; Truan, Z.; Munteanu, M.; Heilemann, M.; Hartley, O.; Endesfelder, U.; Fürstenberg, A. Coordinate-Based Co-Localization-Mediated Analysis of Arrestin Clustering upon Stimulation of the C-C Chemokine Receptor 5 with RANTES/CCL5 Analogues Histochem. Cell Biol. 2014, 142 (1) 69-77 10.1007/s00418-014-1206-1
84. Truan, Z.; Tarancón Díez, L.; Bönsch, C.; Malkusch, S.; Endesfelder, U.; Munteanu, M.; Hartley, O.; Heilemann, M.; Fürstenberg, A. Quantitative Morphological Analysis of arrestin2 Clustering upon G Protein-Coupled Receptor Stimulation by Super-Resolution Microscopy J. Struct. Biol. 2013, 184 (2) 329-334 10.1016/j.jsb.2013.09.019
85. Dudok, B.; Barna, L.; Ledri, M.; Szabó, S. I.; Szabadits, E.; Pintér, B.; Woodhams, S. G.; Henstridge, C. M.; Balla, G. Y.; Nyilas, R. et al. Cell-Specific STORM Super-Resolution Imaging Reveals Nanoscale Organization of Cannabinoid Signaling Nat. Neurosci. 2015, 18 (1) 75-86 10.1038/nn.3892
86. Jonas, K. C.; Fanelli, F.; Huhtaniemi, I. T.; Hanyaloglu, A. C. Single Molecule Analysis of Functionally Asymmetric G Protein-Coupled Receptor (GPCR) Oligomers Reveals Diverse Spatial and Structural Assemblies J. Biol. Chem. 2015, 290 (7) 3875-3892 10.1074/jbc.M114.622498
87. Huppa, J. B.; Davis, M. M. T-Cell-Antigen Recognition and the Immunological Synapse Nat. Rev. Immunol. 2003, 3 (12) 973-983 10.1038/nri1245
88. Yuseff, M.-I.; Pierobon, P.; Reversat, A.; Lennon-Duménil, A.-M. How B Cells Capture, Process and Present Antigens: A Crucial Role for Cell Polarity Nat. Rev. Immunol. 2013, 13 (7) 475-486 10.1038/nri3469
89. Lillemeier, B. F.; Mörtelmaier, M. A.; Forstner, M. B.; Huppa, J. B.; Groves, J. T.; Davis, M. M. TCR and Lat Are Expressed on Separate Protein Islands on T Cell Membranes and Concatenate during Activation Nat. Immunol. 2010, 11 (1) 90-96 10.1038/ni.1832
90. Sherman, E.; Barr, V.; Manley, S.; Patterson, G.; Balagopalan, L.; Akpan, I.; Regan, C. K.; Merrill, R. K.; Sommers, C. L.; Lippincott-Schwartz, J. et al. Functional Nanoscale Organization of Signaling Molecules Downstream of the T Cell Antigen Receptor Immunity 2011, 35 (5) 705-720 10.1016/j.immuni.2011.10.004
91. Pageon, S. V.; Tabarin, T.; Yamamoto, Y.; Ma, Y.; Bridgeman, J. S.; Cohnen, A.; Benzing, C.; Gao, Y.; Crowther, M. D.; Tungatt, K. et al. Functional Role of T-Cell Receptor Nanoclusters in Signal Initiation and Antigen Discrimination Proc. Natl. Acad. Sci. U. S. A. 2016, 113 (37) E5454-E5463 10.1073/pnas.1607436113
92. Williamson, D. J.; Owen, D. M.; Rossy, J.; Magenau, A.; Wehrmann, M.; Gooding, J. J.; Gaus, K. Pre-Existing Clusters of the Adaptor Lat Do Not Participate in Early T Cell Signaling Events Nat. Immunol. 2011, 12 (7) 655-662 10.1038/ni.2049
93. Hu, Y. S.; Cang, H.; Lillemeier, B. F. Superresolution Imaging Reveals Nanometer- and Micrometer-Scale Spatial Distributions of T-Cell Receptors in Lymph Nodes Proc. Natl. Acad. Sci. U. S. A. 2016, 113 (26) 7201-7206 10.1073/pnas.1512331113
94. Mattila, P. K.; Batista, F. D.; Treanor, B. Dynamics of the Actin Cytoskeleton Mediates Receptor Cross Talk: An Emerging Concept in Tuning Receptor Signaling J. Cell Biol. 2016, 212 (3) 267-280 10.1083/jcb.201504137
95. Maity, P. C.; Blount, A.; Jumaa, H.; Ronneberger, O.; Lillemeier, B. F.; Reth, M. B. Cell Antigen Receptors of the IgM and IgD Classes Are Clustered in Different Protein Islands That Are Altered during B Cell Activation Sci. Signaling 2015, 8 (394) ra93-ra93 10.1126/scisignal.2005887
96. Mattila, P. K.; Feest, C.; Depoil, D.; Treanor, B.; Montaner, B.; Otipoby, K. L.; Carter, R.; Justement, L. B.; Bruckbauer, A.; Batista, F. D. The Actin and Tetraspanin Networks Organize Receptor Nanoclusters to Regulate B Cell Receptor-Mediated Signaling Immunity 2013, 38 (3) 461-474 10.1016/j.immuni.2012.11.019
97. Lee, J.; Sengupta, P.; Brzostowski, J.; Lippincott-Schwartz, J.; Pierce, S. K. The Nanoscale Spatial Organization of B Cell Receptors on IgM- and IgG-Expressing Human B Cells Mol. Biol. Cell 2016, 10.1091/mbc.E16-06-0452
98. Pore, D.; Parameswaran, N.; Matsui, K.; Stone, M. B.; Saotome, I.; McClatchey, A. I.; Veatch, S. L.; Gupta, N. Ezrin Tunes the Magnitude of Humoral Immunity J. Immunol. 2013, 191 (8) 4048 10.4049/jimmunol.1301315
99. Stone, M. B.; Veatch, S. L. Steady-State Cross-Correlations for Live Two-Colour Super-Resolution Localization Data Sets Nat. Commun. 2015, 6, 7347 10.1038/ncomms8347
100. Stone, M. B.; Shelby, S. A.; Nunez, M. F.; Wisser, K.; Veatch, S. L. eLife 2017, 10.7554/eLife.19891
101. Andrews, N. L.; Lidke, K. A.; Pfeiffer, J. R.; Burns, A. R.; Wilson, B. S.; Oliver, J. M.; Lidke, D. S. Actin Restricts FcϵRI Diffusion and Facilitates Antigen-Induced Receptor Immobilization Nat. Cell Biol. 2008, 10 (8) 955-963 10.1038/ncb1755
102. Feder, T. J.; Brust-Mascher, I.; Slattery, J. P.; Baird, B.; Webb, W. W. Constrained Diffusion or Immobile Fraction on Cell Surfaces: A New Interpretation Biophys. J. 1996, 70 (6) 2767-2773 10.1016/S0006-3495(96)79846-6
103. Larson, D. R.; Gosse, J. A.; Holowka, D. A.; Baird, B. A.; Webb, W. W. Temporally Resolved Interactions between Antigen-Stimulated IgE Receptors and Lyn Kinase on Living Cells J. Cell Biol. 2005, 171 (3) 527-536 10.1083/jcb.200503110
