Direct injection of CRISPR/Cas9-related mRNA into cytoplasm of parthenogenetically activated porcine oocytes causes frequent mosaicism for indel mutations

International Journal of Molecular Sciences

Volumn 16, Issue 8, 2015, Pages 17838-17856

  Sato, Masahiro ^a   Koriyama, Miyu ^a   Watanabe, Satoshi ^b   Ohtsuka, Masato ^c   Sakurai, Takayuki ^d   Inada, Emi ^e   Saitoh, Issei ^f   Nakamura, Shingo ^g   Miyoshi, Kazuchika ^a  

^a KAGOSHIMA UNIVERSITY   (Japan)

^b NATIONAL INSTITUTE OF AGROBIOLOGICAL SCIENCES   (Japan)

^c TOKAI UNIVERSITY   (Japan)

^d SHINSHU UNIVERSITY GRADUATE SCHOOL OF MEDICINE   (Japan)

^e KAGOSHIMA UNIVERSITY   (Japan)

^f NIIGATA UNIVERSITY GRADUATE SCHOOL OF MEDICAL AND DENTAL SCIENCES   (Japan)

^g NATIONAL DEFENSE MEDICAL COLLEGE   (Japan)

Author keywords

3 galactosyltransferase; Biallelic KO; CRISPR Cas9; Indel mutations; Isolectin BS I B4; Microinjection; Mosaicism; mRNA; 1; Gal epitope

Indexed keywords

ALPHA 1, 3 GALACTOSYLTRANSFERASE; BETA N ACETYLGLUCOSAMINYLGLYCOPEPTIDE BETA 1, 4 GALACTOSYLTRANSFERASE; CASPASE 9; CRISPR ASSOCIATED PROTEIN; UNCLASSIFIED DRUG; ALPHA-1,3-GALACTOSYLTRANSFERASE 1, PORCINE; GALACTOSYLTRANSFERASE; MESSENGER RNA;

ANIMAL CELL; ARTICLE; BLASTOCYST; CONTROLLED STUDY; CRISPR CAS SYSTEM; EMBRYO DEVELOPMENT; FLUORESCENCE MICROSCOPY; GENE AMPLIFICATION; GENE EXPRESSION; GENETIC ANALYSIS; IN VITRO STUDY; INDEL MUTATION; MICROINJECTION; MOSAICISM; NONHUMAN; OOCYTE; POLYMERASE CHAIN REACTION; PREGNANCY; SEQUENCE ANALYSIS; ANIMAL; CYTOPLASM; FEMALE; GENE INACTIVATION; GENE SILENCING; GENETICS; HUMAN; METABOLISM; MUTATION RATE; PARTHENOGENESIS; PIG; PROCEDURES;

SUIDAE; SUS;

ANIMALS; CRISPR-CAS SYSTEMS; CYTOPLASM; FEMALE; GALACTOSYLTRANSFERASES; GENE KNOCKOUT TECHNIQUES; GENE SILENCING; HUMANS; INDEL MUTATION; MOSAICISM; MUTATION RATE; OOCYTES; PARTHENOGENESIS; RNA, MESSENGER; SWINE;

EID: 84938861410     PISSN: 16616596     EISSN: 14220067     Source Type: Journal    
DOI: 10.3390/ijms160817838     Document Type: Article

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