The role of nanotechnology in induced pluripotent and embryonic stem cells research

Journal of Biomedical Nanotechnology

Volumn 10, Issue 12, 2014, Pages 3431-3461

  Chen, Lukui ^a   Qiu, Rong ^a   Li, Lushen ^b  

^a AFFILIATED ZHONGDA HOSPITAL OF SOUTHEAST UNIVERSITY   (China)

^b SOUTHEAST UNIVERSITY   (China)

Author keywords

Embryonic stem cells; Induced pluripotent stem cells; Nanomaterials; Nanotechnology

Indexed keywords

CELL ENGINEERING; CELLS; CYTOLOGY; DISEASE CONTROL; NANOSTRUCTURED MATERIALS; NANOTECHNOLOGY; PATIENT TREATMENT; TISSUE; TISSUE ENGINEERING;

EMBRYONIC STEM CELLS; ETHICAL PROBLEMS; EXTRACELLULAR MATRICES; INDUCED PLURIPOTENT STEM CELLS; PROMISING THERAPY; QUALITY OF LIFE; REGENERATIVE MEDICINE; STEM CELL THERAPY;

STEM CELLS;

CELL DIFFERENTIATION; CELL TRACKING; EMBRYONIC STEM CELL; GENETIC TRANSFECTION; HUMAN; NANOTECHNOLOGY; NONHUMAN; PLURIPOTENT STEM CELL; REGENERATIVE MEDICINE; REVIEW; STEM CELL RESEARCH; TISSUE ENGINEERING; ANIMAL; CHEMISTRY; CYTOLOGY; STEM CELL TRANSPLANTATION; TRANSPLANTATION; TRENDS;

NANOPARTICLE;

ANIMALS; EMBRYONIC STEM CELLS; HUMANS; NANOPARTICLES; NANOTECHNOLOGY; PLURIPOTENT STEM CELLS; STEM CELL RESEARCH; STEM CELL TRANSPLANTATION; TISSUE ENGINEERING;

EID: 84929666738     PISSN: 15507033     EISSN: 15507041     Source Type: Journal    
DOI: 10.1166/jbn.2014.2043     Document Type: Review

References (297)

