Multicolor super-resolution imaging with photo-switchable fluorescent probes

Science

Volumn 317, Issue 5845, 2007, Pages 1749-1753

  Bates, Mark ^a   Huang, Bo ^b,c   Dempsey, Graham T ^b   Zhuang, Xiaowei ^a,b,c  

^a HARVARD UNIVERSITY   (United States)

^b HARVARD UNIVERSITY   (United States)

^c HARVARD UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

FLUORESCENT DYE;

COLOR; DNA; IMAGING METHOD; SPATIAL RESOLUTION; VISUALIZATION;

ACCURACY; ANALYTIC METHOD; ARTICLE; FLUORESCENCE; MAMMAL CELL; MEASUREMENT; MOLECULAR INTERACTION; MULTICOLOR STOCHASTIC OPTICAL RECONSTRUCTION MICROSCOPY; NANOCHEMISTRY; NANOTECHNOLOGY; PHOTOACTIVATION; PRIORITY JOURNAL;

ANIMALS; CARBOCYANINES; CELL LINE; CERCOPITHECUS AETHIOPS; CLATHRIN-COATED VESICLES; DNA; DNA PROBES; FLUORESCENT DYES; MICROSCOPY, FLUORESCENCE; MICROTUBULES; NANOTECHNOLOGY;

MAMMALIA;

EID: 34648826792     PISSN: 00368075     EISSN: 10959203     Source Type: Journal    
DOI: 10.1126/science.1146598     Document Type: Article

References (33)

1. L. Novotny, B. Hecht, Principles of Nano-optics (Cambridge Univ. Press, Cambridge, 2006).
2. S. W. Hell, Science 316, 1153 (2007).
3. S. W. Hell, Nat. Biotechnol. 21, 1347 (2003).
4. M. G. L. Gustafsson, Proc. Natl. Acad. Sci. U.S.A. 102, 13081 (2005).
5. M. J. Rust, M. Bates, X. Zhuang, Nat. Methods 3, 793 (2006).
6. E. Betzig et al., Science 313, 1642 (2006).
7. S. T. Hess, T. P. K. Girirajan, M. D. Mason, Biophys. J. 91, 4258 (2006).
8. A. Ponti, M. Machacek, S. L. Gupton, C. M. Waterman-Storer, G. Danuser, Science 305, 1782 (2004).
9. S. Y. Kim, Z. Gitai, A. Kinkhabwala, L. Shapiro, W. E. Moerner, Proc. Natl. Acad. Sci. U.S.A. 103, 10929 (2006).
10. A. Sharonov, R. M. Hochstrasser, Proc. Natl. Acad. Sci. U.S.A. 103, 18911 (2006).
11. L. Stryer, R. P. Haugland, Proc. Natl. Acad. Sci. U.S.A. 58, 719 (1967).
12. T. Ha et al., Proc. Natl. Acad. Sci. U.S.A. 93, 6264 (1996).
13. T. Ha et al., Proc. Natl. Acad. Sci. U.S.A. 96, 9077 (1999).
14. B. N. G. Giepmans, S. R. Adams, M. H. Ellisman, R. Y. Tsien, Science 312, 217 (2006).
15. G. Donnert et al., Biophys. J. 92, L67 (2007).
16. W. E. Moerner, M. Orrit, Science 283, 1670 (1999).
17. R. E. Thompson, D. R. Larson, W. W. Webb, Biophys. J. 82, 2775 (2002).
18. A. Yildiz et al., Science 300, 2061 (2003).
19. M. P. Gordon, T. Ha, P. R. Selvin, Proc. Natl. Acad. Sci. U.S.A. 101, 6462 (2004).
20. X. Qu, D. Wu, L. Mets, N. F. Scherer, Proc. Natl. Acad. Sci. U.S.A. 101, 11298 (2004).
21. K. A. Lidke, B. Rieger, T. M. Jovin, R. Heintzmann, Opt. Exp. 13, 7052 (2005).
22. C. Kural et al., Science 308, 1469 (2005).
23. X. L. Nan, P. A. Sims, P. Chen, X. S. Xie, J. Phys. Chem. B 109, 24220 (2005).
24. E. A. Abbondanzieri, W. J. Greenleaf, J. W. Shaevitz, R. Landick, S. M. Block, Nature 438, 460 (2005).
25. S. Dumont et al., Nature 439, 105 (2006).
26. See supporting material on Science Online.
27. M. Bates, T. R. Blosser, X. Zhuang, Phys. Rev. Lett. 94, 108101 (2005).
28. M. Heilemann, E. Margeat, R. Kasper, M. Sauer, P. Tinnefeld, J. Am. Chem. Soc. 127, 3801 (2005).
29. S. Hohng, C. Joo, T. Ha, Biophys. J. 87, 1328 (2004).
30. K. Weber, P. C. Rathke, M. Osborn, Proc. Natl. Acad. Sci. U.S.A. 75, 1820 (1978).
31. J. E. Heuser, R. G. W. Anderson, J. Cell Biol. 108, 389 (1989).
32. I. Chen, A. Y. Ting, Curr. Opin. Biotechnol. 16, 35 (2005).
33. We thank M. Rust for initial discussions of this work, W. Wang for help with analysis on dye-labeled antibodies, and S. Liu for providing some DNA constructs. This work was supported in part by the National Institutes of Health (grant GM 068518) and a Packard Science and Engineering Fellowship (to X.Z.). X.Z. is a Howard Hughes Medical Institute Investigator.