Mapping the number of molecules and brightness in the laser scanning microscope

Biophysical Journal

Volumn 94, Issue 6, 2008, Pages 2320-2332

  Digman, Michelle A ^a   Dalal, Rooshin ^b   Horwitz, Alan F ^b   Gratton, Enrico ^a  

^a University of California at Irvine   (United States)

^b UNIVERSITY OF VIRGINIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

GREEN FLUORESCENT PROTEIN; PAXILLIN;

ANIMAL; ARTICLE; BIOPHYSICS; CELL ADHESION; CHEMISTRY; CHO CELL; CRICETULUS; FLUORESCENCE MICROSCOPY; HAMSTER; IMAGE PROCESSING; INSTRUMENTATION; LASER; METHODOLOGY; PROTEIN BINDING; RADIATION SCATTERING; THEORETICAL MODEL;

ANIMALS; BIOPHYSICS; CELL ADHESION; CHO CELLS; CRICETINAE; CRICETULUS; GREEN FLUORESCENT PROTEINS; IMAGE PROCESSING, COMPUTER-ASSISTED; LASERS; MICROSCOPY, FLUORESCENCE; MODELS, THEORETICAL; PAXILLIN; PROTEIN BINDING; SCATTERING, RADIATION;

EID: 44049105523     PISSN: 00063495     EISSN: 15420086     Source Type: Journal    
DOI: 10.1529/biophysj.107.114645     Document Type: Article

References (29)