104. Schwartz, S. L.; Yan, Q.; Telmer, C. A.; Lidke, K. A.; Bruchez, M. P.; Lidke, D. S. Fluorogen-Activating Proteins Provide Tunable Labeling Densities for Tracking FcϵRI Independent of IgE ACS Chem. Biol. 2015, 10 (2) 539-546 10.1021/cb5005146
105. Shelby, S. A.; Holowka, D.; Baird, B.; Veatch, S. L. Distinct Stages of Stimulated FcϵRI Receptor Clustering and Immobilization Are Identified through Superresolution Imaging Biophys. J. 2013, 105 (10) 2343-2354 10.1016/j.bpj.2013.09.049
106. Szent-Gyorgyi, C.; Schmidt, B. F.; Schmidt, B. A.; Creeger, Y.; Fisher, G. W.; Zakel, K. L.; Adler, S.; Fitzpatrick, J. A. J.; Woolford, C. A.; Yan, Q. et al. Fluorogen-Activating Single-Chain Antibodies for Imaging Cell Surface Proteins Nat. Biotechnol. 2008, 26 (2) 235-240 10.1038/nbt1368
107. Shelby, S. A.; Veatch, S. L.; Holowka, D. A.; Baird, B. A. Functional Nanoscale Coupling of Lyn Kinase with IgE-FcϵRI Is Restricted by the Actin Cytoskeleton in Early Antigen-Stimulated Signaling Mol. Biol. Cell 2016, 27, 3645 10.1091/mbc.E16-06-0425
108. Irving, A. T.; Mimuro, H.; Kufer, T. A.; Lo, C.; Wheeler, R.; Turner, L. J.; Thomas, B. J.; Malosse, C.; Gantier, M. P.; Casillas, L. N. et al. The Immune Receptor NOD1 and Kinase RIP2 Interact with Bacterial Peptidoglycan on Early Endosomes to Promote Autophagy and Inflammatory Signaling Cell Host Microbe 2014, 15 (5) 623-635 10.1016/j.chom.2014.04.001
109. Itano, M. S.; Steinhauer, C.; Schmied, J. J.; Forthmann, C.; Liu, P.; Neumann, A. K.; Thompson, N. L.; Tinnefeld, P.; Jacobson, K. Super-Resolution Imaging of C-Type Lectin and Influenza Hemagglutinin Nanodomains on Plasma Membranes Using Blink Microscopy Biophys. J. 2012, 102 (7) 1534-1542 10.1016/j.bpj.2012.02.022
110. Notelaers, K.; Rocha, S.; Paesen, R.; Swinnen, N.; Vangindertael, J.; Meier, J. C.; Rigo, J.-M.; Ameloot, M.; Hofkens, J. Membrane Distribution of the Glycine Receptor α3 Studied by Optical Super-Resolution Microscopy Histochem. Cell Biol. 2014, 142 (1) 79-90 10.1007/s00418-014-1197-y
111. Winckler, P.; Lartigue, L.; Giannone, G.; De Giorgi, F.; Ichas, F.; Sibarita, J.-B.; Lounis, B.; Cognet, L. Identification and Super-Resolution Imaging of Ligand-Activated Receptor Dimers in Live Cells Sci. Rep. 2013, 3, 2387 10.1038/srep02387
112. Gao, J.; Wang, Y.; Cai, M.; Pan, Y.; Xu, H.; Jiang, J.; Ji, H.; Wang, H. Mechanistic Insights into EGFR Membrane Clustering Revealed by Super-Resolution Imaging Nanoscale 2015, 7 (6) 2511-2519 10.1039/C4NR04962D
113. Brown, A. C. N.; Oddos, S.; Dobbie, I. M.; Alakoskela, J.-M.; Parton, R. M.; Eissmann, P.; Neil, M. A. A.; Dunsby, C.; French, P. M. W.; Davis, I. et al. Remodelling of Cortical Actin Where Lytic Granules Dock at Natural Killer Cell Immune Synapses Revealed by Super-Resolution Microscopy PLoS Biol. 2011, 9 (9) e1001152 10.1371/journal.pbio.1001152
114. Brown, A. C. N.; Dobbie, I. M.; Alakoskela, J.-M.; Davis, I.; Davis, D. M. Super-Resolution Imaging of Remodeled Synaptic Actin Reveals Different Synergies between NK Cell Receptors and Integrins Blood 2012, 120 (18) 3729-3740 10.1182/blood-2012-05-429977
115. Reynwar, B. J.; Illya, G.; Harmandaris, V. A.; Müller, M. M.; Kremer, K.; Deserno, M. Aggregation and Vesiculation of Membrane Proteins by Curvature-Mediated Interactions Nature 2007, 447 (7143) 461-464 10.1038/nature05840
116. Levental, I.; Veatch, S. L. The Continuing Mystery of Lipid Rafts J. Mol. Biol. 2016, 428, 4749 10.1016/j.jmb.2016.08.022
117. Bridges, A. A.; Jentzsch, M. S.; Oakes, P. W.; Occhipinti, P.; Gladfelter, A. S. Micron-Scale Plasma Membrane Curvature Is Recognized by the Septin Cytoskeleton J. Cell Biol. 2016, 213, 23 10.1083/jcb.201512029
118. Lee, I.-H.; Kai, H.; Carlson, L.-A.; Groves, J. T.; Hurley, J. H. Negative Membrane Curvature Catalyzes Nucleation of Endosomal Sorting Complex Required for Transport (ESCRT)-III Assembly Proc. Natl. Acad. Sci. U. S. A. 2015, 112 (52) 15892-15897 10.1073/pnas.1518765113
119. Parton, R. G.; Simons, K. The Multiple Faces of Caveolae Nat. Rev. Mol. Cell Biol. 2007, 8 (3) 185-194 10.1038/nrm2122
120. Gabor, K. A.; Kim, D.; Kim, C. H.; Hess, S. T. Nanoscale Imaging of Caveolin-1 Membrane Domains In Vivo PLoS One 2015, 10 (2) e0117225 10.1371/journal.pone.0117225
121. Gabor, K. A.; Stevens, C. R.; Pietraszewski, M. J.; Gould, T. J.; Shim, J.; Yoder, J. A.; Lam, S. H.; Gong, Z.; Hess, S. T.; Kim, C. H. Super Resolution Microscopy Reveals That Caveolin-1 Is Required for Spatial Organization of CRFB1 and Subsequent Antiviral Signaling in Zebrafish PLoS One 2013, 8 (7) e68759 10.1371/journal.pone.0068759
122. Grover, J. R.; Llewellyn, G. N.; Soheilian, F.; Nagashima, K.; Veatch, S. L.; Ono, A. Roles Played by Capsid-Dependent Induction of Membrane Curvature and Gag-ESCRT Interactions in Tetherin Recruitment to HIV-1 Assembly Sites J. Virol. 2013, 87, 4650 10.1128/JVI.03526-12
123. Shim, S.-H.; Xia, C.; Zhong, G.; Babcock, H. P.; Vaughan, J. C.; Huang, B.; Wang, X.; Xu, C.; Bi, G.-Q.; Zhuang, X. Super-Resolution Fluorescence Imaging of Organelles in Live Cells with Photoswitchable Membrane Probes Proc. Natl. Acad. Sci. U. S. A. 2012, 109, 13978 10.1073/pnas.1201882109
124. Maarouf, A.; Meerschaert, R. L.; Kelly, C. Revealing Nanoscale Membrane Curvature with Polarized Localization Microscopy. Novel Techniques in Microscopy 2015; Optical Society of America: 2015; paper NM3C.2. DOI: 10.1364/NTM.2015.NM3C.2.
