Combination of the somatic cell nuclear transfer method and RNAi technology for the production of a prion gene-knockdown calf using plasmid vectors harboring the U6 or tRNA promoter

Prion

Volumn 5, Issue 1, 2011, Pages 39-46

  Wongsrikeao, Pimprapar ^a   Sutou, Shizuyo ^b   Kunishi, Miho ^b   Dong, Ya Juan ^c   Bai, Xuejin ^c   Otoi, Takeshige ^a  

^a YAMAGUCHI UNIVERSITY   (Japan)

^b SHUJITSU UNIVERSITY   (Japan)

^c QINGDAO AGRICULTURAL UNIVERSITY   (China)

Author keywords

Cattle; Knockdown; Plasmid vector; Prion gene; RNAi; Somatic cell nuclear transfer; tRNA promoter; U6 promoter

Indexed keywords

COMPLEMENTARY DNA; DNA; ENHANCED GREEN FLUORESCENT PROTEIN; MESSENGER RNA; PLASMID VECTOR; SMALL INTERFERING RNA; TRANSFER RNA;

ABORTION; ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; CALF (BOVINE); CELL CULTURE; CELL NUCLEUS TRANSPLANTATION; CONTROLLED STUDY; DEGENERATIVE DISEASE; EMBRYO; EMBRYO DEVELOPMENT; EMBRYO TRANSFER; FETUS; GENOME; LUNG; NERVOUS SYSTEM; NEUROLOGIC DISEASE; NEWBORN; NONHUMAN; PRION DISEASE; PROMOTER REGION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA INTERFERENCE; SOMATIC CELL; SOMATIC CELL NUCLEAR TRANSFER; SPLEEN; STILLBIRTH; TRANSGENIC ANIMAL; WESTERN BLOTTING;

ANIMALS; ANIMALS, GENETICALLY MODIFIED; CATTLE; CELL NUCLEUS; GENE KNOCKDOWN TECHNIQUES; GENETIC VECTORS; NUCLEAR TRANSFER TECHNIQUES; PLASMIDS; PRIONS; PROMOTER REGIONS, GENETIC; RNA INTERFERENCE; RNA, SMALL NUCLEAR; RNA, TRANSFER;

BOS; BOVINAE;

EID: 78651407721     PISSN: 19336896     EISSN: 1933690X     Source Type: Journal    
DOI: 10.4161/pri.5.1.14075     Document Type: Article

References (46)

