Direct observation of the nanoscale dynamics of membrane lipids in a living cell

Nature

Volumn 457, Issue 7233, 2009, Pages 1159-1162

  Eggeling, Christian ^a   Ringemann, Christian ^a   Medda, Rebecca ^a   Schwarzmann, Günter ^b   Sandhoff, Konrad ^b   Polyakova, Svetlana ^a   Belov, Vladimir N ^a   Hein, Birka ^a   Von Middendorff, Claas ^a   Schönle, Andreas ^a   Hell, Stefan W ^a  

^a MAX PLANCK INSTITUTE FOR BIOPHYSICAL CHEMISTRY   (Germany)

^b UNIVERSITY OF BONN   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

BETA CYCLODEXTRIN; CHOLESTEROL; GLYCEROPHOSPHOLIPID; GLYCOSYLPHOSPHATIDYLINOSITOL ANCHORED PROTEIN; MEMBRANE LIPID; NANOMATERIAL; PHOSPHOETHANOLAMINE; SPHINGOLIPID; SPHINGOMYELIN;

CELL ORGANELLE; LIPID; MICROSCOPY; MOLECULAR ANALYSIS; PROTEIN;

ARTICLE; CELL MEMBRANE; DEPLETION; DIFFRACTION; DIFFUSION; FLUORESCENCE; MEMBRANE TRANSPORT; MICROSCOPY; MOLECULAR MECHANICS; MOLECULAR PROBE; MOLECULAR SIZE; PRIORITY JOURNAL;

CELL LINE; CELL MEMBRANE; CELL SURVIVAL; CHOLESTEROL; DIFFUSION; EPITHELIAL CELLS; ETHANOLAMINES; GLYCOSYLPHOSPHATIDYLINOSITOLS; MEMBRANE LIPIDS; MICROSCOPY, FLUORESCENCE; NANOTECHNOLOGY; SPHINGOMYELINS; TIME FACTORS;

EID: 61349094652     PISSN: 00280836     EISSN: 14764687     Source Type: Journal    
DOI: 10.1038/nature07596     Document Type: Article

References (32)

