Cytoplasmic injection of circular plasmids allows targeted expression in mammalian embryos

BioTechniques

Volumn 47, Issue 5, 2009, Pages 959-967

  Iqbal, Khursheed ^a,c   Barg Kues, Brigitte ^a   Broll, Sandra ^b   Bode, Jürgen ^b,d   Niemann, Heiner ^a   Kues, Wilfried A ^a  

^a FEDERAL RESEARCH INSTITUTE FOR ANIMAL HEALTH   (Germany)

^b HELMHOLTZ CENTRE FOR INFECTION RESEARCH   (Germany)

^c CITY OF HOPE NATIONAL MEDICAL CENTER   (United States)

^d HANNOVER MEDICAL SCHOOL   (Germany)

Author keywords

Cytoplasmic injection; Embryonic genome activation; Episomal plasmid; Minicircle; Zygote

Indexed keywords

CELL NUCLEUS DNA; CIRCULAR DNA;

ANIMAL CELL; ANIMAL EMBRYO; ANIMAL EXPERIMENT; ANIMAL MODEL; ARTICLE; BACTERIAL ARTIFICIAL CHROMOSOME; CONTROLLED STUDY; CYTOPLASM; DNA METHYLATION; EGG; GENE AMPLIFICATION; GENE EXPRESSION; GENE SEQUENCE; GENETIC TRANSCRIPTION; IN VITRO STUDY; NONHUMAN; PLASMID; PREIMPLANTATION EMBRYO; PROMOTER REGION; ANIMAL; CATTLE; GENE TARGETING; GENETICS; METHODOLOGY; MICROINJECTION; MOUSE; PHYSIOLOGY; PRENATAL DEVELOPMENT; TRANSGENIC MOUSE;

ANIMALIA; BACTERIA (MICROORGANISMS); BOVINAE; MAMMALIA; MURINAE;

ANIMALS; CATTLE; CYTOPLASM; DNA, CIRCULAR; EMBRYO, MAMMALIAN; GENE EXPRESSION; GENE TARGETING; MICE; MICE, TRANSGENIC; MICROINJECTIONS;

EID: 73849103275     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/000113270     Document Type: Article

References (41)