125. Anantharam, A.; Onoa, B.; Edwards, R. H.; Holz, R. W.; Axelrod, D. Localized Topological Changes of the Plasma Membrane upon Exocytosis Visualized by Polarized TIRFM J. Cell Biol. 2010, 188 (3) 415-428 10.1083/jcb.200908010
126. Brameshuber, M.; Weghuber, J.; Ruprecht, V.; Gombos, I.; Horváth, I.; Vigh, L.; Eckerstorfer, P.; Kiss, E.; Stockinger, H.; Schütz, G. J. Imaging of Mobile Long-Lived Nanoplatforms in the Live Cell Plasma Membrane J. Biol. Chem. 2010, 285 (53) 41765-41771 10.1074/jbc.M110.182121
127. Hartley, J. M.; Chu, T.-W.; Peterson, E. M.; Zhang, R.; Yang, J.; Harris, J.; Kopeček, J. Super-Resolution Imaging and Quantitative Analysis of Membrane Protein/Lipid Raft Clustering Mediated by Cell-Surface Self-Assembly of Hybrid Nanoconjugates ChemBioChem 2015, 16 (12) 1725-1729 10.1002/cbic.201500278
128. Owen, D. M.; Rentero, C.; Rossy, J.; Magenau, A.; Williamson, D.; Rodriguez, M.; Gaus, K. PALM Imaging and Cluster Analysis of Protein Heterogeneity at the Cell Surface J. Biophotonics 2010, 3 (7) 446-454 10.1002/jbio.200900089
129. Owen, D. M.; Williamson, D. J.; Magenau, A.; Gaus, K. Sub-Resolution Lipid Domains Exist in the Plasma Membrane and Regulate Protein Diffusion and Distribution Nat. Commun. 2012, 3, 1256 10.1038/ncomms2273
130. Levental, I.; Lingwood, D.; Grzybek, M.; Coskun, U.; Simons, K. Palmitoylation Regulates Raft Affinity for the Majority of Integral Raft Proteins Proc. Natl. Acad. Sci. U. S. A. 2010, 107 (51) 22050-22054 10.1073/pnas.1016184107
131. Sengupta, P.; Jovanovic-Talisman, T.; Skoko, D.; Renz, M.; Veatch, S. L.; Lippincott-Schwartz, J. Probing Protein Heterogeneity in the Plasma Membrane Using PALM and Pair Correlation Analysis Nat. Methods 2011, 8 (11) 969-975 10.1038/nmeth.1704
132. Tobin, S. J.; Cacao, E. E.; Hong, D. W. W.; Terenius, L.; Vukojevic, V.; Jovanovic-Talisman, T. Nanoscale Effects of Ethanol and Naltrexone on Protein Organization in the Plasma Membrane Studied by Photoactivated Localization Microscopy (PALM) PLoS One 2014, 9 (2) e87225 10.1371/journal.pone.0087225
133. Milovanovic, D.; Honigmann, A.; Koike, S.; Göttfert, F.; Pähler, G.; Junius, M.; Müllar, S.; Diederichsen, U.; Janshoff, A.; Grubmüller, H. et al. Hydrophobic Mismatch Sorts SNARE Proteins into Distinct Membrane Domains Nat. Commun. 2015, 6, 5984 10.1038/ncomms6984
134. Day, C. A.; Kenworthy, A. K. Tracking Microdomain Dynamics in Cell Membranes Biochim. Biophys. Acta, Biomembr. 2009, 1788 (1) 245-253 10.1016/j.bbamem.2008.10.024
135. Kusumi, A.; Shirai, Y. M.; Koyama-Honda, I.; Suzuki, K. G. N.; Fujiwara, T. K. Hierarchical Organization of the Plasma Membrane: Investigations by Single-Molecule Tracking vs. Fluorescence Correlation Spectroscopy FEBS Lett. 2010, 584 (9) 1814-1823 10.1016/j.febslet.2010.02.047
136. Pralle, A.; Keller, P.; Florin, E.-L.; Simons, K.; Hörber, J. K. H. Sphingolipid-Cholesterol Rafts Diffuse as Small Entities in the Plasma Membrane of Mammalian Cells J. Cell Biol. 2000, 148 (5) 997-1008 10.1083/jcb.148.5.997
137. Sevcsik, E.; Brameshuber, M.; Fölser, M.; Weghuber, J.; Honigmann, A.; Schütz, G. J. GPI-Anchored Proteins Do Not Reside in Ordered Domains in the Live Cell Plasma Membrane Nat. Commun. 2015, 6, 6969 10.1038/ncomms7969
138. Honigmann, A.; Mueller, V.; Ta, H.; Schoenle, A.; Sezgin, E.; Hell, S. W.; Eggeling, C. Scanning STED-FCS Reveals Spatiotemporal Heterogeneity of Lipid Interaction in the Plasma Membrane of Living Cells Nat. Commun. 2014, 5, 5412 10.1038/ncomms6412
139. Machta, B. B.; Papanikolaou, S.; Sethna, J. P.; Veatch, S. L. Minimal Model of Plasma Membrane Heterogeneity Requires Coupling Cortical Actin to Criticality Biophys. J. 2011, 100 (7) 1668-1677 10.1016/j.bpj.2011.02.029
140. Honigmann, A.; Sadeghi, S.; Keller, J.; Hell, S. W.; Eggeling, C.; Vink, R. A Lipid Bound Actin Meshwork Organizes Liquid Phase Separation in Model Membranes eLife 2014, 3, e01671 10.7554/eLife.01671
141. Wang, Y.-H.; Slochower, D. R.; Janmey, P. A. Counterion-Mediated Cluster Formation by Polyphosphoinositides Chem. Phys. Lipids 2014, 182, 38-51 10.1016/j.chemphyslip.2014.01.001
142. van den Bogaart, G.; Meyenberg, K.; Risselada, H. J.; Amin, H.; Willig, K. I.; Hubrich, B. E.; Dier, M.; Hell, S. W.; Grubmüller, H.; Diederichsen, U. et al. Membrane Protein Sequestering by Ionic Protein-Lipid Interactions Nature 2011, 479 (7374) 552-555 10.1038/nature10545
143. Murray, D. H.; Tamm, L. K. Clustering of Syntaxin-1A in Model Membranes Is Modulated by Phosphatidylinositol 4,5-Bisphosphate and Cholesterol Biochemistry 2009, 48 (21) 4617-4625 10.1021/bi9003217