1. Simons, K. & Ikonen, E. Functional rafts in cell membranes. Nature 387, 569-572 (1997).
2. Brown, D. A. & London, E. Structure and function of sphingolipid- and cholesterol-rich membrane rafts. J. Biol. Chem. 275, 17221-17224 (2000).
3. Fielding, C. J. Lipid Rafts and Caveolae (Wiley-VCH, 2006).
4. Jacobson, K., Mouritsen, O. G. & Anderson, G. W. Lipid rafts: at a crossroad between cell biology and physics. Nature Cell Biol. 9, 7-14 (2007).
5. Hanzal-Bayer, M. F. & Hancock, J. F. Lipid rafts and membrane traffic. FEBS Lett. 581, 2098-2104 (2007).
6. Munro, S. Lipid rafts: elusive or illusive? Cell 115, 377-388 (2003).
7. Lommerse, P. H. M., Spaink, H. P. & Schmidt, T. In vivo plasma membrane organization: results of biophysical approaches. Biochim. Biophys. Acta 1664, 119-131 (2004).
8. Hancock, J. F. Lipid rafts: contentious only from simplistic standpoints. Nature Rev. Mol. Cell Biol. 7, 456-462 (2006).
9. Shaw, A. S. Lipid rafts: now you see them, now you don't. Nature Immunol. 7, 1139-1142 (2006).
10. Hell, S. W. & Wichmann, J. Breaking the diffraction resolution limit by stimulated emission: stimulate demission depletion microscopy. Opt. Lett. 19,780-782 (1994).
11. Pike, L. J. Rafts defined: a report on the Keystone symposium on lipid rafts and cell function. J. Lipid Res. 47, 1597-1598 (2006).
12. Fujita, A. et al. Gangliosides GM1 and GM3 in the living cell membrane form clusters susceptible to cholesterol depletion and chilling. Mol. Biol. Cell 18, 2112-2122 (2007).
13. Binnig, G., Quate, C. F. & Gerber, C. Atomic force microscope. Phys. Rev. Lett. 56, 930-933 (1986).
14. Pohl, D. W., Denk, W. & Lanz, M. Optical stethoscopy: Image recording with resolution λ/20. Appl. Phys. Lett. 44, 651-653 (1984).
15. Zacharias, D. A., Violin, J. D., Newton, A. C. & Tsien, R. Y. Partitioning of lipid-modified monomeric GFPs into membrane microdomains of live cells. Science 296, 913-916 (2002).
16. Saxton, M. J. & Jacobson, K. Single particle tracking: applications to membrane dynamics. Annu. Rev. Biophys. Biomol. Struct. 26, 373-399 (1997).
17. Schütz, G. J., Kada, G., Pastushenko, V. P. & Schindler, H. Properties of lipid microdomains in a muscle cell membrane visualized by single molecule microscopy. EMBO J. 19, 892-901 (2000).
18. Fujiwara, T., Ritchie, K., Murakoshi, H., Jacobson, K. & Kusumi, A. Phospholipids undergo hop diffusion in compartmentalized cell membrane. J. Cell Biol. 157, 1071-1081 (2002).
19. Yechiel, E. & Edidin, M. Micrometer-scale domains in fibroblast plasma membranes. J. Cell Biol. 105, 755-760 (1987).
20. Feder, T. J., Brust-Mascher, I., Slattery, J. P., Baird, B. A. & Webb, W. W. Constrainted diffusion or immobile fraction on cell surfaces: a new interpretation. Biophys. J. 70, 2767-2773 (1996).
21. Fahey, P. F. et al. Lateral diffusion in planar lipid bilayers. Science 195, 305-306 (1977).
22. Schwille, P., Korlach, J. & Webb, W. W. Fluorescence correlation spectroscopy with single-molecule sensitivity on cell and model membranes. Cytometry 36, 176-182 (1999).
23. Wawrezinieck, L., Rigneault, H., Marguet, D. & Lenne, P.-F. Fluorescence correlation spectroscopy: diffusion laws to probe the submicron cell membrane organization. Biophys. J. 89, 4029-4042 (2005).
24. Wenger, J. et al. Diffusion analysis within single nanometric apertures reveals the ultrafine cell membrane organization. Biophys. J. 92, 913-919 (2007).
25. Willig, K. I., Rizzoli, S. O., Westphal, V., Jahn, R. & Hell, S. W. STED-microscopy reveals that synaptotagmin remains clustered after synaptic vesicle exocytosis. Nature 440, 935-939 (2006).
26. Hell, S. W. Far-field optical nanoscopy. Science 316, 1153-1158 (2007).
27. Magde, D., Elson, E. L. & Webb, W. W. Thermodynamic fluctuations in a reacting system - measurement by fluorescence correlation spectroscopy. Phys. Rev. Lett. 29, 705-708 (1972).
28. Osborn, M., Franke, W. W. & Weber, K. Visualization of a system of filaments 7-10 nm thick in cultured cells of an epithelioid line (PtK2) by immunofluorescence microscopy. Proc. Natl Acad. Sci. USA 74, 2490-2494 (1977).
29. Martin, O. C. & Pagano, R. C. Internalization and sorting of a fluorescent analogue of glucosylceramide to the Golgi apparatus of human skin fibroblasts: utilization of endocytic and nonendocytic transport mechanisms. J. Cell Biol. 125, 769-781 (1994).
30. Schwarzmann, G., Hofmann, P., Pütz, U. & Albrecht, B. Demonstration of direct glycosylation of nondegradable glucosylceramide analogs in cultured cells. J. Biol. Chem. 270, 21271-21276 (1995).
31. Keller, J., Schönle, A. & Hell, S. W. Efficient fluorescence inhibition patterns for RESOLFT microscopy. Opt. Express 15, 3361-3371 (2007).
32. Willig, K. I. et al. Nanoscale resolution in GFP-based microscopy. Nature Methods 3, 721-723 (2006).