Single-step generation of rabbits carrying a targeted allele of the tyrosinase gene using CRISPR/Cas9

Experimental Animals

Volumn 64, Issue 1, 2015, Pages 31-37

  Honda, Arata ^a,b   Hirose, Michiko ^b   Sankai, Tadashi ^c   Yasmin, Lubna ^c   Yuzawa, Kazuaki ^c   Honsho, Kimiko ^a   Izu, Haruna ^a   Iguchi, Atsushi ^a   Ikawa, Masahito ^d   Ogura, Atsuo ^b  

^a UNIVERSITY OF MIYAZAKI   (Japan)

^b RIKEN BIORESOURCE CENTER   (Japan)

^c NATIONAL INSTITUTE OF BIOMEDICAL INNOVATION   (Japan)

^d OSAKA UNIVERSITY   (Japan)

Author keywords

CRISPR Cas9; Gene targeting; Rabbit; Tyrosinase

Indexed keywords

CRISPR ASSOCIATED PROTEIN; CRISPR ASSOCIATED PROTEIN 9; ENHANCED GREEN FLUORESCENT PROTEIN; GENOMIC DNA; RNA; SINGLE GUIDE RNA; UNCLASSIFIED DRUG; CASPASE 9; MONOPHENOL MONOOXYGENASE;

ANIMAL EXPERIMENT; ARTICLE; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEAT; DNA SEQUENCE; EMBRYO; FEMALE; FLUORESCENCE MICROSCOPY; GENE; GENE DISRUPTION; GENE MUTATION; GENE TARGETING; GENETIC ANALYSIS; GENETIC TRANSFECTION; GENOTYPE; MALE; NONHUMAN; POLYMERASE CHAIN REACTION; RABBIT; TYROSINASE GENE; ALLELE; ANIMAL; DISEASE MODEL; GENETICS; PLASMID; PROCEDURES; ZYGOTE;

ANIMALIA; MAMMALIA; ORYCTOLAGUS CUNICULUS;

ALLELES; ANIMALS; CASPASE 9; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEATS; DISEASE MODELS, ANIMAL; GENE TARGETING; MONOPHENOL MONOOXYGENASE; PLASMIDS; RABBITS; ZYGOTE;

EID: 84922225818     PISSN: 13411357     EISSN: None     Source Type: Journal    
DOI: 10.1538/expanim.14-0034     Document Type: Article

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