1. Wiseman, P. W., C. M. Brown, D. J. Webb, B. Hebert, N. L. Johnson, J. A. Squier, M. H. Ellisman, and A. F. Horwitz. 2004. Spatial mapping of integrin interactions and dynamics during cell migration by image correlation microscopy. J. Cell Sci. 117:5521-5534.
2. Brown, C. M., and N. O. Petersen. 1998. An image correlation analysis of the distribution of clathrin associated adaptor protein (AP-2) at the plasma membrane. J. Cell Sci. 111:271-281.
3. Chen, Y., J. D. Muller, P. T. So, and E. Gratton. 1999. The photon counting histogram in fluorescence fluctuation spectroscopy. Biophys. J. 77:553-567.
4. Muller, J. D., Y. Chen, and E. Gratton. 2000. Resolving heterogeneity on the single molecular level with the photon-counting histogram. Biophys. J. 78:474-486.
5. Chen, Y., L. N. Wei, and J. D. Muller. 2003. Probing protein oligomerization in living cells with fluorescence fluctuation spectroscopy. Proc. Natl. Acad. Sci. USA. 100:15492-15497.
6. Chen, H., H. L. Puhl 3rd, S. V. Koushik, S. S. Vogel, and S. R. Ikeda. 2006. Measurement of FRET efficiency and ratio of donor to acceptor concentration in living cells. Biophys. J. 91:L39-L41.
7. Takanishi, C. L., E. A. Bykova, W. Cheng, and J. Zheng. 2006. GFP- based FRET analysis in live cells. Brain Res. 1091:132-139.
8. Meyer, B. H., J. M. Segura, K. L. Martinez, R. Hovius, N. George, K. Johnsson, and H. Vogel. 2006. FRET imaging reveals that functional neurokinin-1 receptors are monomeric and reside in membrane microdomains of live cells. Proc. Natl. Acad. Sci. USA. 103:2138-2143.
9. Brumbaugh, J., A. Schleifenbaum, A. Gasch, M. Sattler, and C. Schultz. 2006. A dual parameter FRET probe for measuring PKC and PKA activity in living cells. J. Am. Chem. Soc. 128:24-25.
10. Wichmann, O., J. Wittbrodt, and C. Schultz. 2006. A small-molecule FRET probe to monitor phospholipase A2 activity in cells and organisms. Angew. Chem. Int. Ed. Engl. 45:508-512.
11. Gertler, A., E. Biener, K. V. Ramanujan, J. Djiane, and B. Herman. 2005. Fluorescence resonance energy transfer (FRET) microscopy in living cells as a novel tool for the study of cytokine action. J. Dairy Res. 72:14-19.
12. Looger, L. L., S. Lalonde, and W. B. Frommer. 2005. Genetically encoded FRET sensors for visualizing metabolites with subcellular resolution in living cells. Plant Physiol. 138:555-557.
13. Kahn, E., A. Vejux, D. Dumas, T. Montange, F. Frouin, V. Robert, J. M. Riedinger, J. F. Stoltz, P. Gambert, A. Todd-Pokropek, and G. Lizard. 2004. FRET multiphoton spectral imaging microscopy of 7-ketocholesterol and Nile Red in U937 monocytic cells loaded with 7-ketocholesterol. Anal. Quant. Cytol. Histol. 26:304-313.
14. Ballestrem, C., and B. Geiger. 2005. Application of microscope-based FRET to study molecular interactions in focal adhesions of live cells. Methods Mol. Biol. 294:321-334.
15. Clayton, A. H., N. Klonis, S. H. Cody, and E. C. Nice. 2005. Dual-channel photobleaching FRET microscopy for improved resolution of protein association states in living cells. Eur. Biophys. J. 34:82-90.
16. Duncan, R. R., A. Bergmann, M. A. Cousin, D. K. Apps, and M. J. Shipston. 2004. Multi-dimensional time-correlated single photon counting (TCSPC) fluorescence lifetime imaging microscopy (FLIM) to detect FRET in cells. J. Microsc. 215:1-12.
17. Zal, T., and N. R. Gascoigne. 2004. Photobleaching-corrected FRET efficiency imaging of live cells. Biophys. J. 86:3923-3939.
18. Chen, Y., J. D. Mills, and A. Periasamy. 2003. Protein localization in living cells and tissues using FRET and FLIM. Differentiation. 71:528-541.
19. Tramier, M., T. Piolot, I. Gautier, V. Mignotte, J. Coppey, K. Kemnitz, C. Durieux, and M. Coppey-Moisan. 2003. Homo-FRET versus hetero FRET to probe homodimers in living cells. Methods Enzymol. 360:580-597.
20. Gautier, I., M. Tramier, C. Durieux, J. Coppey, R. B. Pansu, J. C. Nicolas, K. Kemnitz, and M. Coppey-Moisan. 2001. Homo-FRET microscopy in living cells to measure monomer-dimer transition of GFP-tagged proteins. Biophys. J. 80:3000-3008.
21. Gadella, T. W., Jr., G. N. van der Krogt, and T. Bisseling. 1999. GFP based FRET microscopy in living plant cells. Trnds Plant Sci. 4:287-291.
22. Berland, K. M., P. T. So, and E. Gratton. 1995. Two-photon fluorescence correlation spectroscopy: method and application to the intracellular environment. Biophys. J. 68:694-701.
23. Qian, H., and E. L. Elson. 1990. Distribution of molecular aggregation by analysis of fluctuation moments. Proc. Natl. Acad. Sci. USA. 87: 5479-5483.
24. Palmer, A. G., and N. L. Thompson. 1987. Molecular aggregation characterized by high order autocorrelation in fluorescence correlation spectroscopy. Biophys. J. 52:257-270.
25. Elson, E. L., and W. W. Webb. 1975. Concentration correlation spectroscopy: a new biophysical probe based on occupation number fluctuations. Annu. Rev. Biophys. Bioeng. 4:311-334.
26. Elson, E. L, and D. Magde. 1974. Fluorescence correlation spectros- copy. I. Conceptual basis and theory. Biopolymers. 13:1-27.
27. Schwille, P., J. Korlach, and W. W. Webb. 1999. Molecular dynamics in living cells observed by fluorescence correlation spectroscopy with single-molecule sensitivity on cell and model membranes. Cytometry. 36:176-182.
28. Digman, M. A., C. M. Brown, P. Sengupta, P. W. Wiseman, A. R. Horwitz, and E. Gratton. 2005. Measuring fast dynamics in solutions and cells with a laser scanning microscope. Biophys. J. 89:1317-1327.
29. Digman, M. A., M. C. Brown, A. R. Horwitz, W. W. Mantulin, and E. Gratton. 2007. Paxillin binding dynamics across adhesions measured by correlation spectroscopy. Biophys. J. In press.