1. L. Wang, Y. Huang, Q. Guo, X. Fan, Y. Lu, S. Zhu, Y. Wang, X. Bo, X. Chang, M. Zhu, and Z. Wang, Differentiation of iPSCs into insulin-producing cells via adenoviral transfection of PDX-1, NeuroD1 and MafA. Diabetes Res. Clin. Pract. 104, 383 (2014).
2. A. Nsair and W. R. MacLellan, Induced pluripotent stem cells for regenerative cardiovascular therapies and biomedical discovery. Adv. Drug Deliv. Rev. 63, 324 (2011).
3. J. Lock and H. Liu, Nanomaterials enhance osteogenic differentiation of human mesenchymal stem cells similar to a short peptide of BMP-7. Int. J. Nanomedicine 6, 2769 (2011).
4. A. E. Nel, L. Madler, D. Velegol, T. Xia, E. M. Hoek, P. Somasundaran, F. Klaessig, V. Castranova, and M. Thompson, Understanding biophysicochemical interactions at the nano-bio interface. Nat. Mater. 8, 543 (2009).
5. A. C. Wan and J. Y. Ying, Nanomaterials for in situ cell delivery and tissue regeneration. Adv. Drug Deliv. Rev. 62, 731 (2010).
6. A. Orza, O. Soritau, L. Olenic, M. Diudea, A. Florea, D. Rus Ciuca, C. Mihu, D. Casciano, and A. S. Biris, Electrically conductive goldcoated collagen nanofibers for placental-derived mesenchymal stem cells enhanced differentiation and proliferation. ACS Nano 5, 4490 (2011).
7. I. Wimpenny, H. Markides, and A. J. El Haj, Orthopaedic applications of nanoparticle-based stem cell therapies. Stem Cell Res. Ther. 3, 13 (2012).
8. A. Ao, J. Hao, and C. C. Hong, Regenerative chemical biology: Current challenges and future potential. Chem. Biol. 18, 413 (2011).
9. S. Oh, K. S. Brammer, Y. S. Li, D. Teng, A. J. Engler, S. Chien, and S. Jin, Stem cell fate dictated solely by altered nanotube dimension. Proc. Natl. Acad. Sci. U.S.A. 106, 2130 (2009).
10. I. O. Smith, X. H. Liu, L. A. Smith, and P. X. Ma, Nanostructured polymer scaffolds for tissue engineering and regenerative medicine. Rev. Nanomed. Nanobiotechnol. 1, 226 (2009).
11. E. Dawson, G. Mapili, K. Erickson, S. Taqvi, and K. Roy, Biomaterials for stem cell differentiation. Adv. Drug Deliv. Rev. 60, 215
12. N. S. Hwang, S. Varghese, and J. Elisseeff, Controlled differentiation of stem cells. Adv. Drug Deliv. Rev. 60, 199 (2008).
13. F. Grinnell and M. H. Bennett, Ultrastructural studies of cell-collagen interactions. Methods Enzymol. 82 Pt A, 535 (1982).
14. A. Domogatskaya, S. Rodin, A. Boutaud, and K. Tryggvason, Laminin-511 but not -332, -111, or -411 enables mouse embryonic stem cell self-renewal in vitro. Stem Cells 26, 2800 (2008).
15. S. Li, D. Edgar, R. Fassler, W. Wadsworth, and P. D. Yurchenco, The role of laminin in embryonic cell polarization and tissue organization. Dev. Cell 4, 613 (2003).
16. M. Amit, C. Shariki, V. Margulets, and J. Itskovitz-Eldor, Feeder layer- and serum-free culture of human embryonic stem cells. Biol. Reprod. 70, 837 (2004).
17. C. Xu, M. S. Inokuma, J. Denham, K. Golds, P. Kundu, J. D. Gold, and M. K. Carpenter, Feeder-free growth of undifferentiated human embryonic stem cells. Nat. Biotechnol. 19, 971 (2001).
18. D. Philp, S. S. Chen, W. Fitzgerald, J. Orenstein, L. Margolis, and H. K. Kleinman, Complex extracellular matrices promote tissuespecific stem cell differentiation. Stem Cells 23, 288 (2005).
19. R. O. Hynes, Integrins: Bidirectional, allosteric signaling machines. Cell 110, 673 (2002).
20. E. H. Danen and K. M. Yamada, Fibronectin, integrins, and growth control. J. Cell. Physiol. 189, 1 (2001).
21. F. Ramirez and D. B. Rifkin, Cell signaling events: A view from the matrix. Matrix Biol. 22, 101 (2003).
22. S. Li, D. Harrison, S. Carbonetto, R. Fässler, N. Smyth, D. Edgar, and P. D. Yurchenco, Matrix assembly, regulation, and survival functions of laminin and its receptors in embryonic stem cell differentiation. J. Cell Biol. 157, 1279 (2002).
23. H. J. Lin, T. J. O'Shaughnessy, J. Kelly, and W. Ma, Neural stem cell differentiation in a cell-collagen-bioreactor culture system. Brain Res. Dev. Brain Res. 153, 163 (2004).
24. W. Ma, T. Tavakoli, E. Derby, Y. Serebryakova, M. S. Rao, and M. P. Mattson, Cell-extracellular matrix interactions regulate neural differentiation of human embryonic stem cells. BMC Dev. Biol. 8, 90 (2008).
25. L. Ji, V. L. LaPointe, N. D. Evans, and M. M. Stevens, Changes in embryonic stem cell colony morphology and early differentiation markers driven by colloidal crystal topographical cues. Eur. Cell Mater. 23, 135 (2012).
26. E. K. A. Nur, I. Ahmed, J. Kamal, M. Schindler, and S. Meiners, Three-dimensional nanofibrillar surfaces promote self-renewal in mouse embryonic stem cells. Stem Cells 24, 426 (2006).
27. E. Kingham and M. Welham, Distinct roles for isoforms of the catalytic subunit of class-IA PI3K in the regulation of behaviour of murine embryonic stem cells. J. Cell Sci. 122, 2311 (2009).
28. N. R. Paling, H. Wheadon, H. K. Bone, and M. J. Welham, Regulation of embryonic stem cell self-renewal by phosphoinositide 3-kinase-dependent signaling. J. Biol. Chem. 279, 48063 (2004).
29. M. P. Storm, H. K. Bone, C. G. Beck, P. Y. Bourillot, V. Schreiber, T. Damiano, A. Nelson, P. Savatier, and M. J. Welham, Regulation of Nanog expression by phosphoinositide 3-kinase-dependent signaling in murine embryonic stem cells. J. Biol. Chem. 282, 6265 (2007).
30. H. Sun, R. Lesche, D. M. Li, J. Liliental, H. Zhang, J. Gao, N. Gavrilova, B. Mueller, X. Liu, and H. Wu, PTEN modulates cell cycle progression and cell survival by regulating phosphatidylinositol 3,4,5,-trisphosphate and Akt/protein kinase B signaling pathway. Proc. Natl. Acad. Sci. U.S.A. 96, 6199 (1999).
31. L. Jirmanova, M. Afanassieff, S. Gobert-Gosse, S. Markossian, and P. Savatier, Differential contributions of ERK and PI3-kinase to the regulation of cyclin D1 expression and to the control of the G1/S transition in mouse embryonic stem cells. Oncogene 21, 5515 (2002).
32. E. Freire, F. C. Gomes, R. Linden, V. M. Neto, and T. Coelho-Sampaio, Structure of laminin substrate modulates cellular signaling for neuritogenesis. J. Cell Sci. 115, 4867 (2002).
33. W. He and R. V. Bellamkonda, Nanoscale neuro-integrative coatings for neural implants. Biomaterials 26, 2983 (2005).
34. E. Jan and N. A. Kotov, Successful differentiation of mouse neural stem cells on layer-by-layer assembled single-walled carbon nanotube composite. Nano Lett. 7, 1123 (2007).
35. Y. Ni, H. Hu, E. B. Malarkey, B. Zhao, V. Montana, R. C. Haddon, and V. Parpura, Chemically functionalized water soluble singlewalled carbon nanotubes modulate neurite outgrowth. J. Nanosci. Nanotechnol. 5, 1707 (2005).
36. I. Sridharan, T. Kim, and R. Wang, Adapting collagen/CNT matrix in directing hESC differentiation. Biochem. Biophys. Res. Commun. 381, 508 (2009).
37. B. Li, Y. Ma, S. Wang, and P. M. Moran, Influence of carboxyl group density on neuron cell attachment and differentiation behavior: Gradient-guided neurite outgrowth. Biomaterials 26, 4956 (2005).
38. M. E. Levenstein, T. E. Ludwig, R. H. Xu, R. A. Llanas, K. VanDenHeuvel-Kramer, D. Manning, and J. A. Thomson, Basic fibroblast growth factor support of human embryonic stem cell selfrenewal. Stem Cells 24, 568 (2006).
39. B. Li, Y. Ma, S. Wang, and P. M. Moran, Influence of carboxyl group density on neuron cell attachment and differentiation behavior: Gradient-guided neurite outgrowth. Biomaterials 26, 4956 (2005).
40. I. Sridharan, T. Kim, and R. Wang, Adapting collagen/CNT matrix in directing hESC differentiation. Biochem. Biophys. Res. Commun. 381, 508 (2009).
41. S. M. Dang, S. Gerecht-Nir, J. Chen, J. Itskovitz-Eldor, and P. W. Zandstra, Controlled, scalable embryonic stem cell differentiation culture. Stem Cells 22, 275 (2004).
42. J. A. Thomson, J. Itskovitz-Eldor, S. S. Shapiro, M. A. Waknitz, J. J. Swiergiel, V. S. Marshall, and J. M. Jones, Embryonic stem cell lines derived from human blastocysts. Science 282, 1145 (1998).
43. G. Y. Chen, D. W. Pang, S. M. Hwang, H. Y. Tuan, and Y. C. Hu, A graphene-based platform for induced pluripotent stem cells culture and differentiation. Biomaterials 33, 418 (2012).
44. L. Wang, L. Li, P. Menendez, C. Cerdan, and M. Bhatia, Human embryonic stem cells maintained in the absence of mouse embryonic fibroblasts or conditioned media are capable of hematopoietic development. Blood 105, 4598 (2005).
45. E. K. A. Nur, I. Ahmed, J. Kamal, A. N. Babu, M. Schindler, and S. Meiners, Covalently attached FGF-2 to three-dimensional polyamide nanofibrillar surfaces demonstrates enhanced biological stability and activity. Mol. Cell Biochem. 309, 157 (2008).
46. M. J. Mondrinos, S. Koutzaki, E. Jiwanmall, M. Li, J. P. Dechadarevian, P. I. Lelkes, and C. M. Finck, Engineering threedimensional pulmonary tissue constructs. Tissue Eng. 12, 717 (2006).
47. C. S. Young, H. Abukawa, R. Asrican, M. Ravens, M. J. Troulis, L. B. Kaban, J. P. Vacanti, and P. C. Yelick, Tissue-engineered hybrid tooth and bone. Tissue Eng. 11, 1599 (2005).
48. X. Kang, Y. Xie, H. M. Powell, L. James Lee, M. A. Belury, J. J. Lannutti, and D. A. Kniss, Adipogenesis of murine embryonic stem cells in a three-dimensional culture system using electrospun polymer scaffolds. Biomaterials 28, 450 (2007).
