Feasibility for a large scale mouse mutagenesis by injecting CRISPR/Cas plasmid into zygotes

Development Growth and Differentiation

Volumn 56, Issue 1, 2014, Pages 122-129

  Mashiko, Daisuke ^a,b   Young, Samantha A M ^a,c   Muto, Masanaga ^a,d   Kato, Hirotaka ^a,d   Nozawa, Kaori ^a,b   Ogawa, Masaki ^a,d   Noda, Taichi ^a   Kim, Yeon Joo ^a   Satouh, Yuhkoh ^a   Fujihara, Yoshitaka ^a   Ikawa, Masahito ^a,b,d  

^a OSAKA UNIVERSITY   (Japan)

^b OSAKA UNIVERSITY GRADUATE SCHOOL OF MEDICINE   (Japan)

^c UNIVERSITY OF NEWCASTLE   (Australia)

^d OSAKA UNIVERSITY   (Japan)

Author keywords

Eucomm; International Knockout Mouse Consortium; International Mouse Phenotyping Consortium; Knockout; Knockout mouse project, KOMP

Indexed keywords

GREEN FLUORESCENT PROTEIN; GUIDE RNA; PLASMID DNA;

ALLELE; ANIMAL CELL; ARTICLE; AUTOSOME; COMPUTER PROGRAM; CONTROLLED STUDY; CRISPR CAS SYSTEM; DNA REPAIR; EMBRYO; FEASIBILITY STUDY; FEMALE; GENE ACTIVITY; GENE CASSETTE; GENETIC CODE; GENETIC TRANSFECTION; GERM LINE; HOMOLOGY DEPENDENT REPAIR; IN VITRO STUDY; MALE; MOUSE; MUTATION RATE; NONHUMAN; PLASMID; PROTEIN EXPRESSION; RNA SEQUENCE; SITE DIRECTED MUTAGENESIS; ZYGOTE; ANIMAL; GENE TARGETING; GENETICS; HEK293 CELL LINE; HUMAN; METABOLISM; MUTANT MOUSE STRAIN; MUTATION; PROCEDURES;

ANIMALS; CRISPR-CAS SYSTEMS; FEASIBILITY STUDIES; GENE TARGETING; HEK293 CELLS; HUMANS; MICE; MICE, MUTANT STRAINS; MUTAGENESIS, SITE-DIRECTED; MUTATION; PLASMIDS; ZYGOTE;

EID: 84892659943     PISSN: 00121592     EISSN: 1440169X     Source Type: Journal    
DOI: 10.1111/dgd.12113     Document Type: Article

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