144. Wang, J.; Richards, D. A. Segregation of PIP2 and PIP3 into Distinct Nanoscale Regions within the Plasma Membrane Biol. Open 2012, 1 (9) 857-862 10.1242/bio.20122071
145. Ji, C.; Zhang, Y.; Xu, P.; Xu, T.; Lou, X. Nanoscale Landscape of Phosphoinositides Revealed by Specific Pleckstrin Homology (PH) Domains Using Single-Molecule Superresolution Imaging in the Plasma Membrane J. Biol. Chem. 2015, 290 (45) 26978-26993 10.1074/jbc.M115.663013
146. Xu, K.; Babcock, H. P.; Zhuang, X. Dual-Objective STORM Reveals Three-Dimensional Filament Organization in the Actin Cytoskeleton Nat. Methods 2012, 9 (2) 185-188 10.1038/nmeth.1841
147. Andrade, D. M.; Clausen, M. P.; Keller, J.; Mueller, V.; Wu, C.; Bear, J. E.; Hell, S. W.; Lagerholm, B. C.; Eggeling, C. Cortical Actin Networks Induce Spatio-Temporal Confinement of Phospholipids in the Plasma Membrane-a Minimally Invasive Investigation by STED-FCS Sci. Rep. 2015, 5, 11454 10.1038/srep11454
148. Mönkemöller, V.; Øie, C.; Hübner, W.; Huser, T.; McCourt, P. Multimodal Super-Resolution Optical Microscopy Visualizes the Close Connection between Membrane and the Cytoskeleton in Liver Sinusoidal Endothelial Cell Fenestrations Sci. Rep. 2015, 5, 16279 10.1038/srep16279
149. Xu, K.; Zhong, G.; Zhuang, X. Actin, Spectrin and Associated Proteins Form a Periodic Cytoskeletal Structure in Axons Science 2013, 339, 452 10.1126/science.1232251
150. D'Este, E.; Kamin, D.; Göttfert, F.; El-Hady, A.; Hell, S. W. STED Nanoscopy Reveals the Ubiquity of Subcortical Cytoskeleton Periodicity in Living Neurons Cell Rep. 2015, 10 (8) 1246-1251 10.1016/j.celrep.2015.02.007
151. Steshenko, O.; Andrade, D. M.; Honigmann, A.; Mueller, V.; Schneider, F.; Sezgin, E.; Hell, S. W.; Simons, M.; Eggeling, C. Reorganization of Lipid Diffusion by Myelin Basic Protein as Revealed by STED Nanoscopy Biophys. J. 2016, 110 (11) 2441-2450 10.1016/j.bpj.2016.04.047
152. Gudheti, M. V.; Curthoys, N. M.; Gould, T. J.; Kim, D.; Gunewardene, M. S.; Gabor, K. A.; Gosse, J. A.; Kim, C. H.; Zimmerberg, J.; Hess, S. T. Actin Mediates the Nanoscale Membrane Organization of the Clustered Membrane Protein Influenza Hemagglutinin Biophys. J. 2013, 104 (10) 2182-2192 10.1016/j.bpj.2013.03.054
153. Burnette, D. T.; Shao, L.; Ott, C.; Pasapera, A. M.; Fischer, R. S.; Baird, M. A.; Loughian, C. D.; Delanoe-Ayari, H.; Paszek, M. J.; Davidson, M. W. et al. A Contractile and Counterbalancing Adhesion System Controls the 3D Shape of Crawling Cells J. Cell Biol. 2014, 205 (1) 83-96 10.1083/jcb.201311104
154. Kanchanawong, P.; Shtengel, G.; Pasapera, A. M.; Ramko, E. B.; Davidson, M. W.; Hess, H. F.; Waterman, C. M. Nanoscale Architecture of Integrin-Based Cell Adhesions Nature 2010, 468 (7323) 580-584 10.1038/nature09621
155. Colin-York, H.; Shrestha, D.; Felce, J. H.; Waithe, D.; Moeendarbary, E.; Davis, S. J.; Eggeling, C.; Fritzsche, M. Super-Resolved Traction Force Microscopy (STFM) Nano Lett. 2016, 16 (4) 2633-2638 10.1021/acs.nanolett.6b00273
156. Metzger, H. Transmembrane Signaling: The Joy of Aggregation J. Immunol. 1992, 149 (5) 1477-1487
157. Coffman, V. C.; Wu, J.-Q. Counting Protein Molecules Using Quantitative Fluorescence Microscopy Trends Biochem. Sci. 2012, 37 (11) 499-506 10.1016/j.tibs.2012.08.002
158. Digman, M. A.; Dalal, R.; Horwitz, A. F.; Gratton, E. Mapping the Number of Molecules and Brightness in the Laser Scanning Microscope Biophys. J. 2008, 94 (6) 2320-2332 10.1529/biophysj.107.114645
159. Digman, M. A.; Wiseman, P. W.; Choi, C.; Horwitz, A. R.; Gratton, E. Stoichiometry of Molecular Complexes at Adhesions in Living Cells Proc. Natl. Acad. Sci. U. S. A. 2009, 106 (7) 2170-2175 10.1073/pnas.0806036106
160. Fricke, F.; Beaudouin, J.; Eils, R.; Heilemann, M. One, Two or Three? Probing the Stoichiometry of Membrane Proteins by Single-Molecule Localization Microscopy Sci. Rep. 2015, 5, 14072 10.1038/srep14072
161. Leake, M. C.; Chandler, J. H.; Wadhams, G. H.; Bai, F.; Berry, R. M.; Armitage, J. P. Stoichiometry and Turnover in Single, Functioning Membrane Protein Complexes Nature 2006, 443 (7109) 355-358 10.1038/nature05135
162. Shivanandan, A.; Deschout, H.; Scarselli, M.; Radenovic, A. Challenges in Quantitative Single Molecule Localization Microscopy FEBS Lett. 2014, 588 (19) 3595-3602 10.1016/j.febslet.2014.06.014
163. McEvoy, A. L.; Hoi, H.; Bates, M.; Platonova, E.; Cranfill, P. J.; Baird, M. A.; Davidson, M. W.; Ewers, H.; Liphardt, J.; Campbell, R. E. mMaple: A Photoconvertible Fluorescent Protein for Use in Multiple Imaging Modalities PLoS One 2012, 7 (12) e51314 10.1371/journal.pone.0051314