49. S. Levenberg, N. F. Huang, E. Lavik, A. B. Rogers, J. Itskovitz-Eldor, and R. Langer, Differentiation of human embryonic stem cells on three-dimensional polymer scaffolds. Proc. Natl. Acad. Sci. U.S.A. 100, 12741 (2003).
50. D. J. Mooney and H. Vandenburgh, Cell delivery mechanisms for tissue repair. Cell Stem Cell 2, 205 (2008).
51. G. A. Silva, C. Czeisler, K. L. Niece, E. Beniash, D. A. Harrington, J. A. Kessler, and S. I. Stupp, Selective differentiation of neural progenitor cells by high-epitope density nanofibers. Science 303, 1352 (2004).
52. S. M. Willerth and S. E. Sakiyama-Elbert, Approaches to neural tissue engineering using scaffolds for drug delivery. Adv. Drug Deliv. Rev. 59, 325 (2007).
53. H. Hakala, K. Rajala, M. Ojala, S. Panula, S. Areva, M. Kellomaki, R. Suuronen, and H. Skottman, Comparison of biomaterials and extracellular matrices as a culture platform for multiple, independently derived human embryonic stem cell lines. Tissue Eng. Part A 15, 1775 (2009).
54. L. G. Villa-Diaz, H. Nandivada, J. Ding, N. C. Nogueira-de-Souza, P. H. Krebsbach, K. S. O'Shea, J. Lahann, and G. D. Smith, Synthetic polymer coatings for long-term growth of human embryonic stem cells. Nat. Biotechnol. 28, 581 (2010).
55. J. R. Klim, L. Li, P. J. Wrighton, M. S. Piekarczyk, and L. L. Kiessling, A defined glycosaminoglycan-binding substratum for human pluripotent stem cells. Nat. Methods 7, 989 (2010).
56. Z. Melkoumian, J. L. Weber, D. M. Weber, A. G. Fadeev, Y. Zhou, P. Dolley-Sonneville, J. Yang, L. Qiu, C. A. Priest, C. Shogbon, A. W. Martin, J. Nelson, P. West, J. P. Beltzer, S. Pal, and R. Brandenberger, Synthetic peptide-acrylate surfaces for long-term self-renewal and cardiomyocyte differentiation of human embryonic stem cells. Nat. Biotechnol. 28, 606 (2010).
57. W. Chen, L. G. Villa-Diaz, Y. Sun, S. Weng, J. K. Kim, R. H. Lam, L. Han, R. Fan, P. H. Krebsbach, and J. Fu, Nanotopography influences adhesion, spreading, and self-renewal of human embryonic stem cells. ACS Nano 6, 4094 (2012).
58. J. Itskovitz-Eldor, M. Schuldiner, D. Karsenti, A. Eden, O. Yanuka, M. Amit, H. Soreq, and N. Benvenisty, Differentiation of human embryonic stem cells into embryoid bodies compromising the three embryonic germ layers. Mol. Med. 6, 88 (2000).
59. S. Battista, D. Guarnieri, C. Borselli, S. Zeppetelli, A. Borzacchiello, L. Mayol, D. Gerbasio, D. R. Keene, L. Ambrosio, and P. A. Netti, The effect of matrix composition of 3D constructs on embryonic stem cell differentiation. Biomaterials 26, 6194 (2005).
60. S. K. Dhara and S. L. Stice, Neural differentiation of human embryonic stem cells. J. Cell Biochem. 105, 633 (2008).
61. J. Xie, S. M. Willerth, X. Li, M. R. Macewan, A. Rader, S. E. Sakiyama-Elbert, and Y. Xia, The differentiation of embryonic stem cells seeded on electrospun nanofibers into neural lineages. Biomaterials 30, 354 (2009).
62. F. Yang, R. Murugan, S. Wang, and S. Ramakrishna, Electrospinning of nano/micro scale poly(L-lactic acid) aligned fibers and their potential in neural tissue engineering. Biomaterials 26, 2603 (2005).
63. G. T. Christopherson, H. Song, and H. Q. Mao, The influence of fiber diameter of electrospun substrates on neural stem cell differentiation and proliferation. Biomaterials 30, 556 (2009).
64. R. G. Ellis-Behnke, Y. X. Liang, S. W. You, D. K. Tay, S. Zhang, K. F. So, and G. E. Schneider, Nano neuro knitting: Peptide nanofiber scaffold for brain repair and axon regeneration with functional return of vision. Proc. Natl. Acad. Sci. U.S.A. 103, 5054 (2006).
65. E. K. Yim, S. W. Pang, and K. W. Leong, Synthetic nanostructures inducing differentiation of human mesenchymal stem cells into neuronal lineage. Exp. Cell Res. 313, 1820 (2007).
66. Y. R. Shih, C. N. Chen, S. W. Tsai, Y. J. Wang, and O. K. Lee, Growth of mesenchymal stem cells on electrospun type I collagen nanofibers. Stem Cells 24, 2391 (2006).
67. S. J. Kim, J. K. Lee, J. W. Kim, J. W. Jung, K. Seo, S. B. Park, K. H. Roh, S. R. Lee, Y. H. Hong, S. J. Kim, Y. S. Lee, S. J. Kim, and K. S. Kang, Surface modification of polydimethylsiloxane (PDMS) induced proliferation and neural-like cells differentiation of umbilical cord blood-derived mesenchymal stem cells. J. Mater. Sci. Mater. Med. 19, 2953 (2008).
68. J. B. Recknor, D. S. Sakaguchi, and S. K. Mallapragada, Directed growth and selective differentiation of neural progenitor cells on micropatterned polymer substrates. Biomaterials 27, 4098 (2006).
69. L. A. Smith, X. Liu, J. Hu, and P. X. Ma, The influence of threedimensional nanofibrous scaffolds on the osteogenic differentiation of embryonic stem cells. Biomaterials 30, 2516 (2009).
70. L. A. Smith, X. Liu, J. Hu, P. Wang, and P. X. Ma, Enhancing osteogenic differentiation of mouse embryonic stem cells by nanofibers. Tissue Eng. Part A 15, 1855 (2009).
71. M. E. Davis, J. P. Motion, D. A. Narmoneva, T. Takahashi, D. Hakuno, R. D. Kamm, S. Zhang, and R. T. Lee, Injectable self-assembling peptide nanofibers create intramyocardial microenvironments for endothelial cells. Circulation 111, 442 (2005).
72. K. Kurpinski, J. Chu, C. Hashi, and S. Li, Anisotropic mechanosensing by mesenchymal stem cells. Proc. Natl. Acad. Sci. U.S.A. 103, 16095 (2006).
73. E. D. O'Cearbhaill, M. A. Punchard, M. Murphy, F. P. Barry, P. E. McHugh, and V. Barron, Response of mesenchymal stem cells to the biomechanical environment of the endothelium on a flexible tubular silicone substrate. Biomaterials 29, 1610 (2008).
74. S. A. Ruiz and C. S. Chen, Emergence of patterned stem cell differentiation within multicellular structures. Stem Cells 26, 2921 (2008).
75. R. McBeath, D. M. Pirone, C. M. Nelson, K. Bhadriraju, and C. S. Chen, Cell shape, cytoskeletal tension, and RhoA regulate stem cell lineage commitment. Dev. Cell 6, 483 (2004).
76. J. Park, C. H. Cho, N. Parashurama, Y. Li, F. Berthiaume, M. Toner, A. W. Tilles, and M. L. Yarmush, Microfabrication-based modulation of embryonic stem cell differentiation. Lab. Chip 7, 1018 (2007).
77. A. J. Engler, S. Sen, H. L. Sweeney, and D. E. Discher, Matrix elasticity directs stem cell lineage specification. Cell 126, 677 (2006).
78. C. K. Hashi, Y. Zhu, G. Y. Yang, W. L. Young, B. S. Hsiao, K. Wang, B. Chu, and S. Li, Antithrombogenic property of bone marrow mesenchymal stem cells in nanofibrous vascular grafts. Proc. Natl. Acad. Sci. U.S.A. 104, 11915 (2007).
79. X. Xin, M. Hussain, and J. J. Mao, Continuing differentiation of human mesenchymal stem cells and induced chondrogenic and osteogenic lineages in electrospun PLGA nanofiber scaffold. Biomaterials 28, 316 (2007).
80. M. J. Dalby, N. Gadegaard, R. Tare, A. Andar, M. O. Riehle, P. Herzyk, C. D. Wilkinson, and R. O. Oreffo, The control of human mesenchymal cell differentiation using nanoscale symmetry and disorder. Nat. Mater. 6, 997 (2007).
81. S. Oh, K. S. Brammer, Y. S. Li, D. Teng, A. J. Engler, S. Chien, and S. Jin, Stem cell fate dictated solely by altered nanotube dimension. Proc. Natl. Acad. Sci. U.S.A. 106, 2130 (2009).
82. A. Pietroiusti, M. Massimiani, I. Fenoglio, M. Colonna, F. Valentini, G. Palleschi, A. Camaioni, A. Magrini, G. Siracusa, A. Bergamaschi, A. Sgambato, and L. Campagnolo, Low doses of pristine and oxidized single-wall carbon nanotubes affect mammalian embryonic development. ACS Nano 5, 4624 (2011).
83. W. Deng, Induced pluripotent stem cells: Paths to new medicines. A catalyst for disease modelling, drug discovery and regenerative therapy. EMBO Rep. 11, 161 (2010).
84. R. Madonna, Human-induced pluripotent stem cells: In quest of clinical applications. Mol. Biotechnol. 52, 193 (2012).
85. T. Dvir, B. P. Timko, D. S. Kohane, and R. Langer, Nanotechnological strategies for engineering complex tissues. Nat. Nanotechnol. 6, 13 (2011).
86. D. H. Kim, E. A. Lipke, P. Kim, R. Cheong, S. Thompson, M. Delannoy, K. Y. Suh, L. Tung, and A. Levchenko, Nanoscale cues regulate the structure and function of macroscopic cardiac tissue constructs. Proc. Natl. Acad. Sci. U.S.A. 107, 565 (2010).
87. H. Hosseinkhani, M. Hosseinkhani, N. P. Gabrielson, D. W. Pack, A. Khademhosseini, and H. Kobayashi, DNA nanoparticles encapsulated in 3D tissue-engineered scaffolds enhance osteogenic differentiation of mesenchymal stem cells. J. Biomed. Mater. Res. A
88. X. Yang, X. F. Walboomers, J. van den Dolder, F. Yang, Z. Bian, M. Fan, and J. A. Jansen, Non-viral bone morphogenetic protein 2 transfection of rat dental pulp stem cells using calcium phosphate nanoparticles as carriers. Tissue Eng. Part A 14, 71 (2008).
89. J. J. Green, B. Y. Zhou, M. M. Mitalipova, C. Beard, R. Langer, R. Jaenisch, and D. G. Anderson, Nanoparticles for gene transfer to human embryonic stem cell colonies. Nano Lett. 8, 3126 (2008).
90. K. Kutsuzawa, T. Akaike, and E. H. Chowdhury, The influence of the cell-adhesive proteins E-cadherin and fibronectin embedded in carbonate-apatite DNA carrier on transgene delivery and expression in a mouse embryonic stem cell line. Biomaterials 29, 370 (2008).
