Fast STED microscopy with continuous wave fiber lasers

Optics Express

Volumn 18, Issue 2, 2010, Pages 1302-1309

  Moneron, Gael ^a   Medda, Rebecca ^a   Hein, Birka ^a   Giske, Arnold ^b   Westphal, Volker ^a   Hell, Stefan W ^a  

^a MAX PLANCK INSTITUTE FOR BIOPHYSICAL CHEMISTRY   (Germany)

^b Leica Microsystems CMS GmbH   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

FIBER LASERS; FLUORESCENCE;

BEAM SCANNING; CONTINUOUS WAVE; CONTINUOUS WAVES; ENDOPLASMIC RETICULUM; FLUORESCENT MARKERS; FLUORESCENT NANOPARTICLES; FOCAL PLANE; LIVING CELL; LOW COSTS; RESOLUTION ENHANCEMENT; SPATIAL RESOLUTION; TURN-KEY; VISIBLE RANGE; YELLOW FLUORESCENT PROTEINS;

CONTINUOUS WAVE LASERS;

ARTICLE; EQUIPMENT; EQUIPMENT DESIGN; FIBER OPTICS; FLUORESCENCE MICROSCOPY; IMAGE ENHANCEMENT; INSTRUMENTATION; LASER; REPRODUCIBILITY; SENSITIVITY AND SPECIFICITY;

EQUIPMENT DESIGN; EQUIPMENT FAILURE ANALYSIS; FIBER OPTIC TECHNOLOGY; IMAGE ENHANCEMENT; LASERS; MICROSCOPY, FLUORESCENCE; REPRODUCIBILITY OF RESULTS; SENSITIVITY AND SPECIFICITY;

EID: 75249105436     PISSN: None     EISSN: 10944087     Source Type: Journal    
DOI: 10.1364/OE.18.001302     Document Type: Article

References (22)

1. S. W. Hell, and J. Wichmann, "Breaking the diffraction resolution limit by stimulated emission: stimulatedemission-depletion fluorescence microscopy," Opt. Lett. 19(11), 780-782 (1994).
2. S. W. Hell, "Microscopy and its focal switch," Nat. Methods 6(1), 24-32 (2009).
3. T. A. Klar, and S. W. Hell, "Subdiffraction resolution in far-field fluorescence microscopy," Opt. Lett. 24(14), 954-956 (1999).
4. V. Westphal, and S. W. Hell, "Nanoscale resolution in the focal plane of an optical microscope," Phys. Rev. Lett. 94(14), 143903 (2005).
5. G. Donnert, J. Keller, R. Medda, M. A. Andrei, S. O. Rizzoli, R. Lührmann, R. Jahn, C. Eggeling, and S. W. Hell, "Macromolecular-scale resolution in biological fluorescence microscopy," Proc. Natl. Acad. Sci. U.S.A. 103(31), 11440-11445 (2006).
6. E. Rittweger, K. Y. Han, S. E. Irvine, C. Eggeling, and S. W. Hell, "STED microscopy reveals crystal colour centres with nanometric resolution," Nat. Photonics 3(3), 144-147 (2009).
7. K. I. Willig, S. O. Rizzoli, V. Westphal, R. Jahn, and S. W. Hell, "STED microscopy reveals that synaptotagmin remains clustered after synaptic vesicle exocytosis," Nature 440(7086), 935-939 (2006).
8. R. R. Kellner, C. J. Baier, K. I. Willig, S. W. Hell, and F. J. Barrantes, "Nanoscale organization of nicotinic acetylcholine receptors revealed by stimulated emission depletion microscopy," Neuroscience 144(1), 135-143 (2007).
9. A. C. Meyer, T. Frank, D. Khimich, G. Hoch, D. Riedel, N. M. Chapochnikov, Y. M. Yarin, B. Harke, S. W. Hell, A. Egner, and T. Moser, "Tuning of synapse number, structure and function in the cochlea," Nat. Neurosci. 12(4), 444-453 (2009).
10. C. Eggeling, C. Ringemann, R. Medda, G. Schwarzmann, K. Sandhoff, S. Polyakova, V. N. Belov, B. Hein, C. von Middendorff, A. Schónle, and S. W. Hell, "Direct observation of the nanoscale dynamics of membrane lipids in a living cell," Nature 457(7233), 1159-1162 (2009).
11. V. Westphal, S. O. Rizzoli, M. A. Lauterbach, D. Kamin, R. Jahn, and S. W. Hell, "Video-rate far-field optical nanoscopy dissects synaptic vesicle movement," Science 320(5873), 246-249 (2008).
12. U. V. Nágerl, K. I. Willig, B. Hein, S. W. Hell, and T. Bonhoeffer, "Live-cell imaging of dendritic spines by STED microscopy," Proc. Natl. Acad. Sci. U.S.A. 105(48), 18982-18987 (2008).
13. B. Harke, J. Keller, C. K. Ullal, V. Westphal, A. Schónle, and S. W. Hell, "Resolution scaling in STED microscopy," Opt. Express 16(6), 4154-4162 (2008).
14. K. I. Willig, B. Harke, R. Medda, and S. W. Hell, "STED microscopy with continuous wave beams," Nat. Methods 4(11), 915-918 (2007).
15. A. Schönle, Imspector Image Acquisition&Analysis Software, v0.1, (2006): http://www.imspector.de
16. O. Griesbeck, G. S. Baird, R. E. Campbell, D. A. Zacharias, and R. Y. Tsien, "Reducing the environmental sensitivity of yellow fluorescent protein," J. Biomed. Chem. 276(31), 29188-29194 (2001).
17. B. Hein, K. I. Willig, and S. W. Hell, "Stimulated emission depletion (STED) nanoscopy of a fluorescent protein-labeled organelle inside a living cell," Proc. Natl. Acad. Sci. U.S.A. 105(38), 14271-14276 (2008).
18. D. Wildanger, E. Rittweger, L. Kastrup, and S. W. Hell, "STED microscopy with a supercontinuum laser source," Opt. Express 16(13), 9614-9621 (2008).
19. D. Wildanger, J. Bückers, V. Westphal, S. W. Hell, and L. Kastrup, "A STED microscope aligned by design," Opt. Express 17(18), 16100-16110 (2009).
20. G. Donnert, C. Eggeling, and S. W. Hell, "Major signal increase in fluorescence microscopy through dark-state relaxation," Nat. Methods 4(1), 81-86 (2007).
21. G. Donnert, C. Eggeling, and S. W. Hell, "Triplet-relaxation microscopy with bunched pulsed excitation," Photochem. Photobiol. Sci. 8(4), 481-485 (2009).
22. List of fluorescent dyes used in STED microscopy: http://www.mpibpc. mpg.de/abteilungen/200/STED-Dyes.htm