Stoichiometry and turnover in single, functioning membrane protein complexes

Nature

Volumn 443, Issue 7109, 2006, Pages 355-358

  Leake, Mark C ^a   Chandler, Jennifer H ^b   Wadhams, George H ^b   Bai, Fan ^a   Berry, Richard M ^a   Armitage, Judith P ^b  

^a UNIVERSITY OF OXFORD   (United Kingdom)

^b UNIVERSITY OF OXFORD   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

BIOLOGICAL MEMBRANES; COMPLEXATION; ESCHERICHIA COLI; FLUORESCENCE; RATE CONSTANTS; STOICHIOMETRY; TORQUE;

GREEN FLUORESCENT PROTEIN (GFP-MOTB); MEMBRANE PROTEIN COMPLEXES; PHOTOBLEACHING;

PROTEINS;

FLUORESCENT DYE; GREEN FLUORESCENT PROTEIN; ION; MEMBRANE PROTEIN; MOLECULAR MOTOR; MOTB PROTEIN; PROTEIN SUBUNIT; UNCLASSIFIED DRUG;

BACTERIUM; FLUORESCENCE; MEMBRANE; PROTEIN; STOICHIOMETRY;

ARTICLE; BACTERIAL CELL WALL; BACTERIAL FLAGELLUM; BACTERIAL MEMBRANE; BLEACHING; CELL MOTILITY; COMPLEX FORMATION; CONTROLLED STUDY; ESCHERICHIA COLI; FLUORESCENCE MICROSCOPY; ION TRANSPORT; MOLECULAR DYNAMICS; NONHUMAN; PRIORITY JOURNAL; PROTEIN METABOLISM; STOICHIOMETRY; TOTAL INTERNAL REFLECTION FLUORESCENCE MICROSCOPY;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 33748926752     PISSN: 00280836     EISSN: 14764687     Source Type: Journal    
DOI: 10.1038/nature05135     Document Type: Article

References (27)

1. Macnab, R. M. in Escherichia coli and Salmonella: Cellular and Molecular Biology (ed. Neidhardt, F. C.) 123-145 (American Society for Microbiology, Washington DC, 1996).
2. Berry, R. M. & Armitage, J. P. The bacterial flagella motor. Adv. Microb. Physiol. 41, 291-337 (1999).
3. Berg, H. C. The rotary motor of bacterial flagella. Annu. Rev. Biochem. 72, 19-54 (2003).
4. Block, S. M. & Berg, H. C. Successive incorporation of force-generating units in the bacterial rotary motor. Nature 309, 470-472 (1984).
5. Blair, D. F. & Berg, H. C. Restoration of torque in defective flagellar motors. Science 242, 1678-1681 (1988).
6. Silverman, M. & Simon, M. Flagellar rotation and the mechanism of bacterial motility. Nature 249, 73-74 (1974).
7. Tsien, R. Y. The green fluorescent protein. Annu. Rev. Biochem. 67, 509-544 (1998).
8. Mashanov, G. I., Tacon, D., Peckham, M. & Molloy, J. E. The spatial and temporal dynamics of pleckstrin homology domain binding at the plasma membrane measured by imaging single molecules in live mouse myoblasts. J. Biol. Chem. 279, 15274-15280 (2004).
9. Mullineaux, C. W. & Sarcina, M. Probing the dynamics of photosynthetic membranes with fluorescence recovery after photobleaching. Trends Plant Sci. 7, 237-240 (2002).
10. Ray, N., Nenninger, A., Mullineaux, C. W. & Robinson, C. Location and mobility of twin-arginine translocase subunits in the Escherichia coli plasma membrane. J. Biol. Chem. 280, 17961-17968 (2005).
11. Goodwin, J. S. & Kenworthy, A. K. Photobleaching approaches to investigate diffusional mobility and trafficking of Ras in living cells. Methods 37, 154-164 (2005).
12. Mullineaux, C. W., Nenninger, A., Ray, N. & Robinson, C. Diffusion of green fluorescent protein in three cell environments in Escherichia coli. J. Bacteriol. 188, 3442-3448 (2006).
13. Berry, R. M., Turner, L. & Berg, H. C. Mechanical limits of bacterial flagellar motors probed by electrorotation. Biophys. J. 69, 280-286 (1995).
14. Khan, S., Dapice, M. & Reese, T. S. Effects of mot gene expression on the structure of the flagellar motor. J. Mol. Biol. 202, 575-584 (1988).
15. Reid, S. W. et al. The maximum number of torque-generating units in the flagellar motor of Escherichia coli is at least 11. Proc. Natl Acad. Sci. USA 103, 8066-8071 (2006).
16. Kojima, S. & Blair, D. F. Solubilization and purification of the MotA/MotB complex of Escherichia coli. Biochemistry 43, 26-34 (2004).
17. Sowa, Y. et al. Direct observation of steps in rotation of the bacterial flagellar motor. Nature 437, 916-919 (2005).
18. Sourjik, V. & Berg, H. C. Localization of components of the chemotaxis machinery of Escherichia coli using fluorescent protein fusions. Mol. Microbiol. 37, 740-751 (2000).
19. Turner, L., Ryu, W. S. & Berg, H. C. Real-time imaging of fluorescent flagellar filaments. J. Bacteriol. 182, 2793-2801 (2000).
20. Dickson, R. M., Cubitt, A. B., Tsien, R. Y. & Moerner, W. E. On/off blinking and switching behaviour of single molecules of green fluorescent protein. Nature 388, 355-358 (1997).
21. Chu, S. Biology and polymer physics at the single-molecule level. Phil. Trans. R. Soc. Lond. A 361, 689-698 (2003).
22. Leake, M. C., Wilson, D., Gautel, M. & Simmons, R. M. The elasticity of single titin molecules using a two-bead optical tweezers assay. Biophys. J. 87, 1112-1135 (2004).
23. Svoboda, K., Schmidt, C. F., Schnapp, B. J. & Block, S. M. Direct observation of kinesin stepping by optical trapping interferometry. Nature 365, 721-727 (1993).
24. Mashanov, G. I., Tacon, D., Knight, A. E., Peckham, M. & Molloy, J. E. Visualizing single molecules inside living cells using total internal reflection fluorescence microscopy. Methods 29, 142-152 (2003).
25. Deich, J., Judd, E. M., McAdams, H. H. & Moerner, W. E. Visualization of the movement of single histidine kinase molecules in live Caulobacter cells. Proc. Natl Acad. Sci. USA 101, 15921-15926 (2004).
26. Van Way, S. M., Hosking, E. R., Braun, T. F. & Manson, M. D. Mot protein assembly into the bacterial flagellum: a model based on mutational analysis of the motB gene. J. Mol. Biol. 297, 7-24 (2000).
27. Demot, R. & Vanderleyden, J. The C-terminal sequence conservation between OMPA-related outer membrane proteins and MotB suggests a common function in both gram-positive and gram-negative bacteria, possibly in the interaction of these domains peptidoglycan. Mol. Microbiol. 12, 333-336 (1994).