91. K. Kutsuzawa, K. Maruyama, Y. Akiyama, T. Akaike, and E. H. Chowdhury, Efficient transfection of mouse embryonic stem cells with cell-adhesive protein-embedded inorganic nanocarrier. Anal. Biochem. 372, 122 (2008).
92. C. H. Lee, J. H. Kim, H. J. Lee, K. Jeon, H. Lim, Hy. Choi, E. R. Lee, S. H. Park, J. Y. Park, S. Hong, S. Kim, and S. G. Cho, The generation of iPS cells using non-viral magnetic nanoparticle based transfection. Biomaterials 32, 6683 (2011).
93. J. Ruan, J. Shen, Z. Wang, J. Ji, H. Song, K. Wang, B. Liu, J. Li, and D. Cui, Efficient preparation and labeling of human induced pluripotent stem cells by nanotechnology. Int. J. Nanomedicine 6, 425 (2011).
94. R. Eiges, M. Schuldiner, M. Drukker, O. Yanuka, J. Itskovitz-Eldor, and N. Benvenisty, Establishment of human embryonic stem celltransfected clones carrying a marker for undifferentiated cells. Curr. Biol. 11, 514 (2001).
95. K. Kutsuzawa, E. H. Chowdhury, M. Nagaoka, K. Maruyama, Y. Akiyama, and T. Akaike, Surface functionalization of inorganic nano-crystals with fibronectin and E-cadherin chimera synergistically accelerates trans-gene delivery into embryonic stem cells. Biochem. Biophys. Res. Commun. 350, 514 (2006).
96. S. A. Anderson, J. Glod, A. S. Arbab, M. Noel, P. Ashari, H. A. Fine, and J. A. Frank, Noninvasive MR imaging of magnetically labeled stem cells to directly identify neovasculature in a glioma model. Blood 105, 420 (2005).
97. A. Iwanami, S. Kaneko, M. Nakamura, Y. Kanemura, H. Mori, S. Kobayashi, M. Yamasaki, S. Momoshima, H. Ishii, K. Ando, Y. Tanioka, N. Tamaoki, T. Nomura, Y. Toyama, and H. Okano, Transplantation of human neural stem cells for spinal cord injury in primates. J. Neurosci. Res. 80, 182 (2005).
98. P. K. Nguyen, J. Riegler, and J. C. Wu, Stem cell imaging: From bench to bedside. Cell Stem Cell 14, 431 (2014).
99. L. M. Ricles, S. Y. Nam, K. Sokolov, S. Y. Emelianov, and L. J. Suggs, Function of mesenchymal stem cells following loading of gold nanotracers. Int. J. Nanomedicine 6, 407 (2011).
100. S. M. Cromer Berman, P. Walczak, and J. W. Bulte, Tracking stem cells using magnetic nanoparticles. Wiley Interdiscip. Rev. Nanomed. Nanobiotechnol. 3, 343 (2011).
101. A. Bhirde, J. Xie, M. Swierczewska, and X. Chen, Nanoparticles for cell labeling. Nanoscale 3, 142 (2011).
102. A. O. El-Sadik, A. El-Ansary, and S. M. Sabry, Nanoparticlelabeled stem cells: A novel therapeutic vehicle. Clin. Pharmacol. 2, 9 (2010).
103. T. Arai, T. Kofidis, J. W. Bulte, J. de Bruin, R. D. Venook, G. J. Berry, M. V. Mcconnell, T. Quertermous, R. C. Robbins, and P. C. Yang, Dual in vivo magnetic resonance evaluation of magnetically labeled mouse embryonic stem cells and cardiac function at 1.5 t. Magn. Reson. Med. 55, 203 (2006).
104. K. W. Au, S. Y. Liao, Y. K. Lee, W. H. Lai, K. M. Ng, Y. C. Chan, M. C. Yip, C. Y. Ho, E. X. Wu, R. A. Li, C. W. Siu, and H. F. Tse, Effects of iron oxide nanoparticles on cardiac differentiation of embryonic stem cells. Biochem. Biophys. Res. Commun. 379, 898 (2009).
105. U. Himmelreich and M. Hoehn, Stem cell labeling for magnetic resonance imaging. Minim. Invasive Ther. Allied Technol. 17, 132 (2008).
106. M. Babic, D. Horák, M. Trchová, P. Jendelová, K. Glogarová, P. Lesný, V. Herynek, M. Hájek, and E. Syková, Poly(L-lysine)-modified iron oxide nanoparticles for stem cell labeling. Bioconjug. Chem. 19, 740 (2008).
107. D. Sponarová, D. Horák, M. Trchová, P. Jendelová, V. Herynek, N. Mitina, A. Zaichenko, R. Stoika, P. Lesný, and E. Syková, The use of oligoperoxide-coated magnetic nanoparticles to label stem cells. J. Biomed. Nanotechnol. 7, 384 (2011).
108. A. M. Reddy, B. K. Kwak, H. J. Shim, C. Ahn, S. H. Cho, B. J. Kim, S. Y. Jeong, S. J. Hwang, and S. H. Yuk, Functional characterization of mesenchymal stem cells labeled with a novel PVP-coated superparamagnetic iron oxide. Contrast Media Mol. Imaging 4, 118 (2009).
109. H. M. Liu, S. H. Wu, C. W. Lu, M. Yao, J. K. Hsiao, Y. Hung, Y. S. Lin, C. Y. Mou, C. S. Yang, D. M. Huang, and Y. C. Chen, Mesoporous silica nanoparticles improve magnetic labeling efficiency in human stem cells. Small 4, 619 (2008).
110. N. Muja and J. W. Bulte, Magnetic resonance imaging of cells in experimental disease models. Prog. Nucl. Magn. Reson. Spectrosc. 55, 61 (2009).
111. C. M. Long and J. W. Bulte, In vivo tracking of cellular therapeutics using magnetic resonance imaging. Expert Opin. Biol. Ther. 9, 293 (2009).
112. H. Lawall, P. Bramlage, and B. Amann, Stem cell and progenitor cell therapy in peripheral artery disease. A critical appraisal. Thromb. Haemost. 103, 696 (2010).
113. Y.Loh, Y. Oyama, L. Statkute, A. Traynor, J. Satkus, K. Quigley, K. Yaung, W. Barr, J. Bucha, M. Gheorghiade, and R. K. Burt, Autologous hematopoietic stem cell transplantation in systemic lupus erythematosus patients with cardiac dysfunction: Feasibility and reversibility of ventricular and valvular dysfunction with transplant-induced remission. Bone Marrow Transplant. 40, 47 (2007).
114. V. Mani, E. Adler, K. C. Briley-Saebo, A. Bystrup, V. Fuster, G. Keller, and Z. A. Fayad, Serial in vivo positive contrast MRI of iron oxide-labeled embryonic stem cell-derived cardiac precursor cells in a mouse model of myocardial infarction. Magn. Reson. Med. 60, 73 (2008).
115. A. Chaudeurge, C. Wilhelm, A. Chen-Tournoux, P. Farahmand, V. Bellamy, G. Autret, C. Menager, A. Hagege, J. Larghero, F. Gazeau, O. Clément, and P. Menasché, Can magnetic targeting of magnetically labeled circulating cells optimize intramyocardial cell retention? Cell Transplant. 21, 679 (2012).
116. C. Song, J. Xiang, J. Tang, D. G. Hirst, J. Zhou, K. M. Chan, and G. Li, Thymidine kinase gene modified bone marrow mesenchymal stem cells as vehicles for antitumor therapy. Hum. Gene Ther. 22, 439 (2010).
117. A. Yanai, U. O. Hafeli, A. L. Metcalfe, P. Soema, L. Addo, C. Y. Gregory-Evans, K. Po, X. Shan, O. L. Moritz, and K. Gregory-Evans, Focused magnetic stem cell targeting to the retina using superparamagnetic iron oxide nanoparticles. Cell Transplant. 21, 1137 (2012).
118. Y. Amsalem, Y. Mardor, M. S. Feinberg, N. Landa, L. Miller, D. Daniels, A. Ocherashvilli, R. Holbova, O. Yosef, I. M. Barbash, and J. Leor, Iron-oxide labeling and outcome of transplanted mesenchymal stem cells in the infarcted myocardium. Circulation 116, I38 (2007).
119. T. Arai, T. Kofidis, J. W. Bulte, J. de Bruin, R. D. Venook, G. J. Berry, M. V. McConnell, T. Quertermous, R. C. Robbins, and P. C. Yang, Dual in vivo magnetic resonance evaluation of magnetically labeled mouse embryonic stem cells and cardiac function at 1.5 t. Magn. Reson. Med. 55, 203 (2006).
120. D. L. Kraitchman, A. W. Heldman, E. Atalar, L. C. Amado, B. J. Martin, M. F. Pittenger, J. M. Hare, and J. W. Bulte, In vivo magnetic resonance imaging of mesenchymal stem cells in myocardial infarction. Circulation 107, 2290 (2003).
121. L. C. Amado, A. P. Saliaris, K. H. Schuleri, M. St John, J. S. Xie, S. Cattaneo, D. J. Durand, T. Fitton, J. Q. Kuang, G. Stewart, S. Lehrke, W. W. Baumgartner, B. J. Martin, A. W. Heldman, and J. M. Hare, Cardiac repair with intramyocardial injection of allogeneic mesenchymal stem cells after myocardial infarction. Proc. Natl. Acad. Sci. U.S.A. 102, 11474 (2005).
122. K. W. Au, S. Y. Liao, Y. K. Lee, W. H. Lai, K. M. Ng, Y. C. Chan, M. C. Yip, C. Y. Ho, E. X. Wu, R. A. Li, C. W. Siu, and H. F. Tse, Effects of iron oxide nanoparticles on cardiac differentiation of embryonic stem cells. Biochem. Biophys. Res. Commun. 379, 898 (2009).
123. E. Küstermann, W. Roell, M. Breitbach, S. Wecker, D. Wiedermann, C. Buehrle, A. Welz, J. Hescheler, B. K. Fleischmann, and M. Hoehn, Stem cell implantation in ischemic mouse heart: A high-resolution magnetic resonance imaging investigation. NMR Biomed. 18, 362 (2005).
124. M. Hoehn, E. Küstermann, J. Blunk, D. Wiedermann, T. Trapp, S. Wecker, M. Föcking, H. Arnold, J. Hescheler, B. K. Fleischmann, W. Schwindt, and C. Bührle, Monitoring of implanted stem cell migration in vivo: A highly resolved in vivo magnetic resonance imaging investigation of experimental stroke in rat. Proc. Natl. Acad. Sci. U.S.A. 99, 16267 (2002).
125. S. Lin, X. Xie, M. R. Patel, Y. H. Yang, Z. Li, F. Cao, O. Gheysens, Y. Zhang, S. S. Gambhir, J. H. Rao, and J. C. Wu, Quantum dot imaging for embryonic stem cells. BMC Biotechnol. 7, 67 (2007).
126. D. Nagesha, G. S. Laevsky, P. Lampton, R. Banyal, C. Warner, C. DiMarzio, and S. Sridhar, In vitro imaging of embryonic stem cells using multiphoton luminescence of gold nanoparticles. Int. J. Nanomedicine 2, 813 (2007).
127. M. B. Zaman, T. N. Baral, Z. J. Jakubek, J. Zhang, X. Wu, E. Lai, D. Whitfield, and K. Yu, Single-domain antibody bioconjugated near-IR quantum dots for targeted cellular imaging of pancreatic cancer. J. Nanosci. Nanotechnol. 11, 3757 (2011).
128. K. Yang, C. Zhao, Y. A. Cao, H. Tang, Y. L. Bai, H. Huang, C. R. Zhao, R. Chen, and D. Zhao, In vivo and in situ imaging of head and neck squamous cell carcinoma using near-infrared fluorescent quantum dot probes conjugated with epidermal growth factor receptor monoclonal antibodies in mice. Oncol. Rep. 27, 1925 (2012).