164. Nagai, T.; Ibata, K.; Park, E. S.; Kubota, M.; Mikoshiba, K.; Miyawaki, A. A Variant of Yellow Fluorescent Protein with Fast and Efficient Maturation for Cell-Biological Applications Nat. Biotechnol. 2002, 20 (1) 87-90 10.1038/nbt0102-87
165. Shaner, N. C.; Campbell, R. E.; Steinbach, P. A.; Giepmans, B. N. G.; Palmer, A. E.; Tsien, R. Y. Improved Monomeric Red, Orange and Yellow Fluorescent Proteins Derived from Discosoma Sp. Red Fluorescent Protein Nat. Biotechnol. 2004, 22 (12) 1567-1572 10.1038/nbt1037
166. Tsien, R. Y. The Green Fluorescent Protein Annu. Rev. Biochem. 1998, 67 (1) 509-544 10.1146/annurev.biochem.67.1.509
167. Wang, S.; Moffitt, J. R.; Dempsey, G. T.; Xie, X. S.; Zhuang, X. Characterization and Development of Photoactivatable Fluorescent Proteins for Single-Molecule-based Superresolution Imaging Proc. Natl. Acad. Sci. U. S. A. 2014, 111 (23) 8452-8457 10.1073/pnas.1406593111
168. Vaughan, J. C.; Jia, S.; Zhuang, X. Ultrabright Photoactivatable Fluorophores Created by Reductive Caging Nat. Methods 2012, 9 (12) 1181-1184 10.1038/nmeth.2214
169. Bhattacharjee, U.; Beck, C.; Winter, A.; Wells, C.; Petrich, J. W. Tryptophan and ATTO 590: Mutual Fluorescence Quenching and Exciplex Formation J. Phys. Chem. B 2014, 118 (29) 8471-8477 10.1021/jp412045m
170. Gruber, H. J.; Hahn, C. D.; Kada, G.; Riener, C. K.; Harms, G. S.; Ahrer, W.; Dax, T. G.; Knaus, H. G. Anomalous Fluorescence Enhancement of Cy3 and cy3.5 versus Anomalous Fluorescence Loss of Cy5 and Cy7 upon Covalent Linking to IgG and Noncovalent Binding to Avidin Bioconjugate Chem. 2000, 11 (5) 696-704 10.1021/bc000015m
171. Marmé, N.; Knemeyer, J.-P.; Sauer, M.; Wolfrum, J. Inter- and Intramolecular Fluorescence Quenching of Organic Dyes by Tryptophan Bioconjugate Chem. 2003, 14 (6) 1133-1139 10.1021/bc0341324
172. Sauer, M. Localization Microscopy Coming of Age: From Concepts to Biological Impact J. Cell Sci. 2013, 126 (16) 3505-3513 10.1242/jcs.123612
173. Ester, M.; Kriegel, H.-P.; Sander, J.; Xu, X. A Density-Based Algorithm for Discovering Clusters in Large Spatial Databases with Noise. KDD-96 Proceedings; AAAI: 1996; pp 226-231.
174. Andronov, L.; Orlov, I.; Lutz, Y.; Vonesch, J.-L.; Klaholz, B. P. ClusterViSu, a Method for Clustering of Protein Complexes by Voronoi Tessellation in Super-Resolution Microscopy Sci. Rep. 2016, 6, 24084 10.1038/srep24084
175. Bar-On, D.; Wolter, S.; van de Linde, S.; Heilemann, M.; Nudelman, G.; Nachliel, E.; Gutman, M.; Sauer, M.; Ashery, U. Super-Resolution Imaging Reveals the Internal Architecture of Nano-Sized Syntaxin Clusters J. Biol. Chem. 2012, 287 (32) 27158-27167 10.1074/jbc.M112.353250
176. Hummer, G.; Fricke, F.; Heilemann, M. Model-Independent Counting of Molecules in Single-Molecule Localization Microscopy Mol. Biol. Cell 2016, 27, 3637 10.1091/mbc.E16-07-0525
177. Rollins, G. C.; Shin, J. Y.; Bustamante, C.; Pressé, S. Stochastic Approach to the Molecular Counting Problem in Superresolution Microscopy Proc. Natl. Acad. Sci. U. S. A. 2015, 112 (2) E110-E118 10.1073/pnas.1408071112
178. Coltharp, C.; Kessler, R. P.; Xiao, J. Accurate Construction of Photoactivated Localization Microscopy (PALM) Images for Quantitative Measurements PLoS One 2012, 7 (12) e51725 10.1371/journal.pone.0051725
179. Lee, S.-H.; Shin, J. Y.; Lee, A.; Bustamante, C. Counting Single Photoactivatable Fluorescent Molecules by Photoactivated Localization Microscopy (PALM) Proc. Natl. Acad. Sci. U. S. A. 2012, 109 (43) 17436-17441 10.1073/pnas.1215175109
180. Veatch, S. L.; Machta, B. B.; Shelby, S. A.; Chiang, E. N.; Holowka, D. A.; Baird, B. A. Correlation Functions Quantify Super-Resolution Images and Estimate Apparent Clustering due to over-Counting PLoS One 2012, 7 (2) e31457 10.1371/journal.pone.0031457
181. Nieuwenhuizen, R. P. J.; Lidke, K. A.; Bates, M.; Puig, D. L.; Grünwald, D.; Stallinga, S.; Rieger, B. Measuring Image Resolution in Optical Nanoscopy Nat. Methods 2013, 10 (6) 557-562 10.1038/nmeth.2448
182. Endesfelder, U.; Finan, K.; Holden, S. J.; Cook, P. R.; Kapanidis, A. N.; Heilemann, M. Multiscale Spatial Organization of RNA Polymerase in Escherichia Coli Biophys. J. 2013, 105 (1) 172-181 10.1016/j.bpj.2013.05.048
183. Bordovsky, S. S.; Wong, C. S.; Bachand, G. D.; Stachowiak, J. C.; Sasaki, D. Y. Engineering Lipid Structure for Recognition of the Liquid Ordered Membrane Phase Langmuir 2016, 32, 12527 10.1021/acs.langmuir.6b02636
184. Alecio, M. R.; Golan, D. E.; Veatch, W. R.; Rando, R. R. Use of a Fluorescent Cholesterol Derivative to Measure Lateral Mobility of Cholesterol in Membranes Proc. Natl. Acad. Sci. U. S. A. 1982, 79 (17) 5171-5174 10.1073/pnas.79.17.5171
