Efficient edition of the bovine PRNP prion gene in somatic cells and IVF embryos using the CRISPR/Cas9 system

Theriogenology

Volumn 86, Issue 8, 2016, Pages 1886-1896.e1

  Bevacqua, R J ^a   Fernandez Martin R ^a   Savy, V ^a   Canel, N G ^a   Gismondi, M I ^b   Kues, W A ^c   Carlson, D F ^d   Fahrenkrug, S C ^d   Niemann, H ^c   Taboga, O A ^b   Ferraris, S ^e   Salamone, D F ^a  

^a UNIVERSIDAD DE BUENOS AIRES   (Argentina)

^b INTA/CONICET   (Argentina)

^c Institute of Farm Animal Genetics (FLI)   (Germany)

^d RECOMBINETICS INC   (United States)

^e DEPARTAMENTO DE CIENCIAS NATURALES Y ANTROPOLOGÍA   (Argentina)

Author keywords

Bovine spongiform encephalopathy; Cattle; Engineered nuclease; Gene targeting; Mad cow disease; Ruminant

Indexed keywords

PRION PROTEIN;

ANIMAL; BOVINE; CRISPR CAS SYSTEM; CYTOLOGY; EMBRYOLOGY; FETUS; FIBROBLAST; GENE EXPRESSION REGULATION; GENETIC ENGINEERING; GENETICS; IN VITRO FERTILIZATION; METABOLISM; MUTATION; VETERINARY;

ANIMALS; CATTLE; CRISPR-CAS SYSTEMS; FERTILIZATION IN VITRO; FETUS; FIBROBLASTS; GENE EXPRESSION REGULATION, DEVELOPMENTAL; GENETIC ENGINEERING; MUTATION; PRION PROTEINS;

EID: 84991229661     PISSN: 0093691X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.theriogenology.2016.06.010     Document Type: Article

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