129. T. Sugiyama, S. Kuroda, T. Osanai, H. Shichinohe, Y. Kuge, M. Ito, M. Kawabori, and Y. Iwasaki, Near-infrared fluorescence labeling allows noninvasive tracking of bone marrow stromal cells transplanted into rat infarct brain. Neurosurgery 68, 1036 (2011).
130. M. Kawabori, S. Kuroda, T. Sugiyama, M. Ito, H. Shichinohe, K. Houkin, Y. Kuge, and N. Tamaki, Intracerebral, but not intravenous, transplantation of bone marrow stromal cells enhances functional recovery in rat cerebral infarct: An optical imaging study. Neuropathology 32, 217 (2012).
131. H. Yukawa, M. Watanabe, N. Kaji, Y. Okamoto, M. Tokeshi, Y. Miyamoto, H. Noguchi, Y. Baba, and S. Hayashi, Monitoring transplanted adipose tissue-derived stem cells combined with heparin in the liver by fluorescence imaging using quantum dots. Biomaterials 33, 2177 (2012).
132. A. Rak-Raszewska, M. Marcello, S. Kenny, D. Edgar, V. See, and P. Murray, Quantum dots do not affect the behaviour of mouse embryonic stem cells and kidney stem cells and are suitable for short-term tracking. PLoS One 7, e32650 (2012).
133. Y. Ramot, M. Steiner, V. Morad, S. Leibovitch, N. Amouyal, M. F. Cesta, and A. Nyska, Pulmonary thrombosis in the mouse following intravenous administration of quantum dot-labeled mesenchymal cells. Nanotoxicology 4, 98 (2010).
134. X. B. Hu, Q. H. Yue, X. Q. Zhang, X. Q. Xu, Y. Wen, Y. Z. Chen, X. D. Cheng, L. Yang, and S. J. Mu, Hepatitis B virus genotypes and evolutionary profiles from blood donors from the northwest region of China. Virol. J. 6, 199 (2009).
135. Y. Y. Hui, B. Zhang, Y. C. Chang, C. C. Chang, H. C. Chang, J. H. Hsu, K. Chang, and F. H. Chang, Two-photon fluorescence correlation spectroscopy of lipid-encapsulated fluorescent nanodiamonds in living cells. Opt. Express 18, 5896 (2010).
136. R. Fudala, S. Rout, B. P. Maliwal, T. W. Zerda, I. Gryczynski, E. Simanek, J. Borejdo, R. Rich, I. Akopova, and Z. Gryczynski, FRET enhanced fluorescent nanodiamonds. Curr. Pharm. Biotechnol. (2013).
137. A. M. Schrand, H. Huang, C. Carlson, J. J. Schlager, E. O. Sawa, S. M. Hussain, and L. Dai, Are diamond nanoparticles cytotoxic? J. Phys. Chem. B 111, 2 (2007).
138. V. Vaijayanthimala, Y. K. Tzeng, H. C. Chang, and C. L. Li, The biocompatibility of fluorescent nanodiamonds and their mechanism of cellular uptake. Nanotechnology 20, 425103 (2009).
139. Y. Xing, W. Xiong, L. Zhu, E. Osawa, S. Hussin, and L. Dai, DNA damage in embryonic stem cells caused by nanodiamonds. ACS Nano 5, 2376 (2011).
140. H. M. Kim, H. Lee, K. S. Hong, M. Y. Cho, M. H. Sung, H. Poo, and Y. T. Lim, Synthesis and high performance of magnetofluorescent polyelectrolyte nanocomposites as MR/near-infrared multimodal cellular imaging nanoprobes. ACS Nano 5, 8230 (2011).
141. X. Meng, H. C. Seton, T. Lu le, I. A. Prior, N. T. Thanh, and B. Song, Magnetic CoPt nanoparticles as MRI contrast agent for transplanted neural stem cells detection. Nanoscale 3, 977 (2011).
142. P. McColgan, P. Sharma, and P. Bentley, Stem cell tracking in human trials: A meta-regression. Stem Cell Rev. 7, 1031 (2011).
143. F. Callera and C. M. de Melo, Magnetic resonance tracking of magnetically labeled autologous bone marrow CD34+ cells transplanted into the spinal cord via lumbar puncture technique in patients with chronic spinal cord injury: CD34+ cells' migration into the injured site. Stem Cells Dev. 16, 461 (2007).
144. C. Toso, J. P. Vallee, P. Morel, F. Ris, S. Demuylder-Mischler, M. Lepetit-Coiffe, N. Marangon, F. Saudek, A. M. J. Shapiro, D. Bosco, and T. Berney, Clinical magnetic resonance imaging of pancreatic islet grafts after iron nanoparticle labeling. Am. J. Transplant. 8, 701 (2008).
145. D. Karussis, C. Karageorgiou, A. Vaknin-Dembinsky, B. Gowda-Kurkalli, J. M. Gomori, I. Kassis, J. W. Bulte, P. Petrou, T. Ben-Hur, O. Abramsky, and S. Slavin, Safety and immunological effects of mesenchymal stem cell transplantation in patients with multiple sclerosis and amyotrophic lateral sclerosis. Arch. Neurol. 67, 1187 (2010).
146. A. V. Naumova, N. Balu, V. L. Yarnykh, H. Reinecke, C. E. Murry, and C. Yuan, Magnetic resonance imaging tracking of graft survival in the infarcted heart: Iron oxide particles versus ferritin overexpression approach. J. Cardiovasc. Pharmacol. Ther. 19, 358 (2014).
147. P. Walczak, D. A. Kedziorek, A. A. Gilad, S. Lin, and J. W. Bulte, Instant MR labeling of stem cells using magnetoelectroporation. Magn. Reson. Med. 54, 769 (2005).
148. M. Neri, C. Maderna, C. Cavazzin, V. Deidda-Vigoriti, L. S. Politi, G. Scotti, P. Marzola, A. Sbarbati, A. L. Vescovi, and A. Gritti, Efficient in vitro labeling of human neural precursor cells with superparamagnetic iron oxide particles: Relevance for in vivo cell tracking. Stem Cells 26, 505 (2008).
149. A. Balakumaran, E. Pawelczyk, J. Ren, B. Sworder, A. Chaudhry, M. Sabatino, D. Stroncek, J. A. Frank, and P. G. Robey, Superparamagnetic iron oxide nanoparticles labeling of bone marrow stromal (mesenchymal) cells does not affect their "stemness." PLoS One 5, e11462 (2010).
150. S. Miyoshi, J. A. Flexman, D. J. Cross, K. R. Maravilla, Y. Kim, Y. Anzai, J. Oshima, and S. Minoshima, Transfection of neuroprogenitor cells with iron nanoparticles for magnetic resonance imaging tracking: Cell viability, differentiation, and intracellular localization. Mol. Imaging Biol. 7, 286 (2005).
151. R. Sun, J. Dittrich, M. Le-Huu, M. M. Mueller, J. Bedke, J. Kartenbeck, W. D. Lehmann, R. Krueger, M. Bock, R. Huss, C. Seliger, H. J. Grone, B. Misselwitz, W. Semmler, and F. Kiessling, Physical and biological characterization of superparamagnetic iron oxide- and ultrasmall superparamagnetic iron oxidelabeled cells: A comparison. Invest. Radiol. 40, 504 (2005).
152. E. J. van den Bos, T. Baks, A. D. Moelker, W. Kerver, R. J. van Geuns, W. J. van der Giessen, D. J. Duncker, and P. A. Wielopolski, Magnetic resonance imaging of haemorrhage within reperfused myocardial infarcts: Possible interference with iron oxide-labelled cell tracking? Eur. Heart J. 27, 1620 (2006).
153. S. C. Berman, C. Galpoththawela, A. A. Gilad, J. W. Bulte, and P. Walczak, Long-term MR cell tracking of neural stem cells grafted in immunocompetent versus immunodeficient mice reveals distinct differences in contrast between live and dead cells. Magn. Reson. Med. 65, 564 (2011).
154. A. C. Lepore, P. Walczak, M. S. Rao, I. Fischer, and J. W. Bulte, MR imaging of lineage-restricted neural precursors following transplantation into the adult spinal cord. Exp. Neurol. 201, 49 (2006).
155. C. Baligand, K. Vauchez, M. Fiszman, J. T. Vilquin, and P. G. Carlier, Discrepancies between the fate of myoblast xenograft in mouse leg muscle and NMR label persistency after loading with Gd-DTPA or SPIOs. Gene Ther. 16, 734 (2009).
156. I. Y. Chen, J. M. Greve, O. Gheysens, J. K. Willmann, M. Rodriguez-Porcel, P. Chu, A. Y. Sheikh, A. Z. Faranesh, R. Paulmurugan, P. C. Yang, J. C. Wu, and S. S. Gambhir, Comparison of optical bioluminescence reporter gene and superparamagnetic iron oxide MR contrast agent as cell markers for noninvasive imaging of cardiac cell transplantation. Mol. Imaging Biol. 11, 178 (2009).
157. J. Terrovitis, M. Stuber, A. Youssef, S. Preece, M. Leppo, E. Kizana, M. Schar, G. Gerstenblith, R. G. Weiss, E. Marban, and M. R. Abraham, Magnetic resonance imaging overestimates ferumoxide-labeled stem cell survival after transplantation in the heart. Circulation 117, 1555 (2008).
158. F. Callera and C. M. de Melo, Magnetic resonance tracking of magnetically labeled autologous bone marrow CD34+ cells transplanted into the spinal cord via lumbar puncture technique in patients with chronic spinal cord injury: CD34+ cells' migration into the injured site. Stem Cells Dev. 16, 461 (2007).
159. D. Karussis, C. Karageorgiou, A. Vaknin-Dembinsky, B. Gowda-Kurkalli, J. M. Gomori, I. Kassis, J. W. Bulte, P. Petrou, T. Ben-Hur, O. Abramsky, and S. Slavin, Safety and immunological effects of mesenchymal stem cell transplantation in patients with multiple sclerosis and amyotrophic lateral sclerosis. Arch. Neurol. 67, 1187 (2010).
160. M. D. Dunning, A. Lakatos, L. Loizou, M. Kettunen, C. Ffrench-Constant, K. M. Brindle, and R. J. Franklin, Superparamagnetic iron oxide-labeled Schwann cells and olfactory ensheathing cells can be traced in vivo by magnetic resonance imaging and retain functional properties after transplantation into the CNS. J. Neurosci. 24, 9799 (2004).
161. A. A. Gilad, P. Walczak, M. T. McMahon, H. B. Na, J. H. Lee, K. An, T. Hyeon, P. C. van Zijl, and J. W. Bulte, MR tracking of transplanted cells with "positive contrast" using manganese oxide nanoparticles. Magn. Reson. Med. 60, 1 (2008).
162. Y. Amsalem, Y. Mardor, M. S. Feinberg, N. Landa, L. Miller, D. Daniels, A. Ocherashvilli, R. Holbova, O. Yosef, I. M. Barbash, and J. Leor, Iron-oxide labeling and outcome of transplanted mesenchymal stem cells in the infarcted myocardium. Circulation 116, I38 (2007).