185. Honigmann, A.; Mueller, V.; Hell, S. W.; Eggeling, C. STED Microscopy Detects and Quantifies Liquid Phase Separation in Lipid Membranes Using a New Far-Red Emitting Fluorescent Phosphoglycerolipid Analogue Faraday Discuss. 2013, 161, 77-89 10.1039/C2FD20107K
186. McLaughlin, S.; Wang, J.; Gambhir, A.; Murray, D. PIP(2) and Proteins: Interactions, Organization, and Information Flow Annu. Rev. Biophys. Biomol. Struct. 2002, 31, 151-175 10.1146/annurev.biophys.31.082901.134259
187. Oldham, K. B. A Gouy-Chapman-Stern Model of the Double Layer at a (Metal)/(ionic Liquid) Interface J. Electroanal. Chem. 2008, 613 (2) 131-138 10.1016/j.jelechem.2007.10.017
188. Wang, Y.-H.; Collins, A.; Guo, L.; Smith-Dupont, K. B.; Gai, F.; Svitkina, T.; Janmey, P. A. Divalent Cation-Induced Cluster Formation by Polyphosphoinositides in Model Membranes J. Am. Chem. Soc. 2012, 134 (7) 3387-3395 10.1021/ja208640t
189. Seo, J. B.; Jung, S.-R.; Huang, W.; Zhang, Q.; Koh, D.-S. Charge Shielding of PIP 2 by Cations Regulates Enzyme Activity of Phospholipase C PLoS One 2015, 10 (12) e0144432 10.1371/journal.pone.0144432
190. Merritt, E. A.; Sarfaty, S.; van den Akker, F.; L'Hoir, C.; Martial, J. A.; Hol, W. G. Crystal Structure of Cholera Toxin B-Pentamer Bound to Receptor GM1 Pentasaccharide Protein Sci. 1994, 3 (2) 166-175 10.1002/pro.5560030202
191. Hammond, A. T.; Heberle, F. A.; Baumgart, T.; Holowka, D.; Baird, B.; Feigenson, G. W. Crosslinking a Lipid Raft Component Triggers Liquid Ordered-Liquid Disordered Phase Separation in Model Plasma Membranes Proc. Natl. Acad. Sci. U. S. A. 2005, 102 (18) 6320-6325 10.1073/pnas.0405654102
192. Raghunathan, K.; Wong, T. H.; Chinnapen, D. J.; Lencer, W. I.; Jobling, M. G.; Kenworthy, A. K. Glycolipid Crosslinking Is Required for Cholera Toxin to Partition Into and Stabilize Ordered Domains Biophys. J. 2016, 111 (12) 2547-2550 10.1016/j.bpj.2016.11.008
193. Cheng, P. C.; Dykstra, M. L.; Mitchell, R. N.; Pierce, S. K. A Role for Lipid Rafts in B Cell Antigen Receptor Signaling and Antigen Targeting J. Exp. Med. 1999, 190 (11) 1549-1560 10.1084/jem.190.11.1549
194. Francis, M. L.; Ryan, J.; Jobling, M. G.; Holmes, R. K.; Moss, J.; Mond, J. J. Cyclic AMP-Independent Effects of Cholera Toxin on B Cell Activation. II. Binding of Ganglioside GM1 Induces B Cell Activation J. Immunol. 1992, 148 (7) 1999-2005
195. Harder, T.; Simons, K. Clusters of Glycolipid and Glycosylphosphatidylinositol-Anchored Proteins in Lymphoid Cells: Accumulation of Actin Regulated by Local Tyrosine Phosphorylation Eur. J. Immunol. 1999, 29 (2) 556-562 10.1002/(SICI)1521-4141(199902)29:02<556::AID-IMMU556>3.0.CO;2-2
196. Maxfield, F. R.; Wüstner, D. Analysis of Cholesterol Trafficking with Fluorescent Probes Methods Cell Biol. 2012, 108, 367-393 10.1016/B978-0-12-386487-1.00017-1
197. Várnai, P.; Balla, T. Visualization of Phosphoinositides That Bind Pleckstrin Homology Domains: Calcium- and Agonist-Induced Dynamic Changes and Relationship to Myo-[3H]inositol-Labeled Phosphoinositide Pools J. Cell Biol. 1998, 143 (2) 501-510 10.1083/jcb.143.2.501
198. Ratz, M.; Testa, I.; Hell, S. W.; Jakobs, S. CRISPR/Cas9-Mediated Endogenous Protein Tagging for RESOLFT Super-Resolution Microscopy of Living Human Cells Sci. Rep. 2015, 5, 9592 10.1038/srep09592
199. Endesfelder, U.; Malkusch, S.; Flottmann, B.; Mondry, J.; Liguzinski, P.; Verveer, P. J.; Heilemann, M. Chemically Induced Photoswitching of Fluorescent Probes-A General Concept for Super-Resolution Microscopy Molecules 2011, 16 (4) 3106-3118 10.3390/molecules16043106
200. Zhang, M.; Chang, H.; Zhang, Y.; Yu, J.; Wu, L.; Ji, W.; Chen, J.; Liu, B.; Lu, J.; Liu, Y. et al. Rational Design of True Monomeric and Bright Photoactivatable Fluorescent Proteins Nat. Methods 2012, 9 (7) 727-729 10.1038/nmeth.2021
201. Axelrod, D.; Wang, M. D. Reduction-of-Dimensionality Kinetics at Reaction-Limited Cell Surface Receptors Biophys. J. 1994, 66 (3) 588-600 10.1016/S0006-3495(94)80834-3
202. McCloskey, M. A.; Poo, M. M. Rates of Membrane-Associated Reactions: Reduction of Dimensionality Revisited J. Cell Biol. 1986, 102 (1) 88-96 10.1083/jcb.102.1.88
203. Tanaka, K. A. K.; Suzuki, K. G. N.; Shirai, Y. M.; Shibutani, S. T.; Miyahara, M. S. H.; Tsuboi, H.; Yahara, M.; Yoshimura, A.; Mayor, S.; Fujiwara, T. K. et al. Membrane Molecules Mobile Even after Chemical Fixation Nat. Methods 2010, 7 (11) 865-866 10.1038/nmeth.f.314
204. Heerklotz, H. Triton Promotes Domain Formation in Lipid Raft Mixtures Biophys. J. 2002, 83 (5) 2693-2701 10.1016/S0006-3495(02)75278-8
