Single molecule high-resolution colocalization of Cy3 and Cy5 attached to macromolecules measures intramolecular distances through time

Proceedings of the National Academy of Sciences of the United States of America

Volumn 102, Issue 5, 2005, Pages 1419-1423

  Churchman, L Stirling ^a,b   Ökten, Zeynep ^a,c   Rock, Ronald S ^a,d   Dawson, John F ^a,e   Spudich, James A ^a,f  

^a STANFORD UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b STANFORD UNIVERSITY   (United States)

^c FREIE UNIVERSITÄT BERLIN   (Germany)

^d UNIVERSITY OF CHICAGO   (United States)

^e UNIVERSITY OF GUELPH   (Canada)

^f STANFORD UNIVERSITY   (United States)

Author keywords

Centroid tracking; Dynamic conformational changes; Fluorescence; Molecular motors; Total internal reflection microscopy

Indexed keywords

FLUORESCENT DYE; MYOSIN V;

ACCURACY; ACTIN FILAMENT; ARTICLE; BLEACHING; FLUORESCENCE; FLUORESCENCE RESONANCE ENERGY TRANSFER; MACROMOLECULE; MOLECULAR PROBE; NONHUMAN; PRIORITY JOURNAL;

ANIMALS; BASE SEQUENCE; CYTOSINE; DNA; DROSOPHILA MELANOGASTER; DROSOPHILA PROTEINS; FLOW CYTOMETRY; KINETICS; MICROSCOPY; MYOSINS; OLIGODEOXYRIBONUCLEOTIDES; TIME FACTORS;

EID: 13444292841     PISSN: 00278424     EISSN: None     Source Type: Journal    
DOI: 10.1073/pnas.0409487102     Document Type: Article

References (23)

1. Weiss, S. (1999) Science 283, 1676-1683.
2. Blanchard, S. C., Kim, H. D., Gonzalez, R. L., Jr., Puglisi, J. D. & Chu, S. (2004) Proc. Natl. Acad. Sci. USA 101, 12893-12898.
3. Blanchard, S. C., Gonzalez, R. L., Kim, H. D., Chu, S. & Puglisi, J. D. (2004) Nat. Struct. Mol. Biol. 11, 1008-1014.
4. Zhuang, X., Hartley, L. E., Babcock, H. P., Russell, R., Ha, T., Hershlag, D., Chu, S. (2000) Science 288, 2048-2051.
5. Qu, X., Wu, D., Mets, L. & Scherer, N. F. (2004) Proc. Natl Acad. Sci. USA 101, 11298-11303.
6. Gordon, M. P., Ha, T. & Selvin, P. R. (2004) Proc. Natl. Acad. Sci. USA 101, 6462-6465.
7. Lacoste, T. D., Michalet, X., Pinaud, F., Chemla, D. S., Alivisatos, A. P. & Weiss, S. (2000) Proc. Natl. Acad. Sci. USA 97, 9461-9466.
8. Okten, Z., Churchman, L. S., Rock, R. S. & Spudich, J. A. (2004) Nat. Struct. Mol. Biol. 11, 884-887.
9. Yildiz, A., Forkey, J. N., McKinney, S. A., Ha, T., Goldman, Y. E. & Selvin, P. R. (2003) Science 300, 2061-2065.
10. Yildiz, A., Tomishige, M., Vale, R. D. & Selvin, P. R. (2004) Science 303, 676-678.
11. Yildiz, A., Park, H., Safer, D., Yang, Z., Chen, L. Q., Selvin, P. R. & Sweeney, H. L. (2004) J. Biol. Chem. 279, 37223-37226.
12. De La Cruz, E. M., Wells, A. L., Rosenfeld, S.S., Ostap, E. M. & Sweeney, H. L. (1999) Proc. Natl. Acad. Sci. USA 96, 13726-13731.
13. Mehta, A. D., Rock, R. S., Rief, M., Spudich, J. A., Mooseker, M. S. & Cheney, R. E. (1999) Nature 400, 590-593.
14. Rief, M., Rock, R. S., Mehta, A. D., Mooseker, M. S., Cheney, R. E. & Spudich, J. A. (2000) Proc. Natl. Acad. Sci. USA 97, 9482-9486.
15. Moore, J. R., Krementsova, E. B., Trybus, K. M. & Warshaw, D. M. (2001) J. Cell Biol. 155, 625-635.
16. Hagerman, P. J. (1988) Annu. Rev. Biophys. Biophys. Chem. 17, 265-286.
17. Purcell, T. J., Morris, C., Spudich, J. A. & Sweeney, H. L. (2002) Proc. Natl. Acad. Sci. USA 99, 14159-14164.
18. Veigel, C., Wang, F., Bartoo, M. L., Sellers, J. R. & Molloy, J. E. (2002) Nat. Cell Biol. 4, 59-65.
19. Forkey, J. N., Quinlan, M. E., Alexander Shaw, M., Corrie, J. E. & Goldman, Y. E. (2003) Nature 422, 399-404.
20. Sweeney, H. L., Rosenfeld, S. S., Brown, F., Faust, L., Smith, J., Xing, J., Stein, L. A. & Sellers, J. R. (1998) J. Biol. Chem. 273, 6262-6270.
21. Gopalakrishna, R. & Anderson, W. B. (1982) Biochem. Biophys. Res. Commun. 104, 830-836.
22. Sakamoto, T., Amitani, I., Yokota, E. & Ando, T. (2000) Biochem. Biophys. Res. Commun. 272, 586-590.
23. Goshtasby, A. (1988) Image and Vision Computing 6, 255-261.