163. K. Shah, S. Hingtgen, R. Kasmieh, J. L. Figueiredo, E. Garcia-Garcia, A. Martinez-Serrano, X. Breakefield, and R. Weissleder, Bimodal viral vectors and in vivo imaging reveal the fate of human neural stem cells in experimental glioma model. J. Neurosci. 28, 4406 (2008).
164. Z. Li, Y. Suzuki, M. Huang, F. Cao, X. Xie, A. J. Connolly, P. C. Yang, and J. C. Wu, Comparison of reporter gene and iron particle labeling for tracking fate of human embryonic stem cells and differentiated endothelial cells in living subjects. Stem Cells 26, 864 (2008).
165. R. K. Kammili, D. G. Taylor, J. Xia, K. Osuala, K. Thompson, D. R. Menick, and S. N. Ebert, Generation of novel reporter stem cells and their application for molecular imaging of cardiacdifferentiated stem cells in vivo. Stem Cells Dev. 19, 1437 (2010).
166. G. Genove, U. DeMarco, H. Xu, W. F. Goins, and E. T. Ahrens, A new transgene reporter for in vivo magnetic resonance imaging. Nat. Med. 11, 450 (2005).
167. A. A. Gilad, M. T. McMahon, P. Walczak, P. T. Winnard, Jr, V. Raman, H. W. van Laarhoven, C. M. Skoglund, J. W. Bulte, and P. C. van Zijl, Artificial reporter gene providing MRI contrast based on proton exchange. Nat. Biotechnol. 25, 217 (2007).
168. A. A. Gilad, P. T. Winnard Jr, P. C. van Zijl, and J. W. Bulte, Developing MR reporter genes: Promises and pitfalls. NMR Biomed. 20, 275 (2007).
169. O. Zurkiya, A. W. Chan, and X. Hu, MagA is sufficient for producing magnetic nanoparticles in mammalian cells, making it an MRI reporter. Magn. Reson. Med. 59, 1225 (2008).
170. D. E. Goldhawk, C. Lemaire, C. R. McCreary, R. McGirr, S. Dhanvantari, R. T. Thompson, R. Figueredo, J. Koropatnick, P. Foster, and F. S. Prato, Magnetic resonance imaging of cells overexpressing MagA, an endogenous contrast agent for live cell imaging. Mol. Imaging 8, 129 (2009).
171. M. S. Chapekar, Tissue engineering: Challenges and opportunities. J. Biomed. Mater. Res. 53, 617 (2000).
172. A. Gao, Y. Peng, Y. Deng, and H. Qing, Potential therapeutic applications of differentiated induced pluripotent stem cells (iPSCs) in the treatment of neurodegenerative diseases. Neuroscience 228, 47 (2013).
173. D. F. Williams, On the nature of biomaterials. Biomaterials 30, 5897 (2009).
174. E. Garreta, E. Genové, S. Borrós, and C. E. Semino, Osteogenic differentiation of mouse embryonic stem cells and mouse embryonic fibroblasts in a three-dimensional self-assembling peptide scaffold. Tissue Eng. 12, 2215 (2006).
175. H. Liu and K. Roy, Biomimetic three-dimensional cultures significantly increase hematopoietic differentiation efficacy of embryonic stem cells. Tissue Eng. 11, 319 (2005).
176. S. M. Willerth, K. J. Arendas, D. I. Gottlieb, and S. E. Sakiyama-Elbert, Optimization of fibrin scaffolds for differentiation of murine embryonic stem cells into neural lineage cells. Biomaterials 27, 5990 (2006).
177. N. S. Hwang, M. S. Kim, S. Sampattavanich, J. H. Baek, Z. Zhang, and J. Elisseeff, Effects of three-dimensional culture and growth factors on the chondrogenic differentiation of murine embryonic stem cells. Stem Cells 24, 284 (2006).
178. N. S. Hwang, S. Varghese, P. Theprungsirikul, A. Canver, and J. Elisseeff, Enhanced chondrogenic differentiation of murine embryonic stem cells in hydrogels with glucosamine. Biomaterials 27, 6015 (2006).
179. L. A. Smith, X. Liu, J. Hu, and P. X. Ma, The enhancement of human embryonic stem cell osteogenic differentiation with nanofibrous scaffolding. Biomaterials 31, 5526 (2010).
180. M. V. Jose, V. Thomas, Y. Xu, S. Bellis, E. Nyairo, and D. Dean, Aligned bioactive multi-component nanofibrous nanocomposite scaffolds for bone tissue engineering. Macromol. Biosci. 10, 433 (2010).
181. S. R. Perumcherry, K. P. Chennazhi, S. V. Nair, D. Menon, and R. Afeesh, A novel method for the fabrication of fibrin-based electrospun nanofibrous scaffold for tissue-engineering applications. Tissue Eng. Part C: Methods 17, 1121 (2011).
182. N. Hild, O. D. Schneider, D. Mohn, N. A. Luechinger, F. M. Koehler, S. Hofmann, J. R. Vetsch, B. W. Thimm, R. Müller, and W. J. Stark, Two-layer membranes of calcium phosphate/collagen/ PLGA nanofibres: In vitro biomineralisation and osteogenic differentiation of human mesenchymal stem cells. Nanoscale 3, 401 (2011).
183. S. Panzavolta, M. Gioffrè, M. L. Focarete, C. Gualandi, L. Foroni, and A. Bigi, Electrospun gelatin nanofibers: Optimization of genipin cross-linking to preserve fiber morphology after exposure to water. Acta Biomater. 7, 1702 (2011).
184. R. Jayakumar, R. Ramachandran, V. V. Divyarani, K. P. Chennazhi, H. Tamura, and S. V. Nair, Fabrication of chitin-chitosan/nano TiO2 -composite scaffolds for tissue engineering applications. Int. J. Biol. Macromol. 48, 336 (2011).
185. J. N. Mackle, D. J. Blond, E. Mooney, C. McDonnell, W. J. Blau, G. Shaw, F. P. Barry, J. M. Murphy, and V. Barron, In vitro characterization of an electroactive carbon-nanotube-based nanofiber scaffold for tissue engineering. Macromol. Biosci. 11, 1272 (2011).
186. R. Langer and J. P. Vacanti, Tissue engineering. Science 260, 920 (1993).
187. E. A. Rivera and M. J. Jayo, Histological evaluation of tissue regeneration using biodegradable scaffold seeded by autologous cells for tubular/hollow organ applications. Methods Mol. Biol. 1001, 353 (2013).
188. U. A. Gurkan, S. Tasoglu, D. Kavaz, M. C. Demirel, and U. Demirci, Emerging technologies for assembly of microscale hydrogels. Adv. Healthc. Mater. 1, 149 (2012).
189. F. Xu, J. Wu, S. Wang, N. G. Durmus, U. A. Gurkan, and U. Demirci, Microengineering methods for cell-based microarrays and high-throughput drug-screening applications. Biofabrication 3, 034101 (2011).
190. P. B. Malafaya, G. A. Silva, and R. L. Reis, Natural-origin polymers as carriers and scaffolds for biomolecules and cell delivery in tissue engineering applications. Adv. Drug Deliv. Rev. 59, 207 (2007).
191. K. J. Burg, S. Porter, and J. F. Kellam, Biomaterial developments for bone tissue engineering. Biomaterials 21, 2347 (2000).
192. S. Tamura, H. Kataoka, Y. Matsui, Y. Shionoya, K. Ohno, K. I. Michi, K. Takahashi, and A. Yamaguchi, The effects of transplantation of osteoblastic cells with bone morphogenetic protein (BMP)/carrier complex on bone repair. Bone 29, 169 (2001).
193. B. Johnstone and J. Yoo, Mesenchymal cell transfer for articular cartilage repair. Expert Opin. Biol. Ther. 1, 915 (2001).
194. M. D. Krebs, R. M. Erb, B. B. Yellen, B. Samanta, A. Bajaj, V. M. Rotello, and E. Alsberg, Formation of ordered cellular structures in suspension via label-free negative magnetophoresis. Nano Lett. 9, 1812 (2009).
195. W. R. Rodriguez, N. Christodoulides, P. N. Floriano, S. Graham, S. Mohanty, M. Dixon, M. Hsiang, T. Peter, S. Zavahir, I. Thior, D. Romanovicz, B. Bernard, A. P. Goodey, B. D. Walker, and J. T. McDevitt, A microchip CD4 counting method for HIV monitoring in resource-poor settings. PLoS Med. 2, e182 (2005).
196. J. Voldman, M. L. Gray, and M. A. Schmidt, Microfabrication in biology and medicine. Annu. Rev. Biomed. Eng. 1, 401 (1999).
197. G. M. Whitesides, E. Ostuni, S. Takayama, X. Jiang, and D. E. Ingber, Soft lithography in biology and biochemistry. Annu. Rev. Biomed. Eng. 3, 335 (2001).
198. Y. S. Song, D. Adler, F. Xu, E. Kayaalp, A. Nureddin, R. M. Anchan, R. L. Maas, and U. Demirci, Vitrification and levitation of a liquid droplet on liquid nitrogen. Proc. Natl. Acad. Sci. U.S.A. 107, 4596 (2010).
199. S. M. Willerth, T. E. Faxel, D. I. Gottlieb, and S. E. Sakiyama-Elbert, The effects of soluble growth factors on embryonic stem cell differentiation inside of fibrin scaffolds. Stem Cells 25, 2235 (2007).
200. S. M. Willerth, A. Rader, and S. E. Sakiyama-Elbert, The effect of controlled growth factor delivery on embryonic stem cell differentiation inside fibrin scaffolds. Stem Cell Res. 1, 205 (2008).
201. C. E. Holy, M. S. Shoichet, and J. E. Davies, Engineering three-dimensional bone tissue in vitro using biodegradable scaffolds: Investigating initial cell-seeding density and culture period. J. Biomed. Mater. Res. 51, 376 (2000).
202. A. Khademhosseini, R. Langer, J. Borenstein, and J. P. Vacanti, Microscale technologies for tissue engineering and biology. Proc. Natl. Acad. Sci. U.S.A. 103, 2480 (2006).
203. G. T. Köse, H. Kenar, N. Hasirci, and V. Hasirci, Macroporous poly(3-hydroxybutyrate-co-3-hydroxyvalerate) matrices for bone tissue engineering. Biomaterials 24, 1949 (2003).
204. R. E. Unger, A. Sartoris, K. Peters, A. Motta, C. Migliaresi, M. Kunkel, U. Bulnheim, J. Rychly, and C. J. Kirkpatrick, Tissuelike self-assembly in cocultures of endothelial cells and osteoblasts and the formation of microcapillary-like structures on threedimensional porous biomaterials. Biomaterials 28, 3965 (2007).
205. A. J. Salgado, O. P. Coutinho, and R. L. Reis, Bone tissue engineering: State of the art and future trends. Macromol. Biosci. 4, 743 (2004).
