Efficient generation of myostatin knock-out sheep using CRISPR/Cas9 technology and microinjection into zygotes

PLoS ONE

Volumn 10, Issue 8, 2015, Pages

  Crispo, M ^a   Mulet, A P ^a   Tesson, L ^c   Barrera, N ^b   Cuadro, F ^b   Dos Santos Neto, P C ^b   Nguyen, T H ^c   Creneguy A ^c   Brusselle, L ^c   Anegon I ^c   Menchaca, A ^b  

^a INSTITUT PASTEUR DE MONTEVIDEO   (Uruguay)

^b Fundación Instituto de Reproduccion Animal Del Uruguay   (Uruguay)

^c Centre National de la Recherche Scientifique   (France)

Author keywords

[No Author keywords available]

Indexed keywords

MESSENGER RNA; MYOSTATIN;

ALLELE; ANIMAL EXPERIMENT; ARTICLE; BLASTOCYST; BODY GROWTH; CONTROLLED STUDY; CRISPR CAS SYSTEM; CYTOPLASM; EMBRYO; EMBRYO DEVELOPMENT; FEMALE; FRAMESHIFT MUTATION; GENETIC ENGINEERING; HETEROZYGOTE; INDEL MUTATION; KNOCKOUT SHEEP; MICROINJECTION; MOSAICISM; MSTN GENE; MUSCLE DEVELOPMENT; MUTANT; NONHUMAN; OOCYTE CLEAVAGE; PREGNANCY; SANGER SEQUENCING; SEQUENCE ANALYSIS; STOP CODON; TRANSGENIC ORGANISM; WESTERN BLOTTING; ZYGOTE; ANIMAL; DOMESTIC SHEEP; GENE INACTIVATION; GENETICS; TRANSGENIC ANIMAL;

ANIMALS; ANIMALS, GENETICALLY MODIFIED; CRISPR-CAS SYSTEMS; FEMALE; GENE KNOCKOUT TECHNIQUES; MICROINJECTIONS; MYOSTATIN; PREGNANCY; SHEEP, DOMESTIC; ZYGOTE;

EID: 84942887576     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0136690     Document Type: Article

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