1. Piechaczek, C., C. Fetzer, A. Baiker, J. Bode, and H.J. Lipps. 1999. A vector based on the SV40 origin of replication and chromosomal S/MARs replicates episomally in CHO cells. Nucleic Acids Res. 27:426-428. (Pubitemid 29210004)
2. Bode, J., C.P. Fetzer, K. Nehlsen, M. Scinteie, B.-H. Hinrichsen, A. Baiker, C. Piechazcek, C. Benham, and H.J. Lipps. 2001. The hitchhiking principle: optimizing episomal vectors for the use in gene therapy and biotechnology. Gene Ther. Mol. Biol. 6:33-46.
3. Jenke, A.C., I.M. Stehle, F. Herrmann, T. Eisenberger, A. Baiker, J. Bode, F.O. Fackelmayer, and H.J. Lipps. 2004. Nuclear scaffold/matrix attached region modules linked to a transcription unit are sufficient for replication and maintenance of a mammalian episome. Proc. Natl. Acad. Sci. USA 101:11322-11327.
4. Schaarschmidt, D., J. Baltin, I.M. Stehle, H.J. Lipps, and R. Knippers. 2004. An episomal mammalian replicon: sequence-independent binding of the origin recognition complex. EMBO J. 23:191-201. (Pubitemid 38165759)
5. Papapetrou, E.P., P.G. Ziros, I.D. Micheva, N.C. Zoumbos, and A. Athanassiadou. 2004. Gene transfer into human hematopoietic progenitor cells with an episomal vector carrying an S/MAR element. Gene Ther. 13:40-51.
6. Jenke, A.C., M.F. Scinteie, I.M. Stehle, and H.J. Lipps. 2004. Expression of a transgene encoded on a non-viral episomal vector is not subject to epigenetic silencing by cytosine methylation. Mol. Biol. Rep. 31:85-90.
7. Manzini, S., A. Vargiolu, I.M. Stehle, M.I. Bacci, M.G. Cerrito, R. Giovannoni, A. Zannoni, M.R. Bianco, et al. 2006. Genetically modified pigs produced with a nonviral episomal vector. Proc. Natl. Acad. Sci. USA 103:17672-17677.
8. Chen, Z.Y., C.Y. He, A. Ehrhardt, and M.A. Kay. 2003. Minicircle DNA devoid of bacterial DNA result in persistent and high level transgene expression in vivo. Mol. Ther. 8:495-500.
9. Chen, Z.Y., C.Y. He, L. Meuse, and M.A. Kay. 2004. Silencing of episomal transgene expression by plasmid bacterial DNA elements in vivo. Gene Ther. 11:856-864.
10. Nehlsen, K., S. Broll, and J. Bode. 2006. Replicating minicircles: Generation of nonviral episomes for the efficient modification of dividing cells. Gene Ther. Mol. Biol. 10:233-244.
11. Gordon, J.W., G.A. Scangos, D.J. Plotkin, J.A. Barbosa, and F.H. Ruddle. 1980. Genetic transformation of mouse embryos by microinjection of purified DNA. Proc. Natl. Acad. Sci. USA 77:7380-7384.
12. Palmiter, R.D., R.L. Brinster, R.E. Hammer, M.E. Trumbauer, M.G. Rosenfeld, N.C. Birnberg, and R.M. Evans 1982. Dramatic growth of mice that develop from eggs microinjected with metallothionein-growth hormone fusion genes. Nature 300:611-615.
13. Brinster, R.L., H.Y. Chen, M.E. Trumbauer, M.K. Yagle, and R.D. Palmiter. 1985. Factors affecting the efficiency of introducing foreign DNA into mice by microinjecting eggs. Proc. Natl. Acad. Sci. USA 82:4438-4442.
14. Niemann, H. and W.A. Kues. 2007. Transgenic farm animals - an update. Reprod. Fertil. Dev. 19:762-770.
15. Hammer, R.E., V.G. Pursel, C.E. Rexroad Jr., R.J. Wall, D.J. Bolt, K.M Ebert, R.D. Palmiter, R.L. Brinster. 1985. Production of transgenic rabbits, sheep and pigs by microinjection. Nature 315:680-683.
16. Yadav, P.S., W.A. Kues, D. Herrmann, J.W. Carnwath, and H. Niemann. 2005. Bovine ICM derived cells express the Oct4 ortholog. Mol. Reprod. Dev. 72:182-190.
17. Kittler, R., et al. 2005. RNA interference rescue by bacterial artificial chromosome transgenesis in mammalian tissue culture cells. Proc. Natl. Acad. Sci. USA 102:2396-2401.
18. Schedl, A., Z. Larin, L. Montoliu, E. Thies, G. Kelsey, H. Lehrach, and G. Schütz. 1993. A method for generation of YAC transgenic mice by pronuclear microinjection. Nucleic Acids Res. 21:4783-4787. (Pubitemid 23312755)
19. Kues, W.A., R. Schwinzer, D. Wirth, E. Verhoeyen, E. Lemme, D. Herrmann, B. Barg-Kues, H. Hauser, et al. 2006. Epigenetic silencing and tissue independent expression of a novel tetracycline inducible system in double-transgenic pigs. FASEB J. 20:1200-1202.