206. U. A. Gurkan, X. Cheng, V. Kishore, J. A. Uquillas, and O. Akkus, Comparison of morphology, orientation, and migration of tendon derived fibroblasts and bone marrow stromal cells on electrochemically aligned collagen constructs. J. Biomed. Mater. Res. A 94, 1070 (2010).
207. U. A. Gurkan, J. Gargac, and O. Akkus, The sequential production profiles of growth factors and their relations to bone volume in ossifying bone marrow explants. Tissue Eng. Part A 16, 2295 (2010).
208. U. A. Gurkan, V. Kishore, K. W. Condon, T. M. Bellido, and O. Akkus, A scaffold-free multicellular three-dimensional in vitro model of osteogenesis. Calcif. Tissue Int. 88, 388 (2011).
209. U. A. Gurkan, A. Krueger, and O. Akkus, Ossifying bone marrow explant culture as a three-dimensional mechanoresponsive in vitro model of osteogenesis. Tissue Eng. Part A 17, 417 (2011).
210. E. M. Christenson, K. S. Anseth, J. J. van den Beucken, C. K. Chan, B. Ercan, J. A. Jansen, C. T. Laurencin, W. J. Li, R. Murugan, L. S. Nair, S. Ramakrishna, R. S. Tuan, T. J. Webster, and A. G. Mikos, Nanobiomaterial applications in orthopedics. J. Orthop. Res. 25, 11 (2007).
211. J. C. Adams, Cell-matrix contact structures. Cell. Mol. Life Sci. 58, 371 (2001).
212. M. Chiquet, Regulation of extracellular matrix gene expression by mechanical stress. Matrix Biol. 18, 417 (1999).
213. H. Chen, Y. Liu, Z. Jiang, W. Chen, Y. Yu, and Q. Hu, Cellscaffold interaction within engineered tissue. Exp. Cell Res. 323, 346 (2014).
214. Q. Zhang, T. Nakamoto, S. Chen, N. Kawazoe, K. Lin, J. Chang, and G. Chen, Collagen/Wollastonite nanowire hybrid scaffolds promoting osteogenic differentiation and angiogenic factorexpression of mesenchymal stem cells. J. Nanosci. Nanotechnol. 14, 3221 (2014).
215. G. R. Chaudhry, D. Yao, A. Smith, and A. Hussain, Osteogenic cells derived from embryonic stem cells produced bone nodules in three-dimensional scaffolds. J. Biomed. Biotechnol. 2004, 203 (2004).
216. S. Levenberg, J. A. Burdick, T. Kraehenbuehl, and R. Langer, Neurotrophin-induced differentiation of human embryonic stem cells on three-dimensional polymeric scaffolds. Tissue Eng. 11, 506 (2005).
217. S. Taqvi and K. Roy, Influence of scaffold physical properties and stromal cell coculture on hematopoietic differentiation of mouse embryonic stem cells. Biomaterials 27, 6024 (2006).
218. Kshitiz, D. H. Kim, D. J. Beebe, and A. Levchenko, Micro- and nanoengineering for stem cell biology: The promise with a caution. Trends Biotechnol. 29, 399 (2011).
219. T. J. Cho, L. C. Gerstenfeld, and T. A. Einhorn, Differential temporal expression of members of the transforming growth factor beta superfamily during murine fracture healing. J. Bone Miner. Res. 17, 513 (2002).
220. A. X. Le, T. Miclau, D. Hu, and J. A. Helms, Molecular aspects of healing in stabilized and non-stabilized fractures. J. Orthop. Res. 19, 78 (2001).
221. K. Tatsuyama, Y. Maezawa, H. Baba, Y. Imamura, and M. Fukuda, Expression of various growth factors for cell proliferation and cytodifferentiation during fracture repair of bone. Eur. J. Histochem. 44, 269 (2000).
222. A. Muraglia, I. Martin, R. Cancedda, and R. Quarto, A nude mouse model for human bone formation in unloaded conditions. Bone 22, 131s (1998).
223. T. Histing, P. Garcia, R. Matthys, M. Leidinger, J. H. Holstein, A. Kristen, T. Pohlemann, and M. D. Menger, An internal locking plate to study intramembranous bone healing in a mouse femur fracture model. J. Orthop. Res. 28, 397 (2010).
224. Z. Thompson, T. Miclau, D. Hu, and J. A. Helms, A model for intramembranous ossification during fracture healing. J. Orthop. Res. 20, 1091 (2002).
225. J. Handschel, K. Berr, R. Depprich, C. Naujoks, N. R. Kübler, U. Meyer, M. Ommerborn, and L. Lammers, Compatibility of embryonic stem cells with biomaterials. J. Biomater. Appl. 23, 549 (2009).
226. H. Wang, J. Zhou, Z. Liu, and C. Wang, Injectable cardiac tissue engineering for the treatment of myocardial infarction. J. Cell Mol. Med. 14, 1044 (2010).
227. L. L. Chiu, R. K. Iyer, L. A. Reis, S. S. Nunes, and M. Radisic, Cardiac tissue engineering: Current state and perspectives. Front. Biosci. 17, 1533 (2012).
228. E. Ruvinov, T. Harel-Adar, and S. Cchen, Bioengineering the infarcted heart by applying bio-inspired materials. J. Cardiovasc. Transl. Res. 4, 559 (2011).
229. J. P. Karam, C. Muscari, and C. N. Montero-Menei, Combining adult stem cells and polymeric devices for tissue engineering in infarcted myocardium. Biomaterials 33, 5683 (2012).
230. B. Guillotin, A. Souquet, S. Catros, M. Duocastella, B. Pippenger, S. Bellance, R. Bareille, M. Remy, L. Bordenave, J. Amédée, and F. Guillemot, Laser assisted bioprinting of engineered tissue with high cell density and microscale organization. Biomaterials 31, 7250 (2010).
231. N. Bursac, K. K. Parker, S. Iravanian, and L. Tung, Cardiomyocyte cultures with controlled macroscopic anisotropy: A model for functional electrophysiological studies of cardiac muscle. Circ. Res. 91, e45 (2002).
232. N. Badie, J. A. Scull, R. Y. Klinger, A. Krol, and N. Bursac, Conduction block in micropatterned cardiomyocyte cultures replicating the structure of ventricular cross-sections. Cardiovasc. Res. 93, 263 (2012).
233. C. Wang, J. A. Hennessey, R. D. Kirkton, C. Wang, V. Graham, R. S. Puranam, P. B. Rosenberg, N. Bursac, and G. S. Pitt, Fibroblast growth factor homologous factor 13 regulates Na+ channels and conduction velocity in murine hearts. Circ. Res. 109, 775 (2011).
234. T. Pong, W. J. Adams, M. A. Bray, A. W. Feinberg, S. P. Sheehy, A. A. Werdich, and K. K. Parker, Hierarchical architecture influences calcium dynamics in engineered cardiac muscle. Exp. Biol. Med. 236, 366 (2011).
235. C. de Diego, F. Chen, Y. Xie, R. K. Pai, L. Slavin, J. Parker, S. T. Lamp, Z. Qu, J. N. Weiss, and M. Valderrabano, Anisotropic conduction block and reentry in neonatal rat ventricular myocyte monolayers. Am. J. Physiol. Heart Circ. Physiol. 300, H271 (2011).
236. C. Fidkowski, M. R. Kaazempur-Mofrad, J. Borenstein, J. P. Vacanti, R. Langer, and Y. Wang, Endothelialized microvasculature based on a biodegradable elastomer. Tissue Eng. 11, 302 (2005).
237. R. K. Li, R. D. Weisel, D. A. Mickle, Z. Q. Jia, E. J. Kim, T. Sakai, S. Tomita, L. Schwartz, M. Iwanochko, M. Husain, R. J. Cusimano, R. J. Burns, and T. M. Yau, Autologous porcine heart cell transplantation improved heart function after a myocardial infarction. J. Thorac. Cardiovasc. Surg. 119, 62 (2000).
238. K. S. Masters, D. N. Shah, L. A. Leinwand, and K. S. Anseth, Crosslinked hyaluronan scaffolds as a biologically active carrier for valvular interstitial cells. Biomaterials 26, 2517 (2005).
239. H. Geckil, F. Xu, X. Zhang, S. Moon, and U. Demirci, Engineering hydrogels as extracellular matrix mimics. Nanomedicine 5, 469 (2010).
240. K. Y. Lee and D. J. Mooney, Hydrogels for tissue engineering. Chem. Rev. 101, 1869 (2001).
241. L. G. Griffith and M. A. Swartz, Capturing complex 3D tissue physiology in vitro. Nat. Rev. Mol. Cell Biol. 7, 211 (2006).
242. C. Fischbach, R. Chen, T. Matsumoto, T. Schmelzle, J. S. Brugge, P. J. Polverini, and D. J. Mooney, Engineering tumors with 3D scaffolds. Nat. Methods 4, 855 (2007).
243. M. W. Tibbitt and K. S. Anseth, Hydrogels as extracellular matrix mimics for 3D cell culture. Biotechnol. Bioeng. 103, 655 (2009).
244. S. Yang, K. F. Leong, Z. Du, and C. K. Chua, The design of scaffolds for use in tissue engineering. Part II. Rapid prototyping techniques. Tissue Eng. 8, 1 (2002).
245. M. Shin, O. Ishii, T. Sueda, and J. P. Vacanti, Contractile cardiac grafts using a novel nanofibrous mesh. Biomaterials 25, 3717 (2004).
246. R. R. Sinden, Neurodegenerative diseases. Origins of instability. Nature 411, 757 (2001).
247. C. A. Ross and M. A. Poirier, Protein aggregation and neurodegenerative disease. Nat. Med. 10, S10 (2004).
248. K. J. Barnham, C. L. Masters, and A. I. Bush, Neurodegenerative diseases and oxidative stress. Nat. Rev. Drug Discov. 3, 205 (2004).
249. E. Wong and A. M. Cuervo, Autophagy gone awry in neurodegenerative diseases. Nat. Neurosci. 13, 805 (2010).
250. E. J. Bradbury and S. B. McMahon, Spinal cord repair strategies: Why do they work? Nat. Rev. Neurosci. 7, 644 (2006).
251. S. Woerly, O. Awosika, P. Zhao, C. Agbo, F. Gomez-Pinilla, J. de Vellis, and A. Espinosa-Jeffrey, Expression of heat shock protein (HSP)-25 and HSP-32 in the rat spinal cord reconstructed with Neurogel. Neurochem. Res. 30, 721 (2005).
252. A. Wang, Z. Tang, I. H. Park, Y. Zhu, S. Patel, G. Q. Daley, and S. Li, Induced pluripotent stem cells for neural tissue engineering. Biomaterials 32, 5023 (2011).
253. Y. D. Teng, E. B. Lavik, X. Qu, K. I. Park, J. Ourednik, D. Zurakowski, R. Langer, and E. Y. Snyder, Functional recovery following traumatic spinal cord injury mediated by a unique polymer scaffold seeded with neural stem cells. Proc. Natl. Acad. Sci. U.S.A. 99, 3024 (2002).
254. S. Wu, Y. Suzuki, M. Kitada, M. Kitaura, K. Kataoka, J. Takahashi, C. Ide, and Y. Nishimura, Migration, integration, and differentiation of hippocampus-derived neurosphere cells after transplantation into injured rat spinal cord. Neurosci. Lett. 312, 173 (2001).
