Harnessing homologous recombination in vitro to generate recombinant DNA via SLIC

Nature Methods

Volumn 4, Issue 3, 2007, Pages 251-256

  Li, Mamie Z ^a   Elledge, Stephen J ^a  

^a HOWARD HUGHES MEDICAL INSTITUTE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DNA FRAGMENT; EXONUCLEASE; RECA PROTEIN; RECOMBINANT DNA; SINGLE STRANDED DNA;

ARTICLE; CATALYSIS; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; DNA SEQUENCE; HOMOLOGOUS RECOMBINATION; IN VITRO STUDY; MOLECULAR CLONING; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; REPRODUCIBILITY;

CLONING, MOLECULAR; DNA, RECOMBINANT; GENETIC ENGINEERING; POLYMERASE CHAIN REACTION; RECOMBINATION, GENETIC; SEQUENCE HOMOLOGY, NUCLEIC ACID;

EID: 33847608289     PISSN: 15487091     EISSN: 15491676     Source Type: Journal    
DOI: 10.1038/nmeth1010     Document Type: Article

References (23)

1. Smith, H.O. & Wilcox, K.W. A restriction enzyme from Hemophilus influenzae. I. Purification and general properties. J. Mol. Biol. 51, 379-391 (1970).
2. Danna, K. & Nathans, D. Specific cleavage of simian virus 40 DNA by restriction endonuclease of Hemophilus influenzae. Proc. Natl. Acad. Sci. USA 68, 2913-2917 (1971).
3. Cohen, S.N., Chang, A.C., Boyer, H.W. & Helling, R.B. Construction of biologically functional bacterial plasmids in vitro. Proc. Natl. Acad. Sci. USA 70, 3240-3244 (1973).
4. Backman, K. & Ptashne, M. Maximizing gene expression on a plasmid using recombination in vitro. Cell 13, 65-71 (1978).
5. Hutchison, C.A. et al. Mutagenesis at a specific position in a DNA sequence. J. Biol. Chem. 253, 6551-6560 (1978).
6. Rumsby, G. An introduction to PCR techniques. PCR Methods Mol. Biol. 324, 75-89 (2006).
7. Saiki, R.K. et al. Enzymatic amplification of beta-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia. Science 230, 1350-1354 (1985).
8. Ho, S.N., Hunt, H.D., Horton, R.M., Pullen, J.K. & Pease, L.R. Site-directed mutagenesis by overlap extension using the polymerase chain reaction. Gene 77, 51-59 (1989).
9. Liu, Q., Li, M.Z., Liebham, D., Cortez, D. & Elledge, S.J. The univector plasmid fusion system, a method for rapid construction of recombinant DNA without restriction enzymes. Curr. Biol. 8, 1300-1309 (1998).
10. Hartley, J.L., Temple, G.F. & Brasch, M.A. DNA cloning using in vitro site-specific recombination. Genome Res. 10, 1788-1795 (2000).
11. Walhout, A.J. et al. GATEWAY recombinational cloning: Application to the cloning of large numbers of open reading frames or ORFeomes. Methods Enzymol. 328, 575-592 (2000).
12. Bethke, B. & Sauer, B. Segmental genomic replacement by Cre-mediated recombination: Genotoxic stress activation of the p53 promoter in single-copy transformants. Nucleic Acids Res. 25, 2828-2834 (1997).
13. Nebert, D.W., Dalton, T.P., Stuart, G.W. & Carvan, M.J. Gene-swap knock-in cassette in mice to study allelic differences in human genes. Ann. NY Acad. Sci. 919, 148-170 (2000).
14. Siegel, R.W. et al. Recombinatorial cloning using heterologous lox sites. Genome Res. 14, 1119-1129 (2004).
15. Li, M.Z. & Elledge, S.J. MAGIC: An in vivo genetic method for the rapid construction of recombinant DNA molecules. Nat. Genet. 37, 311-319 (2005).
16. Amundsen, S.K. & Smith, G.R. Interchangeable parts of the Escherichia coli recombination machinery. Cell 112, 741-744 (2003).
17. Kuzminov, A. Recombinational repair of DNA damage in Escherichia coli and bacteriophage lambda. Microbiol. Mol. Biol. Rev. 63, 751-813 (1999).
18. Konforti, B.B. & Davis, R.W. 3′ homologous free ends are required for stable joint molecule formation by the RecA and single-stranded binding proteins of Escherichia coli. Proc. Natl. Acad. Sci. USA 84, 690-694 (1987).
19. Tillett, D. & Neilan, B.A. Enzyme-free cloning: A rapid method to clone PCR products independent of vector restriction enzyme sites. Nucleic Acids Res. 27, e26 (1999).
20. Aslanidis, C. & de Jong, P.J. Ligation-independent cloning of PCR products (LIC-PCR). Nucleic Acids Res. 18, 6069-6074 (1990).
21. Haun, R.S., Servanti, I.M. & Moss, J. Rapid, reliable Ligation-independent cloning of PCR products using modified plasmid vectors. Biotechniques 13, 515-518 (1992).
22. Aslanidis, C., de Jong, P.J. & Schmitz, G. Minimal Length requirement of the single-stranded tails for ligation-independent cloning (LIC) of PCR products. PCR Methods Appl. 4, 172-177 (1994).
23. Cheo, D.L. et al. Concerted assembly and cloning of multiple DNA fragments using in vitro site-specific recombination: Functional analysis of multi-site expression clones. Gen. Res. 14, 2111-2120 (2004).