Reducing codon redundancy and screening effort of combinatorial protein libraries created by saturation mutagenesis

ACS Synthetic Biology

Volumn 2, Issue 2, 2013, Pages 83-92

  Kille, Sabrina ^a,b   Acevedo Rocha, Carlos G ^a,b   Parra, Loreto P ^a,b   Zhang, Zhi Gang ^a,b   Opperman, Diederik J ^a   Reetz, Manfred T ^a,b   Acevedo, Juan Pablo ^c  

^a MAX PLANCK INSTITUT FÜR KOHLENFORSCHUNG   (Germany)

^b PHILIPPS UNIVERSITY MARBURG   (Germany)

^c UNIVERSIDAD DE LOS ANDES   (Chile)

Author keywords

codon redundancy; combinatorial mutagenesis; directed evolution; library quality; primer degeneracy; screening effort

Indexed keywords

CYCLOHEXANONE; UNSPECIFIC MONOOXYGENASE;

AMINO ACID SEQUENCE; ARTICLE; CODON; CODON REDUNDANCY; GENE LIBRARY; GENE PROBE; GENETIC CODE; GENETIC PARAMETERS; GENETIC SCREENING; GENETIC VARIABILITY; MUTAGENESIS; NONHUMAN; PRIORITY JOURNAL; PROBABILITY; RANDOMIZATION; SATURATION MUTAGENESIS;

AMINO ACIDS; CODON; COMBINATORIAL CHEMISTRY TECHNIQUES; DNA PRIMERS; GENE LIBRARY; GENETIC CODE; MUTAGENESIS, INSERTIONAL; OXYGENASES; PROTEINS;

EID: 84874034041     PISSN: None     EISSN: 21615063     Source Type: Journal    
DOI: 10.1021/sb300037w     Document Type: Article

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