255. J. Xu, S. Li, F. Hu, C. Zhu, Y. Zhang, W. Zhao, T. Akaike, and J. Yang, Artificial biomimicking matrix modifications of nanofibrous scaffolds by hE-cadherin-Fc fusion protein to promotehuman mesenchymal stem cells adhesion and proliferation. J. Nanosci. Nanotechnol. 14, 4007 (2014).
256. L. Cui, J. Jiang, L. Wei, X. Zhou, J. L. Fraser, B. J. Snider, and S. P. Yu, Transplantation of embryonic stem cells improves nerve repair and functional recovery after severe sciatic nerve axotomy in rats. Stem Cells 26, 1356 (2008).
257. C. M. Hales, J. D. Rolston, and S. M. Potter, How to culture, record and stimulate neuronal networks on micro-electrode arrays (MEAs). J. Vis. Exp. 39, 2056 (2010).
258. K. Musick, D. Khatami, and B. C. Wheeler, Three-dimensional micro-electrode array for recording dissociated neuronal cultures. Lab Chip 9, 2036 (2009).
259. J. Silver and J. H. Miller, Regeneration beyond the glial scar. Nat. Rev. Neurosci. 5, 146 (2004).
260. D. H. Kim, J. Viventi, J. J. Amsden, J. Xiao, L. Vigeland, Y. S. Kim, J. A. Blanco, B. Panilaitis, E. S. Frechette, D. Contreras, D. L. Kaplan, F. G. Omenetto, Y. Huang, K. C. Hwang, M. R. Zakin, B. Litt, and J. A. Rogers, Dissolvable films of silk fibroin for ultrathin conformal bio-integrated electronics. Nat. Mater. 9, 511 (2010).
261. R. G. Wylie, S. Ahsan, Y. Aizawa, K. L. Maxwell, C. M. Morshead, and M. S. Shoichet, Spatially controlled simultaneous patterning of multiple growth factors in three-dimensional hydrogels. Nat. Mater. 10, 799 (2011).
262. N. Gulati and H. Gupta, Two faces of carbon nanotube: Toxicities and pharmaceutical applications. Crit. Rev. Ther. Drug Carrier Syst. 29, 65 (2012).
263. M. V. Park, A. M. Neigh, J. P. Vermeulen, L. J. de la Fonteyne, H. W. Verharen, J. J. Briedé, H. van Loveren, and W. H. de Jong, The effect of particle size on the cytotoxicity, inflammation, developmental toxicity and genotoxicity of silver nanoparticles. Biomaterials 32, 9810 (2011).
264. B. Yang, Q. Wang, R. Lei, C. Wu, C. Shi, Q. Wang, Y. Yuan, Y. Wang, Y. Luo, Z. Hu, H. Ma, and M. Liao, Systems toxicology used in nanotoxicology: Mechanistic insights into the hepatotoxicity of nano-copper particles from toxicogenomics. J. Nanosci. Nanotechnol. 10, 8527 (2010).
265. B. M. Prabhu, S. F. Ali, R. C. Murdock, S. M. Hussain, and M. Srivatsan, Copper nanoparticles exert size and concentration dependent toxicity on somatosensory neurons of rat. Nanotoxicology 4, 150 (2010).
266. Y. S. Chen, Y. C. Hung, L. W. Lin, I. Liau, M. Y. Hong, and G. S. Huang, Size-dependent impairment of cognition in mice caused by the injection of gold nanoparticles. Nanotechnology 21, 485102 (2010).
267. M. Ahamed, M. Karns, M. Goodson, J. Rowe, S. M. Hussain, J. J. Schlager, and Y. Hong, DNA damage response to different surface chemistry of silver nanoparticles in mammalian cells. Toxicol. Appl. Pharmacol. 233, 404 (2008).
268. M. Festag, B. Viertel, P. Steinberg, and C. Sehner, An in vitro embryotoxicity assay based on the disturbance of the differentiation of murine embryonic stem cells into endothelial cells. II. Testing of compounds. Toxicol. In Vitro 21, 1631 (2007).
269. M. V. Park, W. Annema, A. Salvati, A. Lesniak, A. Elsaesser, C. Barnes, G. McKerr, C. V. Howard, I. Lynch, K. A. Dawson, A. H. Piersma, and W. H. de Jong, In vitro developmental toxicity test detects inhibition of stem cell differentiation by silica nanoparticles. Toxicol. Appl. Pharmacol. 240, 108 (2009).
270. K. Eckardt and R. Stahlmann, Use of two validated in vitro tests to assess the embryotoxic potential of mycophenolic acid. Arch. Toxicol. 84, 37 (2010).
271. D. Napierska, L. C. Thomassen, V. Rabolli, D. Lison, L. Gonzalez, M. Kirsch-Volders, J. A. Martens, and P. H. Hoet, Sizedependent cytotoxicity of monodisperse silica nanoparticles in human endothelial cells. Small 5, 846 (2009).
272. S. Adler, C. Pellizzer, L. Hareng, T. Hartung, and S. Bremer, First steps in establishing a developmental toxicity test method based on human embryonic stem cells. Toxicol. In Vitro 22, 200 (2008).
273. D. N. Tran, L. C. Ota, J. D. Jacobson, W. C. Patton, and P. J. Chan, Influence of nanoparticles on morphological differentiation of mouse embryonic stem cells. Fertil. Steril. 87, 965 (2007).
274. T. C. Stummann, L. Hareng, and S. Bremer, Hazard assessment of methylmercury toxicity to neuronal induction in embryogenesis using human embryonic stem cells. Toxicology 257, 117 (2009).
275. A. De Smedt, M. Steemans, M. De Boeck, A. K. Peters, B. J. van der Leede, F. Van Goethem, A. Lampo, and P. Vanparys, Optimisation of the cell cultivation methods in the embryonic stem cell test results in an increased differentiation potential of the cells into strong beating myocard cells. Toxicol. In Vitro 22, 1789 (2008).
276. K. Savolainen, H. Alenius, H. Norppa, L. Pylkkänen, T. Tuomi, and G. Kasper, Risk assessment of engineered nanomaterials and nanotechnologies-a review. Toxicology 269, 92 (2010).
277. G. Oberdörster, Safety assessment for nanotechnology and nanomedicine: Concepts of nanotoxicology. J. Intern. Med. 267, 89 (2010).
278. D. B. Trout and P. A. Schulte, Medical surveillance, exposure registries, and epidemiologic research for workers exposed to nanomaterials. Toxicology 269, 128 (2010).
279. E. D. Kuempel, C. L. Geraci, and P. A. Schulte, Risk assessment and risk management of nanomaterials in the workplace: Translating research to practice. Ann. Occup. Hyg. 56, 491 (2012).
280. A. M. Fan and G. Alexeeff, Nanotechnology and nanomaterials: Toxicology, risk assessment, and regulations. J. Nanosci. Nanotechnol. 10, 8646 (2010).
281. C. Som, P. Wick, H. Krug, and B. Nowack, Environmental and health effects of nanomaterials in nanotextiles and facade coatings. Environ. Int. 37, 1131 (2011).
282. P. A. Holden, R. M. Nisbet, H. S. Lenihan, R. J. Miller, G. N. Cherr, J. P. Schimel, and J. L. Gardea-Torresdey, Ecological nanotoxicology: Integrating nanomaterial hazard considerations across the subcellular, population, community, and ecosystems levels. Acc. Chem. Res. 46, 813 (2013).
283. S. Gangwal, J. S. Brown, A. Wang, K. A. Houck, D. J. Dix, R. J. Kavlock, and E. A. Hubal, Informing selection of nanomaterial concentrations for ToxCast in vitro testing based on occupational exposure potential. Environ. Health Perspect. 119, 1539 (2011).
284. L. V. Stebounova, H. Morgan, V. H. Grassian, and S. Brenner, Health and safety implications of occupational exposure to engineered nanomaterials. Wiley Interdiscip. Rev. Nanomed. Nanobiotechnol. 4, 310 (2012).
285. M. C. Powell and M. S. Kanarek, Nanomaterial health effects-part 1: Background and current knowledge. WMJ 105, 16 (2006).
286. P. Schulte, C. Geraci, R. Zumwalde, M. Hoover, V. Castranova, E. Kuempel, V. Murashov, H. Vainio, and K. Savolainen, Sharpening the focus on occupational safety and health in nanotechnology. Scand. J. Work Environ. Health 34, 471 (2008).
287. J. S. Tsuji, A. D. Maynard, P. C. Howard, J. T. James, C. W. Lam, D. B. Warheit, and A. B. Santamaria, Research strategies for safety evaluation of nanomaterials, part IV: Risk assessment of nanoparticles. Toxicol. Sci. 89, 42 (2006).
288. K. Savolainen and H. Vainio, Health risks of engineered nanomaterials and nanotechnologies. Duodecim 127, 1097 (2011).
289. J. W. Bulte, L. Kostura, A. Mackay, P. V. Karmarkar, I. Izbudak, E. Atalar, D. Fritzges, E. R. Rodriguez, R. G. Young, M. Marcelino, M. F. Pittenger, and D. L. Kraitchman, Feridex-labeled mesenchymal stem cells: Cellular differentiation and MR assessment in a canine myocardial infarction model. Acad. Radiol. 12, S2 (2005).
290. L. Kostura, D. L. Kraitchman, A. M. Mackay, M. F. Pittenger, and J. W. Bulte, Feridex labeling of mesenchymal stem cells inhibits chondrogenesis but not adipogenesis or osteogenesis. NMR Biomed. 17, 513 (2004).
291. E. Tamariz, A. C. Wan, Y. S. Pek, M. Giordano, G. Hernández-Padrón, A. Varela-Echavarría, I. Velasco, and V. M. Castaño, Delivery of chemotropic proteins and improvement of dopaminergic neuron outgrowth through a thixotropic hybrid nano-gel. J. Mater. Sci. Mater. Med. 22, 2097 (2011).
292. S. M. Cromer Berman, Kshitiz, C. J. Wang, I. Orukari, A. Levchenko, J. W. Bulte, and P. Walczak, Cell motility of neural stem cells is reduced after SPIO-labeling, which is mitigated after exocytosis. Magn. Reson. Med. 69, 255 (2013).
293. K. Donaldson, V. Stone, C. L. Tran, W. Kreyling, and P. J. Borm, Nanotoxicology. Occup. Environ. Med. 61, 727 (2004).
294. H. M. Kipen and D. L. Laskin, Smaller is not always better: Nanotechnology yields nanotoxicology. Am. J. Physiol. Lung Cell. Mol. Physiol. 289, L696 (2005).
295. V. Stone and K. Donaldson, Nanotoxicology: Signs of stress. Nat. Nanotechnol. 1, 23 (2006).
296. D. Drobne, Nanotoxicology for safe and sustainable nanotechnology. Arh. Hig. Rada Toksikol. 58, 471 (2007).
297. BoltH.M. MarchanR. HengstlerJ.G.Nanotoxicology and oxidative stress control: Cutting-edge topics in toxicologyArch. Toxicol.