205. Heerklotz, H.; Szadkowska, H.; Anderson, T.; Seelig, J. The Sensitivity of Lipid Domains to Small Perturbations Demonstrated by the Effect of Triton J. Mol. Biol. 2003, 329 (4) 793-799 10.1016/S0022-2836(03)00504-7
206. Fox, C. H.; Johnson, F. B.; Whiting, J.; Roller, P. P. Formaldehyde Fixation J. Histochem. Cytochem. 1985, 33 (8) 845-853 10.1177/33.8.3894502
207. Lillemeier, B. F.; Pfeiffer, J. R.; Surviladze, Z.; Wilson, B. S.; Davis, M. M. Plasma Membrane-Associated Proteins Are Clustered into Islands Attached to the Cytoskeleton Proc. Natl. Acad. Sci. U. S. A. 2006, 103 (50) 18992-18997 10.1073/pnas.0609009103
208. Sochacki, K. A.; Shtengel, G.; van Engelenburg, S. B.; Hess, H. F.; Taraska, J. W. Correlative Super-Resolution Fluorescence and Metal-Replica Transmission Electron Microscopy Nat. Methods 2014, 11 (3) 305-308 10.1038/nmeth.2816
209. Stanly, T. A.; Fritzsche, M.; Banerji, S.; García, E.; de la Serna, J. B.; Jackson, D. G.; Eggeling, C. Critical Importance of Appropriate Fixation Conditions for Faithful Imaging of Receptor Microclusters Biol. Open 2016, 5 (9) 1343-1350 10.1242/bio.019943
210. Diez, S.; Gerisch, G.; Anderson, K.; Müller-Taubenberger, A.; Bretschneider, T. Subsecond Reorganization of the Actin Network in Cell Motility and Chemotaxis Proc. Natl. Acad. Sci. U. S. A. 2005, 102 (21) 7601-7606 10.1073/pnas.0408546102
211. Moneron, G.; Medda, R.; Hein, B.; Giske, A.; Westphal, V.; Hell, S. W. Fast STED Microscopy with Continuous Wave Fiber Lasers Opt. Express 2010, 18 (2) 1302-1309 10.1364/OE.18.001302
212. Huang, F.; Hartwich, T. M. P.; Rivera-Molina, F. E.; Lin, Y.; Duim, W. C.; Long, J. J.; Uchil, P. D.; Myers, J. R.; Baird, M. A.; Mothes, W. et al. Video-Rate Nanoscopy Using sCMOS Camera-Specific Single-Molecule Localization Algorithms Nat. Methods 2013, 10 (7) 653-658 10.1038/nmeth.2488
213. Edwald, E.; Stone, M. B.; Gray, E. M.; Wu, J.; Veatch, S. L. Oxygen Depletion Speeds and Simplifies Diffusion in HeLa Cells Biophys. J. 2014, 107 (8) 1873-1884 10.1016/j.bpj.2014.08.023
214. Grotjohann, T.; Testa, I.; Reuss, M.; Brakemann, T.; Eggeling, C.; Hell, S. W.; Jakobs, S. rsEGFP2 Enables Fast RESOLFT Nanoscopy of Living Cells eLife 2012, 1, e00248 10.7554/eLife.00248
215. Guo, S.-M.; Bag, N.; Mishra, A.; Wohland, T.; Bathe, M. Bayesian Total Internal Reflection Fluorescence Correlation Spectroscopy Reveals hIAPP-Induced Plasma Membrane Domain Organization in Live Cells Biophys. J. 2014, 106 (1) 190-200 10.1016/j.bpj.2013.11.4458
216. Di Rienzo, C.; Gratton, E.; Beltram, F.; Cardarelli, F. Fast Spatiotemporal Correlation Spectroscopy to Determine Protein Lateral Diffusion Laws in Live Cell Membranes Proc. Natl. Acad. Sci. U. S. A. 2013, 110 (30) 12307-12312 10.1073/pnas.1222097110
217. Linder, S.; Kopp, P. Podosomes at a Glance J. Cell Sci. 2005, 118 (10) 2079-2082 10.1242/jcs.02390
218. Greicius, G.; Westerberg, L.; Davey, E. J.; Buentke, E.; Scheynius, A.; Thyberg, J.; Severinson, E. Microvilli Structures on B Lymphocytes: Inducible Functional Domains? Int. Immunol. 2004, 16 (2) 353-364 10.1093/intimm/dxh031
219. Majstoravich, S.; Zhang, J.; Nicholson-Dykstra, S.; Linder, S.; Friedrich, W.; Siminovitch, K. A.; Higgs, H. N. Lymphocyte Microvilli Are Dynamic, Actin-Dependent Structures That Do Not Require Wiskott-Aldrich Syndrome Protein (WASp) for Their Morphology Blood 2004, 104 (5) 1396-1403 10.1182/blood-2004-02-0437
220. Svitkina, T. M.; Bulanova, E. A.; Chaga, O. Y.; Vignjevic, D. M.; Kojima, S.; Vasiliev, J. M.; Borisy, G. G. Mechanism of Filopodia Initiation by Reorganization of a Dendritic Network J. Cell Biol. 2003, 160 (3) 409-421 10.1083/jcb.200210174
221. Shtengel, G.; Galbraith, J. A.; Galbraith, C. G.; Lippincott-Schwartz, J.; Gillette, J. M.; Manley, S.; Sougrat, R.; Waterman, C. M.; Kanchanawong, P.; Davidson, M. W. et al. Interferometric Fluorescent Super-Resolution Microscopy Resolves 3D Cellular Ultrastructure Proc. Natl. Acad. Sci. U. S. A. 2009, 106 (9) 3125-3130 10.1073/pnas.0813131106
222. Hein, B.; Willig, K. I.; Hell, S. W. Stimulated Emission Depletion (STED) Nanoscopy of a Fluorescent Protein-Labeled Organelle inside a Living Cell Proc. Natl. Acad. Sci. U. S. A. 2008, 105 (38) 14271-14276 10.1073/pnas.0807705105
223. Fu, Y.; Winter, P. W.; Rojas, R.; Wang, V.; McAuliffe, M.; Patterson, G. H. Axial Superresolution via Multiangle TIRF Microscopy with Sequential Imaging and Photobleaching Proc. Natl. Acad. Sci. U. S. A. 2016, 113 (16) 4368-4373 10.1073/pnas.1516715113
224. Parmryd, I.; Önfelt, B. Consequences of Membrane Topography FEBS J. 2013, 280 (12) 2775-2784 10.1111/febs.12209
