On/off blinking and switching behaviour of single molecules of green fluorescent protein

Nature

Volumn 388, Issue 6640, 1997, Pages 355-358

  Dickson, Robert M ^a   Cubittt, Andrew B ^b   Tsient, Roger Y ^c   Moerner, W E ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b Aurora Biosciences Corporation   (United States)

^c HOWARD HUGHES MEDICAL INSTITUTE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

GREEN FLUORESCENT PROTEIN; MUTANT PROTEIN;

ABSORPTION SPECTROSCOPY; ARTICLE; FLUORESCENCE; JELLYFISH; MOLECULAR DYNAMICS; PRIORITY JOURNAL; SPECTRAL SENSITIVITY;

ANIMALS; ESCHERICHIA COLI; FLUORESCENCE; GREEN FLUORESCENT PROTEINS; LUMINESCENT PROTEINS; MUTATION; PHOTOCHEMISTRY; RECOMBINANT FUSION PROTEINS; SCYPHOZOA;

AEQUOREA VICTORIA; VICTORIA;

EID: 0030832226     PISSN: 00280836     EISSN: None     Source Type: Journal    
DOI: 10.1038/41048     Document Type: Article

References (30)

1. Moerner, W. E. & Kador, L. Optical detection and spectroscopy of single molecules in a solid. Phys. Rev. Lett. 62, 2535-2538 (1989).
2. Orrit, M. & Bernard, I. Single pentacene molecules detected by fluorescence excitation in a pterphenyl crystal. Phys. Rev. Lett. 65, 2716-2719 (1990).
3. Reilly, P. D. & Skinner, J. L. Spectral diffusion of single molecule fluorescence: a probe of lowfrequency localized excitations in disordered crystals. Phys, Rev. Lett. 71, 4257-4260 (1993).
4. Moerner, W. E. Examining nanoenvironments in solids on the scale of a single, isolated molecule. Science 265, 46-53 (1994).
5. Barbara, R. F. & Moerner, W. E. (eds) Single molecules and atoms. Ace. Chem. Res. 29, (1996).
6. Betzig, E. & Chichester, R. J. Single molecules observed by near-field scanning optical microscopy. Science 262, 1422-1428 (1993).
7. Ambrose, W. R, Goodwin, R M., Martin, J. C & Keller, R. A. Alterations of single molecule fluorescence lifetimes in near-field optical microscopy, Science 265, 364-367 (1994).
8. Nie, S., Chiu, D. T. & Zare, R. N. Probing individual molecules with confocal fluorescence microscopy. Science 266, 1018-1021 (1994).
9. Trautman, J. K. & Macklin, J. J. Time-resolved spectroscopy of single molecules using near-field and far-field optics. Chem. Phys, 205, 221-229 (1996).
10. Lu, H. P. & Xie, X. S. Single-molecule spectral fluctuations at room temperature. Nature 385, 143-146 (1997).
11. Funatsu, T., Harada, Y., Tokunaga, M., Saito, K. & Yanagida, T. Imaging of single fluorescent molecules and individual ATP turnovers by single myosin molecules in aqueous solution. Nature 374, 555-559 (1995).
12. Vale, R. D. et al. Direct observation of single kinesin molecules moving along microtubules. Nature 380, 451-453 (1996).
13. Schmidt, T., Schutz, G. J., Baumgartner, W., Gruber, H. J. & Schindler, H, Imaging of single molecule diffusion, Proc. Natl Acad. Sci. USA 93, 2926-2929 (1996),
14. Basché, T., Moerner, W. E., Orrit, M. & Wild, U. P. (eds) Single Molecule Optical Detection, Imaging, and Spectroscopy (Verlag-Chemie, Munich, 1997).
15. Heim, R., Prasher, D. C. & Tsien, R. Y. Wavelength mutations and posttranslational autoxidation of green fluorescent protein. Proc. Natl Acad. Sci. USA 91, 12501-12504 (1994).
16. Cubitt, A. B. et al. Understanding, improving and using green fluorescent proteins. Trends Biochem. Sci. 20, 448-455 (1995).
17. Chattoraj, M., King, B. A., Bublitz, G. U. & Boxer, S. G. Ultra-fest excited state dynamics in green fluorescent protein: Multiple states and protein transfer. Proc. Natl Acad. Sci USA 93, 8362-8367 (1996),
18. Brejc, K. et al. Structural basis for dual excitation and photoisomerization of the Aequorea victoria green fluorescent protein. Proc. Natl Acad. USA 94, 2306-2322 (1997).
19. Ormo,M. et al. Crystal structure of the Aequorea victoria green fluorescent protein- Science 273, 1392-1395 (1996).
20. Yang, F., Moss, L G. & Phillips, J. G. N. The molecular structure of green fluorescent protein. Nature Biotechnol. 14, 1246-1251 (1996).
21. Dickson, R. M., Norris, D. J., Tzeng, Y.-L & Moerner, W. E. Three dimensional imaging of single molecules in pores of poly(acrylamide) gels. Science 274, 966-969 (1996).
22. Moerner, W. E. et al. Optical probing of single molecules of terrylene in a Shpolskii matrix - a two-state single-molecule switch, J. Phys. Chem. 98, 7382-7389 (1994).
23. Stuart, J. A., Tallent, J. R., Tan, E. H. L & Birge, R. R. in Sixth Biennial IEEE Intl Nonvolatile Memory Technol. Conf. Proc. 35-51 (IEEE, Albuquerque, NM, 1996).
24. Sakmann, B. & Neher, E. Single Channel Recording (Plenum, New York, 1995).
25. Yokoe, H. & Meyer, T. Spatial dynamics of GFP-tagged proteins investigated by local fluorescence enhancement. Nature Biotechnol. 14, 1252-1256 (1996).
26. Irie, M. in Photo-reactive Materials for Ultrahigh Density Optical Memory (ed. Irie, M.) 1-12 (Elsevier Science, Amsterdam, 1994).
27. Parthenopoulos, D. A. & Rentzepis, P. M. Three-dimensional optical storage memory, Science 245, 843-845 (1989).
28. Kunkel, T. A., Roberts, J- D. & Zakour, R. A. Rapid and efficient site-specific mutagenesis without phenotypic selection. Meth. Enzymol. 154, 367-382 (1987).
29. Fawcett, J. S. & Morris, C. J. O. R. Molecular-sieve chromatography of proteins on granulated polyacrylamide gels. Separat. Sci. l, 9-26 (1966).
30. Box, G. E. P. & Jenkins, G. M. Time Series Analysis Forecasting and Control (Holden-Day, San Francisco, 1970).