20. Yoshimizu, T., N. Sugiyama, and M. de Felice. 1999. Germlinespecific expression of the Oct4/green fluorescent protein (GFP) transgene in mice. Dev. Growth Differ. 41:675-684.
21. Kirchhof, N., et al. 2000. Expression pattern of Oct-4 in preimplantation embryos of different species. Biol. Reprod. 63:1698-1705.
22. Kues, W.A., B. Sakmann, and V. Witzemann. 1995. Differential expression patterns of five Acetylcholine receptor subunit genes in rat muscle development. Eur. J. Neurosci. 7:1376-1385.
23. Kues, W.A., H.R. Brenner, B. Sakmann, and V. Witzemann. 1995. Local neurotrophic repression of gene transcripts encoding fetal AChR at rat neuromuscular synapses. J. Cell Biol. 130:949-957.
24. Telford, N.A., A.J. Watson, and G.A. Schultz. 1990. Transition from maternal to embryonic control in early mammalian development: a comparison of several species. Mol. Reprod. Dev. 26:90-100.
25. Kues, W.A., et al. 2008. Genome-wide expression profiling reveals distinct clusters of transcriptional regulation during bovine preimplantation development in vivo. Proc. Natl. Acad. Sci. USA 105:19768-19773.
26. Iqbal, K., W.A. Kues, and H. Niemann. 2007. Parent-of-origin dependent gene-specific knock down in mouse embryos. Biochem. Biophys. Res. Commun. 358:727-732.
27. Simonsson, S. and J. Gurdon. 2004. DNA demethylation is necessary for the epigenetic reprogramming of somatic cell nuclei. Nat. Cell Biol. 6:984-990.
28. Yamazaki, Y., et al. 2006. Gradual DNA demethylation of the Oct4 promoter in clone mouse embryos. Mol. Reprod. Dev. 73:180-188.
29. Ramsahoye, B.H., et al. 2000. Non-CpG methylation is prevalent in embryonic stem cells and may be mediated by DNA methyltransferase 3a. Proc. Natl. Acad. Sci. USA 97:5237-5242.
30. Malone, C.S., et al. 2001. CmC(A/T) GG DNA methylation in mature B cell lymphoma gene silencing. Proc. Natl. Acad. Sci. USA 98:10404-10409.
31. Imamura, T., et al. 2005. Dynamic CpG and non-CpG methylation of the Peg1/Mest Gene in the mouse oocyte and preimplantation embryo. J. Biol. Chem. 280:20171-20175.
32. Weintraub, H., P.F. Cheng, and K. Conrad. 1986. Expression of transfected DNA depends on DNA topology. Cell 46:115-122.
33. Perry, A.C., T. Wakayama, H. Kishikawa, T. Kasai, M. Okabe, Y. Toyoda, and R. Yanagimachi. 1999. Mammalian transgenesis by intracytoplasmic sperm injection. Science 284:1180-1183.
34. Chan, A.W., C.M. Luetjens, T. Dominiko, J. Ramalho-Santos, C.R. Simerly, L. Hewitson, and G. Schatten. 2000. Foreign DNA transmission by ICSI: injection of spermatozoa bound with exogenous DNA results in embryonic GFP expression and live rhesus monkey births. Mol. Hum. Reprod. 6:26-33.
35. Hoelker, M., S. Mekchay, H. Schneider, B.G. Bracket, D. Tesfaye, D. Jennen, E. Tholen, M. Gilles, et al. 2007. Quantification of DNA binding, uptake, transmission and expression in bovine sperm mediated gene transfer by RT-PCR: effect of transfection reagent and DNA architecture. Theriogenology 67:1097-1107.
36. Yeom, Y.I., G. Fuhrmann, C.E. Ovitt, A. Brehm, K. Ohbo, M. Gross, K. Hübner, and H.R. Schöler. 1996. Germline regulatory element of Oct4 specific for the totipotent cycle of embryonal cells. Development 122:881-884.
37. Wuensch, A., F.A. Habermann, S. Kurosaka, R. Klose, V. Zakhartchenko, H.D. Reichenbach, F. Sinowatz, K.J. McLaughlin, and E. Wolf. 2007. Quantitative monitoring of pluripotency gene activation after somatic cloning in cattle. Biol. Reprod. 76:983-991.
38. Gagne, M., F. Pothier, and M.A. Sirard. 1995. Effect of microinjection time point during post fertilization S phase on bovine embryo development. Mol. Reprod. Dev. 41:184-194.
39. Mayer, W., A. Niveleau, J. Walter, R. Fundele, and T. Haaf. 2000. Demethylation of the zygotic paternal genome. Nature 403:501-502.
40. Bourc'his, D., D. Le Bourhis, D. Patin, A. Niveleau, P. Comizzoli, J.P Renard, and E. Viegas-Péquignot. 2001. Delayed and incomplete reprogramming of chromosome methylation patterns in bovine cloned embryos. Curr. Biol. 11:1542-1546.
41. Kang, Y.K., D.B. Koo, J.S. Park, Y.H. Choi, A.S. Chung, K.K. Lee, and Y.M. Han. 2001. Aberrant methylation of donor genome in cloned bovine embryos. Nat. Genet. 28:173-177.