225. Gan, W.; Roux, B. Binding Specificity of SH2 Domains: Insight from Free Energy Simulations Proteins: Struct., Funct., Genet. 2009, 74 (4) 996-1007 10.1002/prot.22209
226. Sandermann, H., Jr. High Free Energy of Lipid/protein Interaction in Biological Membranes FEBS Lett. 2002, 514 (2-3) 340-342 10.1016/S0014-5793(02)02400-6
227. Stone, M. B.; Veatch, S. L. Far-Red Organic Fluorophores Contain a Fluorescent Impurity ChemPhysChem 2014, 15 (11) 2240-2246 10.1002/cphc.201402002
228. Sezgin, E.; Chwastek, G.; Aydogan, G.; Levental, I.; Simons, K.; Schwille, P. Photoconversion of Bodipy-Labeled Lipid Analogues ChemBioChem 2013, 14 (6) 695-698 10.1002/cbic.201300038
229. Thompson, R. E.; Larson, D. R.; Webb, W. W. Precise Nanometer Localization Analysis for Individual Fluorescent Probes Biophys. J. 2002, 82 (5) 2775-2783 10.1016/S0006-3495(02)75618-X
230. Elmokadem, A.; Yu, J. Optimal Drift Correction for Superresolution Localization Microscopy with Bayesian Inference Biophys. J. 2015, 109 (9) 1772-1780 10.1016/j.bpj.2015.09.017
231. Backlund, M. P.; Arbabi, A.; Petrov, P. N.; Arbabi, E.; Saurabh, S.; Faraon, A.; Moerner, W. E. Removing Orientation-Induced Localization Biases in Single-Molecule Microscopy Using a Broadband Metasurface Mask Nat. Photonics 2016, 10 (7) 459-462 10.1038/nphoton.2016.93
232. Ries, J.; Kaplan, C.; Platonova, E.; Eghlidi, H.; Ewers, H. A Simple, Versatile Method for GFP-Based Super-Resolution Microscopy via Nanobodies Nat. Methods 2012, 9 (6) 582-584 10.1038/nmeth.1991
233. Churchman, L. S.; Ökten, Z.; Rock, R. S.; Dawson, J. F.; Spudich, J. A. Single Molecule High-Resolution Colocalization of Cy3 and Cy5 Attached to Macromolecules Measures Intramolecular Distances through Time Proc. Natl. Acad. Sci. U. S. A. 2005, 102 (5) 1419-1423 10.1073/pnas.0409487102
234. Grimm, J. B.; English, B. P.; Chen, J.; Slaughter, J. P.; Zhang, Z.; Revyakin, A.; Patel, R.; Macklin, J. J.; Normanno, D.; Singer, R. H. et al. A General Method to Improve Fluorophores for Live-Cell and Single-Molecule Microscopy Nat. Methods 2015, 12 (3) 244-250 10.1038/nmeth.3256
235. Chozinski, T. J.; Gagnon, L. A.; Vaughan, J. C. Twinkle, Twinkle Little Star: Photoswitchable Fluorophores for Super-Resolution Imaging FEBS Lett. 2014, 588 (19) 3603-3612 10.1016/j.febslet.2014.06.043
236. Lavis, L. D.; Grimm, J. B.; English, B. P.; Muthusamy, A. K.; Mehl, B. P.; Dong, P.; Brown, T. A.; Liu, Z.; Lionnet, T. Bright Photoactivatable Fluorophores for Single-Molecule Imaging bioRxiv 2016, 66779 10.1101/066779
237. Li, D.; Shao, L.; Chen, B.-C.; Zhang, X.; Zhang, M.; Moses, B.; Milkie, D. E.; Beach, J. R.; Hammer, J. A.; Pasham, M. et al. Extended-Resolution Structured Illumination Imaging of Endocytic and Cytoskeletal Dynamics Science 2015, 349 (6251) aab3500 10.1126/science.aab3500
238. Cella Zanacchi, F.; Lavagnino, Z.; Faretta, M.; Furia, L.; Diaspro, A. Light-Sheet Confined Super-Resolution Using Two-Photon Photoactivation PLoS One 2013, 8 (7) e67667 10.1371/journal.pone.0067667
239. Urban, B. E.; Yi, J.; Chen, S.; Dong, B.; Zhu, Y.; DeVries, S. H.; Backman, V.; Zhang, H. F. Super-Resolution Two-Photon Microscopy via Scanning Patterned Illumination Phys. Rev. E 2015, 91 (4) 042703 10.1103/PhysRevE.91.042703
240. McGorty, R.; Schnitzbauer, J.; Zhang, W.; Huang, B. Correction of Depth-Dependent Aberrations in 3D Single Molecule Localization and Super-Resolution Microscopy Opt. Lett. 2014, 39 (2) 275-278 10.1364/OL.39.000275
241. Liu, S.; Kromann, E. B.; Krueger, W. D.; Bewersdorf, J.; Lidke, K. A. Three Dimensional Single Molecule Localization Using a Phase Retrieved Pupilfunction Opt. Express 2013, 21 (24) 29462-29487 10.1364/OE.21.029462
242. Hanser, B. M.; Gustafsson, M. G. L.; Agard, D. A.; Sedat, J. W. Phase Retrieval for High-Numerical-Aperture Optical Systems Opt. Lett. 2003, 28 (10) 801-803 10.1364/OL.28.000801
243. Shechtman, Y.; Weiss, L. E.; Backer, A. S.; Sahl, S. J.; Moerner, W. E. Precise Three-Dimensional Scan-Free Multiple-Particle Tracking over Large Axial Ranges with Tetrapod Point Spread Functions Nano Lett. 2015, 15 (6) 4194-4199 10.1021/acs.nanolett.5b01396
244. Quirin, S.; Pavani, S. R. P.; Piestun, R. Optimal 3D Single-Molecule Localization for Superresolution Microscopy with Aberrations and Engineered Point Spread Functions Proc. Natl. Acad. Sci. U. S. A. 2012, 109 (3) 675-679 10.1073/pnas.1109011108
245. Shtengel, G.; Wang, Y.; Zhang, Z.; Goh, W. I.; Hess, H. F.; Kanchanawong, P. Imaging Cellular Ultrastructure by PALM, iPALM, and Correlative iPALM-EM Methods Cell Biol. 2014, 123, 273-294 10.1016/B978-0-12-420138-5.00015-X