1. Prusiner SB. Molecular biology and transgenetics of prion diseases. Crit Rev Biochem Mol Biol 1991; 26:397-438.
2. Weissmann C. Molecular biology of transmissible spongiform encephalopathies. FEBS Lett 1996; 389:3-11.
3. Sc levels but delayed onset of disease in scrapie-inoculated mice heterozygous for a disrupted PrP gene. Mol Med 1994; 1:19-30.
4. Richt JA, Kasinathan P, Hamir AN, Castilla J, Sathiyaseelan T, Vargas F, et al. Production of cattle lacking prion protein. Nat Biotechnol 2007; 25:132-8.
5. Lee NS, Dohjima T, Bauer G, Li H, Li MJ, Ehsani A, et al. Expression of small interfering RNAs targeted against HIV-1 rev transcripts in human cells. Nat Biotechnol 2002; 20:500-5.
6. Miyagishi M, Taira K. U6 promoter-driven siRNAs with four uridine 3′ overhangs efficiently suppress targeted gene expression in mammalian cells. Nat Biotechnol 2002; 20:497-500.
7. Paul CP, Good PD, Winer I, Engelke DR. Effective expression of small interfering RNA in human cells. Nat Biotechnol 2002; 20:505-8.
8. Golding MC, Long CR, Carmell MA, Hannon GJ, Westhusin ME. Suppression of prion protein in livestock by RNA interference. Proc Natl Acad Sci USA 2006; 103:5285-90.
9. Gallozzi M, Chapuis J, Le Provost F, Le Dur A, Morgenthaler C, Peyre C, et al. Prnp knockdown in transgenic mice using RNA interference. Transgenic Res 2008; 17:783-91.
10. Pfeifer A, Eigenbrod S, Al-Khadra S, Hofmann A, Mitteregger G, Moser M, et al. Lentivector-mediated RNAi efficiently suppresses prion protein and prolongs survival of scrapie-infected mice. J Clin Invest 2006; 116:3204-10.
11. White MD, Farmer M, Mirabile I, Brandner S, Collinge J, Mallucci GR. Single treatment with RNAi against prion protein rescues early neuronal dysfunction and prolongs survival in mice with prion disease. Proc Natl Acad Sci USA 2008; 105:10238-43.
12. Hasuwa H, Kaseda K, Einarsdottir T, Okabe M. Small interfering RNA and gene silencing in transgenic mice and rats. FEBS Lett 2002; 532:227-30.
13. Hiraoka-Kanie M, Miyagishi M, Yamashita JK. Differentiation stage-specific analysis of gene function with inducible short hair-pin RNA in differentiating embryonic stem cells. Biochem Biophys Res Commun 2006; 351:669-74.
14. Kawasaki H, Taira K. Short hairpin type of dsRNAs that are controlled by tRNA(Val) promoter significantly induce RNAi-mediated gene silencing in the cytoplasm of human cells. Nucleic Acids Res 2003; 31:700-7.
15. Gunnery S, Ma Y, Mathews MB. Termination sequence requirements vary among genes transcribed by RNA polymerase III. J Mol Biol 1999; 286:745-57.
16. Sullenger BA, Lee TC, Smith CA, Ungers GE, Gilboa E. Expression of chimeric tRNA-driven antisense transcripts renders NIH 3T3 cells highly resistant to Moloney murine leukemia virus replication. Mol Cell Biol 1990; 10:6512-23.
17. Yu M, Ojwang J, Yamada O, Hampel A, Rapapport J, Looney D, et al. A hairpin ribozyme inhibits expression of diverse strains of human immunodeficiency virus type 1. Proc Nat Acad Sci USA 1993; 90:6340-4.
18. Boden D, Pusch O, Lee F, Tucker L, Shank PR, Ramratnam B. Promoter choice affects the potency of HIV-1 specific RNA interference. Nucleic Acids Res 2003; 31:5033-8.
19. Makinen PI, Koponen JK, Karkkainen AM, Malm TM, Pulkkinen KH, Koistinaho J, et al. Stable RNA interference: comparison of U6 and H1 promoters in endothelial cells and in mouse brain. J Gene Med 2006; 8:433-41.
20. Grimm D, Streetz KL, Jopling CL, Storm TA, Pandey K, Davis CR, et al. Fatality in mice due to oversaturation of cellular microRNA/short hairpin RNA pathways. Nature 2006; 441:537-41.
21. Caplen NJ, Parrish S, Imani F, Fire A, Morgan RA. Specific inhibition of gene expression by small double-stranded RNAs in invertebrate and vertebrate systems. Proc Natl Acad Sci USA 2001; 98:9742-7.
22. Elbashir SM, Harborth J, Lendeckel W, Yalcin A, Weber K, Tuschl T. Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells. Nature 2001; 411:494-8.
23. Sutou S, Kunishi M, Kudo T, Wongsrikeao P, Miyagishi M, Otoi T. Knockdown of the bovine prion gene PRNP by RNA interference (RNAi) technology. BMC Biotechnol 2007; 7:44.
