CRISPR/Cas9-based genome editing in mice by single plasmid injection

Methods in Enzymology

Volumn 546, Issue C, 2014, Pages 319-336

  Fujihara, Yoshitaka ^a   Ikawa, Masahito ^a  

^a OSAKA UNIVERSITY   (Japan)

Author keywords

Knockin; Knockout; Microinjection; Plasmid pX330 pCAG EGxxFP ssODN; Zygote

Indexed keywords

CAS9 PROTEIN; ENHANCED GREEN FLUORESCENT PROTEIN; GUIDE RNA; PROTEIN; UNCLASSIFIED DRUG; CRISPR ASSOCIATED PROTEIN; ENDONUCLEASE; GREEN FLUORESCENT PROTEIN;

ANIMAL EXPERIMENT; ARTICLE; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEAT; CONTROLLED STUDY; EMBRYO; FEMALE; GENE ACTIVITY; GENE EXPRESSION; GENE TARGETING; GENETIC TRANSFECTION; HOST; HUMAN; HUMAN CELL; IN VITRO STUDY; MICROINJECTION; MOUSE; MOUSE EMBRYO; MOUSE MUTANT; MUTAGENESIS; NONHUMAN; PLASMID; PRIORITY JOURNAL; RNA EDITING; SUPEROVULATION; WILD TYPE MOUSE; ZYGOTE; ADMINISTRATION AND DOSAGE; ANIMAL; GENETICS; HEK293 CELL LINE; METABOLISM; MUTATION; PROCEDURES;

MUS;

ANIMALS; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEATS; CRISPR-ASSOCIATED PROTEINS; ENDONUCLEASES; GENE TARGETING; GREEN FLUORESCENT PROTEINS; HEK293 CELLS; HUMANS; MICE; MICROINJECTIONS; MUTAGENESIS; MUTATION; PLASMIDS; RNA, GUIDE; TRANSFECTION; ZYGOTE;

EID: 84925405886     PISSN: 00766879     EISSN: 15577988     Source Type: Book Series    
DOI: 10.1016/B978-0-12-801185-0.00015-5     Document Type: Chapter

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