Assembly of designed oligonucleotides: A useful tool in synthetic biology for creating high-quality combinatorial DNA libraries

Methods in Molecular Biology

Volumn 1179, Issue , 2014, Pages 189-206

  Acevedo Rocha, Carlos G ^a,b   Reetz, Manfred T ^a,b  

^a MAX PLANCK INSTITUT FÜR KOHLENFORSCHUNG   (Germany)

^b PHILIPPS UNIVERSITY MARBURG   (Germany)

Author keywords

Combinatorial libraries; Degenerate homoduplex recombination; Directed evolution; DNA shuffling; Gene assembly mutagenesis; Intellectual property; Reduced amino acid alphabets; Saturation mutagenesis; Synthetic biology; Synthetic shuffling

Indexed keywords

AMINO ACID; CYTOCHROME P450 BM3; HEME; OLIGONUCLEOTIDE; UNSPECIFIC MONOOXYGENASE; PRIMER DNA;

AMINO ACID ANALYSIS; ARTICLE; ASSEMBLY OF DESIGNED OLIGONUCLEOTIDE; CODON; COMBINATORIAL LIBRARY; COMPUTER MODEL; DNA LIBRARY; IN VITRO STUDY; PRIORITY JOURNAL; SYNTHETIC BIOLOGY; DIRECTED MOLECULAR EVOLUTION; DNA SHUFFLING; GENE LIBRARY; GENETICS; POLYMERASE CHAIN REACTION; PROCEDURES;

DIRECTED MOLECULAR EVOLUTION; DNA PRIMERS; DNA SHUFFLING; GENE LIBRARY; OLIGONUCLEOTIDES; POLYMERASE CHAIN REACTION; SYNTHETIC BIOLOGY;

EID: 84925884301     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-4939-1053-3_13     Document Type: Article

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