24. Brummelkamp TR, Bernards R, Agami R. A system for stable expression of short interfering RNAs in mammalian cells. Science 2002; 296:550-3.
25. Yi R, Doehle BP, Qin Y, Macara IG, Cullen BR. Overexpression of exportin 5 enhances RNA interference mediated by short hairpin RNAs and microRNAs. RNA 2005; 11:220-6.
26. Judge AD, Sood V, Shaw JR, Fang D, McClintock K, MacLachlan I. Sequence-dependent stimulation of the mammalian innate immune response by synthetic siRNA. Nat Biotechnol 2005; 23:457-62.
27. An DS, Qin FX, Auyeung VC, Mao SH, Kung SK, Baltimore D, et al. Optimization and functional effects of stable short hairpin RNA expression in primary human lymphocytes via lentiviral vectors. Mol Ther 2006; 14:494-504.
28. Ikawa M, Kominami K, Yoshimura Y, Tanaka K, Nishimune Y, Okabe M. A rapid and non-invasive selection of transgenic embryos before implantation using green fluorescent protein (GFP). FEBS Lett 1995; 375:125-8.
29. Young LE, Sinclair KD, Wilmut I. Large offspring syndrome in cattle and sheep. Rev Reprod 1998; 3:155-63. (Pubitemid 28508646)
30. Amor DJ, Halliday J. A review of known imprinting syndromes and their association with assisted reproduction technologies. Hum Reprod 2008; 23:2826-34.
31. Suzuki J Jr, Therrien J, Filion F, Lefebvre R, Goff AK, Smith LC. In vitro culture and somatic cell nuclear transfer affect imprinting of SNRPN gene in pre- and post-implantation stages of development in cattle. BMC Dev Biol 2009; 9:9.
32. Long JE, Cai X. Igf-2r expression regulated by epigenetic modification and the locus of gene imprinting disrupted in cloned cattle. Gene 2007; 388:125-34. (Pubitemid 46014021)
33. Zhang S, Kubota C, Yang L, Zhang Y, Page R, O'Neill M, et al. Genomic imprinting of H19 in naturally reproduced and cloned cattle. Biol Reprod 2004; 71:1540-4. (Pubitemid 39403248)
34. Curchoe CL, Zhang S, Yang L, Page R, Tian XC. Hypomethylation trends in the intergenic region of the imprinted IGF2 and H19 genes in cloned cattle. Anim Reprod Sci 2009; 116:213-25.
35. Liu JH, Yin S, Xiong B, Hou Y, Chen DY, Sun QY. Aberrant DNA methylation imprints in aborted bovine clones. Mol Reprod Dev 2008; 75:598-607. (Pubitemid 351323769)
36. Inoue Y. Life cycle of yeast prions: propagation mediated by amyloid fibrils. Protein Pept Lett 2009; 16:271-6.
37. Aguzzi A, Baumann F, Bremer J. The prion's elusive reason for being. Annu Rev Neurosci 2008; 31:439-77.
38. Sakudo A, Ikuta K. Fundamentals of prion diseases and their involvement in the loss of function of cellular prion protein. Protein Pept Lett 2009; 16:217-29.
39. Cobb NJ, Surewicz WK. Prion diseases and their biochemical mechanisms. Biochemistry 2009; 48:2574-85.
40. Bremer J, Baumann F, Tiberi C, Wessig C, Fischer H, Schwarz P, et al. Axonal prion protein is required for peripheral myelin maintenance. Nat Neurosci 13:310-8.
41. Bueler H, Aguzzi A, Sailer A, Greiner RA, Autenried P, Aguet M, et al. Mice devoid of PrP are resistant to scrapie. Cell 1993; 73:1339-47. (Pubitemid 23201147)
42. Mallucci G, Dickinson A, Linehan J, Klohn PC, Brandner S, Collinge J. Depleting neuronal PrP in prion infection prevents disease and reverses spongiosis. Science 2003; 302:871-4. (Pubitemid 37339628)
43. Bueler H, Fischer M, Lang Y, Bluethmann H, Lipp HP, DeArmond SJ, et al. Normal development and behaviour of mice lacking the neuronal cell-surface PrP protein. Nature 1992; 356:577-82.
44. Wongsrikeao P, Nagai T, Agung B, Taniguchi M, Kunishi M, Suto S, et al. Improvement of transgenic cloning efficiencies by culturing recipient oocytes and donor cells with antioxidant vitamins in cattle. Mol Reprod Dev 2007; 74:694-702. (Pubitemid 46676832)
45. Kwun J, Chang K, Lim J, Lee E, Lee B, Kang S, et al. Effects of exogenous hexoses on bovine in vitro fertilized and cloned embryo development: Improved blastocyst formation after glucose replacement with fructose in a serum-free culture medium. Mol Reprod Dev 2003; 65:167-74. (Pubitemid 36539206)
46. Suzuki T, Sumantri C, Khan NH, Murakami M, Saha S. Development of a simple, portable carbon dioxide incubator for in vitro production of bovine embryos. Anim Reprod Sci 1999; 54:149-57. (Pubitemid 29062497)