Site-saturation mutagenesis is more efficient than DNA shuffling for the directed evolution of β-fucosidase from β-galactosidase

Journal of Molecular Biology

Volumn 352, Issue 3, 2005, Pages 621-628

  Parikh, Monal R ^a   Matsumura, Ichiro ^a  

^a EMORY UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

High throughput screen; In vitro evolution; Molecular recognition; Random mutagenesis; Substrate specificity

Indexed keywords

4 NITROPHENYLFUCOPYRANOSIDE; 4 NITROPHENYLGALACTOSIDE; BACTERIAL ENZYME; BETA FUCOSIDASE; BETA GALACTOSIDASE; PYRANOSIDE; UNCLASSIFIED DRUG;

ARTICLE; DNA SHUFFLING; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME SPECIFICITY; ESCHERICHIA COLI; GENETIC RECOMBINATION; HIGH THROUGHPUT SCREENING; IN VITRO STUDY; MOLECULAR EVOLUTION; MUTAGENESIS; NONHUMAN; PRIORITY JOURNAL; PROTEIN ENGINEERING;

ESCHERICHIA COLI;

EID: 24044554219     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jmb.2005.07.020     Document Type: Article

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