Generation of CRISPR/Cas9-mediated gene-targeted pigs via somatic cell nuclear transfer

Cellular and Molecular Life Sciences

Volumn 72, Issue 6, 2015, Pages 1175-1184

  Zhou, Xiaoqing ^a   Xin, Jige ^b,c   Fan, Nana ^a   Zou, Qingjian ^a   Huang, Jiao ^a   Ouyang, Zhen ^a   Zhao, Yu ^a   Zhao, Bentian ^a   Yi, Xiaoling ^a   Guo, Lin ^a   Esteban, Miguel A ^a   Zeng, Yangzhi ^c   Yang, Huaqiang ^a   Lai, Liangxue ^a,b  

^a GUANGZHOU INSTITUTES OF BIOMEDICINE AND HEALTH   (China)

^b JILIN UNIVERSITY   (China)

^c YUNNAN AGRICULTURAL UNIVERSITY   (China)

Author keywords

Albinism; Animal model; Genome engineering; Parkinson's disease

Indexed keywords

CRISPR ASSOCIATED NUCLEASE 9; CRISPR ASSOCIATED PROTEIN; MESSENGER RNA; MONOPHENOL MONOOXYGENASE; NUCLEASE; PARKIN; PROTEIN SERINE THREONINE KINASE; PTEN INDUCED PUTATIVE KINASE 1; RNA; SINGLE GUIDE RNA; UNCLASSIFIED DRUG; GUIDE RNA;

ALBINISM; ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; BREEDING; CELL NUCLEUS TRANSPLANTATION; CELL VIABILITY; CONTROLLED STUDY; DNA MODIFICATION; DNA SEQUENCE; EMBRYO; EXPERIMENTAL PIG; FEASIBILITY STUDY; FETUS; GENE; GENE TARGETED PIG; GENE TARGETING; GENETIC PARAMETERS; GENOTYPE; HOMOZYGOTE; INDEL MUTATION; KNOCKOUT GENE; MALE; MOLECULAR CLONING; MOLECULAR DYNAMICS; MOSAICISM; MUTATION RATE; MUTATIONAL ANALYSIS; NONHUMAN; OFF TARGET EFFECT; PARK2 GENE; PHENOTYPE; PINK1 GENE; PROTEIN EXPRESSION; RNA EDITING; SOMATIC CELL NUCLEAR TRANSFER; TYR GENE; ANIMAL; CELL CULTURE; CRISPR CAS SYSTEM; FIBROBLAST; GENE INACTIVATION; GENETIC ENGINEERING; GENETICS; METABOLISM; MOLECULAR GENETICS; MUTATION; NUCLEOTIDE SEQUENCE; PROCEDURES; SWINE;

ANIMALS; BASE SEQUENCE; CELLS, CULTURED; CRISPR-ASSOCIATED PROTEINS; CRISPR-CAS SYSTEMS; FIBROBLASTS; GENE KNOCKOUT TECHNIQUES; GENE TARGETING; GENETIC ENGINEERING; MOLECULAR SEQUENCE DATA; MUTATION; PHENOTYPE; RNA, GUIDE; SWINE;

EID: 84925519674     PISSN: 1420682X     EISSN: 14209071     Source Type: Journal    
DOI: 10.1007/s00018-014-1744-7     Document Type: Article

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